• Journal of Photoscience
• /
• 제5권1호
• /
• pp.1-10
• /
• 1998

The susceptibility of chilling-resistant spinach plants. and of chilling-sensitive squash plants to photoinhibition was compared in terms of the activity of photosystem II, in relation to the deuce of fatty acid unsaturation of chloroplast membrane lipids. From thylakoid membranes of the plants. monogalactosyl diacylgtycerol, digalactosyl diacylglycerol. sulfoquinovosyt diacylglycerol, and phosphatidylglycerol were seperated as major lipid classes. It was found that the content of cis-unsaturated fatty acids of phosphatidylglycerol was greater by 32% in spinach than that in squash. When leaf disks were exposed to light at 5$\circ$C, 15$\circ$C and 25$\circ$C, photochemical efficiency of photosystem II. measured as the ratio of the variable to the maximum fluorescence of chlorophyll, declined markedly in squash plants, as compared to spinach plants. When leaf disks were exposed to strong light in the presence of lincomycin, an inhibitor of protein synthesis in chloroplasts, photoinhibition was accelerated in the two types of plants. Moreover, lincomycin treatment abolished the differences in the degree of susceptibility to strong light, which had been observed between the two types of plants. When the extent of photoinhibition of photosystem II-mediated electron transport was compared in thylakoid membranes isolated from the two types of plants, there were no differences in the degree of inactivation of photosystem II activity. However, when intact leaf disks were exposed to strong light either at 10$\circ$C or at 25$\circ$C, and then were allowed to recover either at 17$\circ$C or at 25$\circ$C in dim light. chilling-resistant plants such as spinach and pea showed marked recovery from photoinhibition, in contrast to chilling-sensitive plants, such as squash and sweet potato. whose recovery was strongly dependent on the temperature. These findings are discussed in relation to the unsaturation of fatty acids in membrane phosphatidylglycerol. It appears that fatty acid unsaturation of membrane lipids accelerates the recovery of photosystem H from photoinhibition, without affecting the photo-induced inactivation process of photosystem II associated with photoinhibition.

• 한국광과학회:학술대회논문집
• /
• 한국광과학회 2002년도 정기총회 및 제9회 광과학 학술심포지움 논문초록
• /
• pp.58-58
• /
• 2002

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
• /
• pp.180.3-180
• /
• 2003

No Abstract, See Full Text

• 미생물학회지
• /
• 제40권3호
• /
• pp.211-216
• /
• 2004

세포외 phospholipase D (PLD)를 과량 생산하는 균주 JE-11을 토양으로부터 분리하였다. 16S rDNA에 의한 분석과 형태적, 생리적 특성을 조사한 결과 이 균은 Streptomyces somaliensis로 동정되었다. 선발한 S. somaliensis로 부터 PLD를 암호화하는 유전자(sspld) 분리하고 염기서열을 조사하였다. Open reading frame을 분석한 결과 33개의 아미노산으로 이루어진 분비 signal peptide와 505개의 아미노산으로 구성된 PLD단백질을 암호화하는 것으로 예상되었다. 또한, sspld의 염기 서열로부터 유추된 단백질 서열은 기존에 보고된 다른 Streptomyces PLD들과 70-88％의 서열 유사성을 보였다. 이 PLD는 96-98％(㏖/㏖)의 수율로서, Phosphatidylcholine을 glycerol과 serine을 기질로 하여 각각 phosphatidylglycerol 과phosphatidylserine으로 전환을 하였으나, 알코올 공여체인 inositol과 ethanolamine과는 반응하지 않았다.

• 대한전기학회논문지
• /
• 제41권8호
• /
• pp.947-950
• /
• 1992

At-cut quartz crystal has been applied as chemical vapour sensors. The responses of quartz crystal at 9 MHz coated with phosphatidylglycerol(PG), phosphatidylinositol(PI), phosphatidylethanolamine(PE), phosphatidylserine(PS), and lipid A(LA) are determined for amyl acetate, acetoin, menthone and other organic gases which showed different affinities for each lipid. The identification of odorants depending on the species of lipid used for coating is discussed in terms of the normalized resonant frequency shift pattern.

• 한국환경보건학회지
• /
• 제23권2호
• /
• pp.1-11
• /
• 1997

Copper chloride 10 ppm, manganese chloride 100 ppm, nickel chloride 50 ppm을 각각 처리한 Bacillus subtilis를 배양하는 동안에 이들 세포에서 일어나는 인지질 생합성 및 지방산 조성의 변화를 대조구와 비교하여 분석하였다. 세포의 생장과 phosphatidylethanloamine, phosphatidylcholine, phosphatidylglycerol, cardilolpin은 대조구에 비해 금속 화합물 처리구에서 저해되었는데 copper chloride가 가장 큰 억제 효과를 나타내었다. 그러나 phosphatidylinositol은 금속화합물의 영향을 받지 않았다. 인지질 생합성에 이용된 주요 지방산은 대조구에서는 palmitic acid(평균 19.00%)와 stearic acid(평균 9.58%)로 나타났다. 그러나 copper chloride 처리구는 palmitic acid(평균 17.38%)와 oleic acid(평균 15.99%)가 인지질 생합성에 이용된 주요 지방산이었고, manganese chloride 처리구는 palmitic acid(평균 15.00%)와 myristic acid(평균 14.24%), nickel chloride 처리구는 oleic acid(평균 17.87%)와 stearic acid(평균 13.78%)가 인지질 형성에 이용되었다.

• 미생물학회지
• /
• 제31권4호
• /
• pp.261-266
• /
• 1993

유일한 탄소 및 에어지원으로 메탄올만을 이용하여 성장하는 새로운 절대 메탄올 자화세균을 토양으로 부터 분리하였다. 분리균주는 그람음성의 운동성이 없고 포자를 형성하지 않는 간균으로 정대호기성 세균이었다. 이 균주는 catalase 활성과 oxidase 활성을 가지고 있으며, nitrate를 nitrite로 환원시킬 수 있고 성장시 vitamin이나 특이한 생육인자를 요구하지 않았다. 세대시간은 1.6시간으로 메탄올 동화경로로는 ribulose monophosphate pathway(Entner-Doudoroff 변형경로)를 이용하나 α-ketoglutarate dehydrogenase 활성은 없었다. Ammonium ion은 glutamate dehydrogenase를 이용하여 동화하였다. DNA의 guanine plus cytosine 함량은 61.0 mol%이고 세포내 지방산으로는 누고 straight-chain saturated C$^{16:0}$ acids(palmitie acids)와 unsaturated C$^{16:1}$ acids(palmitoleic acids)를 가지고 있으나 이외에도 두 종류의 unidentified C$_{17}$ branched fatty acid도 포함하고 있었다. Major ubiquinone은 Q-8이나 Q-6와 Q-7을 특이하게 소량 가지고 있었다. Phospatidylethanolamine과 Phosphatidylglycerol이 주요한 phospholip의 구성물질이나 diphosphatidylgycerol도 소량 포함하고 있었다. 위와같은 생리적, 생화학적 자료로 부터 분리균주를 새로은 종 즉, Methylohacillus methanolovorus sp. nov.로 명명하였다.

• KSBB Journal
• /
• 제6권3호
• /
• pp.281-288
• /
• 1991

This research was carried to investigate the effects of several organic solvents on the enzymatic transphosphatidylation in emulsion and two-phase solvent systems. The solvents having a similar dielectric constant with diethylether were effective for the enzyme activity. Diethylether and butylacetate were the most effective solvents, when added 12-15%(v/v) and 10-40%(v/v), respectively, for the synthesis of phosphatidylglycerol, phosphatidylethyleneglycol and phosphatidylpropyleneglycol. In the emulsion system, the size of ovolecithin liposome was increased and the clearness of the phospholipid bilayer was reduced as increasing the diethylether concentration. In the twophase solvent system, the rapidest reaction was obtained when water-organic solvent ratio was close to 1. The ratio of aqueous phase. however, should be lowered to 37% to gain the sole product of transphosphatidy1ation, without phosphatidohydrolysis.

• KSBB Journal
• /
• 제6권3호
• /
• pp.271-279
• /
• 1991

Phosphatidylglycerol(PG) and two unnatural phospholipids, phosphatidylethyleneglycol (PEG) and phosphatidylpropyleneglycol(PPG), were synthesized from ovolecithin using cabbage phospholipase D(PLD) in a emulsion system. Optimum pH and temperature for the enzymatic synthesis of PG, PEG and PPG in the emulsion system was 5.0-5.6 and 37$^{\circ}C$, respectively. The maximum activity for transphosphatidylation was obtained with 30-80 mM Ca++. Addition of 25% glycerol was required to convert completely ovolecithin to PG, whereas 16% glycerol was sufficient to attain the highest rate of conversion for both PEG and PPG syntheses, the highest conversion rate was obtained with addition of either 10% ethyleneglycol or propyleneglycol. However, the concentration of alcoholic acceptor should be increased up to 20% to improve selectivity up to 100% for PEG or PPG synthesis. Identification of PEG and PPG was made by analyzing the polyvalent alcohols released after their hydrolysis by HCl or PLD.

• 한국환경보건학회지
• /
• 제22권2호
• /
• pp.114-123
• /
• 1996

Escherichia coli에 copper chloride 50 ppm, manganese chloride 100 ppm, nickel chloride 100 ppm을 각각 처리하여 세포를 배양하는 동안에 이들 세포에서 일어나는 인지질 생합성 및 지방산 조성의 변화를 대조구와 비교하여 분석하였다. 세포의 생장과 total lipid, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, cardiolipin은 대조구조에 비해 금속 화합물 처리구에서 저해되었는데 nickel chloride가 가장 큰 억제 효과를 나타내었다. 그러나 phosphatidylinositol은 금속화합물의 영향을 받지 않았다. 인지질 생합성에 이용된 주요 지방산은 대조구는 palmitic acid(평균 25.47%)와 palmitoleic acid(평균 12.27%)가 인지질 생합성에 도입되었고 copper chloride 처리구는 palmitic acid(평균 30.13%)와 stearic acid(평균 9.12%)로 나타났다. manganese chloride 처리구와 nickel chloride 처리구에서는 모두 palmitic acid(평균 24.16%, 평균 21.77%)와 linoleic acid(평균 9.48%, 평균 11.88%)가 인지질 생합성에 이용된 주요 지방산으로 분석되었다.