• 제목/요약/키워드: plant seeds

검색결과 1,851건 처리시간 0.037초

In Vitro Multiple Regeneration from Cotyledons and Hypocotyls of Impatiens

  • Shin, Dong-Young
    • Plant Resources
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    • 제2권1호
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    • pp.10-15
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    • 1999
  • In vitro shoot regeneration from cotyledon and hypocotyl explants derived from germinating mature Impatiens seeds. The induction of organogenetic tissue was also influenced by the cotyledon and hypocotyl. Multiple shoot induction was higher in hypocotyl than in the cotyledon explant with Thidiazuron and a NAA medium.

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Comparative Study of Processed (Shodhit) and Unprocessed Seeds of 'Gunja'-Abrus precatorius L.

  • Gautam, D.N. Singh;Singh, P.N.;Mehrotra, Shanta
    • Natural Product Sciences
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    • 제5권3호
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    • pp.127-133
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    • 1999
  • 'Gunja' is attributable to the plant Abrus precatorius L. (Leguminosae). Three forms with red, brown and another with white seeds are known to occur in this species and are employed for different therapeutic uses viz. as purgative, emetic, aphrodisiac, tonic and also as an abortifacient. According to some Ayurvedic literature the seeds are poisonous and should be given to the patients after proper processing ('Shodhan'). A comparative study of various phytochemical parameters, namely, percentage of successive extractives, total proteins, tannins, total ash and acid insoluble ash of these three forms of the processed (with cow's milk and Kanji) and unprocessed seeds was done. TLC and densitometric scanning of successive extractives was also carried out to serve as markers for processed and unprocessed seeds. The percentage of proteins, tannins, alcohol and water soluble extractives decreased in the processed material. Besides, their acute toxicity, CNS activity were also studied in albino mice and it was found that white seeds are more toxic as compared to the red and brown. The toxic effect was reduced with the processing. Further, the 'Kanji' processed seeds are less toxic than the milk processed one.

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온도 및 지베렐린 처리가 말오줌나무 종자의 배발달 및 발아에 미치는 영향 (Effects of Temperature and Gibberellin Treatment on Embryo Development and Germination of Sambucus racemosa subsp. pendula Seeds)

  • 김현진;이기철;서강욱
    • 한국산림과학회지
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    • 제102권2호
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    • pp.204-209
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    • 2013
  • 본 연구는 우리나라 특산 수종으로 종자 발아가 어려운 말오줌나무 종자의 휴면유형을 구명하기 위해 수행되었다. 말오줌나무 종자를 $25/15^{\circ}C$$5^{\circ}C$에서 습윤상태로 각각 0, 6, 12주 처리한 후, 15/6, 20/10, $25/15^{\circ}C$와 30/$20^{\circ}C$에서 14시간 광조건으로 발아실험을 진행하였다. $GA_3$가 종자에 미치는 영향을 알아보기 위해 0, 100, 500, 1000 ppm 을 처리하여 각각 $25/15^{\circ}C$$5^{\circ}C$에서 발아시켰다. 또한 $25/15^{\circ}C$$5^{\circ}C$에서 종자의 배발달 변화를 관찰하였다. 말오줌나무 종자는 $15/6^{\circ}C$에서 12주 저온 층적처리($5^{\circ}C$)가 33.4%, $20/10^{\circ}C$에서는 6주 고온 층적처리($25/15^{\circ}C$) + 12주 저온 층적처리가 25.4%로 발아하였다. $25/15^{\circ}C$에서 말오줌나무 종자는 12주 고온 층적처리 + 6주 저온 층적처리가 26.0%, 30/$20^{\circ}C$에서 12주 고온 층적처리+ 12주 저온 층적처리에서 28.2% 발아하였다. 반면 고온 층적처리 0, 6 또는 12주 처리에서는 모든 온도조건에서 발아하지 않았다. 선형의 배는 $25/15^{\circ}C$$5^{\circ}C$에서 배양 약 60일부터 신장되기 시작하였으며, 배양 69일째 배의 길이는 1.4 mm에서 각각 약 1.50, 1.62 mm로 증가하여, $25/15^{\circ}C$에서보다 $5^{\circ}C$에서 더욱 촉진되었다. $GA_3$는 말오줌나무 종자의 휴면을 효과적으로 타파하였다. $25/15^{\circ}C$에서 $GA_3$ 500 ppm 처리구가 40.0%의 발아율을 나타냈으며, $5^{\circ}C$에서는 $GA_3$ 100 ppm에서 62.7% 발아하였다. 이상의 결과로 말오줌나무 종자는 nondeep complex morphophysiological dormancy(MPD)와 intermediate complex MPD의 휴면유형을 모두 가지고 있다고 판단된다.

Estimation of Cardinal Temperatures for Germination of Seeds from the Common Ice Plant Using Bilinear, Parabolic, and Beta Distribution Models

  • Cha, Mi-Kyung;Park, Kyoung Sub;Cho, Young-Yeol
    • 원예과학기술지
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    • 제34권2호
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    • pp.236-241
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    • 2016
  • The common ice plant (Mesembryanthemum crystallinum L.) has some medicinal uses and recommended plant in closed-type plant factory. The objective of this study was to estimate the cardinal temperatures for seed germination of the common ice plant using bilinear, parabolic, and beta distribution models. Seeds of the common ice plant were germinated in the dark in a growth chamber at four constant temperatures: 16, 20, 24, and $28^{\circ}C$. For this, four replicates of 100 seeds were placed on two layers of filter paper in a 9-cm petri dish and radicle emergence of 0.1 mm was scored as germination. The times to 50% germination were 4.3, 2.5, 2.0, and 1.8 days at 16, 20, 24, and $28^{\circ}C$, respectively, indicating that the germination of this warm-weather crop increased with temperature. Next, the time course of germination was modeled using a logistic function. For the selection of an accurate model, seeds were germinated in the dark at constant temperatures of 6, 12, 32, and $36^{\circ}C$. Germination started earlier and increased rapidly at temperatures above $20^{\circ}C$. The minimum, optimal, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate, as a function of the temperature gradient. The different functions estimated differing minimum, optimal and maximum temperatures, with 5.7, 27.7, and $36.5^{\circ}C$, respectively for the bilinear function, 13.4, 25.0, and $36.6^{\circ}C$, respectively, for the parabolic function and 7.8, 25.9, and $36.0^{\circ}C$, respectively, for the beta distribution function. The models estimated that the inverse of time to 50% germination rate was 0 at 6 and $36^{\circ}C$. The observed final germination rates at 12 and $32^{\circ}C$ were 62 and 97%, respectively. Our data show that a beta distribution function provides a useful model for estimating the cardinal temperatures for germination of seed from the common ice plant.

고추와 토마토 종자에서 종자전염 세균 및 바이러스의 동시 검출을 위한 One-step Multiplex RT-PCR 방법 (One-step Multiplex RT-PCR Method for Simultaneous Detection of Seed Transmissible Bacteria and Viruses in Pepper and Tomato Seeds)

  • 정규식;소은희
    • 식물병연구
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    • 제17권1호
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    • pp.44-51
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    • 2011
  • 우리나라에서 주로 재배되는 가지과작물의 종자 전염 병원균 중에서 세균성 병원균 Xanthomonns campestris pv. vesicatoria(Xcv), Clavibacter michiganensis subsp. michiganensis (Cmm), Erwinia carotovora subsp. carotovora (Ecc)와 바이러스 병원균 Pepper mild mottle virus (PMMoV) and Tobacco mild green mosaic virus (TMGMV)를 종자로부터 동시검출하기 위한 One-step Multiplex RT-PCR을 개발하였다. 각각의 병원균을 특이적으로 증폭시키는 병원균 검출용 프라이머 15세트를 primer-blast 프로그램을 이용하여 제작하였고 각각의 병원균을 특이적으로 검출할 수 있는 5세트의 프라이머 세트(Cmm-F/R, Ecc-F/R, Xcv-F/R, PMMoV-F/R, TMGMVF/R)를 선발하였다. 이들 프라이머 세트는 프라이머간의 또는 병원균 cDNA간의 간섭없이 타겟 병원균만을 검출하였다. 가지과 작물 중에서 유통 중인 고추종자 20품종과 토마토종자 10품종에 대한 종자 감염 병원균 검출을 위한 PCR을 수행한 결과, 2개 품종의 토마토종자에서 Ecc가 검출되었고 4개 품종의 고추종자에서도 Ecc가 검출되었다. 특히 고추 종자에선 Ecc가 검출된 4개 품종 외에 1개 품종에서 PMMoV, TMGMV가 동시 검출되었다. 이로 인해 개발된 One-step multiplex RT-PCR은 서로 간섭 현상 없이 병원균을 효율적으로 검출할 수 있음을 보여 주었다.

Role of Red Light, Temperature, Stratification and Nitrogen in Breaking Seed Dormancy of Chenopodium album L.

  • Tang, Dong-Sheng;Hamayun, Muhammad;Ko, Young-Moon;Zhang, Yi-Ping;Kang, Sang-Mo;Lee, In-Jung
    • Journal of Crop Science and Biotechnology
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    • 제11권3호
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    • pp.199-204
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    • 2008
  • Seed dormancy behavior of weed seeds is a critical determinant of their survival rates in a given cropping system as it helps the weeds to evade herbicides and other weeding practices. We investigated the effects of red light, alternating temperature, stratification duration and different doses of nitrogen containing compounds alone or in combination with red light on breaking seed dormancy of Chenopodium album L. The application of red light(80 ${\mu}mols^{-1}m^{-2}$) significantly increased seed germination of C. album in all treatments. Germination rates of 12 h incubated seeds were highest under 20 min of red light irradiation than 1 min, 5 min and 10 min treated seeds. Germination rate was significantly higher at alternating temperatures of $25^{\circ}C\;and\;5^{\circ}C$ for 12 h each with an irradiation of red light(80 ${\mu}mols^{-1}m^{-2}$) for 10 min than other treatments. Stratification period of 15 days significantly stimulated germination percentage of seeds incubated in dark, although 5 days of stratification along with red light application for 10 minutes exhibit similar effects on seeds. Seed germination was also enhanced by nitrogen containing compounds like $NaNO_2,\;KNO_3,\;NH_4Cl\;and\;NH_4NO_3$. We observed that seed germination increased significantly with 25 mM $KNO_3$ and 10 mM $NH_4NO_3$ in dark condition, while $NaNO_2$ and $NH_4Cl$ enhanced seed germination under red light irradiation. It was concluded that red light alone or synergized with alternating temperatures, stratification and nitrogen compounds, especially nitrite and ammonium enhanced seed germination of C. album. Thus, the red light can play a vital role in present and future weed management strategies.

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검역잡초종자 발아력 제거를 위한 수입농산물 열처리 효과 (Heat-treatment Effects of Agricultural Imports for Devitalization of Quarantine Weed Seeds)

  • 문광옥;오진보;경은선;이용호;홍선희;강병화
    • Weed & Turfgrass Science
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    • 제2권2호
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    • pp.170-175
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    • 2013
  • 우리나라는 열처리방법을 포함한 식물검역규정에 의하여 외래잡초의 유입방지를 위한 잡초검역을 실시하고 있다. 현행 열처리기준의 적절성을 확인하고 검역잡초의 발아력을 완전히 제거하기 위한 열처리조건을 구명하여 식물검역에 활용하기 위하여 검역잡초 9종과 검역잡초와 유사한 잡초 1종의 종자를 재료로 하여 건열처리와 상대습도 40%의 습열처리에 의한 사멸조건을 연구하였다. 다양한 온도 및 시간별 건열처리 결과 $95^{\circ}C$에서 48시간, $100^{\circ}C$에서 36시간, $110^{\circ}C$에서 24시간, $121^{\circ}C$에서 4시간, $130^{\circ}C$에서 30분 처리 후 모든 종자가 사멸되었고, 상대습도 40%에서의 습열처리 결과 $85^{\circ}C$에서 36시간, $90^{\circ}C$에서 16시간 습열처리 후 모든 종자가 사멸하였다. 상기의 결과는 현행 열처리기준이 검역잡초 몇 종에는 적용하기에 적합하지 않음을 보여주며, 검역잡초종자의 발아력을 제거할 수 있는 이 열처리조건은 수입농산물을 통한 검역잡초의 유입을 방지하기 위한 검역의 한 방법으로 적용할 수 있을 것이다. Picris echioides와 같이 강한 내열성이 있는 종과 새삼속(Cuscuta spp.)과 같이 열에 민감한 종을 위한 별도의 열처리조건이 필요하며 이는 다른 내열성의 검역잡초 종자를 사멸시키는 검역업무의 효율성을 향상시킬 수 있을 것으로 사료된다.

Characteristics of Seed and Plant Growth in Local Collections of Agastache rugosa

  • Ok, Hyun-Chung;Chae, Young-Am
    • 한국작물학회지
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    • 제43권4호
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    • pp.269-272
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    • 1998
  • Characteristics of seed and plant growth of the nine local collections of the medicinal plant, Agastache rugosa were evaluated. Seed size of the collection from Garyungsan was larger than the others. Seeds of Damyang collection were the smallest in size. Seeds of the collections were mostly oval shaped. There were no differences between brown and black seed coat color groups in seed width, ratio of seed length to seed width and 1,000 seed weight. Stem lengths of Garyungsan and Bongpyung collections were 99.9 and 95.6 cm, respectively, and were longer than Damyang and Jindo collections. Garyungsan collection had long leaf shape. Leaf size was the smallest for Damyang collection, but was the largest for Bongpyung collection. Inflorescence length of Mokpo collection (15.6 cm) was longer than the average over all collections (12.3 cm). Damyang collection was the shortest (10.1 cm) in inflorescence. Top dry weight of each collection from Soonchun, Bongpyung, Mokpo, Jindo, and Gurye was greater than the average over all collections (20.38 g/plant). Jinju and Damyang collections had less top dry matter than others. There was no difference in the growth traits examined between brown and black seed coat groups. The brown seed group was greater in leaf weight than the black seed group. However, these two groups were not different in stem and inflorescence weight. Collections of A. rugosa from Bongpyung, Soonchun, and Mokpo were promising for commercial cultivation because of their great top dry weight, especially in leaf and inflorescence.

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Interpretation of Relationship Between Sesame Yield and It's components under Early Sowing Cropping Condition

  • Shim Kang-Bo;Kang Churl-Whan;Seong Jae-Duck;Hwang Chung-Dong;Suh Duck-Yong
    • 한국작물학회지
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    • 제51권4호
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    • pp.269-273
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    • 2006
  • Multiple linear regression analysis was conducted to interpretate the relationship between sesame grain yield and its components under early sowing cropping condition. The t test showed that stem length, number of capsules per plant, 1000 seeds weight and seed weight per plant gave significant contribution to sesame grain yield, therefore those variables were assumed to mostly influenced components to grain yield of sesame. In the stepwise regression analysis, the predicted equation for sesame grain yield per square meter (Y) was Y = -7.900 + 0.150X1 + 0.461X5 + 15.553X6 + 8.543X7. Meanwhile, F value showed that stem length, number of capsules per plant and seed weight per plant gave significant contribution to sesame grain yield, while 1000 seeds weight did not significantly show. Based on the results, it is reasonable to assume that high yield. potential of sesame under early sowing cropping condition would be obtained by selecting breeding lines with long stem length, number of capsules per plant, and seed weight per plant, which was different result at the late sowing cropping condition in which days to flowering and maturity were assumed to be more affected factors to the sesame grain yield.