• Title/Summary/Keyword: phylogenetic characteristics

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Isolation and Phylogenetic Analysis of Orchid Rotting Fungus, Fusarium sp. KS-01 (난 썩음병균 Fusarium sp. KS-01의 분리 및 계통학적 분석)

  • Park, In-Jae;Shin, Kwang-Soo
    • The Korean Journal of Mycology
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    • v.33 no.2
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    • pp.92-94
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    • 2005
  • A orchid rotting fungus was isolated and identified. The isolate was consistent with the genus Fusarium in morphological and cultural characteristics. The partial 18S rRNA sequence of the isolate showed high similarity with anamorph or telemorph of Fusarium and other Fusarium species. In phylogenetic analysis, the isolates was poorly related to other Fusarium species. The isolate closely related to Fusarium sp. LP-A2/3.

Phylogenetic Status of an Unrecorded Species of Curvularia, C. spicifera, Based on Current Classification System of Curvularia and Bipolaris Group Using Multi Loci

  • Jeon, Sun Jeong;Nguyen, Thi Thuong Thuong;Lee, Hyang Burm
    • Mycobiology
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    • v.43 no.3
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    • pp.210-217
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    • 2015
  • A seed-borne fungus, Curvularia sp. EML-KWD01, was isolated from an indigenous wheat seed by standard blotter method. This fungus was characterized based on the morphological characteristics and molecular phylogenetic analysis. Phylogenetic status of the fungus was determined using sequences of three loci: rDNA internal transcribed spacer, large ribosomal subunit, and glyceraldehyde 3-phosphate dehydrogenase gene. Multi loci sequencing analysis revealed that this fungus was Curvularia spicifera within Curvularia group 2 of family Pleosporaceae.

Characterization of Paecilomyces variotii and Talaromyces amestolkiae in Korea Based on the Morphological Characteristics and Multigene Phylogenetic Analyses

  • Nguyen, Thi Thuong Thuong;Paul, Narayan Chandra;Lee, Hyang Burm
    • Mycobiology
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    • v.44 no.4
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    • pp.248-259
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    • 2016
  • During fungal diversity surveys of the order Eurotiales in Korea, two fungal strains, EML-DG33-1 and EML-NCP50, were isolated from samples of rat dung and fig tree leaf collected at a garden located in Gwangju in 2014. To complete the National Species List of Korea, it is a prerequisite to verify whether many questionable species, which were previously recorded but not confirmed, indeed present in Korea. Herein, the isolates were confirmed as undescribed species, Paecilomyces variotii and Talaromyces amestolkiae based on the combination of morphological and phylogenetic analyses of multigenes including the rDNA internal transcribed spacer, ${\beta}-tubulin$, and RNA polymerase II subunit 2.

New record of the unstalked crinoid Tropiometra macrodiscus (Crinoidea: Comatulida: Tropiometridae) from Korea

  • Kim, Philjae;Lee, Taekjun;Shin, Sook
    • Journal of Species Research
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    • v.11 no.1
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    • pp.22-28
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    • 2022
  • Two crinoid specimens of genus Tropiometra were collected from Busan and Tongyeong by SCUBA diving on August 2010 and October 2011, respectively. The specimens were identified as Tropiometra macrodiscus (Hara, 1895), which belongs to the family Tropiometridae of superfamily Tropiometroidea. The genus Tropiometra AH Clark, 1907 comprises four species worldwide at present, and it has not been reported in Korea. Tropiometra macrodiscus was first described by Hara (1895) in Japan. It is difficult to distinguish T. macrodiscus from T. afra (Hartlaub, 1890), there has been confusion with examination of their phylogenetic positions in crinoid morphological classification. Despite this, T. macrodiscus can be distinguished from T. afra based on longer arms, stouter whole-body parts, magnificently larger numbers of cirrus, and numerous segments. The morphological characteristics of T. macrodiscus collected in Korea have been described, and DNA barcode region representing cytochrome c oxidase subunit I was obtained for its molecular phylogenetic analysis.

Complete mitochondrial genome of freshwater goby Rhinogobius cliffordpopei (Perciformes, Gobiidae): genome characterization and phylogenetic analysis

  • Zhong, Liqiang;Wang, Minghua;Li, Daming;Tang, Shengkai;Zhang, Tongqing;Bian, Wenji;Chen, Xiaohui
    • Genes and Genomics
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    • v.40 no.11
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    • pp.1137-1148
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    • 2018
  • Freshwater gobies Rhinogobius cliffordpopei and R. giurinus are invasive species with particular concern because they have become dominant and were fierce competitors in the invaded areas in Yunnan-Guizhou Plateau (southwest of China). Information about genetic characteristics of R. giurinus have been published, but there were still no relevant reports about R. cliffordpopei. In present study, the complete mitochondrial genome of R. cliffordpopei was determined, which was 16,511 bp in length with A+T content of 51.1%, consisting of 13 protein-coding genes, 22 tRNAs, 2 ribosomal RNAs, and a control region. The gene composition and the structural arrangement of the R. cliffordpopei complete mtDNA were identical to most of other teleosts. Phylogenetic analyses placed R. cliffordpopei in a well-supported monophyletic cluster with other Rhinogobius fish. But the phylogenetic relationship between genus Rhinogobius and Tridentiger remained to be resolved.

Comparison Between Phylogenetic Relationships Based on 18S rDNA Sequences and Growth by Salinity of Chlorella-like Species (Chlorophyta)

  • Lee, Hye-Jung;Hur, Sung-Bum
    • Fisheries and Aquatic Sciences
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    • v.15 no.2
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    • pp.125-135
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    • 2012
  • This study was carried out to understand the correlation between phylogenetic relationships based on 18S rDNA sequences and growth by salinity of Chlorella-like species. The 18S rDNA sequences of 71 Chlorella-like species which were mainly collected from Korean waters were analyzed. The 18S rDNA sequences of Chlorella-like species were divided into three groups (group A, B and C) and group B was further divided into three subgroups (subgroup B-1, B-2 and B-3). Thirty-seven Chlorella-like species in group A grew well at high salinity (32 psu) but the other groups grew well in freshwater. The sequence identities of the species in group A and B were 97.2-99.5%, but those of 6 species in group C ("Chlorella" saccharophila), which contained group I intron sequences region were 75.0-75.4%. Two representative species of each group were cultured at different salinities (0, 16 and 32 psu) to examine the correlation between the molecular phylogenetic groups and the phenotypic characteristics on cell growth and size by different salinities. The size of cell cultured at different salinities varied according to the species of each molecular phylogenetic group. The size of "Chlorella" saccharophila in group C was bigger and more obviously elliptical rather than that of the other Chlorella-like species. Considering the results on molecular and phenotypic characteristics, the group A and B belonged to Chlorellaceae, but group C was distinctly different from them.

Four Species of Montagnulaceae Unrecorded in Korea and Isolated from Plant Litter in Freshwater

  • Goh, Jaeduk;Mun, Hye Yeon;Oh, Yoosun;Chung, Namil
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.263-270
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    • 2016
  • Freshwater is a diverse and complex environment for fungi and provides several types of habitat including plant litter, sediment, and carcasses. We collected plant litter from the main stream and branch streams of Nakdong River, Sohancheon in Samcheok, and Geumoreum in Jeju. From several samples of plant litter, we isolated 8 fungal strains belonging to 4 Montagnulaceae species unrecorded in Korea: Paraconiothyrium archidendri, Paraphaeosphaeria sporulosa, Paraphaeosphaeria michotii, and Paraphaeosphaeria viridescens. These fungi were identified by phylogenetic analysis using the internal transcribed spacer (ITS) region and examination of morphological characteristics. Montagnulaceae is known as one of the families in Pleosporales and includes coniothyrium-like fungi. In this study, we described phylogenetic analysis and mycological characteristics of these species, and this is the first report of these taxa in Korea.

First Report of Arbuscular Mycorrhizal Fungi in Korea: Acaulospora delicata,Dentiscutata colliculosa, and Racocetra alborosea.

  • Park, Hyeok;Ka, Kang-Hyeon;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.209-215
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    • 2020
  • Spores of arbuscular mycorrhizal fungi (phylum Glomeromycota) were isolated from rhizosphere soil collected in Korea. We identified the morphological characteristics of the spores and performed a phylogenetic analysis using the rDNA 18S, 5.8S, and 28S regions. To the best of our knowledge, we confirm the presence of three species of glomeromycotan fungi previously not reported in Korea, namely Acaulospora delicata, Dentiscutata colliculosa, and Racocetra alborosea. We described the morphological characteristics and results of phylogenetic analysis of these species.

Acaulospora jejuensis, a New Species in Glomeromycota from Korea

  • Park, Hyeok;Ka, Kang-Hyeon;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.49 no.4
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    • pp.425-431
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    • 2021
  • New species of arbuscular mycorrhizal fungi (Glomeromycota), Acaulospora jejuensis, was isolated from rhizosphere soils of Miscanthus sinensis in the grassland in Jeju Island of Korea. The species was identified using the morphological characteristics of the spores and the molecular phylogenetic analysis using partial DNA sequences from small subunit rDNA (SSU), internal transcribed spacer (ITS), and large subunit rDNA (LSU). Phylogenetic analysis placed the fungal species in a distinct clade within genus Acaulospora. Also, the species exhibited the morphological characteristics distinct from the other members of the genus. Therefore, Acaulospora jejuensis was described as a novel species from Korea.

Report on Tuber huidongense, a Truffle Species Previously Unrecorded in Korea

  • Park, Hyeok;Gwon, Ju-Hui;Lee, Jong-Chul;Kim, Hyun Suk;Oh, Deuk Sil;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.48 no.4
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    • pp.505-510
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    • 2020
  • The fruiting bodies and roots of a Tuber species were collected from the rhizosphere of Quercus dentata in Pohang, Korea. The morphological characteristics of the ascoma and ectomycorrhizal roots were studied, and phylogenetic analyses were performed using the sequences of the internal transcribed spacer, large subunit rDNA, and β-tubulin DNA. Based on the features of the fruiting bodies, the species was identified to be Tuber huidongense, which has not been reported earlier in Korea. The morphological characteristics and phylogenetic analysis of T. huidongense are described in the present study.