• Title/Summary/Keyword: phytotoxicity

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Establishment of Bioassay System for Developing New Insecticides I. Effects of Organic Solvents on the Toxicity against Insects, Phytotoxicity and Solubility of Compounds (살충제 개발을 위한 생물검정법의 확립 I. 각종 유기 용매가 곤충의 독성과 약해 및 화합물의 용해성에 미치는 영향)

  • 안용준;조광연
    • Korean journal of applied entomology
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    • v.31 no.2
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    • pp.182-189
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    • 1992
  • The influnces of 9 kinds of solvents on the toxicities against several insect species, phytotoxicity and solubility of compounds were evaluated by means of leaf dipping and spray methods. In case of the spray application, density and vapor pressure seemed to be a contributing factor to lethal toxicity against brown planthopper and diamond-back moth, respectively; the bigger the property of density and the smaller the vapor pressure gave the stronger toxicity. It appeared that the toxicity of solvents was not correlated with anyone of physical properties such as boiling point, dipole moment, dielectric constant, surface tension and viscosity. Spray treatment gave more toxicity to insects than leaf dipping treatment. Although dielectric constant and dipole moment seemed to be contributing factors to phytotoxic damage to rice seedling and bean plants, respectively, no general correlation between phytoxicity and the other physical properties was found. Leaf dipping application caused stronger phytotoxicity than spray application. It is concluded that 5% acetone solution may be most suitable to test chemicals because of its favorable solubility of compounds, lower toxicity to insects, and lower phytotoxicity.

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Mechanism of Phytotoxicity of Dithiopyr in Rice (벼에서 Dithiopyr의 약해발생(藥害發生) 기구(機構))

  • Kang, K.S.;Pyon, J.Y.
    • Korean Journal of Weed Science
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    • v.14 no.2
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    • pp.101-106
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    • 1994
  • Factors affecting phytotoxicity of dithiopyr in rice such as transplanting depth, seedling age, soil texure were examined and mechanism of phytotoxicity in rice was also determined by absorption study of $^{14}C$-dithiopyr in rice seedlings under above conditions. Rice injury was occurred in shallow transplanting depth, young rice seedlings and sandy soil conditions. Higher amount of dithiopyr was absorbed in rice at shallow transplanting depth and sandy soil conditions which may related to phytotoxicity of dithiopyr.

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Assessing the phytotoxicity of cetrimonium bromide in plants using eco-physiological parameters

  • Song, Uhram;Kim, Han Eol
    • Journal of Ecology and Environment
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    • v.40 no.2
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    • pp.120-124
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    • 2016
  • Background: Although cetrimonium bromide is widely used for its bactericidal effects, the safety of cetrimonium bromide remains controversial. Therefore, the phytotoxicity of cetrimonium bromide was tested to evaluate its acute toxicity to plants and possible toxicity to other organisms and the ecosystem. Results: The germination rates of two test species, Lactuca sativa and Brassica campestris, were significantly decreased after cetrimonium bromide treatment. Furthermore, cetrimonium bromide treatment at over 1 mg/L concentration significantly affected root elongation immediately after germination. In pot experiments with semi-mature plants, significantly decreased shoot elongation and chlorophyll content were detected in both species following cetrimonium bromide treatment. Cetrimonium bromide treatment also significantly increased the antioxidant enzyme activities of plants. Conclusion: Our results show that cetrimonium bromide is phytotoxic, and since phytotoxicity testing can imply potential toxicity in the environment, further studies of the environmental toxicity of cetrimonium bromide should be performed.

Effects of Phosphorus and Iron on the Phytotoxicity of Lettuce (Lactuca sativa L.) in Arsenic-contaminated Soil

  • Kim, Min-Suk;Min, Hyun-Gi;Kim, Jeong-Gyu
    • Ecology and Resilient Infrastructure
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    • v.5 no.1
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    • pp.1-10
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    • 2018
  • We examined the effect of simultaneous application of phosphorus (P) and iron (Fe) on the phytotoxicity of lettuce in arsenic (As) contaminated soil using response surface methodology (RSM). To stabilize As and supply nutrient into soil, Fe and P were treated, respectively. Water soluble As and P was decreased by Fe application but increased by P application. Through phytotoxicity test, the result showed that only the addition of P affected lettuce root elongation even though both P and Fe were added. The correlation coefficients between root elongation and other indices indicated that the As content in the roots seemed to be the main reason that root growth was impeded. We could verify that the former result was not a passing phenomenon and Fe was necessarily needed to protect secondary pollution by exclusive usage of P fertilizer.

Solubilizability, foliar wettability and phytotoxicity of nonionic surfactant-acetone aqueous solutions for the herbicide screening (제초제 스크리닝을 위한 계면활성제-아세톤 수용액의 용매성, 전착성 및 약해)

  • Yu, Ju-Hyun;Cho, Kwang-Yun
    • Korean Journal of Environmental Agriculture
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    • v.14 no.3
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    • pp.296-301
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    • 1995
  • For studying the role of acetone and surfactant in solution, and selecting the best surfactant for spray solution in herbicide screening, the solubilizability of 6 nonionic surfactant-acetone aqueous solutions to 18 herbicide technicals, their foliar wettability and phytotoxicity to soybean and rice plant were tested and evaluated. The solubilizability of surfactant-acetone aqueous solutions to herbicide technicals was dependent on the acetone content of solutions, and was less affected by nonionic surfactant. Foliar wettability of the surfactant solutions was good to soybean, but only polyoxyethylene lauryl ether HLB 13.6(LE-13.6) solution showed good wettability to rice plant within the concentration range of no phytotoxicity. Tween 20(0.1%), LE-13.6(0.01%) and polyoxyethylene nonylphenyl ether HLB 16.0(0.01%) solutions didn't induce phytotoxicity to soybean, and most of the surfactant solutions didn't induce phytotoxicity to rice plant. There was no surfactant that showed superior emulsifiability to various herbicide technicals, good foliar wettability to plants, and no phytotoxicity, but LE-13.6 was better than others.

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Influence of Humic or Fulvic Acid on Phytotoxicity of Bentazone (Bentazone의 약해(藥害)에 미치는 부식산(腐植酸) 및 훌브산(酸)의 영향(影響))

  • Han, Dae-Sung;Yang, Jae-E;Shin, Yong-Keon
    • Korean Journal of Environmental Agriculture
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    • v.12 no.2
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    • pp.162-168
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    • 1993
  • This research was conducted to assess the influence of humic or fulvic acid on Bentazone phytotoxicity using a bioassay with hydroponically grown cabbage (Brassica campestris subsp. napus var. pekinensis Makino). Concentrations of Bentazone in the water culture media were ranged from 0 to 32 ${\mu}M $ and those of the organic ligands were 1.0mM as a soluble carbon. Media were prepared in a complete factorial combination with pHs of 4.5, 6.5 and 8.5. The phytotoxicity indices on growth rate and dry weight decrement were employed to evaluate the effects of organic ligands on the Bentazone phytotoxicity. Humic or fulvic acid without Bentazone treatment enhanced the growth of cabbage and this effect was evident at low pH of 4.5. Bentazone led to chlorosis and necrosis on cabbage leaves resulting in the decreases of dry and fresh weights and growth rate. This phytotoxic effect was increased with Bentazone concentration and evident at low pH. At pH 4.5, dry weight was decreased about 63% with 8${\mu}$M of Bentazone treatment. Effective concentration of Bentazone causing 50% decreases in fresh weight as compared to the control was estimated to be 21${\mu}$M. Presence of organic ligand reduced the phytotoxicity of Bentazone to cabbage significantly by increasing yields and growth rates as compared to the treatment of Bentazone alone. At pH 4.5, fulvic acid reduced phytotoxicity of Bentazone upto 46%, and this efficiency of fulvic acid was better than that of humic acid under the same condition.

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Influence of Metal Oxide Particles on Soil Enzyme Activity and Bioaccumulation of Two Plants

  • Kim, Sunghyun;Sin, Hyunjoo;Lee, Sooyeon;Lee, Insook
    • Journal of Microbiology and Biotechnology
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    • v.23 no.9
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    • pp.1279-1286
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    • 2013
  • Particle size and metal species are important to both soil microbial toxicity and phytotoxicity in the soil ecosystem. The effects of CuO and ZnO nanoparticles (NPs) and microparticles (MPs) on soil microbial toxicity, phytotoxicity, and bioaccumulation in two crops (Cucumis sativus and Zea mays) were estimated in a soil microcosm. In the microcosm system, soil was artificially contaminated with 1,000 mg/kg CuO and ZnO NPs and MPs. After 15 days, we compared the microbial toxicity and phytotoxicity by particle size. In addition, C. sativus and Z. mays were cultivated in soils treated with CuO NPs and ZnO NPs, after which the treatment effects on bioaccumulation were evaluated. NPs were more toxic than MPs to microbes and plants in the soil ecosystem. We found that the soil enzyme activity and plant biomass were inhibited to the greatest extent by CuO NPs. However, in a Biolog test, substrate utilization patterns were more dependent upon metal type than particle size. Another finding indicated that the metal NP uptake amounts of plants depend on the plant species. In the comparison between C. sativus and Z. mays, the accumulation of Cu and Zn by C. sativus was noticeably higher. These findings show that metal oxide NPs may negatively impact soil bacteria and plants. In addition, the accumulation patterns of NPs depend on the plant species.

Effect of Combined Application of Molinate and Simetryn on Bioefficacy of Echinochloa crus-galli and Bidens tripartita (Molinate와 Simetryn 혼합처리가 피, 가막사리 및 벼의 생육에 미치는 영향)

  • Choi, S.Y.;Chung, B.J.;Chae, J.C.
    • Korean Journal of Weed Science
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    • v.18 no.4
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    • pp.286-294
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    • 1998
  • This study was carried out to evaluate the interaction effect between molinate(S-ethyl N,N-hexamethylene-thiocarbamate) and simetryn(N,N'-diethyl-6-(methythio)-1,3,5-triazine-2,4-diamine) on weed bioefficacy and rice phytotoxicity in green house and paddy field. Combined application of molinate and simetryn showed synergistic herbicidal effect to Echinochloa crus-galli and additive herbicidal effect to Bidens tripartita, However, it showed synergistic phytotoxicity to rice. Combined application of molinate and simetryn reduced the flooding period desirable for controlling Echinochloa crus-galli by about 2~4days as compared to single application of molinate. It was concluded that the optimum combination of molinate and simetryn was 150g and 9g to 18g a.i./10a, respectively in viewpoint of bioefficacy and phytotoxicity.

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Influence of Organic Ligands on Phytotoxicity of Paraquat (유기(有機)리간드가 Paraquat의 약해(藥害)에 미치는 영향)

  • Yang, Jae-E;Han, Dae-Sung;Shin, Yong-Keon
    • Korean Journal of Environmental Agriculture
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    • v.11 no.3
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    • pp.235-242
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    • 1992
  • Organic ligands in the environments are expected to play an important role in regulating the biotoxicity and fate of pesticides. Influences of dissolved humic and fulvic acids on the phytotoxicity of Paraquat were investigated using a bioassay with hydroponically grown rye as indicator species. Levels of Paraquat in water culture media were ranged from 0 to $12_{{\mu}M}$ and those of humic or fulvic acid were 1.0mM as a soluble carbon. Media were prepared in a factorial combination with pHs of 4.5, 6.5 and 8.5, Standard curves of necrosis days, fresh weight, and growth rates, as Phytotoxicity Indices, versus Paraquat concentrations were employed to evaluate the effects of organic ligands on phytotoxicity of paraquat. Organic ligand itself had little effect on rye growth, but Paraquat showed a high degree of toxicity. Paraquat started to show an intensive injury to rye at $0.4{\sim}0.6{{\mu}M}$ and upper critical phytotoxic concentration was estimated to be 11.0${{\mu}M}$ In the presence of organic ligands, times required to cause necrosis due to Paraquat were delayed upto 40%. Fresh weights and growth rates were upto 20% higher in treatments of organic ligands plus Paraquat than that of Paraquat alone. Results demonstrated that complexation of organic ligand with Paraquat reduced the bioabailability of Paraquat to rye.

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Uptake and Phytotoxicity of TNT in Onion Plant

  • Kim, Jaisoo;Yavuz Corapcioglu;Malcolm C. Drew
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.102-106
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    • 2003
  • The uptake of $^{14}C$-2, 4, 6-trinitrotoluene (TNT) in hydroponics was studied using onion plants. Of the total TNT mass (5 $\mu\textrm{M}$ concentration), 75% was in the roots, 4.4% in the leaves, and 21% in the external solution at 2 days, The percent distribution in roots was lower with higher concentration in the external solution, but in leaves it was comparable at all concentrations (5-500 $\mu\textrm{M}$). Root concentration factor (RCF) in hydroponics was more than 85 in constant hydroponic experiment (CHE) at 5 $\mu\textrm{M}$ and 150 in non-constant hydroponic experiment (NHE) at 5 $\mu\textrm{M}$. The maximum RCF values in the hydroponic system were greater with lower solution concentration. Transpiration stream concentration factor (TSCF) values in the present study (NHE only: 0.31-0.56) were relatively similar to the values with predicted values (0.43-0.78), increasing with higher external TNT concentration. For phytotoxicity tested in hydroponics and wet paper method, 500 $\mu\textrm{M}$ was toxic to onion plant, 50 $\mu\textrm{M}$ was non-toxic for plant growth but limited the transpiration rate, and 5 $\mu\textrm{M}$ was non-toxic as control.

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