• 제목/요약/키워드: plant functional genomics

검색결과 142건 처리시간 0.033초

Comparison of Adventitious Shoot Formation in Petiole Explant Cultures of 20 Cultivars of Catharanthus roseus

  • Lee, Soo-Young;Park, Pil-Son;Chung, Hwa-Jee;In, Dong-Soo;Park, Dong-Woog;Jang R. Liu
    • Journal of Plant Biotechnology
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    • 제5권1호
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    • pp.59-61
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    • 2003

  • Petiole explants from 20 cultivars of Catharanthus roseus were cultured on various shoot-inducing media to assess their competence for adventitious shoot formation. After eight weeks of culture on Murashige and Skoog' s medium supplemented with 4.4 $\mu\textrm{m}$6-benzyladenine and 0.5 $\mu\textrm{m}$ $\alpha$-naphthaleneacetic acid, petiole explants from 'Cooler Icy Pink' exhibited the greatest frequency of adventitious shoot formation at 40%, which was followed by 'Little Bright Eye'. By comparing with a previous study on assessment of competence for adventitious shoot formation in hypocotyl explant cultures of various cultures, it is indicated that the relative degree of their competence among cultivars varies to the organ used for the source of explant. Excised adventitious shoots were readily rooted on half-strength MS basal medium. Regenerated plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.

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The Rice FON1 Gene Controls Vegetative and Reproductive Development by Regulating Shoot Apical Meristem Size

  • Moon, Sunok;Jung, Ki-Hong;Lee, Do-Eun;Lee, Dong-Yeon;Lee, Jinwon;An, Kyungsook;Kang, Hong-Gyu;An, Gynheung
    • Molecules and Cells
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    • 제21권1호
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    • pp.147-152
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    • 2006

  • Most plant organs develop from meristems. Rice FON1, which is an ortholog of Clv1, regulates stem cell proliferation and organ initiation. The point mutations, fon1-1 and fon1-2, disrupt meristem balance, resulting in alteration of floral organ numbers and the architecture of primary rachis branches. In this study, we identified two knockout alleles, fon1-3 and fon1-4, generated by T-DNA and Tos17 insertion, respectively. Unlike the previously isolated point mutants, the null mutants have alterations not only of the reproductive organs but also of vegetative tissues, producing fewer tillers and secondary rachis branches. The mutant plants are semi-dwarfs due to delayed leaf emergence, and leaf senescence is delayed. SEM analysis showed that the shoot apical meristems of fon1-3 mutants are enlarged. These results indicate that FON1 controls vegetative as well as reproductive development by regulating meristem size.

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21세기 식물생명공학과 생물산업의 전망: 유전체 연구에 의한 Paradigm Shift (Prospects for Plant Biotechnology and Bioindustry in the 21st Century: Paradigm Shift Driven by Genomics)

  • 유장렬;최동욱;정화지
    • 한국식물생명공학회:학술대회논문집
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    • 한국식물생명공학회 2002년도 춘계학술대회
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    • pp.19-25
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    • 2002

  • Biotechnology in the 21st century will be driven by three emerging technologies: genomics, high-throughput biology, and bioinformatics. These technologies are complementary to one another. A large number of economically important crops are currently subjected to whole genome sequencing. Functional genomics for determining the functions of the genes comprising the given plant genome is under progress by using various means including phenotyping data from transgenic mutants, gene expression profiling data from DNA microarrays, and metabolic profiling data from LC/mass analysis. The aim of plant molecular breeding is shifting from introducing agronomic traits such as herbicide and insect resistance to introducing quality traits such as healthful oils and proteins, which will lead to improved and nutritional food and feed products. Plant molecular breeding is also expected to aim to develop crops for producing human therapeutic and industrial proteins.

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