• Food Science and Biotechnology
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• 제17권2호
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• pp.434-437
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• 2008

The aim of this study was to investigate whether Monascus-fermented soybean extracts (MFSE) containing natural estrogen-like compounds such as isoflavones and mevinolins has potential effects on human osteoblast-like SaOS2 cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and alkaline phophatase (ALP) assaies. MFSE exerted biphasic dose-dependent effect; stimulating osteoblastic activity at low concentrations and inhibiting SaOS2 cells viability at high concentrations. At $10^{-8}-10^{-4}\;mg/mL$, MFSE is not only non-cytotoxic but also induced comparatively high ALP activity on SaOS2 cells. ALP activity (%) significantly increased (220.1%, p<0.05) when SaOS2 cells were treated with MFSE at a concentration of $10^{-5}\;mg/mL$, whereas slowly increased (185.6%, p<0.05) in unfermented soybean extracts (UFSE) at $10^{-3}\;mg/mL$. The potentially greater ALP activity of MFSE compared to the UFSE might partially be caused by its mevinolin, which was derived from the soybean during Monascus-fermentation. Our findings indicate that supplementation of MFSE may accelerate the speed of intracellular ALP synthesis by the bone cells when provided at optimal dosages.

• The Korean Journal of Physiology and Pharmacology
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• 제13권3호
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• pp.201-207
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• 2009

Our previous study demonstrated that flavone inhibits vascular contractions by decreasing the phosphorylation levelof the myosin phosphatase target subunit (MYPT1). In the present study, we hypothesized that flavone attenuates vascular contractions through the inhibition of the RhoA/Rho kinase pathway. Rat aortic rings were denuded of endothelium, mounted in organ baths, and contracted with either 30 nM U46619 (a thromboxane A2 analogue) or 8.0 mM NaF 30 min after pretreatment with either flavone (100 or 300 $({\mu}M$) or vehicle. We determined the phosphorylation level of the myosin light chain ($MLC_{20}$), the myosin phophatase targeting subunit 1 (MYPT1) and the protein kinase C-potentiated inhibitory protein for heterotrimeric myosin light chain phophatase of 17-kDa (CPI17) by means of Western blot analysis. Flavone inhibited, not only vascular contractions induced by these contractors, but also the levels of $MLC_{20}$ phosphorylation. Furthermore, flavone inhibited the activation of RhoA which had been induced by either U46619 or NaF. Incubation with flavone attenuated U46619 or NaF-induced phosphorylation of $MYPT1^{Thr855}$ and $CPI17^{Thr38}$, the downstream effectors of Rho-kinase. In regards to the $Ca^{2+}$-free solution, flavone inhibited the phosphorylation of $MYPT1^{Thr855}$ and $CPI17^{Thr38}$, as well as vascular contractions induced by U 46619. These results indicate that flavone attenuates vascular contractions, at least in part, through the inhibition of the RhoA/Rho-kinase pathway.

• 대한지하수환경학회지
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• 제5권4호
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• pp.215-222
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• 1998

지하수 세균 군집에 미치는 환경요인의 영향을 분석하기 위하여, 서울 시내에서 음용수로 사용하는 2개 정점과 음용수외 생활용수로 사용하고 있는 8개 정점을 대상으로 조사하였다. 물리.화학적 환경요인과 중금속의 농도, 및 세균 군집의 분포 등 40개 변수를 분석한 결과, 음용수로 사용하는 정점을 제외한 나머지 정점에서 질산성 질소와 암모니아가 용수목적별 수질기준의 기준치 이상으로 측정되었다. 총세균은 5.1~41.4$\times$$10^{5}$cells/ml 범주로, 종속영양세균과 기능성 세균군집은 0.01~29.6$\times$$10^4$cfu/ml의 범주로 조사되었다. 세포외 효소의 활성도는 0.005~11.3$\mu$M/l/hr 의 범주로 나타났고, lipase, phophatase, $\beta$-glucosidase, cellulase, chitinase, amylase 순으로 활성도가 나타났다. 정점별로 조사된 세균 군집에 미치는 환경요인의 상호관계는 대응분석(correspondence analysis)과 다차원 척도법 분석(multidimensional scaling; MDS)으로 하였으며, 그 결과 4개의 집단으로 구분되었으며, 세균 군집에 미치는 주요한 환경요인은 정점별 잠재오염원과 일치하는 양상을 보였다.다.

• KSBB Journal
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• 제25권3호
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• pp.257-264
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• 2010

Bone morphogenetic protein-7 (BMP-7) is one of important growth factors for skeletal development and bone growth. In this work, BMP-7 was efficiently expressed in recombinant Bacillus subtilis. The mature BMP-7 protein indicated molecular weight of 15.4 kDa by Western blot assay and was secreted into culture medium with 0.35 ng/mL. The extracellular and intracellular rhBMP-7 proteins were purified by using a FPLC system with an ion exchange column and a gel filtration column. The extracellular and intracellular rhBMP-7 proteins had finally a 57.1% purity and a 36.2% purity, respectively. The purified rhBMP-7 proteins showed an intact biological activity which stimulated alkaline phophatase (ALP) activity in MC3T3-E1 cells.

• 한국토양동물학회지
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• 제5권1호
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• pp.39-46
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• 2000

The distribution and some properties of alkaline phosphatase (ALP) were investigated in the midgut of the earthworm, Eisenia andrei. The ALP activity appeared to be highly polarized toward the luminal side of epithelium, with minor ALP activity in chloragogue tissue. The epithelial and chloragogeneous tissues contained approximately 85 and 15% of total intestinal ALP activity, respectively. The optimal temperature was approximately 37$^{circ}C$ and isoelectricpoint was estimated to be 4. The treatment of neuraminidase and PtdIns-PLC failed to change the migration rate of ALPs. Also, these ALPs appeared to have a wide range of substrate specificity. The relationship between the properties and physiological significances of the midgut ALPs in Eisenia andrei was discussed.

• Pediatric Infection and Vaccine
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• 제21권1호
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• pp.29-36
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• 2014

목적: 이 연구의 목적은 면역글로불린-저항성 가와사키병의 예측 인자를 찾고 점수화된 예측 모델을 만들고자 하는 것이다. 방법: 2009년 1월부터 2012년 12월까지 세브란스 어린이 병원에서 가와사키병으로 진단된 573명의 환자를 대상으로 하였다. 실험군과 검증군으로 나누었고, 각 군들은 면역글로불린-반응성과 저항성으로 나누었다. 실험군에서 면역글로불린의 예측 인자를 찾았고, 점수화된 예측모델을 만들었다. 그리고 외적, 내적 타당성 검증을 시행하였다. 결과: 남성, 경부림프절종대, 손과 발의 변화, 혈소판, 총빌리루빈, 젖산탈수효소, CRP가 면역글로불린-저항성 가와사키병의 예측 인자로 나타났다. 점수화된 예측 모델을 만들었고, 민감도와 특이도가 실험군에서는 52.5%와 82.4%, 검증군에서는 37.8%와 81.8%로 나타났다. 결론: 우리의 점수화된 예측 모델은 한국 환자에 적용하였을 때 높은 특이도와 낮은 민감도를 갖는다.

• 동의생리병리학회지
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• 제22권6호
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• pp.1532-1536
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• 2008

Quercetin which isolated form the roots of Houttuynia cordata. was determined on the basis of IR, ID and 2D NMR specta by direct comparison with authentic compounds. Protein tyrosine phophatase 1B (PTP1B) is thought to be a negative regulator in insulin signal-transduction pathway. Insulin-resistance by the activation of PTP1B is a hallmark of both type 2 diabetes and obesity. Thus, the compound inhibiting PTP1B can improve insulin resistance and can be effective in treating type 2 diabetes and obesity. Quercetin which measured the inhibitory activity against PTP1B was 92.1% inhibition in the 30 ${\mu}g$/mL, 83.4% inhibition in the 6 ${\mu}g$/mL and 76.5% inhibition in the 3 ${\mu}g$/mL. These results suggest that quercetin retains a potential PTP1B activity.

• Journal of Nutrition and Health
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• 제29권6호
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• pp.608-614
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• 1996

This study was performed to investigate the influcence of mugwort on the duodenal ulcer induced by cysteamine administration in rats. Five groups of rats were fed each experimental diet containing 0%, 5%, 15%, 30% of mugwork powder for 10 weeks. Duodenal ulcer was induced by cysteamine injection (400mg/100g body weight) after 10 weeks of feeding experimental diets (C-0, C-5, C-15, C-30). Control animal that fed 0% mugwork powder added diet were injected saline (S-0) to compare with cysteamine injected groups. When the duodenal ulcer induced by cysteamine-HCI administration, all animals in the C-0 group formed erosion and perforating ulcer was found in 44% of animals. Higher the added mugwork ratio, more inhibited of the duodenal ulcer induced by cysteamine administration (C-5, C-15). But when the ratio of added mugwort is 30%, the inhibition effect disappeared (C-30). The alkaline phospatase activities were lower at the duodenal mucosa and small intestinal mucosa in the cysteamine treated groups(p<0.05). The acid phophatase activities were higher at the stomach, small intestine and large intestine of the cysteamine treated groups. But in mugwort added diet group, the changes of enzyme activites were lessended. The maltast activities were lower at the duodental mucosa and small intestinal mucosa of cysteamine treated groups. But in mugwort added diet group, maltase activites were recovered.

• Journal of Nutrition and Health
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• 제29권7호
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• pp.758-771
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• 1996

This study attempted to define reference data for the distribution of vitamin d status and to explore the relationship between vitamin D status and related biochemical indices in Korean women. The vitamin D status of 179 Korean women aged from 20 to 75 years was analyzed by using HPLC(High Pressure Liquid Chromatography). Related biochemical indices such as iPTH, alkaline phosphatase, creatinine, albumin, Ca, Mg and P were also measured. The mean serum level of 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D were s25.8ng/ml and 89.8pg/ml, respectively. Low 25-hydroxyvitamin D (<25nmol/L) was found in 29 subjects(16.5%). There was a significantly progressive decrease in serum 25-hydroxyvitamin D with increasing age(p<0.05). After in their, there was a dramatical reduction in 25-hydroxyvitamin D(p<0.05). It was also significant in post-menopasusal women compared with pre-menopausal women(p<0.000). Serum alkaline phosphatase levels increased significantly with age(p<0.001). Whereas serum calcium and phosphorus levels remained constant with age. Serum 250-hydroxyvitamin D was invesely related to iPTH (p<0.05) and alkaline phophatase (p<0.001).

• 한국동물학회지
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• 제35권4호
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• pp.526-533
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• 1992

포유류 배아의 착상기작을 규명하고자 본 연구에서는 착상시기에 수정된 배아가 착상부위를 어떻게 인식하는 지 그리고 착상부위의 분화유발요인이 무엇인지를 조사하였다. 착상시기(임신 제6일)와 착상후 초기배아 발생기간(임신 제9일)중 혈청내 estradiol (E2)과 Progesterone(P4)의 농도를 측정하였으며, 자궁내막 조직을 착상부위 (antimesome trium)와 비 착상부위(mesometrium)로 분리하여 P4의 수용체 농도를 측정하였다. 혈청내 E2의 농도는 임신 제6일군에서 E2처리군에서 가장 높았으며, 임신 제9일에서는 intact 대조군에서 가장 높고 제9일의 모든 실험군의 농도는 임신 제6일군보다 높았다. 혈청내 P4 농도는 임신 제6일과 9일에서 다같이 대조군에서 가장 높으며 처리군 중에서는 P4처리군에서 높아지고 있다. P4 농도 역시 임신 제9일의 모든 실험군에서 제6일의 실험군보다 높았다. P4수용체 농도는 착상부위가 비 착상부위보다 높으며, 대조군(P < 0.01)과 P처리 군(P < 0.05)에서는 유의한 차이로 비 착상부위보다 높았다. 자궁내막조직 착상을 위한 분화에는 P4의 영향이 크며 P4의 혈청내 농도와 핵의 수용체 농도는 모든 실험군에서 상응하는 관계를 나타내었다. 이 결과는 앞서 일차적으로 발표한 알카리성 phosphatase (ALPase) 활성과도 상응하는 것이다.