• 한국식품영양과학회지
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• 제27권5호
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• pp.852-857
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• 1998

Three kinds of kimchi using Chinese cabbage, leafy radish and mustard leaf were prepared by conventional method and stored at 5$^{\circ}C$ or 2$0^{\circ}C$ for 13 dyas. During storage at both temperatures, changes of the amounts of salt and ascorbic acid, pH and total acidity were determined, and the relationship of the decomposition of chlorophylls with the production of their derivaties was studied. At both storage temperatures, salt concentration of Chinese cabbage kimchi(3.7%), leafy radish kimchi(3.6%), mustard leaf kimchi(3.5%) was relatively constant during the entire storage period. However, pH and total acidity wre fluctuating with the remarkable changes during 3 days of storage. Ascorbic acid content was slowly decreased during the storage period and the decompositin rate of ascorbic and was greater at 2$0^{\circ}C$ than 5$^{\circ}C$. Among the kinds of kimchi tested, mustard leaf kimchi with the slow decomposition rate of ascorbic acid contained relatively high ascorbic acid content, while leafy radish kimchi contained the lowest content. At both storage temperatures, the production of pheophytin and pheophorbide from decomposition of chlorophyll was least in mustard leaf kimchi, but similar production rates in leafy radish and Chinese cabbage kimchi were observed.

• Journal of Plant Biotechnology
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• 제50권
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• pp.27-33
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• 2023

Zizyphus jujube is a plant in the buckthorn family (Rhamnaceae) that has been the subject of research into antibacterial, antifungal and anti-inflammatory properties of its fruit and seed. However, few studies have investigated its leaves. In this study, the anti-inflammatory activity of ZJL (an extract of Z. jujube leaf) was evaluated to verify its potential as an anti-inflammatory agent and SARS-CoV-2 medicine, using nitric oxide (NO) assay, RT-PCR, SDSPAGE, Western blotting, and UHPLC/TOFHRMS analysis. We found that ZJL suppresed pro-inflammatory mediators such as NO, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and tumor necrosis factor α (TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells. ZJL acted by inhibiting NF-KB and MAPK signaling pathway activity. We also confirmed that ZJL contains a phenol compound and flavonoids with anti-inflammatory activity such as trehalose, maleate, epigallocatechin, hyperoside, catechin, 3-O-coumaroylquinic acid, rhoifolin, gossypin, kaempferol 3-neohesperidoside, rutin, myricitrin, guaiaverin, quercitrin, quercetin, ursolic acid, and pheophorbide a. These findings suggest that ZJL may have great potential for the development of anti-inflammatory drugs and vaccines via inhibition of NF-κB and MAPK signaling in LPS-induced RAW264.7 cells.

• Molecules and Cells
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• 제40권10호
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• pp.773-786
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• 2017

The loss of green coloration via chlorophyll (Chl) degradation typically occurs during leaf senescence. To date, many Chl catabolic enzymes have been identified and shown to interact with light harvesting complex II to form a Chl degradation complex in senescing chloroplasts; this complex might metabolically channel phototoxic Chl catabolic intermediates to prevent oxidative damage to cells. The Chl catabolic enzyme 7-hydroxymethyl Chl a reductase (HCAR) converts 7-hydroxymethyl Chl a (7-HMC a) to Chl a. The rice (Oryza sativa) genome contains a single HCAR homolog (OsHCAR), but its exact role remains unknown. Here, we show that an oshcar knockout mutant exhibits persistent green leaves during both dark-induced and natural senescence, and accumulates 7-HMC a and pheophorbide a (Pheo a) in green leaf blades. Interestingly, both rice and Arabidopsis hcar mutants exhibit severe cell death at the vegetative stage; this cell death largely occurs in a light intensity-dependent manner. In addition, 7-HMC a treatment led to the generation of singlet oxygen ($^1O_2$) in Arabidopsis and rice protoplasts in the light. Under herbicide-induced oxidative stress conditions, leaf necrosis was more severe in hcar plants than in wild type, and HCAR-overexpressing plants were more tolerant to reactive oxygen species than wild type. Therefore, in addition to functioning in the conversion of 7-HMC a to Chl a in senescent leaves, HCAR may play a critical role in protecting plants from high light-induced damage by preventing the accumulation of 7-HMC a and Pheo a in developing and mature leaves at the vegetative stage.

• 한국식품영양과학회지
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• 제26권4호
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• pp.563-568
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• 1997

갓김치의 발효과정 중 chlorophylls, chlorophyll de-rivatives 및 carotenoids의 함량 변화와 갓김치에서 얻어진 이의 조획분의 항산화성을 살펴보았다. 갓김치 발효기간에 따른 chlorophylls 및 carotenoids의 함량은 발효 7일 동안 chlorophylls가 pheophytins로 급격히 전환되었으며 chlorophylls와 함께 chlorophyllides는 높은 농도는 아니지 만 15일 까지는 존재하다가 그 이후 모두 pheophytins와 pheophorbides로 변했다. 갓김치 발효기간 동안 total chlorophylls에는 큰 변화없이 거의 일정하게 유지 되었으나 carotenoids는 발효 말기인 25일째에 초기 함량의 43.7%로 감소되었다. 갓김치에서 얻어진 chlorophylls와 carotenoids 조분획이 지방질의 자동산화에 미치는 영향을 조사하였을 때 $\alpha$-tocophe-rol 보다 높은 항산화성을 나타내었으며 발효기간에 따른 갓김치의 항산화성을 비교하였을 때 유의적으로 차 이는 없었다. 갓김치의 chlorophylls와 carotenoids 분획이 지방산의 산화에 대한 저해작용을 나타내는 동안 chlorophylls와 caratenoids의 감소율을 비교했을 때 총 chlorophylls가 총 caratenoids 보다 안정성이 높았다.

• 동아시아식생활학회지
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• 제13권6호
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• pp.586-592
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• 2003

본 연구는 국내에서 시판되고 있는 몇 가지 품질의 말차를 이용하여 물의 온도와 교반 시간에 따른 크로로필 함량과 클로로필 패턴을 분석한 것으로서 그 결과를 요약하면 다음과 같다. 1. 한일 말차 각 3종류에 대한 클로로필 함량은 일본산 중품(S5 ; 8.34mg/g), 하품(S6 ; 8.23mg/g)이 가장 많았고 한국산 중품(S2 ; 4.87mg/g)이 가장 적었다. 2. 물의 온도에 대하여는 한국산 말차 중ㆍ하품(S2와 S3)을 제외하고는 85$^{\circ}C$와 95$^{\circ}C$에서 클로로필 함량이 가장 많았다. 3. 교반 시간에 따른 클로로필 함량은 일본산 말차 상ㆍ중ㆍ하(S4 ; 7.87mg/g, S5 ; 8.27mg/g, S6 ; 8.08mg/g)은 60초 교반이 가장 많은 반면 한국산 말차 상품과 하품(S1 ; 6.72mg/g, S3 ; 5.02mg/g)은 15초 교반이 많았다. 4. 클로로필 패턴에 있어서는 페오포비드, 클로로필 a, b 페오피틴 a, b의 5종류의 peak를 확인하였고, 일본산 상품 말차(S4)가 한국산 상품 말차(S1)보다 클로로필 a의 함량이 약 10% 많았다. 그러나 페오피틴 a는 S1이 S4보다 약 20% 많이 함유하고 있었다. 5. 한국산 말차 중 클로로필 패턴의 비율을 보면 상푸(S1)의 클로로필 a의 비율이 47.42%로서 가장 많은 반면 중품(S2)은 30.70%, S2는 23.20%, S3는 37.68%로서 품질간에 뚜렷한 차이를 나타내었다. 일본산 말차의 경우, 클로로필 a는 상품(S4)이 51.57%, 중품(S5)이 63.51%, 하품(S6)이 63.89%이었고 페오피틴 a는 S4, S5, S6가 각각 14.90%, 14.21%, 13,26%로서 한국산 말차에 비하여 뚜렷한 차이는 볼 수 없었다.