• Title/Summary/Keyword: physiological stress

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The Effects of Skin Pigmentation on Physiological Factors of Thermoregulation and Grazing Behaviour of Dairy Goats in a Hot and Humid Climate

  • Darcan, Nazan Koluman;Cankaya, Soner;Karakok, Serap Goncu
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.5
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    • pp.727-731
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    • 2009
  • The aim of this study was to understand the effects of skin pigmentation on physiological parameters of thermoregulation and grazing behaviour of dairy goats in a hot and humid climate. The study used 26 crossbred Saanen yearling goats (95% Saanen+5% Local Hair Breed). The animals were raised at semi-intensive private farms in Adana (36 59'N, 35 18'E). Groups were selected 2 d before the start of observations. Goats were categorized as predominantly pigmented (P) skin and unpigmented (UP) skin. All observations and measurements were collected on grassland during the grazing period of June and July 2007 (60 d). Air temperature and relative humidity were recorded at 10 min intervals by a portable data logger. The physiological data (rectal temperature, respiration and pulse rate, and skin temperatures from head and udder) were recorded twice weekly in the morning (07:00-08:00); at midday (13:00-14:00); and in the evening (18:00-19:00). Additionally, the activity of the animals was observed and classified (eating, ruminating, drinking, standing, walking, lying) for 12 h during the day twice weekly, using a portable camera system linked directly to a computer. Panting behaviour was also observed. According to the THI values, the experimental goats were subjected to stressful conditions. The pigmented goats had significantly lower rectal temperatures (39.68 vs. $29.89^{\circ}C$), pulse rate (74.08 vs. 84.10 beat/min) and respiration rate (65.65 vs. 88.23 breath/min.) compared with unpigmented goats at midday when the THI exceeded 92. The rectal, head and udder temperatures, pulse and respiration rates of the non-pigmented group exceeded $40^{\circ}C$, $37^{\circ}C$, $37.5^{\circ}C$, 84 beats/min and 78 breaths/min, respectively. Higher activity was observed among pigmented compared with unpigmented goats. Unpigmented goats grazed (4.3 vs. 5.6 h), ruminated (2.0 vs. 2.4 h), and stood (0.8 vs. 1.2 h) less, but lay down (2.2 vs. 1.8 h) more than pigmented goats. The data obtained in this experiment support the hypothesis that unpigmented goats are more adversely affected by climatic stress, likely due to their decreased activity and increased water consumption, as demonstrated by previous studies.

Physiological Responses of Chilling - Tolerant and Susceptible Rice Cultivars during Chilling Stress and Subsequent Recovery (저온스트레스와 회복기간중 저온 내성 및 감수성 벼 품종의 생리적 반응)

  • Kuk, Yong-In;Shin, Ji-San;Lee, Hee-Jae;Guh, Ja-Ock
    • Korean Journal of Environmental Agriculture
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    • v.20 no.2
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    • pp.93-98
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    • 2001
  • A chilling-tolerant japonica-type and a chilling-susceptible indica-type of rice (Oryza sativa L.) plants were compared with respect to various physiological parameters during chilling and subsequent recovery. The japonica-type and the indica-type of rice cultivars used were Ilpumbyeo and Taebaekbyeo, respectively. The two rice cultivars exhibited little or no differences in the changes of leaf fresh weight and chlorophyll content during chilling at $5^{\circ}C$ for 3 days. During subsequent recovery at $25^{\circ}C$, however, Ilpumbyeo restored its growth more rapidly than Taebaekbyeo. Since the changes of relative water content, malondialdehyde production, an estimate of lipid peroxidation, and chlorophyll fluorescence were significantly different in the two rice cultivars during the chilling and subsequent recovery, they were found to be more sensitive physiological parameters than fresh weight and chlorophyll content. However, the differences in relation to water content, malondialdehyde production, and chlorophyll fluorescence between the two rice cultivars were smaller during chilling than those during subsequent recovery. These results suggest that relative water content, malondialdehyde production, and chlorophyll fluorescence could widely be used as important physiological parameters for screening chilling-tolerant plants.

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Development of Korean SPAR(Soil-Plant-Atmosphere-Research) System for Impact Assessment of Climate Changes and Environmental Stress (기후변화 및 환경스트레스 영향평가를 위한 한국형 SPAR(Soil-Plant-Atmosphere-Research) 시스템의 개발)

  • Sang, Wan-Gyu;Kim, Jun-Hwan;Shin, Pyong;Baek, Jae-Kyeong;Lee, Yun-Ho;Cho, Jung-Il;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.3
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    • pp.187-195
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    • 2019
  • The needs for precise diagnostics and farm management-decision aids have increased to reduce the risk of climate change and environmental stress. Crop simulation models have been widely used to search optimal solutions for effective cultural practices. However, limited knowledge on physiological responses to environmental variation would make it challenging to apply crop simulation models to a wide range of studies. Advanced research facilities would help investigation of plant response to the environment. In the present study, the sunlit controlled environment chambers, known as Korean SPAR (Soil-Plant-Atmosphere-Research) system, was developed by renovating existing SPAR system. The Korean SPAR system controls and monitors major environmental variables including atmospheric carbon dioxide concentration, temperature and soil moisture. Furthermore, plants are allowed to grow under natural sunlight. Key physiological and physical data such as canopy photosynthesis and respiration, canopy water and nutrient use over the whole growth period are also collected automatically. As a case study, it was shown that the Korean SPAR system would be useful for collection of data needed for understanding the growth and developmental processes of a crop, e.g., soybean. In addition, we have demonstrated that the canopy photosynthetic data of the Korean SPAR indicate the precise representation of physiological responses to environment variation. As a result, physical and physiological data obtained from the Korean SPAR are expected to be useful for development of an advanced crop simulation model minimizing errors and confounding factors that usually occur in field experiments.

Effect of Experimental Warming on Physiological and Growth Responses of Larix kaempferi Seedlings (실외 온난화 처리에 따른 낙엽송 묘목의 생리 및 생장 반응)

  • An, Jiae;Chang, Hanna;Park, Min Ji;Han, Seung Hyun;Hwang, Jaehong;Cho, Min Seok;Son, Yowhan
    • Journal of Climate Change Research
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    • v.7 no.1
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    • pp.77-84
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    • 2016
  • Seedling stage is particularly important for tree survival and is easily influenced by warming. Therefore, air temperature being increased due to climate change may affect physiological traits and growth of seedlings. This study was conducted to investigate the physiological and growth responses of Larix kaempferi seedlings to open-field experimental warming. 1-year-old and 2-year-old L. kaempferi seedlings were warmed with infrared lamps since April 2015 and April 2014, respectively. The seedlings in the warmed plots were warmed to maintain the air temperature to be $3^{\circ}C$ higher than that of the control plots. Physiological responses (stomatal conductance, transpiration rate, net photosynthetic rate and total chlorophyll content) and growth responses (root collar diameter (RCD), height and biomass) to experimental warming were measured. Physiological and growth responses varied with the seedling ages. For 2-year-old L. kaempferi seedlings, stomatal conductance, transpiration rate and net photosynthetic rate decreased following the warming treatment, whereas there were no changes for 1-year-old L. kaempferi seedlings. Meanwhile, total chlorophyll content was higher in warmed plots regardless of the seedling ages. Net photosynthetic rate linked with stomatal conductance also decreased due to the drought stress and decrease of photosynthetic efficiency. In response to warming, RCD, height and biomass did not show significant differences between the treatments. It seems that the growth responses were not affected as much as physiological responses were, since the physiological responses were not consistent, nor the warming treatment period was enough to have significant results. In addition, multifactorial experiments considering the impact of decreased soil moisture resulting from elevated temperatures is needed to explicate the impacts of a wide range of possible climate change scenarios.

Control Indian meal moth Plodia interpunctella by gas treatment

  • Han, Gyung Deok;Kwon, Hyeok;Jin, Hyun Jung;Kum, Ho Jung;Kim, Bo Hwan;Kim, Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.45-45
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    • 2017
  • The Indian meal moth, Plodia interpunctella, is one of the most important pests of stored food in the food processing industry worldwide. To control the Indian meal moth, methyl bromide, phosphine, high carbon dioxide, sulfuryl fluoride and plant essential oil fumigation have been considered. However, these treatments have disadvantages. For example, depleting the ozone layer, showing resistance in insect, low control efficacy or need high cost for treatment. Chlorine dioxide ($ClO_2$) is strong disinfectant and insecticide. The gas caused a malfunction in enzymes. The oxidative stress induced by $ClO_2$ gas treatment damaged to a physiological system and all life stages of P. interpunctella. The gaseous $ClO_2$ is a convincing alternative to methyl bromide for controlling P. interpunctella. The gaseous $ClO_2$ was generated by a chlorine dioxide generator (PurgoFarm Co., Ltd., Hwasung, Korea). It generated highly pure $ClO_2$ gas and the gas blown out through a vent into a test chamber. Gas entry to the chamber was automatically controlled and monitored by a PortaSene II gas leak detector (Analytical Technology, Collegeville, PA, USA). The properly prepared eggs, larvae, pupae, and adults of P. interpunctella were used in this experiment. Data were analyzed using SAS 9.4. Percentage data were statistically analyzed after arcsine-root transformation. Analysis of variance was performed using general linear model, and means were separated by the least significant difference test at P < 0.05. Fumigation is an effective management technique for controlling all stages of P. interpunctella. We found that $ClO_2$ gas treatment directly effects on egg, larvae, pupae and adults of P. interpunctella. The gas treatment with proper concentration for over a day achieved 100 % mortality in all stages of P. interpunctella and short time treatment or low concentration gas treatment results showed that the egg hatchability, pupation rate, and adult emergency rate were lowered compare with untreated control. Also, abnormal pupae or adult rate were increased. Gaseous $ClO_2$ treatment induced insecticidal reactive oxygen species (ROS), and it resulted in fatal oxidative stress in P. interpunctella. Taken together, these results showed that exposure proper concentration and time of the gas control all stages of P. interpunctella by inducing fatal oxidative stress. Further studies will be required to apply the gas treatment under real-world condition and to understanding physiological reaction in P. interpunctella caused by oxidative stress.

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Effects of Soil Water Content on Growth and Antioxidative Enzymes of Tomato Plug Seedlings (토양 수분함량이 토마토 묘의 생육 및 항산화 효소에 미치는 영향)

  • Kim, D.E.;Kang, J.K.;Shin, Y.A.;Hong, S.J.;Lee, W.Y.;Woo, Y.H.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.21 no.2
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    • pp.59-69
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    • 2019
  • This study was carried out to investigate effects of soil water content on growth and antioxidative enzymes activity of tomato seedlings during the nursery period. The water stress significantly damages morphological, physiological, and biochemical activities in plants. The seedlings planted with soil on the tray were irrigated and categorized into 3 groups with 30 g, 40 g, and 50 g of plant-soil weight. After then, the changes in weight of the soil and the seedling were measured evey 2 hours for 4 days and the leaf temperature was measured with the thermal-camera at the same time. The antioxidant enzymes were measured to determine the level of stress using all of the seedling samples. The result showed that the decrease of soil weight in the day time was faster than that in the night time, but there was no significant difference in the weight loss of the seedlings and soil among the groups. However, the group with 50 g of wight showed the highest SOD and POD contents. This is considered that the continuously wet soil on the root zone of the seedlings caused more stress for the seedlings. Therefore, it is concluded that the excess moisture content causes stress to stimulate the secretion of antioxidant enzymes, and the effect of stress is required to be analyzed comprehensively using environmental data and also the physiological data that are collected over a longer period.

A Comparative Study of Depression and Stress Related Life Events among Women in the Menopausal Stage (우울증과 생활사건 스트레스에 대한 폐경전${\cdot}$후 비교)

  • Chung, Eun-Soon
    • Journal of Korean Academy of Nursing
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    • v.30 no.4
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    • pp.956-966
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    • 2000
  • The purpose of this study was to examine the comparative between depression and stress related to life events among women in the menopausal stage. Menopausal stages were divided into two groups: Pre and post-menopausal stages. The degree of depression and stress related to life events between pre and post menopausal women were compared to each other. Women, aged between 41 and 59 years, answered self-reported questionnaires which included Zung's depression scale and life events scale modified by Lee (1984). Findings were as follows; 1) The mean score of premenopausal women who experienced depression was 39.66, and for post-menopausal women the score was 41.45. There was no significant differences in depression levels between pre and post menopausal group.s (t=-1.55, p=.122). 2) Menopausal women experienced low levels of stress related to life events. There were no significant differences between pre and post menopausal groups(t=.527, p>.05). Both pre and post menopausal groups were highly concerned about education issues of their children and disharmony between couples. 3) There was a significant relationship between depression and stress related to life events among post-menopausal groups (r=.22, p<.01). Based on the findings of this study, the menopausal depression was associated with stress related to life events, especially among post-menopausal women. Feelings of lost fertility and feminine attributies result in menopausal depression, which is significantly correlated with women's negative perception of their life events. Therefore, nursing intervention needs to develop to help reduce the levels of depression and overcome their negative perception of the menopausal experience. Nurses should develop nursing strategies to help menopausal women to have positive perceptions and enhance quality of life by assisting their adaptability to physiological and psychological changes related to menopause.

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Practice in Relaxation Techniques (이완요법의 실제)

  • Joe, Sook-Haeng
    • Korean Journal of Psychosomatic Medicine
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    • v.9 no.1
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    • pp.93-102
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    • 2001
  • The relaxation response is a state of profound rest, creates physiological responses directly opposite to the stress response. The relaxation response can be used to counteract the harmful effects of stress. The relaxation response can be elicited by a number of techniques such as diaphragmatic breathing, meditation, progressive muscle relaxation, autogenic training, biofeedback, etc. These relaxation methods in any mental or physical conditions associated with distress and even in normal people have useful benefits for stress control and health enhancement. These relaxation techniques are but one part of a comprehensive stress management program, through regular and continuous practice appropriate for each person, they will make an effective role in stress management. In this review, author reviewed how to practically use meditation, progressive muscle relaxation and autogenic training, in more detail. In the treatment of various stress-related disease, especially in psychiatric disorders, the relaxation technique may be a useful complement to conventional treatment and serves as an intervention between stress and disease.

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A Bioactive Fraction from Streptomyces sp. Enhances Maize Tolerance against Drought Stress

  • Warrad, Mona;Hassan, Yasser M.;Mohamed, Mahmoud S.M.;Hagagy, Nashwa;Al-Maghrabi, Omar A.;Selim, Samy;Saleh, Ahmed M.;AbdElgawad, Hamada
    • Journal of Microbiology and Biotechnology
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    • v.30 no.8
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    • pp.1156-1168
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    • 2020
  • Drought stress is threatening the growth and productivity of many economical crops. Therefore, it is necessary to establish innovative and efficient approaches for improving crop growth and productivity. Here we investigated the potentials of the cell-free extract of Actinobacteria (Ac) isolated from a semi-arid habitat (Al-Jouf region, Saudi Arabia) to recover the reduction in maize growth and improve the physiological stress tolerance induced by drought. Three Ac isolates were screened for production of secondary metabolites, antioxidant and antimicrobial activities. The isolate Ac3 revealed the highest levels of flavonoids, antioxidant and antimicrobial activities in addition to having abilities to produce siderophores and phytohormones. Based on seed germination experiment, the selected bioactive fraction of Ac3 cell-free extract (F2.7, containing mainly isoquercetin), increased the growth and photosynthesis rate under drought stress. Moreover, F2.7 application significantly alleviated drought stress-induced increases in H2O2, lipid peroxidation (MDA) and protein oxidation (protein carbonyls). It also increased total antioxidant power and molecular antioxidant levels (total ascorbate, glutathione and tocopherols). F2.7 improved the primary metabolism of stressed maize plants; for example, it increased in several individuals of soluble carbohydrates, organic acids, amino acids, and fatty acids. Interestingly, to reduce stress impact, F2.7 accumulated some compatible solutes including total soluble sugars, sucrose and proline. Hence, this comprehensive assessment recommends the potentials of actinobacterial cell-free extract as an alternative ecofriendly approach to improve crop growth and quality under water deficit conditions.

Changes of Renal Peripheral Benzodiazepine Receptor in the Stress/Anxiety Response

  • Ha, Jeoung-Hee;Lee, Kwang-Hun;Cheung, Seung-Douk;Park, Hyung-Bae;Lee, Maan-Gee;Choi, Hyoung-Chul;Sohn, Uy-Dong;Lee, Kwang-Youn;Kim, Won-Joon
    • The Korean Journal of Physiology and Pharmacology
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    • v.1 no.5
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    • pp.523-528
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    • 1997
  • Peripheral benzodiazepine receptor(PBR) has been indentified in various peripheral tissues including kidney. The physiological and pharmacological functions of PBR are still uncertain, althought it has been suggested that these are associated with the regulation of stress/anxiety response. Diazepam progeny, which were exposed to diazepam perinatally, was reported to be an animal model of chronic anxiety. However, PBR in the diazepam progenies are not known yet. In the present study, therefore, we examined the changes of PBR in the stress/anxiety response. Dams of rats were given injection of diazepam or vehicle during puerperium. Diazepam progenies showed increased level of anxiety on the performance of elevated plus maze, and increased Bmax of PBR. Saturation experiments followed by scatchard analysis of the results showed that the increase in the density of PBR and the affinity of the PBR remained unchanged. Forced swim stress increased anxiety on the plus maze in both groups of rats. In contrast to control, diazepam progenies did not show further upregulation of renal PBR immediately after swimming stress, but still higher than control. From the above results, it may be concluded that upregulation of renal PBR is associated with chronic anxiety as well as stress-induced response.

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