• 한국염색가공학회지
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• 제25권4호
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• pp.327-336
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• 2013

The purpose of this study was to evaluate the color image of the natural-dyed silk fabrics. The dye was extracted from pine needle by boiling pine needle with ethanol at $78^{\circ}C$ for 3hours and distilled water at $100^{\circ}C$ for 2hours. The 100% silk fabric was dyed of extract in pH 5 at $90-100^{\circ}C$ for 1 hr. As mordants used were compounds of Al, Sn, Fe, and Cr, color image of pine-needle dyed silk fabrics was classified into 5 factors (pure, gentle, sophisticate, comfortable, pastorale) and the factor pure is most important one of those. Most cheerful image in pure factor was from the fabrics dyed with ethanol extract and then, none and Cr mordanting. Dignified image was from the fabrics dyed with ethanol extract and then, Cu or Fe mordanting. In production, products dyed with ethanol extracts was preferred to those dyed with distilled water extracts. Color image and preference of the silk fabrics dyed with pine needles extracted was affected by extraction solvents and mordants.

• Applied Biological Chemistry
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• 제36권6호
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• pp.469-475
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• 1993

솔잎혹파리(Thecodiplosis japonensis Uchida et Inouye) 방제용으로 소나무의 수간에 주입된 침투성 살충제 phosphamidon(2-chloro-2-diethylcarbamoyl-1-methylvinyl dimethyl phosphate)의 대사를 구명하기 위하여 $[vinyl,\;carbonyl-^{14}C]phosphamidon$을 약 10년생 소나무의 수간에 주사하고 그 솔잎 추출액을 autoradiography한 결과 phosphamidon은 소나무 체내에서 신속히 분해됨을 알 수 있었다. 솔잎의 phosphate buffer 추출액 중에서 phosphamidon은 주대사산물인 ${\alpha}-chloroacetoacetic$ acid diethylamide를 비롯하여 ${\alpha}-chloroacetoacetic$ acid ethylamide, 3-hydroxy-N,N-diethylbutanamide, acetoacetamide, 그리고 trimethyl phosphate 등의 대사산물을 생성하였으며, 이 대사작용은 소나무 체내에서도 유사하리라 추정된다. 또한 주 대사경로는 구조식중 P-O-vinyl 결합의 가수분해와 관련있는 것으로 보인다.

• 복식
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• 제56권2호
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• pp.147-154
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• 2006

In this study 1, the dyeability and sun protection characteristic of the fabrics dyed with pine needles extract was investigated. In the test of color difference, the surface color of all the dyed fabric of both cotton and silk came to light as greenish yellow. In the test of dyeing colorfastness, the colorfastness to sunlight of dyed cotton fabric was below 2nd grade and the one of dyed silk fabric was below 3rd grade. The colorfastness to laundry for the degree of discoloration showed a difference as to mordants, but the colorfastness to laundry for the degree of contamination was excellent as all the dyed fabrics were 4th-5th grade. The colorfastness to perspiration was excellent as the degree of discoloration was more than 3rd grade and the degree of contamination was more than 4th-5th grade. The colorfastness to rubbing showed the excellent result as all the dyed fabrics were 4th-5th grade. In the test of sun protection factor(SPF), the cotton and silk fabrics dyed with $FeSO_4{\cdot}7H_{2}O$ mordant showed very high sun protection characteristic as the SPF was each 36.1 and 42.5. In the test of sun protection rate for UV-A and UV-B, the cotton and silk fabrics dyed with $FeSO_4{\cdot}7H_{2}O$ mordant showed the very high sun protection rate of more than $90\%$.

• Applied Biological Chemistry
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• 제37권4호
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• pp.310-314
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• 1994

솔잎으로부터 프리 라디칼 소거 작용이 있는 물질을 분리하고, 여러 기기 분석 결과에 근거하여 4-hydroxy-5-methyl-3[2H]-furanone으로 동정하였다. 아울러 xylose와 glycine의 Maillard 반응을 통하여 이 물질을 합성하였다. 이 물질은 1,1-diphenyl-2-picrylhydrazyl(DPPH) 프리 라디칼에 대해 소거 작용을 보였으며(100 M의 DPPH의 50%를 환원 시키는데 필요한 농도, 즉 $SC_{50}=26\;{\mu}M$) 이는 구조적으로 유사한 공지의 항산화 물질인 $4-hydroxy-2,5-dimethyl-3[2H]-furanone(SC_{50}=53\;{\mu}M)$과 $3-hydroxy-2-methyl-{\gamma}-pyrone(SC_{50}=4.0\;mM)$에 비해 강력한 것이다. 4-Hydroxy-5-methyl-3[2H]-furanone은 3,4-dihydroxyphenylalanine과 linolenic acid의 자동 산화에 대해서 억제 작용을 나타내었다.

• 한국식품조리과학회지
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• 제27권5호
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• pp.567-575
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• 2011

본 연구에서는 한국 전통차(장미차, 감국차, 솔잎차, 감잎차, 뽕잎차 및 녹차)의 총 폴리페놀 및 플라보노이드 함량, 전자공여능, 아질산염 소거능 및 항균효과에 대하여 비교, 조사하였다. 장미차, 감국차, 솔잎차, 뽕잎차, 감잎차 및 녹차 추출물의 총 폴리페놀 함량은 각각 272.8, 74.6, 153.5, 73.5, 69.5 및 260.8 mg/g이었으며, 총 flavonoid 함량은 각각 75.1, 47.8, 26.8, 40.0, 27.9 및 99.5 mg/g이었다. 장미차, 감국차, 솔잎차, 뽕잎차, 감잎차 및 녹차 추출물(1 mg/mL)에 대한 전자공여능은 각각 96.8, 35.8, 71.3, 28.9, 28.8 및 95.3%이었다. pH 1.2에서의 아질산염 소거능(2 mg/mL)은 녹차(91.3%) > 장미차(49.6%) > 솔잎차(46.0%) > 감국차(15.4%) ${\qeq}$ 뽕잎차(14.2%) ${\qeq}$ 감잎차(13.5%)의 순이었다. SOD 유사활성능(5 mg/mL)은 녹차(18.7%) > 장미차(14.7%) > 뽕잎차(14.0%) > 솔잎차(11.2%) > 국화차(8.4%) ${\qeq}$ 감잎차(7.9%)의 순으로 녹차 추출물이 가장 높았으며, 국화차 및 감잎차 추출물은 거의 없었다. 장미차, 감국차, 솔잎차, 뽕잎차, 감잎차 및 녹차 추출물(5 mg/mL)의 hydroxyl radical 소거능은 각각 55.3, 64.4, 35.3, 51.5, 44.6, 29.5%이었다. 장미차의 경우 Micrococcus luteus, Bacillus cereus, Salmonella enteritidis, E. coli 모두에 대해 항균효과를 보였으며, 특히, Gram 음성균인 Salmonella enteritidis에 대해 강한 항균력을 보였다. 녹차, 솔잎의 경우 Salmonella enteritidis, Bacillus cereus에 대해 항균력을 나타내었다. 그러나 뽕잎, 감잎, 국화차의 경우는 Micrococcus luteus, Bacillus cereus, Salmonella enteritidis, E. coli 모두에 대해 항균효과를 보이지 않았다.

• Nutritional Sciences
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• 제7권2호
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• pp.76-82
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• 2004

Pine needles are known as a traditional medicine and their ingestion has been shown to be beneficial to human beings. Following induction of the neoplastic process in rats by azoxymethane (AOM), we determined the effects of pine needle supplementation on colon carcinogenesis and on antioxidant systems in the blood and liver. Five week old male Fisher 344 rats were injected with AOM (15 mg/kg) once a week for two weeks. After the second injection, 18 rats were randomly assigned into two groups and were fed a casein-based high-fat diet (120 g fat and 1 g cholesterol/kg diet) with or without pine needle powder (10%w/w). After 6 weeks, rats receiving pine needle powder showed a 40% lower incidence of the number of colonic preneoplastic lesions (aberrant crypts) and a 52% lower incidence of aberrant crypt foci (p<0.01). A significantly elevated level of erythrocyte catalase activity was observed in the pine needle supplemented group (17.4$\pm$1.1 vs. 24.5$\pm$1.5, p<0.01). Pine needle supplementation also increased liver glutathione peroxidase activity (7.5$\pm$0.6 vs. 14.6$\pm$0.6, p<0.01). Other antioxidant parameters such as erythrocyte glutathione peroxidase, liver catalase activity, and plasma total antioxidant potential (TRAP), showed no statistical differences between the two groups. Our data demonstrate that pine needle supplementation improves the antioxidant system and suppresses the formation of colonic preneoplastic lesions in AOM-treated rats. This result provides additional insights into the chemo-preventative properties of pine needles.

• 한국식품영양과학회지
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• 제28권5호
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• pp.973-979
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• 1999

Volatile compounds from twigs, needles and sprouts in Pinus densiflora were extracted with n hexane, diethyl ether or ethanol for 24 hours, and the extracted compounds were separated and identified by gas chromatography and mass selective detector. The kinds and amount of volatile compounds extracted from three parts of Pinus densiflora were different in solvent extraction and the extraction by the modified Liken Nickerson apparatus. The contents of volatile compounds of twigs contained more than those of needles and sprouts, and the volatile compounds were extracted more in n hexane than the others. In the extraction with hexane, the main volatile compounds of twigs were 18.5% pinene, 14.5% limonene, 12.7% pinene and 3.2% myrcene. Sprouts were 16.8% limonene, 4.4% pinene, 4.3% pinene and 1.7% myrcene. Needles contained 14.7% pinene, 5.4% pinene, 2.2% limonene and 0.8% myrcene. The highest yield for pine aroma was shown in the extraction from pine twigs with n hexane, and in this extraction the amounts of pinene, pinene and limonene were 742 g, 1108 g and 922 g per gram sample, respectively.

• Nutrition Research and Practice
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• 제5권4호
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• pp.281-287
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• 2011

In this study, we evaluated the antioxidant activity of pine needle extracts prepared with hot water, ethanol, hexane, hot water-hexane (HWH), and hot water-ethanol (HWE), using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical method. The hot water extract possessed superior antioxidant activity than the other extracts. We also compared the antioxidant activity of pine needle extracts through ROS inhibition activity in a cellular system using MC3T3 E-1 cells. The hot water extract exhibited the lowest ROS production. The pattern of HPLC analysis of each extract indicated that the hot water extract contained the highest proanthocyanidin level. The pine needle hot-water extract was then isolated and fractionated with Sephadex LH-20 column chromatography to determine the major contributor to its antioxidant activity. The No.7 and 12 fractions had high antioxidant activities, that is, the highest contents of proanthocyanidins and catechins, respectively. These results indicate that the antioxidant activity of procyanidins from the hot water extract of pine needles is positively related to not only polymeric proanthocyanidins but also to monomeric catechins. Moreover, the antioxidant activity of the pine needle hot water extract was similar to well-known antioxidants, such as vitamin C. This suggests that pine needle proanthocyanidins and catechins might be of interest for use as alternative antioxidants.

• Applied Biological Chemistry
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• 제20권1호
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• pp.81-87
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• 1977

잎혹파리 내충성 및 피해 해송의 1년생 가지, 신초 및 침엽중 monoterpene조성을 GLC로 분석하여 다음과 같은 결과를 얻었다. 1. 착생 방위별 (남, 북) 1년생 가지 및 신초의 monoterpene조성에는 차이가 없었다. 2. 일년생 가지의 주요 moneterpenes는 ${\alpha}-pinene,\;{\beta}-pinene$, myrcene, limonene, ${\beta}-phellandrene$ 및 terpinolene였다. 3. 신초및 침엽중 monoterpene조성은 ${\alpha}-pinene$, camphene, ${\beta}-pinene$, ${\Delta}^3-carene$, myrcene, limonene, ${\beta}-phellandrene$, terpinolene외에 확인되지 않은 고비점 terpenoids로 구성되었다. 4. limonene의 조성은 피해목보다 선발목에 더 많았다. 5. ${\beta}-pinene$의 조성은 선발목보다 피해목에 더 많았다. 6. 선발목과 피해목을 limonene과 ${\beta}-pinene$의 조성의 차이로 고찰하였다.

• The Korean Journal of Ecology
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• 제28권5호
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• pp.321-326
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• 2005

안산 공단지대에 식재되어 있는 잣나무와 리기다소나무의 생장 저하의 실태와 그 원인을 구명하기 위하여 생장 및 생리학적 반응을 중심으로 대조구와 비교 고찰하였다. 피해구에 식재 되어 있는 두 수종의 침엽 내 Mn, F 그리고 Cl의 농도는 대조구에 비하여 유의적으로 높았으며, 2년생 3년생으로 갈수록 급격히 그 농도가 증가하였다. 또한 필수 영양 원소인 P와 클로로필 함량은 피해구가 대조구에 비하여 유의적으로 감소하였으며, 또한 침엽의 연령이 증가함에 따라 급격히 감소하였음을 알 수 있었다. 한편, 피해구에 식재되어 있는 두 수종의 최대 광합성률, 양자수율$(\Phi)$, 카르복실레이션효율(CE)등의 광합성 능은 대조구에 비하여 현저히 감소하였다. 이상과 같이 독성 원소의 축적 및 필수 영양 원소의 부족 등 환경 스트레스에 의한 수목 생리활동의 저하가 수목 생장 저하의 직접적인 원인으로 판단된다.