• Title/Summary/Keyword: plant care

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Development of transgenic disease-resistant root stock for the growth of watermelon

  • Cho, Song-Mi;Chung, Soo-Jin;Moon, Sun-Jin;Kim, Kwang-Sang;Kim, Young-Cheol;Cho, Baik-Ho
    • Proceedings of the Korean Society of Plant Biotechnology Conference
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    • 2004.10a
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    • pp.62-65
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    • 2004
  • To protect the watermelon against soil-borne pathogens, we are currently producing disease-resistant transgenic root stock for the growth of watermelon, A defensin gene (J1-1) from Capsicum annum, a ACC deaminase gene from Pseudomonas syringae, a galactinol synthase (CsGolS) gene from Cucumis sativus, and a WRKY (CvWRKY2) gene from Citullus vulgaris were used as transgenes for disease resistance. The gene were transformed into a inbred line (6-2-2) of watermelon, Kong-dae watermelon and a inbred line (GO702S) of gourd, respectively, by Agrobacterium-mediated transformation. Putative transgenic plants were selected in medium containing 100mg/L kanamycin, and then integration of the genes into the genomic DNA were demonstrated by PCR analysis. Successful integration of the gene in regenerated plants was also confirmed by PCR (Figf 1), genomic Southern blot (Fig 2), RT-PCR (Fig 3), and Northern blot analysis(Fig 4). Several T1 lines having different transgene were produced, and disease resistance of the T1 lines are under estimation.

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Workers' Experiences in Shift Work (근로자들의 교대근무 경험)

  • Kim, Young-Hea;Kim, Young-Mi;Koo, Mi-Jee;Kim, So-Hee;Lee, Nea-Young;Chang, Koung-Oh
    • Research in Community and Public Health Nursing
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    • v.18 no.2
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    • pp.284-292
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    • 2007
  • Purpose: The purpose of this study is to explore and understanding the nature of the shift workers' experience in industries requiring 24 hours-a-day service provision and support. For the Purpose, the present researcher made a research Question, "What are workers' experience in the shift work?" Methods: In the study, 5 male shift workers, 3 female shift worker and a woman (key informant) whose husband is a shift worker participated. Then profound interviews with the participants were made after their agreement. Results: The researcher classified the significant statements under 6 theme clusters, 1) sense of difference; 2) worries about health; 3) draining of emotion; 4) anxiety; 5) sadness; and 6) being comfortable. Conclusions: Shift work which is inevitable in modern society may have severe influences on shift workers' physical, mental and psychological aspects by causing their work cycle rhythm to be unbalanced. Finally, the researcher hopes that the results of the study would help understand workers' life and increase social concern and support to the workers.

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Legalization of Tree Doctor System and the Role of KSPP (나무의사 제도 법제화에 따른 식물병리학회의 역할)

  • Cha, Byeongjin
    • Research in Plant Disease
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    • v.23 no.3
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    • pp.207-211
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    • 2017
  • In December of 2016, 'The Forest Protection Act' was amended partly in The National Assembly and the socalled 'Tree Doctor Act' was promulgated. Tree Doctor Act will be enforced from June 28, 2018. Under the new Act, none other than 'Tree Hospital' can do disease and pest management work for trees in public living space. The only exclusive qualification for tree hospital is a 'Tree Doctor', the government registered license which is newly established by the Act. To become a tree doctor, he/she must complete the tree doctor training courses in the designated 'Tree Doctor Academy' and pass the qualification test. Currently, Korea Forest Service is drafting the enforcement ordinances and regulations for the implement of Tree Doctor Act. When taking into consideration that the most fundamental and important discipline of the plant and tree health care is the plant pathology, and that the tree health care is a promising business for young plant pathology people, Korean Society of Plant Pathology is ought to be actively involved in the preparation of the enforcement ordinances and regulations, and help the early establishment of the new tree health care system in living spaces of Korea.

Development of transgenic disease-resistance root stock for growth of watermelon.(oral)

  • S.M. Cho;Kim, J.Y.;J.E. Jung;S.J. Mun;S.J. Jung;Kim, K.S.;Kim, Y.C.;B.H. Cho
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.65.2-65
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    • 2003
  • To protect the plant against several soil-borne pathogens, we are currently constructing disease-resistant transgenic root stock for the growth of cucurbitaceae vegetable plants, watermelon and gourd. We made a watermelon cDNA library from Cladosporium cucumerinum-Infected leaves for substractive hybriazation and differential screening. We isolated the several pathogen inducible cDNA clones, such as caffeoyl-CoA-methyltransferase, LAA induced protein, receptor-like kinase homolog, hydroxyproline-rich glycoprotein, catalase, calmodulin binding protein, mitochondrial ATPase beta subunit, methyl tRNA synthetase and WRKY transcription factors. We previously obtained CaMADS in pepper and galactinol synthase ( CsGolS) in cucumber that were confirmed to be related with disease-resistance. CaMADS and CsGolS2 were transformed into the inbred line 'GO701-2' gourd, the inbred line '6-2-2' watermelon and the Kong-dye watermelon by Agrobacterium tumerfaciens LBA4404. Plant growth regulators (zeatin, BAP and IAA) were used for shoot regeneration and root induction for optimal condition. Putative transgenic plants were selected in medium containing 100mg/L kanamycin and integration of the CaMADS and CsGO/S2 into the genomic DNA were demonstrated by the PCR analysis. We isolated major soil-borne pathogens, such as Monosporascus cannonballus, Didymella bryoniae, Cladosporium cuvumerinum from the cultivation area of watermelon or root stock, and successfully established artificial inoculation method for each pathogen. This work was supported by a grant from BioGreen 21 program, Rural Development Administration, Republic of Korea.

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The Use of Plants in Indigenous Health Care Practice of the Hajong Tribe Community in North Eastern Bangladesh

  • Rana, Md. Parvez;Sohel, Md. Shawkat Islam;Akhter, Sayma;Hassan, Mohammad Rakibul
    • Journal of Forest and Environmental Science
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    • v.25 no.1
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    • pp.25-33
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    • 2009
  • An ethno-medical investigation was carried out to understand the use of plants in indigenous health care practice of the Hajong tribe community in North Eastern Bangladesh. The study of ethnobotany relating to any tribe is in itself a very intricate or long-winded process. The present study was done through structured questionnaires in consultations with the tribal practitioners and has resulted in the documentation of 25 plant species belonging to 21 families including herbs (36%), shrubs (32%), trees (28%) and climber (4%) were frequently used by the Hajong tribe for curing 27 ailments. For curing ailments, the use of aboveground plant parts was higher (86.67%) than the underground plant parts (13.33%). Leaf was used in the majority of cases for medicinal preparation (13 species), followed by fruits (7 species), root/rhizome (4 species), whole plant (3 species) and besides these, seed, latex and bark were used one species each, respectively. The study thus underlines the potentials of the ethnobotanical research and the need for the documentation of indigenous healthcare knowledge pertaining to the medicinal plant utilization for the greater benefit of mankind.

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The anti-hyperglycemic property of different ginseng partitions

  • Xie, Jing-Tian;Wang, Chong-Zhi;Kim, Stephen;Yuan, Chun-Su
    • Advances in Traditional Medicine
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    • v.5 no.1
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    • pp.1-15
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    • 2005
  • Ginseng is a popular medicinal plant highly valued throughout the world. Asian ginseng is one of the most common species of ginseng. It has long occupied a significant position in oriental medicine and has been justified its name as the 'king herb'. As a nutritional supplement, ginseng is an extremely common and popular herbal medicine in the United States and Canada in recent decades. The multiple constituents of ginseng possess equally multifaceted pharmacological actions as demonstrated by numerous studies. Ginseng root and its constituents influenced the central nervous system, endocrine, cardiovascular, gastrointestinal system, sexual, renal organ and immune system, etc. One important action is its anti-hyperglycemic effect. Previous studies on ginseng demonstrate that only the root of ginseng has been used in the treatment of diabetes, while the other parts of ginseng plant were always neglected. Recently, we analyzed the constituents of ginseng berry, leaf and discovered that ginseng berry, leaf extracts and its total ginsenosides have the ability to reduce hyperglycemia and body weight and increase the peripheral glucose utilization in obese or diabetic ob/ob or db/db mice. Our data suggest that all parts of ginseng plant, including root, berry, leaf and stem exhibit potent anti-hyperglycemic and anti-obese effects and may provide an opportunity to develop a novel class of anti-diabetic agents.