• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.2
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• pp.61-67
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• 2006

We examined seedlings of two species (Ilex rotunda and Illicium anisatum) which have a different level of shade tolerance and raised them under different light regimes (full sunlight and 50% shading). After 12 months, we investigated chlorophyll content (Chl. a, Chl. b and Chl. a+b), photosynthetic systems (photosynthetic rate, light compensation point, dark respiration rate and quantum yield), intercellular $CO_2$ concentration and water use efficiency to show acclimation reaction to different light conditions. Seedlings grown under full sunlight showed lower chlorophyll content than those in the shading regime. There was a significant difference between the full sunlight and shade treatments in I. anisatum (shade tolerance species). I. rotunda (intermediate species) showed high photosynthetic rate and water use efficiency over PPFD $1000\;{\mu}mol\;m^{-2}s^{-1}$ to full sunlight. Also, I. anisatum grown under full sunlight showed lower photosynthetic rate and water use efficiency over a range of all PPFD. This result showed that I. rotunda has a more flexible reaction system than that of I. anisatum.

• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.2
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• pp.79-89
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• 2012

This study was conducted to investigate the chlorophyll contents, photosynthetic characteristics, chlorophyll fluorescence, and growth performance of Parasenecio firmus under changing light environment. Parasenecio firmus was grown under non-treated (full sunlight) and three different shading conditions (88~93%, 65~75% and 45%~55% of full sunlight) for the experiment. Total chlorophyll content, photochemical efficiency (Fv/Fm), T/R ratio, specific leaf area (SLA), leaf area ratio (LAR), and leaf weight ratio (LWR) were increased with increasing shading level, but decreased dark respiration. Therefore, light absorption and light utilization efficiency were improved under the low intensity light. Plants under 65~75% of full sunlight had best maximum photosynthetic rate and net apparent quantum yield in May. On the other hand, the non-treated plants had lower maximum photosynthetic rate, photochemical efficiency, and chlorophyll content than the treated ones. Parasenecio firmus considered to be a sciophyte, is fairly sensitive to high intensity light. If 88-93% of full sunlight lasts for a long period, photosynthetic capacity will be sharply decreased, though limiting light. These results suggest that growth of Parasenecio firmus adapted to 65~75% of full sunlight.

• Journal of Korean Society of Forest Science
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• v.100 no.1
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• pp.88-94
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• 2011

This study was conducted to provide on a basic information for maintenance of roadside trees and identify the links between environmental pollution and tree damages (Ginkgo biloba, Platanus occidentalis) in Daejeon City by analyzing photosynthetic characteristics, water use efficiency, chlorophyll fluorescence and chlorophyll contents by the regions and plants. The investigations were conducted at Chungnam national university(CNU) considered as the least air-polluted area compared to other study sites, Daedeok science town(DS) which a new road with little traffic recently was built around, Daehwa industry complex(DIC) and Daejeon station(DS) which an old road with heavy is located around. The photosynthetic capacity of the two species were highest in CNU and lowest in the DS. However the water use efficiency was highest on the contrary to the photosynthetic capacity in DS. Chlorophyll fluorescence and chlorophyll contents were highest in CNU and lowest in DS as the photosynthetic capacity. On all of the test, Ginkgo biloba shows the more favorable physiological responses than Platanus occidentalis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.3
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• pp.296-302
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• 2013

The effects of temperature on spore release, growth and photosynthetic efficiency of Lithophyllum yessoense and Hildenbrandia rubra were examined. L. yessoense was collected at Galnam and H. rubra was collected at Gyeokpo, Korea. The experimental temperatures were different for spore release (10, 15, $20^{\circ}C$), sporeling growth (10, 15, 20, 25, $30^{\circ}C$) and photosynthetic efficiency (10, 15, 20, $25^{\circ}C$). All other culture conditions were the same: 34 psu, 12:12 LD and $50{\mu}mol$ photon $m^{-2}s^{-1}$. Spore liberation was maximal at $10^{\circ}C$ for L. yessoense and at $20^{\circ}C$ for H. rubra. After 14 days, the surface area of L. yessoense was 0.031 $mm^2$ at $25^{\circ}C$ and for H. rubra was 0.032 $mm^2$ at $20^{\circ}C$. Sporelings of L. yessoense were a dark-red color and grew in a round shape. In contrast, H. rubra was bright pink and changed from a round shape in the early growth stage to later become flabelliform. Photosynthetic efficiency was highest between $20-25^{\circ}C$ in both species. In conclusion, L. yessoense and H. rubra display different physiological features based on the optimal temperatures for spore release and sporling growth.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.143-153
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• 2019

Background: Ginseng is one of the well-known medicinal plants, exhibiting diverse medicinal effects. Its roots possess anticancer and antiaging properties and are being used in the medical systems of East Asian countries. It is grown in low-light and low-temperature conditions, and its growth is strongly inhibited at temperatures above $25^{\circ}C$. However, the molecular responses of ginseng to heat stress are currently poorly understood, especially at the protein level. Methods: We used a shotgun proteomics approach to investigate the effect of heat stress on ginseng leaves. We monitored their photosynthetic efficiency to confirm physiological responses to a high-temperature stress. Results: The results showed a reduction in photosynthetic efficiency on heat treatment ($35^{\circ}C$) starting at 48 h. Label-free quantitative proteome analysis led to the identification of 3,332 proteins, of which 847 were differentially modulated in response to heat stress. The MapMan analysis showed that the proteins with increased abundance were mainly associated with antioxidant and translation-regulating activities, whereas the proteins related to the receptor and structural-binding activities exhibited decreased abundance. Several other proteins including chaperones, G-proteins, calcium-signaling proteins, transcription factors, and transfer/carrier proteins were specifically downregulated. Conclusion: These results increase our understanding of heat stress responses in the leaves of ginseng at the protein level, for the first time providing a resource for the scientific community.

• The Plant Pathology Journal
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• v.35 no.5
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• pp.521-529
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• 2019

Tomato yellow leaf curl China virus is a species of the widespread geminiviruses. The infection of Nicotiana benthamiana by Tomato yellow leaf curl China virus (TYLCCNV) causes a reduction in photosynthetic activity, which is part of the viral symptoms. ${\beta}C1$ is a viral factor encoded by the betasatellite DNA ($DNA{\beta}$) accompanying TYLCCNV. It is a major viral pathogenicity factor of TYLCCNV. To elucidate the effect of ${\beta}C1$ on plants' photosynthesis, we measured the relative chlorophyll (Chl) content and Chl fluorescence in TY-LCCNV-infected and ${\beta}C1$ transgenic N. benthamiana plants. The results showed that Chl content is reduced in TYLCCNV A-infected, TYLCCNV A plus $DNA{\beta}$ (TYLCCNV A + ${\beta}$)-infected and ${\beta}C1$ transgenic plants. Further, changes in Chl fluorescence parameters, such as electron transport rate, $F_v/F_m$, NPQ, and qP, revealed that photosynthetic efficiency is compromised in the aforementioned N. benthamiana plants. The presense of ${\beta}C1$ aggravated the decrease of Chl content and photosynthetic efficiency during viral infection. Additionally, the real-time quantitative PCR analysis of oxygen evolving complex genes in photosystem II, such as PsbO, PsbP, PsbQ, and PsbR, showed a significant reduction of the relative expression of these genes at the late stage of TYLCCNV A + ${\beta}$ infection and at the vegetative stage of ${\beta}C1$ transgenic N. benthamiana plants. In summary, this study revealed the pathogenicity of TYLCCNV in photosynthesis and disclosed the effect of ${\beta}C1$ in exacerbating the damage in photosynthesis efficiency by TYLCCNV infection.

• Journal of Ecology and Environment
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• v.42 no.4
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• pp.174-182
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• 2018

Background: In this study, we observed their growth and physiological responses using a variety of duty ratio under the mixed light using red, blue, and white lights. The red+blue mixed light was treated with 95%, 90%, 85%, 80%, and 75% duty ratios and red+blue+white mixed light with 85% and 70% duty ratios. We examined the width and length of leaves, total number of leaves, and number of shoots to examine their growth responses. The physiological responses were studied by measuring their photosynthetic rate, transpiration rate, stomatal conductance, water use efficiency, chlorophyll content, and fluorescence ($F_o$, $F_m$, and $F_v/F_m$). Results: We found that lower duty ratio caused the length and width of the leaves to grow longer under red+blue mixed light but that it did not cause any difference in the red+blue+white mixed light condition. In addition, there was no difference in the number of leaves and shoots among all treatments. In the red+blue mixed light condition, the photosynthetic rate was no difference, but both transpiration rate and stomatal conductance were the highest at 95% duty ratio than in other ratios. Water use efficiency pattern was similar to that of photosynthetic rate; water use efficiency was no difference. Chlorophyll content was the highest at 95% duty ratios, and it was the least at 90%, 85%, and 75% duty ratio. $F_o$ and $F_m$ values were relatively high at 85% and 80% duty ratio and low at 90% duty ratio while $F_v/F_m$ showed no difference. Conclusions: Under the red+blue+white mixed light, all physiological items showed no difference between 70 and 85% treatments. But, photosynthetic rate, water use efficiency, chlorophyll content, and $F_v/F_m$ were relatively greater in the red+blue+white mixed light than in the red+blue mixed light. Therefore, red+blue+white mixed light treated with 70% duty ratio could lessen the environmental stress and save more power when cultivating Silene capitata in a plant factory.

• KSBB Journal
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• v.19 no.4
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• pp.257-262
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• 2004

Recently, there is a growing interest in microalgae and the use of microalgae focused on the production of various high value metabolite used in food, pharmaceuticals and cosmetics. The key limiting factor in high density algal cultivation is the light and algal growth is defined by light intensity and light penetration depth into the culture medium. The effect of light with various light paths, S/V ratios, light intensities, and 50% duty cycle on the growth of microalgae was examined to enhance microalgal biomass productivity and photosynthetic efficiency. We confirmed that the utilization of efficient light energy was obtained from 4 cm of diameter, 57.6% of S/V ratio, 62 ${\mu}$mol/㎡/s of light intensity.

• Asian Journal of Turfgrass Science
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• v.11 no.1
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• pp.45-58
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• 1997

Pigments and thylakoid membrane proteins were investigated in wild type and PS I- mutants from Synechocystis sp. PCC6803 Comparing morphological features, B2 was less fluorescent than the other strains. The contents of chlorophyll a were propotional to the FNR activity in thylakoid membrane. The FNR activity of mutants was lower than that of wild type. In the result of pigments analysis, mutants had smaller cholophyll a than that of wild type. The major carotenoid was found to he $\beta$-caroene, but aeaxanthin was barely detected in thylakoid membrane of mutants. The polypeptide, 14.8kD was detected by electrophoresis in mutants. It was considered to be the modification of 15.4kD in wild type. Membrane polypeptides of 17.6 and 19.7kD were not detected in mutants. In the result of western blotting, subunit I was detected in all strains, but subunit II was barely detected in mutants. Subunit II was not detected in B2 at all. In view of the results so far achieved, the changes of contents of chlorophyll and zeaxanthin were affected by the defficiency or modification of functional domain in subunit I. Also the modification in subunit I affected the subunit II- binding site in PS I. As the result, efficiency of photosynthesis was decreased. Key words: Synechoystis sp. PCC6803, PS I - mutant, Photosynthetic efficiency, Pigment,Thylakoid membrane proteins, Subunit I, II.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.1
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• pp.93-101
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• 2008

The effects of $CO_2$ enrichment on growth of maize (Zea mays L.) were examined. Parameters analyzed include growth characteristics, yields, photosynthetic rates, evaporation rates and photosynthesis-related characteristics under elevated $CO_2$. The plants were grown in growth chambers with a 12-h photoperiod and a day/night temperature of $28/21^{\circ}C$ at the seedling stage and $30/23^{\circ}C$ from the silking stage. The plants were exposed to two elevated $CO_2$ of 500, 700ppm and ambient levels (350 ppm). Chalok 1 and GCB 70 germinated three days after seeding, and germination rates were faster in the elevated $CO_2$ than the control. Germination rates displayed significant differences among the $CO_2$ treatments. At the seedling stage, leaf area, top dry weight, and photosynthetic rates, and plant height indicated positive relationship with elevated $CO_2$ concentrations. At the $5{\sim}6$ leaf stage, $CO_2$ concentration also indicated positive relationship with plant height, leaf area, top dry weight, and photosynthetic rates. At the silking stage, increased plant height of Chalok 1 was noted in the $CO_2$ treatments compared to the control. No significant differences were noted for GCB 70, in which leaf area decreased but photosynthetic rates increased progressively with $CO_2$ concentration. Stomatal aperture was a little bigger in the elevated $CO_2$ than the control. $CO_2$ concentration was negatively related to stomatal conductance and transpiration rates, resulting in high water use efficiency.