• 생약학회지
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• 제47권1호
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• pp.79-83
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• 2016

This study developed an HPLC analysis method for the determination of isoegomaketone (IK) and perillaketone (PK) in Perilla frutescens (L.) Britton leaves. P. frutescens ethanol extract was optimized through an HPLC analysis using a C18 column ($250{\times}4.6mml$, D, $S-5{\mu}m$, 12 nm) with gradient elution of water and acetonitrile as the mobile phase at a flow rate of 1 mL/min and a UV detection wavelength of 254 nm. The results of this method showed linearity in the calibration curve at a coefficient of correlation ($R^2$) of IK 0.9995, PK 0.9998. The limits of detection (LOD) for IK and PK were $0.234{\mu}g/mL$ and $0.952{\mu}g/mL$. The limits of quantification (LOQ) for IK and PK were $0.017{\mu}g/mL$ and $0.043{\mu}g/mL$. The inter-day precision RSDs of IK and PK in the P. frutescens were 1.25 to 2.69% and 0.36 to 1.10%, respectively, and the intra-day precision RSDs of IK and PK were 0.96 to 2.51% and 0.90 to 1.93%, respectively. The accuracies of IK and PK were 96.31 to 97.92% and 101.26 to 105.14%. In conclusion, this method was applied successfully to the detection of IK and PK in P. frutescens.

• 미생물학회지
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• 제28권2호
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• pp.109-113
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• 1990

Mutator 기능을 가지는 플라스미드 pKM101과 이의 돌연변이체 pSL4를 DNA 수복능력이 다른 Esherichia coli B/r(phr, recA, uvrA, uvrB)균주들에 도입하여 돌연변이원인 UV와 MNNG에 대한 보호효과와 mutagenecity를 조사형ㅅ다. pKM101과 pLS4의 mutator 기능과 보호효과는 repair 기능에 따라 다르나 전반적으로 두 플라스미드의 UV와 MNNG에 대한 저향성과 돌연변이율을 증가시켰고 pLS4는 pKM101보다 그효과가 높았다. 이러한 pLS4와 pKM101의 기능적 차이는 이들 플라스미드의 mutator 유전자상에 일어난 변이에 의한 것이라고 생각되었다.