• Journal of the Korea Furniture Society
• /
• v.17 no.3
• /
• pp.17-21
• /
• 2006

An investigation was done to know the warty layer structural difference in bordered pit of longitudinal tracheid in Pinus densiflora Sieb. et Zucc., Pinus koraiensis Sieb. et Zucc., Pinus rigida Mill. and Larix kaempferi Carr. grown in Korea. Among the four wood species, warty layer was found in bordered pit of longitudinal tracheid of Pinus densiflora Sieb. et Zucc. and Pinus rigida Mill. The mean height and horizontal diameter of warty layer was found highest in Pinus densiflora Sieb. et Zucc. The height of warty layer was found the highest in juvenile wood of Pinus densiflora Sieb. et Zucc. and it gradually decreased towards the matured wood.

• Journal of Korean Society of Forest Science
• /
• v.97 no.1
• /
• pp.88-94
• /
• 2008

The aim of the study was to assess the cosmeceutical activity of Pinus rigida Mill. and it is possible that can be used as a cosmetic ingredient for application of cosmetic industries. The concentration of total phenolic compound of P. rigida water soluble fraction and P. rigida ethyl acetate soluble fraction showed 47 mg/L and 601 mg/L respectively. In the result of DPPH scavenging radical activity, P. rigida ethyl acetate soluble fraction showed 86% and it was similar to BHA effect at 10 ppm concentration. Xanthine oxidase inhibition of P. rigida water soluble fraction and P. rigida ethyl acetate soluble fraction were 76.3% and 80.5% at 500 ppm, respectively. In the result of tyrosinase inhibition effect related to skin-whitening, P. rigida water and ethyl acetate soluble fraction showed 42%, 10.9% at a 1,000 ppm. In these results, P. rigida has a great potential as a cosmeceutical ingredient with a natural anti-oxidant source.

• The Korean Journal of Ecology
• /
• v.14 no.3
• /
• pp.273-303
• /
• 1991

A phytosociological study of forest vegetation was carried out in mt. komdan in the period from October 7,1985 to October 15 1989. The forst vegetation could be classified into 7 communities ; quercus monbolica,pinus densiflora, quercus dentata, pinus koraiensis, larix leptoletsis, pinus rigida,castanea creaneta. The principal forest community in mt. komdan-the quercus mongulica community- was distributed mainly at the altitude of 400~600m and the importance value was 187.1. The pinus desiflora community was 137.9. since pinus koraiensis, larix laptolepsis,pinus rigida, castanea creanata, etc. werw planted in this region, It seems that it was frequently disturbed by human interference,especilly, the destruction of the vegetation in the recreation ground of paldang, the regions around the village of hasangok-ri and the main routes for mountain climbing appeared severely.

• Journal of the Korea Furniture Society
• /
• v.18 no.2
• /
• pp.138-142
• /
• 2007

Optical microscope was used to observe the difference of safranine penetration in Pinus densiflora, Pinus rigida, Pinus koraiensis and Larix kaempferi grown in Korea. It was found that Pinus koraiensis contained the highest number of ray parenchyma and ray tracheids. In longitudinal direction, latewood penetration was found higher than that of earlywood. The number of resin canals was found highest in Pinus koraiensis and lowest in Pinus rigida. The resin canal conducted safranine higher than longitudinal tracheids. In longitudinal direction, safranine diffused from longitudinal tracheid to ray parenchyma through the cross-field pits and from the longitudinal resin canal to ray parenchyma or longitudinal tracheid. Safranine diffused from longitudinal tracheid to its neighboring tracheid through bordered pit or ray parenchyma through the cross-field pits.

• Applied Microscopy
• /
• v.42 no.3
• /
• pp.147-150
• /
• 2012

A recently introduced helium ion microscopy (HIM) was employed to observe uncoated pine leaf specimens. Adult leaves were collected from the seedlings of Pinus densiflora and P. rigida, air-dried at room temperature, and observed by HIM without metal coating. Ovoid or round stomata and distinct Florin rings could be discerned. The epicuticular waxes were present in the epistomatal chambers and Florin rings of stomata on the leaf surface. The epicuticular waxes were mostly straight, cylindrical, and ca. 1 ${\mu}m$ in length. The epistomatal chambers of P. rigida were filled with the epicuticular waxes, whereas those of P. densiflora were not filled with the epicuticular waxes. Based on their micromorphology, the epicuticular wax structures of the pine species were identified as tubules. These results suggest that the HIM could be used for the investigation of the plant stomata and epicuticular waxes of uncoated plant leaves. Due to the smaller ion probe and interaction volume, the HIM has advantages over conventional field emission scanning electron microscopy in terms of image resolution and charge neutralization.

• The Korean Journal of Ecology
• /
• v.20 no.4
• /
• pp.245-250
• /
• 1997

The seeds and callus induced from the root of Glycine max were used as test materials. When the seed was treated with the different concentrations of Pinus rigida extract, there was a more striking inhibition of seedling growth than of seed germination. The callus of the control group was in good condition, but when treated with 5% extract there was generalized browning and cell division was decreased in the upper part of the callus. There was no difference in the fresh and dry weights in 2% extract treatment but there was dramatic repression at concentrations higher than 5%. The band activity of peroxidase isozyme in treated callus was elevated, while that of esterase was inhibited.

• Mycobiology
• /
• v.36 no.4
• /
• pp.222-227
• /
• 2008

Upon studying the diversity of fungal endophytes associated with pine trees in Korea, many species of Penicillium were encountered. In this study, we report two species of Penicillium isolated from the needles of Pinus rigida. Based on ID region analysis, cultural and morphological characteristics, the two species were identified as Penicillium fellutanum and P. toxicarium, both of which are new to Korea.

• The Korean Journal of Mycology
• /
• v.46 no.4
• /
• pp.437-446
• /
• 2018

We investigated the biodiversity of endophytic fungi in Pinus densiflora and Junipreus rigida from Mt. Baekryeonsan and Mt. Johangsan in Korea. In total, 59 isolates were discovered from 26 host plants and identified using sequences from the rDNA internal transcribed spacer (ITS) region. Twenty taxa of endophytic fungi were identified in this study; 62% of isolates belonged to Leotiomycetes, 34% belonged to Sordariomyetes, and 4% belonged to Agaricomycetes. Results confirmed three previously unreported endophytic fungi in Korea: Phanerochaete concrescens, Creosphaeria sassafras, and Scopuloides rimosa. Community analysis showed that the fungal diversity patterns of the samples from Mt Baekryeonsan and Mt. Johangsan are similar to those from previous studies and varied depending upon host and locality.

• Journal of Korean Society of Forest Science
• /
• v.65 no.1
• /
• pp.1-11
• /
• 1984

Susceptible trees of Pinus densiflora and resistant trees of Pinus rigida following pine gall midge (Tnecodiplosis japonensis Uchida et Inouye) attack were seasonally compared to examine the variation in needle growth and photosynthetic ability, respiration rate, chlorophyll contents, carotenoid and anthocyanin contents. Also, carotenoid and anthocyanin contents of larvae both from soil and from galled tissue were compared during March and September, respectively. The plantation damaged severely by this insect consisted mostly of 10-to 15-year old P. rigida and P. densiflora. The results obtained in this study were as follows: 1) The length of the infested needles of P. densiflora decreased by 48.1 percent compared with the normal needles, while that of P. rigida did 37.4 percent. 2) All of P. densiflora and P. rigida showed higher photosynthetic ability in normal needles than in infested needles. The maximum photosynthetic ability of P. densiflora was shown in mid-August, while that of P. rigida in mid-October. In contrast to that, respiration rate of infested needles was higher than that of normal needles in both species. The respiration rate of P. rigida was higher than that of P. densiflora. 3) P. rigida had higher total chlorophyll contents than P. densiflora. The total carotenoid contents tents in infested needles were higher than those in normal needles of both species. 4) Total carotenoid contents were generally higher in P. rigida than in P. densiflora during the growing season. The total carotenoid content (0.094mg/g) in larvae from soil was similar to that (0.092mg/g) in larvae from galled tissues. 5) Infested needles of both species showed higher anthocyanin contents than normal needles. Higher anthocyanin contents in galled needles were due primarily to its active formation stimulated by larval attack. Thus, reddish-brown coloration occurred only in galled needles of P. densiflora.

• Journal of Korean Society of Forest Science
• /
• v.94 no.6
• /
• pp.402-409
• /
• 2005

This study was undertaken to compare and estimate the severity of pitch canker of individual trees of Pinus rigida and Pinus rigida ${\times}$ P. taeda in two seed orchards in Jeju island, in which the orchards have been damaged by the pitch canker for seven years. Wind-pollinated two-year-old seedlings of P. rigida and P. rigida ${\times}$ P. taeda, in which the seedlings of P. rigida ${\times}$ P. taeda were from seeds of phenotypically selected, uninfected(but untested) trees, were inoculated with the pathogenic fungus, Fusarium circinatum, isolated from P. rigida and P. thunbergii. The virulence of the isolates was also identified. Statistically significant difference was found in 'stem cankers'(SC; ${\chi}^2=7.76$, P=0.05) among 4 plantations of P. rigida ${\times}$ P. taeda of two seed orchards. P. rigida was higher in 'top kill' (TK) and 'branch tip symptoms' (BT) than those of P. rigida ${\times}$ P. taeda. In artificial inoculation tests, mortality of the seedlings from the resistant candidates was 14% higher than that of the seedlings from the susceptible candidates. This result may becaused by unknown pollen trees and/or candidate tree selection based only on phenotype. Two of five fungal isolates, C-6-L(9) and C-6-L(19), showed significantly higher mortality (68% and 60%, respectively) than others, suggesting that these isolates can be used as virulent isolates for a mass artificial inoculation. Resistance candidate seedlings that were selected from this study can be utilized as useful materials for fundamental studies of genetics and biochemistry to breed resistance varieties to pitch canker.