• 한국식물병리학회지
• /
• 제13권4호
• /
• pp.239-243
• /
• 1997

1990년 8월 충북 보은의 참깨 밭에서 전형적인 엽화병(葉化病; phyllody disease)이 발생하였다. 병징은 꽃이 잎처럼 녹색으로 변하고, 이병엽은 정상엽보다 작고 잔가지가 마치 빗자루 모양으로 총생하였고 개화 및 결실이 되지 않았다. 투과 전자현미경으로 병든 잎의 사관요소에서 phytoplasma가 존재하는 것을 확인하였다. 병든 잔가지에서 추출한 DNA를 중합효소 연쇄반응으로 분석한 결과 대추나무 빗자루병에 걸린 나무에서 증폭되는 DNA와 같은 크기의 band가 관찰되었다. 따라서 위의 시료는 phytoplasma 벙으로 확인 되었으며 참깨 엽화병으로 명명하였다. 또한 참깨 엽화병을 일으키는 phytoplasma와 대추 나무 빗자루병 phytoplasma를 PCR 증폭 및 제한효소로 절단하여 분석한 결과 이들은 서로 다른 그룹이라는 것을 알 수 있었다.

• 원예과학기술지
• /
• 제31권6호
• /
• pp.714-719
• /
• 2013

꽃목굽음 현상(bent peduncle phenomenon, BPP)은 절화장미의 생산성 저하를 유발하는 생리적 장해의 하나로 알려져 있으나, 관련 기작이 명확히 구명되어 있지 않아 아직까지 대책 마련이 곤란한 실정이다. 이를 해결하고자 국내육성 장미 품종인 'Beast'(Rosa hybrida L. 'Beast')를 대상으로 꽃목굽음지의 화기형태, 생장, 내생 호르몬 변화 특성을 분석하였다. 화기형태 관찰을 통해 꽃목굽음지는 5개의 꽃받침조각 중 하나가 조기에 형성되고 엽상화되면서 대화현상을 나타냈다. 연중 BPP 발생 빈도는 계절에 따라 5-20%로 여름철 고온기에 급증하였다. 꽃목굽음지는 정상지와 비교하여 꽃목굽음 증상 외 생장량과 속도면에서 차이를 보이지 않았으나, 꽃으로의 동화산물 분배는 현저하게 감소되었다. 정상지와는 달리 꽃목굽음지에서의 내생 호르몬 IAA 함량은 화아발육기간 내내 높게 유지되었으나, $GA_3$ 함량은 차이를 보이지 않았다. 본 연구에서 BPP는 고온과 관련된 특정 꽃받침조각의 엽상화, 대화현상을 나타내면서 꽃으로의 동화산물 불균형 분배, 높은 IAA에 의해 유발되는 것으로 보인다.

• The Plant Pathology Journal
• /
• 제24권3호
• /
• pp.279-282
• /
• 2008

This study describes a phytoplasmal disease occurring in Petunia leaves grown in the glasshouse of the National Horticultural Research Institute, Suwon, Korea. Abnormal growth like flattened stem with flower malformation or phyllody was observed from the plant. The DNA extracted from the diseased leaves was amplified using a universal primer pair of P1/P6 derived from the conserved 16S rRNA gene of Mollicutes giving the expected polymerase chain reaction(PCR) product of 1.5 kb. In the nested PCR assays, the expected DNA fragment of 1.1 kb was amplified with the specific primer pair R16F1/R16R1 that was designed on the basis of aster yellows(AY) phytoplasma 16S rDNA sequences. The 1.1 kb PCR products were cloned and nucleotide sequences were determined, and the sequences of the cloned 168 rRNA gene were deposited in the GenBank database under the accession no. of EU267779. Analysis of the homology percent of the 168 rDNA of PFS-K showed the closest relationship with Hydrangea phyllody phytoplasma(AY265215), Brassica napus phytoplasma(EU123466) and AY phytoplasma CHRY(AY180956). Phytoplasma isolated from the diseased Petunia was designated as Petunia flat stem phytoplasma Korean isolate(PFS-K) in this study. Flattened stem occurring in Petunia was confirmed as infection of AY group of phytoplasma by determination of 16S rRNA gene sequences of phytoplasma and microscopic observation of phytoplasma bodies. This is the first report on the phytoplasmal disease in Petunia in Korea.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.73.2-73
• /
• 2003

Typical whiches broom symptoms caused by phytoplasma were observed in Ash (Fraxinus rhynchophylla Hence) in Korea. The symptoms were showing abnormally small leaves, short internodes, and proliferation of shoots. Fluorescence and electron microscopy of leaf midribs revealed phytoplasma positive DAPI fluorescence and numerous phytoplasma bodies localized in the phloem sieve tubes. Phytoplasma DNA of 1.8 Kb was detected consistently from all symptomatic samples by the amplification of phytoplasma DNA with the phytoplasma specific primer pair Pl/P7. But no phytoplasma DNA was detected in healthy ash seedlings. Based on sequence analyses of an amplified region, this phytoplasma is closely related to Eqilodium phyllody, Mulberry dwarf, and Aster yellows phytoplasmas with the homology of 99.95 ％, 99.79 ％ and 99.78 ％, respectively, This phylogenetic analyses indicate that ash witches broom phytoplasma but is evidently distinct from the ash yellows group 16SrⅦ and should be classified into the Aster yellows group 16SrⅥ.

• 한국산림과학회지
• /
• 제94권2호통권159호
• /
• pp.103-107
• /
• 2005

Typical phytoplasma whiches' broom symptoms were observed in Ash (Fraxinus rhynchophylla Hence) in Korea. The symptoms of the disease were showing abnormally small leaves, shorted internodes and proliferation of shoots. Examination of fluorescent and electron microscopy of leaf midribs revealed numerous phytoplasma bodies localized in the phloem tube cells. The phytoplasmas were detected in all the symptomatic samples by the amplification with phytoplasma specific primer pair P1/P7 consistently, and the expected size was 1.8 kb. However, the phytoplasma DNA was not detected in healthy seedlings. Based on sequence analysis of amplified region, this phytoplasma has close homologies with eqilodium phyllody, mulberry dwarf, and aster yellow phytoplasmas, 99.95%, 99.79% and 99.78%, respectively, This phylogetic analysis indicates that ash witches' broom phytoplasma should be classified in the aster yellow group 16SrVI and clearly distinct from the ash yellow group 16SrVII.