• Title/Summary/Keyword: plant morphology

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Effect of Benzyladenopurine Concentration on Growth and Morphology of Soybean Sprouts and Comparison with Selling Products (BA 처리농도에 따른 콩나물의 생장과 시판 콩나물과의 비교)

  • 강진호;조용준;전병삼;윤수영;전승호;김희규
    • Korean Journal of Plant Resources
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    • v.17 no.2
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    • pp.94-101
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    • 2004
  • Commercial value of soybean sprouts should be affected by their morphology including the lateral roots. This study was carried out to determine the effect of benzyladenopurine (BA) concentration on growth and morphology of soybean sprouts in order to compare them with the selling products collected from the markets. Four cultivars (cv. Eunhakong, Pungsannamulkong, Sowonkong, and Junjery) were cultured for 6 days after imbibed for 5 hours into different BA solutions (0, 1,2,4, and 8 ppm). On the 6th day, hypocotyl length was measured to calculate the composition rate of > 7 cm (A), 4 to 7 cm (B), < 4 cm (C) and non-germination (D), and the ratio of hypocotyl to root length (H/R ratio) on the base of hypocotyl length as well as lateral root, hypocotyl diameter, and fraction fresh and dry weights. The composition rates classified with hypocotyl length showed big differences between 4 cultivars in class A and C; Class A, in which cv. Sowonkong and Junjery had higher rates than cv. Eunhakong and Pungsannamulkong, was decreased with BA concentration of higher than 4 ppm, but class C showed the reverse result to class A. Any lateral root was not formed in higher than 4 ppm BA solutions although fewer in cv. Pungsannamulkong and Junjery than in the two other cultivars. Hypocotyl and root lengths were decreased with increased BA concentrations, and in all BA concentration, hypocotyl length of cv. Junjery was the longest. Hypocotyl diameter, hypocotyl and total fresh weights were thicker and heavier in 2 to 4 ppm BA concentrations than in the other ones, although in case of BA application, cv, Eunhakong and Sowonkong were heavier. The H/R ratios were increased with increased BA concentration. The ratios of nearly all the selling products ranged from 1 to 2 ppm when compared to them from our experiment.

Phylogeny of the family Ophioglossaceae with special emphasis on genus Mankyua (제주고사리삼을 중심으로한 고사리삼과 식물의 계통)

  • Sun, Byung-Yun;Baek, Tae Gyu;Kim, Young-Dong;Kim, Chan Soo
    • Korean Journal of Plant Taxonomy
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    • v.39 no.3
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    • pp.135-142
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    • 2009
  • Phylogeny of the family Ophioglossaceae and a phylogenetic position of Mankyua were estimated through analyses of chloroplast rbcL gene sequences and spore morphology. Sequence analysis of the rbcL gene clearly indicated that there are two major lineages in the family Ophioglossaceae: Botrychioid lineage and Ophioglossoid lineage. The Botrichioid lineage is composed of three distinct clades: Botrychium, Helminthostachys and Mankyua, where Helminthostachys and Mankyua were placed as sister groups to the Botrychium. Within the genus Botrychium, subgenera Septridium and Botrychium were monophyletic, while taxa of subgen. Botrypus branched as sister of the two, successively, thus making a non-monophyletic group. Ophioglossum formed the Ophioglossoied lineage, where the subgen. Ophioglossum is monophyletic, while subgen. Cheiroglossa and Ophoderma formed a sister relationship with subgen. Ophioglossum. In terms of external morphology and spores, Mankyua is most similar to Helminthostachys, however, patristic distance in the cladogram and trophophore characteristics of the two genera are distinct. Therefore, Mankyua is a well defined genus within the family in terms of morphology as well as molecular phylogeny which places it in basal position of the Botrychioid lineage on the gene tree.

In-vitro Anticancer and Antioxidant Activity of Gold Nanoparticles Conjugate with Tabernaemontana divaricata flower SMs Against MCF -7 Breast Cancer Cells

  • Preetam Raj, J.P;Purushothaman, M;Ameer, Khusro;Panicker, Shirly George
    • Korean Chemical Engineering Research
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    • v.54 no.1
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    • pp.75-80
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    • 2016
  • Biologically stabilized gold nanoparticles were synthesized from the flower aqueous extract of T. divaricata. The synthesized nanoparticles were characterized by UV-Vis spectrophotometer, Zeta sizer, FTIR and TEM analysis. T. divaricata reduced gold nanoparticles having particle size and potential of 106.532 nm and -10.2 mV, respectively, with a characteristic peak of 550 nm in UV-visible spectrophotometer. FTIR graph after comparison between the crude flower extract and gold nanoparticles showed three major shifts in the functional groups. The morphology and size of the gold nanoparticles were examined by HRTEM analysis, which showed that most of the nanoparticles were nearly spherical with size of 100 nm. The gold nanoparticles synthesized demonstrated potent anticancer activity against MCF-7 cell line. The findings conclude that the antioxidant molecule present in T. divaricata may be responsible for both reduction and capping of gold nanoparticles which possess potential applications in medicine and pharmaceutical fields.

Morphological Traits of S598A Sweetpotato as an Industrial Starch Crop

  • Kim, Kyung-Moon;Kim, Ji-Yeon;Kim, Jung-Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.4
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    • pp.422-426
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    • 2009
  • Sweetpotato is one of the important starch crops, current more considered as an industrial crop rather than food because it has higher starch content (over 80% of biomass), it is used for bio resources for industrial area. In this study, we generated S598A (a mutant gene of oat phytochrome A) sweetpotato plant using Agrobacterium-transformation method. Morphological characteristics of S598A plant were compared with the wild type sweetpotato, S598A had darker green leaves, increased chlorophyll content higher than to two-fold, delayed leaf senescence, shorter plant height (60% shorter than that of the wild type), more number of leaves and petioles about 1.8-fold, shorter petiole length (30% shorter), 1.2-fold more branches and 1.6-fold thicker stem diameters. From this study, S598A plants with such phenotypic characteristics might be able to use the solar energy efficiently, to have increased tolerance to biotic and abiotic stresses and finally to increase productivity (not only starch yield but also root biomass yield). S598A sweetpotato lines are under field trials.

Identification and Characterization of Pathogenic and Endophytic Fungal Species Associated with Pokkah Boeng Disease of Sugarcane

  • Hilton, Angelyn;Zhang, Huanming;Yu, Wenying;Shim, Won-Bo
    • The Plant Pathology Journal
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    • v.33 no.3
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    • pp.238-248
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    • 2017
  • Pokkah Boeng is a serious disease of sugarcane, which can lead to devastating yield losses in crop-producing regions, including southern China. However, there is still uncertainty about the causal agent of the disease. Our aim was to isolate and characterize the pathogen through morphological, physiological, and molecular analyses. We isolated sugarcane-colonizing fungi in Fujian, China. Isolated fungi were first assessed for their cell wall degrading enzyme capabilities, and five isolates were identified for further analysis. Internal transcribed spacer sequencing revealed that these five strains are Fusarium, Alternaria, Phoma, Phomopsis, and Epicoccum. The Fusarium isolate was further identified as F. verticillioides after Calmodulin and EF-$1{\alpha}$ gene sequencing and microscopic morphology study. Pathogenicity assay confirmed that F. verticillioides was directly responsible for disease on sugarcane. Co-inoculation of F. verticillioides with other isolated fungi did not lead to a significant difference in disease severity, refuting the idea that other cellulolytic fungi can increase disease severity as an endophyte. This is the first report characterizing pathogenic F. verticillioides on sugarcane in southern China.

Effect of Embryo Morphology on Plant Development in Anther Cultures of Paeonia lactiflora Pall. (작약(Paeonia Lactiflora Pall.)의 약배양에서 형성된 배의 형태가 유식물 발달에 미치는 영향)

  • 손재근;권용삼;김경민
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.165-168
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    • 1995
  • The embryos formed from anther culture of peony exhibited divergent morphologies. The frequency of normal embryos with two cotyledons was about two times higher in the embryos formed through direct embryogenesis than those formed from callus. About 69% of the embryos with two cotyledons converted into normal plants, but convention rate of the abnormal embryos with one and three or four cotyledons was only 4 to 9%. The embryos of hem and bowling pin shape did not undergo development into normal plants.

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Environment and Ecological Characteristics in Euphorbia ebracteolata Hayata Flora (붉은대극의 자생지 환경과 생태적 특성)

  • Ahn, Byung-Tae;Kim, Jae-Gil;Ro, Jai-Seup;Park, In-Keun;Lee, Kyong-Soon
    • Korean Journal of Medicinal Crop Science
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    • v.5 no.1
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    • pp.28-35
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    • 1997
  • Biosystematic study on Euphorbia ebracteolata Hayata was carried out by comparing ecology and morphology between wild and cultivated ones. As the result of this study, cultivation method about this medicinal plant was established, and ecologically, the shape of leaf or root of this plant appeared to be dependent on ecological environment while the hair of ovary and the involucel were considered to be fixed characters. From the investigation of natural conditions of habitats, this plant was considered to be a facultive sciofytes and a day neutral plant.

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Taxonomic reexamination of two Huperzia species (Lycopodiaceae) in Northeast Asia (동북아시아 뱀톱속 (석송과) 두 종의 분류학적 재검토)

  • Lim, Jina;Yun, Na Rae;Lee, Byoung-yoon;Sun, Byung-Yun
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.299-305
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    • 2015
  • A comparison of the external morphology and spores clearly distinguished Huperzia lucidula (Michaux) Trevis. in northeast Asia and North America in terms of leaf shape, leaf margin, distribution of stomata on leaves, and spore shape. Therefore, the northeast Asian plants should be treated as a separate species, H. asiatica (Ching) B.-Y. Sun & J. Lim. In addition, we believe that H. javanica (Sw.) C. Yang, regarded as conspecific or infraspecific with H. serrata (Thunb.) Trevis., is a distinct species based on the presence of a leaf petiole and serrated leaf margins, and the shape of the gemmiferous branchlets.

A taxonomic study of the genus Dioscorea L. (Dioscoreaceae) in Korea based on morphological characters (한국산 마속(마과)의 외부형태형질에 의한 분류학적 연구)

  • Chung, Dae Hee;Chung, Gyu Young
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.380-390
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    • 2015
  • The morphological characters were observed in six taxa in the genus Dioscorea of Korea (D. polystachya, D. japonica, D. nipponica, D. quinqueloba, D. tenuipes, and D. dokoro). The underground structure and the formation of bulbil were useful characters for separating sect. Enanatiophyllum from sect. Stenophora. The important characters for discriminating taxonomic entities were as follows: the position of the anther and the shape of the pistil in the microstructural characters, including the protuberance of the base of the petiole, the shape and color of the flower, the shape of the fruit, and the shape of the seed wing. Based on the results, a key to Korean Dioscorea is provided and the distribution of these species in Korea is described.

Induction of Symbiosis between Nostoc muscorum and Cultured Plant Cells II. Changes of nitrogen fixation ability and morphology by association of N. muscorum with cultured tobacco cells (Nostoc muscorum과 식물배양세포의 공생유도에 관한 연구 II. N. muscorum과 담배배양세포의 혼합배양에 따른 질소고정능과 형태적 변화)

  • 정현숙
    • Journal of Plant Biology
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    • v.30 no.4
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    • pp.257-266
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    • 1987
  • Investigation on the ability of nitrogen usage by N2-fixing Nostoc muscorum and cultured tobacco cells when they were associately cultured on nitrogen-free media was carried out. Also, effect of polyamines on the associated cultured condition was carried out. In addition, morphological changes of N. muscorum and cultured tobacco cells in associate culture were observed to detect the possibility of induction of nitrogen fixing ability on cultued plant cells. The activity of nitrogenase increased markedly when N. muscorum was grown exclusively on nitrogen-free media. When N. muscorum was cultured associately with cultured tobacco cells on nitrogen-free media containing polyamines, high activity was detected in 10-4 M spermine treated group. Investigation on the change of polyamine amounts showed two times increase in spermidine and eight times increase in spermine on a associate culture. These effects of associated culture were shown through morphological change such as dense loclization of N. muscorum around the cultured tobacco cells as well as inside the cells. These results indicate the viability of N. muscorum in cultured tobacco cells and possible induction of nitrogen fixation ability by symbiosis.

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