• Title/Summary/Keyword: plant parts

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Detection of Virus in Fruit and Seed of Vegetables Using RT-PCR (RT-PCR에 의한 과채류 열매 및 종자의 바이러스 검정)

  • 최장경;김혜자;윤주연;박선정;김두욱;이상용
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.630-635
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    • 1998
  • Tobacco mosaic tobamovirus (TMV), cucumber mosaic cucumovirus (CMV), cucumber green mottle mosaic tobamovirus (CGMMV) and zucchini yellow mosaic potyvirus (ZYMV) from individual fruits and seeds of hot pepper and cucumber were detected by the reverse transcription-polymerase chain reaction (RT-PCR). The dilution end-points for RT-PCR in curde sap from TMV. and CMV - infected hot pepper leaves and CMV - and CGMMV-infected cucumber leaves were 10-5. However, the amount of PCR product obtained from preparation of ZYMV-infected cucumber leaf was 10-fold lower than those of CMV or CGMMV-infected cucumber leaves. In hot pepper, both TMV and CMV were detected in all parts of the fruit wall tissue, but the yields of PCR products in the fruit stalk and its surrounding tissues were higher than those of the end parts of the fruit. On the other hand, in cucumber fruit infected with CMV, CGMMV or ZYMV, the fruit wall tissue and seed located in both stalk and end parts showed higher yields of PCR products than those of intermediate parts. Of five viruses that were analysed, only TMV in hot pepper seed, and CGMMV and CMV in cucumber seed were detected in testa parts.

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Procedures for Analyzing Ethylene by Gas Chromatograph (Gas Chromatograph를 이용한 에틸렌 분석 기술)

  • 이승구
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.s01
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    • pp.33-39
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    • 1989
  • Ethylene gas classified as one of five major plant hormones plays an important role in various plant metabolism. The precise analysis of ethylene production of plants or plant parts is a valuable research procedure because knowledge of ethylene production facilitates measures of the physiological activity within the tissue. This paper describes procedures for analyzing ethylene from plant tissues by gas chromatography and discusses problems associated with extracting gas samples either by introducing a vacuum to plant samples or by using a hypodermic syringe. Introduced are a continuous flow system for efficient analysis and an automated system for sampling, analyzing, calculating and recording ethylene production data.

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Outbreak of Phytophthora Rot on Pear Under Environmental Conditions Favorable to the Disease

  • Jee, Hyeong-Jin;Cho, Weon-Dae;Nam, Ki-Woong;Park, Young-Seob
    • The Plant Pathology Journal
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    • v.17 no.4
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    • pp.231-235
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    • 2001
  • From April to May 1998, Phytophthora rot on pear, which has not been reported in Korea before, became an epidemic in the southeast part of the country under abnormally higher temperature and prolonged rainy days. Average temperature was about $3^{\circ}$ higher than in normal years, and 29 days were rainy during the 2 months in the areas surveyed. Over 1,000 orchards estimated at about 270 ha in 19 cultivation areas were infected by the disease, which occurred on all parts of the tree such as leaves, shoots, branches, stems, and flower clusters. Among 43 isolates collected from various locations and plant parts, 41 were identified as Phytophthora cactorum while 2 were identified as P. cambivora based on their mycological characteristics. The representative isolates revealed strong pathogenicity not only to pear but also to apple and peach. Among 23 pear cultivars tested, 7 were estimated as susceptible, 4 were moderate, and 11 were resistant to the pathogen. Results suggest that Phytophthora disease on pear is a potential threat to pear cultivation when environmental factors are favorable to disease development.

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Effect of Metals on Anti-Oxidase Activity and Isozyme patterns in Brassica juncea

  • Jeong, Hyung-Jin;Lee, In-Jung;Sung, Mi-Hyang
    • Korean Journal of Plant Resources
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    • v.10 no.3
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    • pp.235-240
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    • 1997
  • To study the effects of metal ions on the activity of anti-oxidase enzymes, the activity of superoxide dismutase (SOD) and peroxidase (POD) and isozyme patterns of Brassica juncea have been studied after treating with CD, Cu, Zn, and Al. The activity of SOD after treating with metal ions was higher than that of untreated control. SOD activity in leaves increased by treatment of 50 ppm of Zn and 500 ppm of Al. POD in stems gave highest activity after treating with 500 ppm of Cu. When the activity was compared by plant parts, lowest POD activity was observed in leaves in which protein content was higher than other tissues. When the activity was expressed as percentage of control, SOD activity was increased after treating with metal ions. SOD activity in leaves and roots of metal treated plant was significantly increased under the metal ions stress conditions. In the roots of 50 ppm of Zn treated plant, SOD activity was extremly high. POD activity was inhibited with Cd and Zn treatment in all parts of the plant. However, in leaves and stems, there was marked increase in activity after treating with Cu. The patterns of SOD isozyme after metal treatment show that two bands were stained in all metal ion treated and that no new band appeared. POD isozyme band intensity resulting from the treatment of metal ions was in order of roots > stems > leaves, but there was no significant difference.

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Accumulation and distribution of nutrients, radionuclides and metals by roots, stems and leaves of plants

  • Huynh Truc Phuong;Vu Ngoc Ba;Bui Ngoc Thien;Loan Truong Thi Hong
    • Nuclear Engineering and Technology
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    • v.55 no.7
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    • pp.2650-2655
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    • 2023
  • In the process of growth and development, plants not only absorb essential nutritional elements, but also absorb radioactive and non-essential elements from the environment, and their distribution varies in different parts of the plant. In this study, neutron activation analysis and gamma spectrometry were performed on stems, roots, and leaves of vegetables. The results indicate that the accumulation of radionuclides and multi-elements depends on the plant type and plant parts. Activity concentrations of 226Ra and 232Th in plants were accumulated in the following order: Roots > Stems > Leaves. The highest concentrations of 40K and 210Pb were observed in the stems and leaves of plants, respectively. Essential nutrient requirements of plants are in the following order: K > Ca > Mg > Fe > Zn > Mn. Among the nonessential metals, the concentration of Na in the vegetable sample was much greater than those of the other elements. The K/Na ratio in the plant depends on the type of plant and the translocation within the plant.

Regional Physical Distribution Systems of Parts Supply for Automobile Assembly in Korea (완성차조립 부품공급의 지역적 물류체계)

  • Han, Ju-Seong
    • Journal of the Korean Geographical Society
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    • v.50 no.6
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    • pp.621-639
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    • 2015
  • The aim of this study is to consider the physical distribution system of assembly supplied from $1^{st}$ supplier and module corporation to automobile assembly plant through analysis of existing literatures and interview survey. The major findings of this study are as follows. Parts supply of automobile are to bind together with many regional parts supplier than using multi frequency and small delivery of JIT. Regional physical distribution center of joint parts and integrated physical distribution information center is to be installed in neighboring region of automobile assembly plant in order to reduce the transport cost by designating and activating multiple physical distribution and transport companies in each automobile assembly plant and turning into effective high volume shipping system.

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Chemical Constituents and Their Acetylcholinesterase Inhibitory Activity of Underground Parts of Clematis heracleifolia (조희풀(Clematis heracleifolia) 지하부의 성분과 Acetylcholinesterase억제 활성)

  • Kim, Mi Ae;Kim, Myong Jo;Chun, Wanjoo;Kwon, Yongsoo
    • Korean Journal of Pharmacognosy
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    • v.46 no.1
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    • pp.6-11
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    • 2015
  • To clarify chemical constituents of Clematis heracleifolia, isolation and structure elucidation of the underground parts of C. heracleifolia were performed. Five compounds were isolated from $CHCl_3$ and n-BuOH soluble fraction of this plant. On the basis of spectral and physico-chemical data, the structure of isolated compounds were identified as coniferyl alcohol (1), scoparone (2), (+)-lariciresinol (3), phytosterols (4), and daucosterol (5), respectively. All compounds are isolated from this plant for the first time. To evaluate anti-acetylcholinesterase activity of the isolated compounds, compounds 1, 2, 3, and 5 were tested inhibition activity against acetylcholinesterase. Among tested compounds, daucosterol (5) showed acetylcholinesterase inhibitory activity with $IC_{50}$ value of $6.1{\mu}M$.

Glycoalkaloid Content in Korean Cultivated Potato Plant and Tubers by Organ, Variety, Part and Weight (한국산 재배종 감자의 기관, 품종, 부위, 중량별 Glycoalkaloid의 변화)

  • 김정애;소궤신행;한재숙
    • Journal of the Korean Home Economics Association
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    • v.42 no.4
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    • pp.187-194
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    • 2004
  • The concentration of potato(Solanum tuberosum L.) glycoalkaloids(PCA) (i.e., ${\alpha}$-chaconine and ${\alpha}$-solanine) in Korean cultivated potato plant, and in the different varieties, parts and weights of the potato tubers, were determined by high-performance liquid chromatography(HPLC). The highest concentrations of PGA in potato plants were found in the roots, followed by the stems and leaves. A large quantity of PGA existed in the periderm of 'Atlantic' potatoes, whereas 'Irish Cobbler' contained the lowest amount in five cultivated potato tubers.'Irish Cobbler' potatoes were divided into three parts and the PGA content in each part was determined. It was found that both of the end parts of the potatoes contained higher PGA than the middle part. The PGA contents in four different sizes of potatoes increased toward the smaller size. Thesignificantly high level of PGA was contained in the smallest size potato tubers, which have been used widely for Korean cooking.

An Efficient Analysis Model for Process Quality Information in Manufacturing Process of Automobile Safety Belt Parts (자동차 안전벨트 부품 제조공정에서의 효율적 공정품질정보 분석 모형)

  • Kong, Myung Dal
    • Journal of the Korean Institute of Plant Engineering
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    • v.23 no.4
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    • pp.29-38
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    • 2018
  • Through process quality information, the time required for process quality analysis has been drastically shortened, the process defect rate has been reduced, and the manufacturing lead time has been shortened and the on-time delivery rate has been improved. Therefore, The purpose of this study is to develop a quality information analysis system model that effectively shortens the time required for process quality analysis in automobile safety belt parts manufacturing process. As a result of experiments on communication operation between manufacturing execution system (MES) quality server, injection machine control computer, injection machine programmable logic controller (PLC) and terminal, in analyzing quality information, the conventional handwriting input method took an average of 20 minutes, but the new multi-network method took about 2 minutes on average. In addition, the process defect rate was reduced by 13% and the manufacturing lead time was shortened from 28 hours to 20 hours. The delivery compliance rate improved from 96 to 99%.