• Journal of Ginseng Research
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• v.5 no.1
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• pp.73-84
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• 1981

Effect of environmental factors and host on the growth and outbreak of various ginseng diseases was reviewed Environmental lectors included hydrogen ion concentration, moisture content, temperature, nutrition, and microbiol populations. Age of the ginseng plants in relation to several ginseng disease occurrence was also included in order to formulate the effective control measure for ginseng diseases. Damping-off caused by Rhizoctonia, Pythium, and Phytophthora, greymold by Botrytis, sclerotinia by Scleretinia, and phytophthora blight caused by Phytophthora were usually prevalent during the early growing season of ginseng when temperature is below 20$^{\circ}C$, while anthrac se caused by Colletotrichum, alternaria blight by Alternaria, and bacterial soft rot by Erwinia were so during the latter growing season when temperature is above 25$^{\circ}C$. However, the root rot incited by Fnarium and Cylindrocarpon caused severe damages throughout the growing season. Growth range of the temperature for a pathogen was highly related to the corresponding disease outbreak. Hydrogen ion concentration was highly related to the outbreak of sclerotinia, root rot, and red rot. Most severe outbreak of those diseases where the soil acidity was pH 4.7, pH 6.5- 7.5, and pH6.0-6.5, respectively. Nitrogen content in the soil was also related to outbreak of root rot and red rot. More red rot occurred where NH,-nitrogen is above 30 ppm and more root rot obtained when excessive nitrogen fertilizer applied. Yellow necrosis apparently was related to magnesium especially its ratio with potassium or calcium content in a soil. Fusarium Population showed significant .relations to missing rate of ginseng Plants in a Implanting ginseng field, while that of total bacteria showed similar relations in all ginseng field, However, in six year old ginseng fields, the more the Streptomyces population was, the less the Fusarium obtained. Consequently, less missing rate observed in a field where Streptomyces population was high. Damping-off, root rot, Rhytophthor a blight were mose severe on the nursery and on 2-3 years old ginseng plants, whereas sclerotinia, and grey cod, alteraria blight, anthracnose were severe on 4-6 years old ginseng plants. Root rot caused by Fusarium and Erwinia, however, was also severe regardless of the age of the plants when the roots were injured. Therefore, for the effective control of ginseng root rot most careful control of the disease during the early year should be rendered.

• The Korean Journal of Pesticide Science
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• v.12 no.3
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• pp.270-276
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• 2008

Through the agar dilution method on V-8 juice agar, sensitivity of Phytophthora capsici causing pepper Phytophthora blight to metalaxyl was investigated by using isolates obtained from infected pepper plants during 3 years from 2005 to 2007. By the lapse of time, $EC_{50}$ value to metalaxyl was decreased, showing 1.45, 0.83, and $0.32{\mu}g\;mL^{-1}$ in 2005, 2006, and 2007. None of 2007 isolates was found to be resistant to metalaxyl. Compared the sensitivity of P. capsici isolates to metalaxyl with those to mandipropamid and dimethomorph, there is not a cross resistance response between metalaxyl and mandipropamid/dimethomorph. The resistance to metalaxyl in pepper Phytophthora blight pathogen was not related with the mycelial growth on V-8 agar medium and the pathogenicity on pepper plants.

• Korean Journal of Environmental Agriculture
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• v.15 no.3
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• pp.289-295
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• 1996

This study was attempted to select an antagonist against Phytophthora blight of red-pepper caused by Phytophthora capsici. The three strains, A-35, A-67 and A-183 were isolated from the rhizosphere in soil where red-pepper had been cultivated continuously for a long time, and the strain A-83 was estimated to be the strongest antagonist against P. capsici. The A-183 strain was identified as a strain of Pseudomonas sp., showing the maximum antifungal activity, when cultured at $30^{\circ}C$ for 5 days in the potato extract medium(pH 6.5) containing 2.0% mannitol and 0.3% peptone.

• The Korean Journal of Pesticide Science
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• v.14 no.4
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• pp.463-472
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• 2010

It was conducted to investigate the control efficacy of carboxylic acid amide (CAA) fungicides, such as benthiavalicarb, iprovalicarb, dimethomorph and mandipropamid, against pepper Phytophthora blight caused by P. capsid in the laboratory and the field. The fungicides inhibited mycelial growth and direct sporangium germination of P. capsid strongly, while there was no activity of all fungicides against zoospore release from sporangium. In greenhouse test, they showed the good protective and curative effect against pepper Phytophthora blight. Benthiavalicarb applied at $100{\mu}g\;mL^{-1}$ 7 days before inoculation prevented pepper Phytophthora blight by 100%, even though the zoosporangiurn suspension of P. capsid adjusted to not only $5{\times}10^3$ zoosporangia $mL^{-1}$ but also $1{\times}10^5$ zoosporangia $mL^{-1}$ was inoculated by soil-drenching. Except for dimethomorph, the other fungicides showed an excellent control activity over 2 years from 2009 to 2010 in the field test. The control value of dimethomorph applied at $250{\mu}g\;mL^{-1}$ was low, 27.2% in 2009, but that of dimethomorph applied even at $125{\mu}g\;mL^{-1}$ was high, 89.5% in 2010. All the fungicides showed good inhibitory effect on the mycelial growth and the direct germination of zoosporangiurn, and controlled pepper Phytophthora blight preventively and curatively, can be used to establish the spray system for control1ing the pepper disease.

• Korean Journal Plant Pathology
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• v.10 no.3
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• pp.192-196
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• 1994

Two hundred and fourty-seven isolates of Phytophthora infestans obtained from infested potato leaves from the spring of 1991 to the fall of 1993 from potato fields in various regions of Korea were tested for their fungicides resistances. A total of 20.9% isolates were not suppressed at 50 ppm of metalaxyl in 1991, but isolates from 1993 were suppressed at 50 ppm of metalaxyl. Ten resistant isolates and 10 susceptible isolates to metalaxyl were selected and tested against oxadixyl, fosetyl-Al, and phosphorous acid. Effectiveness of these chemicals were no better than that of metalaxyl. Dimethomorph suppressed all isolates at 1 $\mu\textrm{g}$/ml suggesting that it might be a potential chemical to control Phytophthora infestans. Mating types of all isolates from diseased leaves in 1993 turned out to be A2 type.

• Journal of Ginseng Research
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• v.33 no.4
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• pp.311-315
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• 2009

Potassium phosphonate inhibited less mycelial growth of Phytophthora cactorum in vitro than metalaxyl-M. But the net protection rate in leaflet test and whole plant pot test was greater in potassium phosphonate than metalaxyl-M. P. ginseng appeared to have an induced resistance against P. cactorum with phosphonate around 50~100 ppm.

• Journal of Microbiology
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• v.39 no.2
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• pp.126-132
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• 2001

For rapid and secure differentiation of P. infestans from other Phytophthora species, two fragments obtained from randomly amplified polymorphic DNA (RAPD) profiles were selected as markers. Also, primers for in polymerase chain reaction (PCR) to detect P. infestans specifically were developed by analyzing the sequences of ITSII regions in rDNA of Phytophthora species. The primers, PISP-1 and ITS3 amplified a single. Fragment 450 bp of about in P. infestans, but not in other fungal or bacterial isolates. Annealing temperatures and template DNA quantities were varied for the optimization of PCR conditions. From the result of the PCR detection study, species-specific primers were selected under annealing temperatures ranging from 55$^{\circ}C$ to 61$^{\circ}C$, and template DNA levels ranging from 10 pg to 100 ng.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.117.1-117
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• 2003

Phytophthora blight, caused by P. capsici, is very important disease of pepper. Many fungicides to control of Phytophthora blight have been developed, but most of fungicides disappeared in short periods. Nowadays dimethomorph was known as one of the most effective to control of this disease. P. capsici isolates from pepper fields were collected and surveyed their growth in dimethomorph amended V8 medium in order to evaluate their fungicides resistance. The fungicide resistant isolates were not founded among them. Most of the sensitive isolates were inhibited perfectly in V8 medium amended with 10ppm dimethomorph. Mutants of P. capsici by dimethomorph, was grown very well in 250ppm. The difference of pathogenicity, colony morphology, drug response, RT-PCR results was identified between sensitive and resistance isolates. This study should be provided a basic information about the occurrence of dimethomorph resistant isolates and genetic changes in P. capsici population.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.103.2-104
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• 2003

Ginseng (Panax ginseng) is one of the most widely cultivated medicinal herbs in Korea. During 3 or 5 years cultivation of ginseng, yield losses can reach as high as 30-60％ due to numerous diseases in Korea. Among 106 Bacillus strains isolated from various plant internal roots, we selected three promising biocontrol agents by screening against root rot caused by Cylindrocarpon destructan in a greenhouse. Preinoculation of selected isolates to seed or one-year-old root resulted in stimulation of shoot and/or root growth of seedlings, and control of root rot in infested soils with Cylindronrpon destructans (P=0.05). Furthermore, drenching of selected isolates on seedling-growing pots reduced the incidence of Phytophthora blight when the seedlings were challenged with zoospores of Phytophthora cactorum (P=0.05). However, isolates B1141 and B1142 did not show any antifungal activity against various soilborne pathogens while B1146 did in vitro. Our results provide an insight that rhizobacteria can induce resistance against various plant diseases on ginseng even if any resistant breeds have been unknown on ginseng yet.

• The Plant Pathology Journal
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• v.26 no.2
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• pp.178-184
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• 2010

Four major diseases of chili pepper including two fungal diseases, anthracnose (Colletotrichum acutatum) and Phytophthora blight (Phytophthora capsici), and two bacterial diseases, bacterial wilt (Ralstonia solanacearum) and bacterial spot (Xanthomonas campestris pv. vesicatoria), were investigated under future climate-change condition treatments in growth chambers. Treatments with elevated $CO_2$ and temperature were maintained at $720ppm{\pm}20ppm$ $CO_2$ and $30^{\circ}C{\pm}0.5^{\circ}C$, whereas ambient conditions were maintained at $420ppm{\pm}20ppm$ $CO_2$ and $25^{\circ}C{\pm}0.5^{\circ}C$. Pepper seedlings or fruits were infected with each pathogen, and then the disease progress was evaluated in the growth chambers. According to paired t-test analyses, bacterial wilt and spot diseases significantly increased by 24% (p=0.008) and 25% (p=0.016), respectively, with elevated $CO_2$ and temperature conditions. On the other hand, neither Phytophthora blight (p=0.906) nor anthracnose (p=0.125) was statistically significant. The elevated $CO_2$ and temperature accelerated the progress of bacterial wilt by two days and bacterial spot by one day compared to the ambient treatment. Temperature regime studies of the diseases without changes in $CO_2$ confirmed that the accelerated bacterial disease progress was mainly due to the increased temperature rather than the elevated $CO_2$ conditions.