• Korean Journal of Plant Tissue Culture
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• v.22 no.2
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• pp.71-76
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• 1995

The effects of light on the activities of several antioxidative enzymes, catalase (CAT), superoxide dismutase(SOD), ascorbate oxidase(AO), and peroxidase(POD) were examined in the hairy root cultures of Phytolacca esculenta van Houtte induced by Agrobacterium tumefaciens $A_4$T. Activities of CAT, SOD, and AO were significantly decreased with incresing light intensity (500-2,000 lx). The activity of AO under high light condition (2,000 lx)was decreased by 92% compared to the dark condition. The activities of glutathoine peroxidase (GPO), ascorbate peroxidase (APO) and general POD were increased under lower light intensify below 500 lx. The activity of GPO under 2,000 lx was decreased by 85% compared to the dark condition. The activities of antioxidative enzymes were more decreased in blue light (400-500nm). The activities of antioxidative enzymes in blue light intensity were increased in lower light intensity below 30 lx, but decreased 21-70% under 200 lx. The activity of AO was decreased by 70% under 200 lx with increasing blue light intensity. Our results suggest that the activities of antioxidative enzymes in hairy roots might be inhibited by endogenous oxidants generated under the high blue light conditions.

• Korean Journal of Pharmacognosy
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• v.26 no.3
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• pp.227-238
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• 1995

Recent reports on biotransformation of monoterpene alchols, aldehydes, acetates and epoxides are summerized. The studies have focused on stereospecific reaction of the functional groups of exogenous foreign substrates by foreign plant cells and micro-organisms. An another important aspect of research is the development of the immobilization technique for cells or related enzymes.

• 한국전자현미경학회:학술대회논문집
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• 2003.05a
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• pp.39-39
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.183-183
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• 2003

• Journal of environmental and Sanitary engineering
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• v.10 no.2
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• pp.74-78
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• 1995

Several reports have suggested that these hydrolases( chitlnase, glucanase ) are likely volved in defense reactions in this Plant. In this paper, Induction by ethylene, mechanism, properties and function for Activation of these enzymes were Summarized.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.58-59
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• 2000

• Journal of Korea Soil Environment Society
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• v.2 no.1
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• pp.3-12
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• 1997

Phytoremediation, using plants to remediate toxic organic and inorganic pollutants in contaminated soils, is an emerging technology for environmental cleanup. Three strategies of this technology are applicable to the remediation of toxic heavy metals, radionuclides, and toxic organic pollutants: They are (1) phytoextraction, in which plants anumulate the contaminants and are harvested for the downstream processing; (2) phytodegradation, in which plant-released enzymes or plant-associated microorganisms convert toxic pollutants into non-toxic materials; and (3) phytostabilization, in which toxic pollutants are precipitated from solution or absorbed in either the plant tissue or the soil matrix. Phytoremediation is more effective and less expensive than other current treatment technologies.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.265-269
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• 1999

Transgenic tobacco plants expressing either a sweet potato anionic peroxidase (POD) (swpal) or neutral POD (swpnl) were irradiated by gamma radiation, and the gamma radiation-induced biochemical changes in antioxidant enzymes and plant growth inhibition were investigated at 30 days after treatment. Gamma radiation significantly inhibited the growth of all plants regardless of transgenic or nontransformed plants, showing a dose-dependent inhibition. In high dosage of 50 and 70 Gy, plant heights were severely retarded and new leaves does not emerged. No significant changes in antioxidant enzymes such as POD, superoxide dismutase and catalase were observed in all plants regardless of irradiation dosages ranging from 10 to 50 Gy. These results suggest that sweet potato PODs may be not involved in the protection against the oxidative stress induced by gamma radiation.

• Mycobiology
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• v.39 no.4
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• pp.290-298
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• 2011

The ability of benzothiadiazole (BTH) and/or humic acid (HA) used as seed soaking to induce systemic resistance against a pathogenic strain of Fusarium oxysporum was examined in four soybean cultivars under greenhouse conditions. Alone and in combination the inducers were able to protect soybean plants against damping-off and wilt diseases compared with check treatment. These results were confirmed under field conditions in two different locations (Minia and New Valley governorates). The tested treatments significantly reduced damping-off and wilt diseases and increased growth parameters, except the number of branches per plant and also increased seed yield. Application of BTH (0.25 g/L) + HA (4 g/L) was the most potent in this respect. Soybean seed soaking in BTH + HA produced the highest activities of the testes of oxidative enzymes followed by BTH in the four soybean cultivars. HA treatment resulted in the lowest increases of these oxidative enzymes. Similar results were obtained with total phenol but HA increased total phenol more than did BTH in all tested cultivars.

• Korean Journal Plant Pathology
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• v.11 no.3
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• pp.258-264
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• 1995

Production and some properties of chitinolytic enzymes were investigated by 80% ammonium sulfate precipitates (crude enzymes) from culture supernatant of antagonistic bacteria, Chromobacterium violaceum strain C-61 and strain C-72, Aeromonas hydrophila, Aeromonas caviae, and Serratia marcescens. The maximum production of chitinase was obtained from the 3-day culture at 28$^{\circ}C$ in C. violaceum stains, the 6-day culture in S. marcescens, and the 2-day culture in A. hydrophila and A. caviae. In the optimum culture periods, chitinase activity of C. violaceum strains C-61 was 1.5, 5.5, 12.0 and 11.3 times higher than those of strain C-72, S. marcescens, A. hydrophila and A. caviae, respectively. However, N,N'-diacetylchitobiase activity was 3.2 times higher in S. marcescens than in C. violaceum strain C-61, and that of Aeromonas spp.was very low. On gels containing glycol chitin, chitinase of C. violaceum strains showed four isoforms of 54-, 52-, 50- and 37-kDa, whereas there were four isoforms of 58-, 52-, 48- and 38-kDa in S. arcescens, three isoforms of 70-, 58- and 54-kDa in A. hydrophila and six isoforms of 90-, 79-, 71-, 63-, 58- and 38-kDa in A. caviae. The chitinase of C. violaceum strain C-61 was most active at pH 7.0 and at 5$0^{\circ}C$ and was stable in ranges of pH 5.0~10.0 for 2 hours and of 0~5$0^{\circ}C$ for 30 min.