• Journal of Plant Biotechnology
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• 제48권2호
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• pp.100-105
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• 2021

Genetic engineering is a potential approach to improve secondary metabolism in plants. In order to elucidate the effect of production of anthocyanin pigment 1 (PAP1) overexpression on the bioactivity of ginseng, we analyzed its antioxidant, antimicrobial, and anti-elastase activities in this study. Our results showed that PAP1 overexpression increased the production of polyphenolic compounds including anthocyanins. The antioxidant, antimicrobial, and anti-elastase activities were stronger in anthocyanin-overproducing ginseng hairy roots (AOX) than in wild ginseng hairy roots. Using a different solvent system (0, 30, 70, and 100% (v/v) EtOH), we revealed that variations in the contents of the polyphenolic compounds were highly correlated with changes in the antioxidant and antimicrobial activities of AOX. The antioxidant, antimicrobial, and anti-elastase effects of AOX highlight genetic engineering as a powerful approach to enhance the therapeutic properties of plants. Our results show that AOX could potentially have various functional applications in the cosmetic and pharmaceutical industries.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2004년도 추계학술대회
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• pp.262-264
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• 2004

본 연구에서는 미세조류에 광스트레스를 가해서 특정 색소의 증가를 유도하였다. 광스트레스를 미세조류인 N.oculata에 각 각 가했을 때, 안세라크산틴 6.6 배 제아크산틴 8.6 배, 증가함을 HPLC로 분석하였다. 이러한 광스트레스에 노출되면 크산토필 회로에서 비올라산틴이 안세라크산틴과 제아크산틴으로 역위되어 광보호를 하기 때문이다.

• The Plant Pathology Journal
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• 제19권4호
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• pp.195-199
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• 2003

Pink root of onion occurred in the fields of the Onion Experimental Station and in the main onion cultivation area in Korea in 1998 and 1999, respectively. The casual fungus of pink root was isolated only from apricot agar. Formation of pycnidia and pycnidiospores of the fungus was highest in alternating cycles of 12 hours near ultraviolet light and 12 hours in dark condition. Its morphological characteristics and pigment formation on water agar were identical with that of Pyrenochaeta terrestris. The optimum temperature for the growth of the fungus and disease development was $25-28^{\circ}C$. When onion seeds were inoculated with the spore suspension, incubated in test-tube and sown in potted soil, disease symptoms developed in onion roots 7 and 30 days after inoculation.

• 한국토양비료학회지
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• 제43권3호
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• pp.400-406
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• 2010

서리태콩 (Glycine max Merr. var Seoritae)에서 광원에 따른 엽의 광합성 변화를 구명하기 위하여 콩의 제 1복엽이 완전 전개되었을 때 3일 동안 빛을 차단한 후 UV-B 와 일반광에 노출시켜 색소 함량과 엽록소 형광반응의 변화를 측정하였다. 암처리에서 엽록소 함량은 감소하고, 일반 광에서 회복하였다. 카로티노이드와 안토시아닌 함량은 UV-B 조사한 처리구에서 증가하였다. 엽록소 형광분석을 이용한 광합성 능률을 분석한 결과, 암처리가 진행 됨에 따라 Fv/Fm, F'v/F'm, ${\Phi}_{PSII}$ 및 NPQ는 감소하였다. 모든 변수들은 일반광에 노출되면서 회복하였으나 UV 처리한 것은 암처리 72시간의 수치와 큰 변화가 없었다. 이를 통하여 암처리 48시간 경과함으로 엽록체가 에티오플라스트로 전환되며, 일반광을 조사하였을 시 광합성 관련 광계가 복구되지만, UV-B의 강한 광이 조사되었을 때 광계가 회복되지 못하는 것으로 사료되었다.

• 대한토목학회논문집
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• 제2권2호
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• pp.11-18
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• 1982

가열혼합식(加熱混合式) 칼라포장용(鋪裝用)바인더는 스트레이트아스팔트와 물리적(物理的)인 제성질(諸性質)은 유사(類似)하면서 색깔이 흑갈색(黑褐色)이 아닐 것이 요구된다. 위의 제성질(諸性質)을 갖는 담황색(淡黃色)의 합성수지조성물(合成樹脂組成物)을 개발하여 이를 골재(骨材)와 믹싱한 혼합물(混合物)과 스트레이트아스팔트혼합물(混合物)과 각종시험(各種試驗)으로 비교한 결과 칼라포장용(鋪裝用)바인더로 사용가능성(使用可能性)을 확인(確認)했다. 색깔을 내기위한 안료(顔料) 역시 국산재료(國産材料)의 사용가능성(使用可能性)을 시험(試驗)을 거쳐 확인(確認)했다. 아스팔트 믹싱플랜트로 시험혼합(試驗混合)과 현장포설(現場鋪設)을 거쳐 실용화단계(實用化段階)에 이르게 되었다. 칼라포장용(鋪裝用) 혼합물(混合物)의 혼합(混合) 및 포설(鋪設)은 일반적(一般的)인 아스팔트콘크리트혼합물(混合物)과 같은 방식(方式)으로 하되 안료(顔料)만큼 미네랄필러분에서 차감(差減)하여 대체(代替)한다. 안료(顔料)의 배합량(配合量)은 사용(使用)하는 타재료(他材料)에 의해 시험혼합(試驗混合)에서 결정한다.

• BMB Reports
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• 제29권5호
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• pp.398-404
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• 1996

Light-chilling effects were investigated in chilling-sensitive cucumber (Cucumis sativus L. cv. Ilmichungjang) and chilling-resistant pea (Pisum sativum L. cv. Giant) leaf discs in relation to possible damage in D1 protein. In both plants, dark-chilling did not cause any noticeable changes in (Fv)m/Fm and lincomycin did not affect the decrease in (Fv)m/Fm caused by light-chilling. This result suggests that the de novo synthesis of D1 protein did not occur actively during light-chilling. In pea light-chilled for 6 h. the decreased (Fv)m/Fm was partly recovered in the dark, and almost complete recovery was observed in the light. In cucumber light-chilled for 3 h. the reduced (Fv)m/Fm decreased further for the initial 2 h recovery process in the light regardless of the treatment of lincomycin and recovered very slowly. In both plant species, the treatment of lincomycin inhibited the recovery process in the light, but did not significantly inhibit the process in the dark. In cucumber leaves pulse-labeled with $[^{35}S]Met$, the labeled band intensities of isolated pigment-protein complexes were almost the same during the 6 h light-chilling, but significant decreases in band intensities were observed during the 3 h recovery period. This result suggests that the irreversibly damaged D1 protein was degraded during the recovery period. However, no noticeable changes were observed in the pea leaves during the 12 h chilling and 3 h recovery period. The polyacrylamide gel electrophoresis of the pigment-protein complexes showed that the principal lesion sites of light-chilling were different from those of room temperature photoinhibition.

• Journal of Crop Science and Biotechnology
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• 제10권4호
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• pp.257-264
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• 2007

Rice is one of the world's staple crops and is a major source of carbohydrate. Rice is exported from several countries, providing a major source of income. There are many documents reporting that rice is a salt-sensitive crop in its developmental stages. The objective of this investigation is to evaluate the effective salt-tolerance defense mechanisms in aromatic rice varieties. Pathumthani 1(PT1), Jasmine(KDML105), and Homjan(HJ) aromatic rice varieties were chosen as plant materials. Rice seedlings photoautotrophically grown in-vitro were treated with 0, 85, 171, 256, 342, and 427 mM NaCl in the media. Data, including sodium ion$(Na^+)$ and potassium ion$(K^+)$ accumulation, osmolarity, chlorophyll pigment concentration, and the fresh and dry weights of seedlings were collected after salt-treatment for 5 days. $Na^+$ in salt-stressed seedlings gradually accumulated, while $K^+$ decreased, especially in the 342-427 mM NaCl salt treatments. The $Na^+$ accumulation in both salt-stressed root and leaf tissues was positively related to osmolarity, leading to chlorophyll degradation. In the case of the different rice varieties, the results showed that the HJ variety was identified as being salt-tolerant, maintaining root and shoot osmolarities as well as pigment stabilization when exposed to salt stress or $Na^+$ enrichment in the cells. On the other hand, PT1 and KDML105 varieties were classified as salt-sensitive, determined by chlorophyll degradation using Hierarchical cluster analysis. In conclusion, the HJ-salt tolerant variety should be further utilized as a parental line or genetic resource in breeding programs because of the osmoregulation defensive response to salt-stress.

• ALGAE
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• 제37권2호
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• pp.163-174
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• 2022

Light-emitting-diodes (LEDs) are a lighting source useful for the precise evaluation of light quality effect on biological systems. Despite the importance of light spectra on the regeneration of land plant protoplasts ("naked cells"), this factor has not been tested yet on protoplasts from multicellular algae. This study reports on the effects of pure primary colors (red, blue, and green), dichromatic (red plus blue, RB, 1 : 2) and white LEDs on protoplast regeneration from male and female Undaria pinnatifida gametophytes. We also evaluated the effect of different light spectra on pigment composition (chlorophyll a, chlorophyll c, and fucoxanthine), and the light intensities under the best condition on the regeneration process. In the early stages, blue or RB LEDs increased the percentage of dividing female protoplasts, whereas red, blue, and RB LEDs enhanced that of dividing male protoplasts. In the later stages, RB LEDs showed a positive effect only on the percentage of multiple rhizoid-like protrusions (male gametophyte). They also increased the final area of both regenerated gametophytes. The LEDs did not affect pigment composition in female gametophytes. In male gametophytes, in contrast, they reduced chlorophyll c, while blue, RB, and green LEDs decreased fucoxanthin. Under RB LEDs, the optimal light intensity was 80 µmol photons m^-2 s^-1 for female gametophytes and 40 to 60 µmol photons m^-2 s^-1 for male gametophytes. Our results suggest that dichromatic LED illumination (red-blue) improves regeneration of U. pinnatifida gametophyte-isolated protoplasts. Thus, dichromatic LEDs might a suitable light source for enhancing protoplast regeneration in brown seaweeds.

• Nutrition Research and Practice
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• 제8권5호
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• pp.550-557
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• 2014

BACKGROUND/OBJECTIVES: Angelica keiskei is a green leafy vegetable rich in plant pigment phytochemicals such as flavonoids and carotenoids. This study examined bioavailability of flavonoids and carotenoids in Angelica keiskei and the alteration of the antioxidant performance in vivo. SUBJECTS AND MATERIALS: Absorption kinetics of phytochemicals in Angelica keiskei were determined in healthy older adults (> 60 y, n = 5) and subjects with metabolic syndrome (n = 5). Subjects consumed 5 g dry Angelica keiskei powder encapsulated in gelatin capsules with a low flavonoid and carotenoid liquid meal. Plasma samples were collected at baseline, 0.5, 1, 2, 3, 4, 5, 6, 7, and 8 h. Samples were analyzed for flavonoids and carotenoids using HPLC systems with electrochemical and UV detection, respectively, and for total antioxidant performance by fluorometry. RESULTS: After ingestion of Angelica keiskei increases in plasma quercetin concentrations were observed at 1-3 and 6-8 hr in the healthy group and at all time points in the metabolic syndrome group compared to baseline (P < 0.05). Plasma lutein concentrations were significantly elevated in both the healthy and metabolic syndrome groups at 8 hr (P < 0.05). Significant increases in total antioxidant performance were also observed in both the healthy and the metabolic syndrome groups compared to baseline (P < 0.05). CONCLUSIONS: Findings of this study clearly demonstrate the bioavailability of phytonutrients of Angelica keiskei and their ability to increase antioxidant status in humans.

• Journal of Plant Biotechnology
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• 제34권1호
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• pp.19-24
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• 2007

자색고구마 (Ipomoea batatas cv. Jami) 잎 조직배양으로 유도된 캘러스는 계대배양시 생장량이 감소하였다. 빠르고 간편하게 생장하는 습관성캘러스를 $1{\mu}M\;2,4-D$ 첨가된 MSBM에서 계대배양 없이 6개월간 지속배양하여 선발하였다. 습관성캘러스는 생장조절제가 첨가되지 않는 MSBM의 고체, 액체 배지에서 계대배양 횟수의 제한 없이 빠르게 지속생장이 가능하였다. 암조건의 액체배양 6 개월 후에는 자색생합성 부위가 생겼으며, 이를 2년 여간 분리하여 자색습관성세포괴를 선발하였다. 안토시아닌 함량은 자미의 괴근에서 추출되는 1.5 mg/ml에 근접한 1.0 mg/ml으로 나타났다. 자색습관성 세포괴는 안토시아닌 기내대량에 효과적 세포주로 사용 될 수 있을 것으로 기대한다.