• The Korean Journal of Zoology
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• v.36 no.1
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• pp.77-83
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• 1993

난모세포성숙기 (peripheral germinal vesicle stage)의 무지개송어(Oncorhynchus mykiss)를 대상으로 방사성 표지 전구체(3H-pregnenolone, 3H-l7$\alpha$-hydroxyprogesterone 그리고 3H-androstenedione)를 이용하여 유 nitro에서 관찰한 협막세포층의 스테로이드 주 대사 경로는 다음과 같으며, pregnenolone -) 17a-hydroxypregnenolone$\longrightarrow$ 17u-hydroxyprogesterone $\longrightarrow$ androstenedione $\longrightarrow$ testosterone 이는 $\Delta$5-스테로이드 경로의 존재가능성을 지적하고 있다.

• Journal of Nutrition and Health
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• v.41 no.3
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• pp.224-231
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• 2008

We examined the effect of indole-3-carbinol (I3C, $C_9H_9NO$), an autolysis product of a glucosinolate and a glucobrassicin in vegetables, on MMP-2, -9 activities and TIMP-l and -2 inductions via microtubule-associated protein kinase (MAPK) signaling pathway in prostate cancer cell line, PC3 cells. Our results indicated that I3C inhibited cell growth of PC3 cells in dose (0,50, 100 ,${\mu}M$) and time (0,24,48 and 72 h) dependent manners. Using gelatin zymography for MMP activity, we demonstrated that I3C significantly decrease MMP-2 and -9 activities in PC3 cells. We also observed that I3C decreased the proteins and mRNA levels of MMP-2 and -9 in PC3 cells as well. Inversely, expressions of TIMP-l and -2 protein and mRNA in PC3 cells were increased by I3C in a dose dependent manner. In another experiment, we showed that I3C inhibited PC3 cells invasiveness by using marigel invasion assay and we also found that I3C suppressed MMP transcriptional activity by MAPK signaling pathways. Taken together, our results suggest that I3C may contribute to the potential beneficial food component to prevent the cancer metastasis in prostate cancer cells. (KoreanJNutr2008; 41(3): 224~23I)