• Journal of the Korean Applied Science and Technology
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• v.40 no.1
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• pp.103-112
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• 2023

This study selected 9 species of domestic native plants (Baechohyang, Red Pine, Turmeric, Ginger, San parsley seed, Bulsugam, Hwangchil, Tangja, Valerian) that have been proven effective for depression, and based on the analysis results of each fragrance component, 9 species were selected. Antioxidant (DPPH, ABTS), cytotoxicity (MTS), and anti-inflammatory (Nitric oxide) experiments were performed by combining essential oils. As a result of the analysis of fragrance components, DL-Limonene (38.44%), g-Terpinene (8.9%), Estragole (5.18%), and a-Pinene (1.73%), which were previously studied in combination oil, were identified. DPPH radical scavenging ability, which is an antioxidant activity, showed 75.4%, ABTS radical scavenging ability was 74.04%, and NO production inhibition was 33.14% at a concentration of 5uL/ml with no cytotoxicity confirmed. Through this, the effect of domestic blending essential oils on the improvement or prevention of depression is verified, and scientific efficacy and ingredient studies are conducted in a mutually cooperative manner to seek solutions to depression and provide basic data to confirm whether or not depressive symptoms are improved. want to do.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.109-109
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• 2020

꽃차(Flower tea)는 최근 다양한 제품이 개발되고 소비가 확대되는 등 그 산업적 가치가 증대하고 있다. 꽃차의 향기 특성은 우렸을 때 나타나는 색깔 및 인체에서의 생리활성과 더불어 주요한 꽃차 품질결정 요소이다. 본 연구는 꽃차로의 이용이 활발한 국화과 식물 중 구절초(Dendranthema zawadskii var. latilobum), 국화(Chrysanthemum morifolium), 노랑색 및 주황색 마리골드(Tagetes erecta 'Yellow' and 'Orange'), 그리고 캐모마일(Matricaria chamomilla)의 향기 성분특성을 구명하기 위하여 제조된 꽃차를 headspace-solidphase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS)를 이용하여 분리, 동정하였다. 국화과 꽃차로부터 총 117종의 휘발성 성분이 확인되었는데, 각 꽃차 종류별 동정된 휘발성 성분의 개수, 주요 3개 휘발성 성분과 전체 향기성분 peak중 이들이 차지하는 구성비율(%)은 다음과 같았다: 구절초 64종, camphor (31%), α-pinene(14%), camphene(14%); 국화 60종, camphor(15%), chrysantheny acetate(13%), eucalyptol (11%); 마리골드 '옐로우' 53종, 2,4-heptadienal(26%), trans-isocarveol(21%), cis-β-Copaene(18%); 마리골드 '오렌지' 61종, β-caryophyllene(16%), β-ocimene epoxide(12%), β-ocimene(12%); 캐모마일 50종, β-farnesene(63%), nonane(9%), spathulenol(5%). 국화과 꽃차 5종 모두에서 공통적으로 검출된 성분은 β-caryophyllene, α-pinene, β-farnesene 등 10종이었으며 마리골리 '옐로'는 '오렌지'와 주요 향기성분의 조성에서 뚜렷한 차이를 나타내었다. 비록 그 함량은 낮았으나 구절초, 국화, 마리골드 '오렌지', 그리고 캐모마일은 각각 10종, 12종, 3종 및 13종이었다. 마리골드 '엘로'의 경우 검출된 모든 향기성분은 마리골드 '오렌지'나 다른 국화과 식물의 꽃차에서도 검출된 바, 향기 성분이 다양성이 다소 낮게 나타났다.

• Atmosphere
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• v.34 no.2
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• pp.187-202
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• 2024

We analyzed the observed characteristics of monoterpene and developed an empirical formula for monoterpene concentration in the pine-dominated mixed forest of the National Center for Forest Therapy. Monoterpene was measured at 0800, 1200 and 1700 LST once a month using sorbent tube sampling coupled with thermal desorption gas chromatography and mass spectrometry. Monoterpene concentration is low in winter and shows a maximum in June and July. The major components of monoterpene are alpha-pinene, camphene and beta-pinene. During the warm period from May to November, monoterpene concentration is higher at 0800 and 1700 LST than at 1200 LST. The empirical formula takes into account the vegetation variables, temperature-controlled emission, oxidation processes and dilution by wind. The vegetation variable accounts for the difference in observed monoterpene concentration between two sites. The observed monoterpene concentration normalized by the vegetation variable increases exponentially with air temperature. The oxidation process explains the lower monoterpene concentration at 1200 LST than at 0800 and 1700 LST during the warm period. The monoterpene estimates using the empirical formula shows a correlation of 0.52 with the observation for the development period (2018~2020), while it shows a correlation of 0.72 for the validation year (2021). Such higher correlation for the validation year than for the development period is due to the fact that variability of monoterpene concentration is better explained by air temperature in 2021 than in the development period. However, the developed formula underestimates the monoterpene concentration in May and June, showing the limitation in accurately capturing the monthly variation of monoterpene.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.15-22
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• 2023

The aim of the present study was to evaluate the chemical composition and antioxidative activity of rhizome essential oil of Kaempferia parviflora Wall. ex Baker. The essential oil extracted by hydrodistillation was chemically profiled by GC/MS analysis. The antioxidative activity was determined and evaluated spectroscopically by the 2,2-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assays. According to the results, the major essential oil components were camphene (18.03%), β-pinene (14.25%), a-pinene (12.38%), endo-borneol (10.23%), β-copaene (8.38%), and linalool (8.20%). K. parviflora rhizome oil possessed antioxidant potential, exhibiting DPPH and ABTS radical scavenging activities as high as 80.90 and 94.04%, respectively, at a concentration of 10 mg/mL. The corresponding IC₅₀ values were 0.451±0.051 and 0.527±0.022 mg/mL, respectively (IC₅₀ values for ascorbic acid, as the standard, were 0.209±0.016 and 0.245±0.022 mg/mL, respectively). The mycelium of F. oxysporum was distorted and collapsed when treated with 0.5 mg/mL of the EO of K. parviflora rhizome for 3 days treatment, which may provide an important information for exploring the metabolism of the fungicide K. parviflora rhizome and its derived compounds against F. oxysporum. This study provides the chemical properties of the essential oil of K. parviflora rhizome grown in Vietnam and their potential antioxidant and antifungal activities.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.983-993
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• 2022

The purpose of this study is to provide information on a forest healing environment using the analysis of nature volatile organic compounds (NVOCs) and thermal comfort in Chungnam National University Experimental Forest, with the aim of using the Experimental Forest as a healing environment for health promotion. We analyzed NVOCs and thermal comfort of Chungnam National University Experimental Forest measured on September 12^th, 2021. As a result of the NVOC analysis, a total of seven substances were detected, mainly including alpha pinene and beta pinene. The detection amount for each time period was highest at the time of sunset. The thermal comfort was analyzed by time-dependent changes and changes according to clothing and exercise amount. The results showed that the predicted mean vote of the experimental forest is within the range of 'slightly cool' and 'slightly warm' sensation, and thus a comfortable thermal environment could be controlled by the amount of clothing and activity. Based on the analysis, this study provides information on the healing environment of the experimental forest at Chungnam National University. It also indicates that the forest can be used as a health promotion and healing environment with thermal comfort by composing a physical activity program of appropriate intensity for each time period.

• Journal of the Korean Applied Science and Technology
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• v.41 no.3
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• pp.733-744
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• 2024

This study extracted essential oil from the native Korean plant Valeriana fauriei and performed fragrance component analysis, antioxidant (DPPH, ABTS), cell viability (MTS), and anti-inflammatory (Nitric oxide) experiments based on the analysis results. The fragrance component analysis revealed that the major effective component of Valeriana fauriei, bornyl acetate, was present at a high content of 47.88%, compared to other regions. Additionally, patchouli alcohol (18.9%), camphene (11.37%), α-Pinene (5.44%), and D-limonene (1.11%) were identified. The antioxidant activity showed that the DPPH radical scavenging activity was 73.62% at 250 µl/ml, and the ABTS radical scavenging activity was 82.17% at 250 µl/ml. At a concentration of 5 µl/ml, which did not exhibit cytotoxicity, the NO production inhibition rate decreased by 62.02% compared to the control group. Through these findings, the potential for the application of Valeriana fauriei essential oil in functional products has been scientifically validated, contributing to research utilizing Valeriana fauriei essential oil.

• Analytical Science and Technology
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• v.28 no.3
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• pp.246-254
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• 2015

From 2013 to 2014, volatile organic compounds (VOCs) were analyzed to determine biogenic volatile organic compounds (BVOCs) and anthropogenic volatile organic compounds (AVOCs) at eight sites in Mt. Mudeung and one site in downtown, by using a GC/Mass-ATD (automatic thermal desorber). The concentration of terpene noted as biogenic volatile organic compounds at Pungamjeong (PA), in a forest of Chamaecyparis obtusa, was 821 pptv, which was the highest among the eight sites. This value was followed by Wonhyogyegok (WH: 785 pptv), Norritzae (NZ, coniferous forest: 679 pptv), Dongjeokgol (DJ, mixed species forest: 513 pptv), Jangbuljae (JB, Abies koreana: 476 pptv), and Seinbongsamgerri (SS, pine trees: 464 pptv). 11~15 species of terpene was detected in the forest depending on the site. At PA in May, α-pinene showed the highest value, occupied 20% of terpene followed by coumarin, sabinene, phellandrene, myrcene, borneol, eucalyptol, β-pinene, cymene, δ-limonene, γ-terpinene, camphor, camphene, and mentol in the order. The mean concentrations of AVOCs were 0.74~2.52 ppbv in the forests and 3.14 ppbv in the downtown area. From May to July, the AVOCs ratios of the downtown to each forest were 1.9~4.0. Among 10 species of AVOCs, the sum of toluene and benzene was 2.34 ppbv and occupied 75%. In June, the ratios of toluene were 44.1% at DJ site and 53.1% at JW site (downtown). The BVOCs showed a positive correlation with the AVOCs at the forest sites (r = 0.328), which was statistically insignificant (p = 0.184).

• Journal of Bio-Environment Control
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• v.24 no.1
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• pp.21-26
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• 2015

This study was conducted to compare the aromatic compounds and the internal quality such as total phenolic contents, DPPH activity, and vitamin C contents in two new forma (red type and dalgom) and gom-chwi (Ligularia fischeri). Total phenolic contents were indicated 137.29mg/100g fresh wt. in gom-chwi, 158.40mg/100g fresh wt. in red type, and 180.82mg/100g fresh wt. in dalgom, red type and dalgom had higher contents than gom-chwi. DPPH activities were appeared similar result in gom-chwi (74.45%) and red type (75.59%), and dalgom (86.26%) had better than others. In vitamin C contents, gom-chwi and red variety had shown very closed results but dalgom had best result (1.81mg/100g fresh wt.). Essential oil contents of red type (0.164% of fresh wt.) and dalgom (0.290% of fresh wt.) had higher contents than gom-chwi (0.060%). Analysis of major components of essential oil by GC/MSD, identified 43 compounds in gom-chwi, 44 compounds in red type, and 31 compounds in dalgom. N-containing compound only detected in dalgom. Comparison of six aromatic compounds for fragrance (a-pinene, ${\beta}$-pinene, ${\beta}$-phellandrene, a-phellandrene, 3-carene, and limonene) was indicated red type had higher contents and dalgom had lower contents than gom-chwi. Dalgom had higher contents of total phenolic, DPPH activity, vitamin C, and essential oil than red type and gom-chwi. This result had shown the dalgom had better characters for new cultivar.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.17 no.1
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• pp.29-38
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• 1991

As the suitable place of the forest bathing, Two places of the needle-leaf trees, which are located in the Oh-Saek mineral spring near place from the Sorak mountain, were selected. And then, Headspace gas trapping apparatus were setted in that two places and the aromatic components of the forest were adsorbed by Tenax-TA column for 24 hours. And Tenax-TA column were analyzed by the GC SE GC-MS. The analyzed components were found to contain up to between 70-80% of pollutants, which are Toluene, Methyl Chloride, Hexane, p-Xylene, Benzene, ... etc. On the other hand, the aromatic components of the forest, which give aromatheraphitical effectness, are as follow: alpha-Pinene, Limonene, 1, 8-Cin-eol, Benzaldehyde, . . . etc.

• The Korean Journal of Ecology
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• v.16 no.2
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• pp.181-190
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• 1993

To elucidate phytotoxic effects on the growth of receptor plant, germination and growth experiment of selected species have been performed with aqueous extracts and volatile substances of leaf and of donor plant, Thuja orientalis. The extracts of T. inversely proportional to the concentration. Gas chromatography method was employed for analysis and indentification of phytotoxic substances from T. orientalis. Forty-two kinds of KDICicals including ${\alpha}-thujone$ were identified from T. orientalis essential oil. Bioassay was performed with 6 KDICical such as ${\alpha}-pinene, \;{\alpha}-terpinene, \;{\gamma}-terpinene, \; {\beta}-myrcene$, and among them bornyl acetate was the strongest growth inhibitor.