• Journal of Applied Biological Chemistry
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• v.58 no.4
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• pp.295-301
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• 2015

Five phenylpropanoids (1-5), a benzofuran neolignan (6), two 8-O-4'-neolignans (7-8), and five tetrahydrofuran lignans (9-13) were isolated from a methanol extract of Myristica fragrans seeds. The structures of 1-13 were determined by $^1H$- and $^{13}C$-NMR spectroscopic data analyses and a comparison with the literature data. Compound 3 was isolated for the first time from this plant. All the isolated compounds were evaluated for their inhibitory activity against tyrosinase. Among them, safrole (1) showed significant inhibitions against both the monophenolase ($IC_{50}=32.11{\mu}M$) and diphenolase ($IC_{50}=27.32{\mu}M$) activities of tyrosinase. The kinetic analysis shows that safrole (1) is competitive inhibitors for both monophenolase and diphenolase. The apparent inhibition constant ($K_i$) for safrole (1) binding with free enzyme was determined to be 16.05 and $13.66{\mu}M$ for monophenolase and diphenolase, respectively.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.193.2-194
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• 2003

Effect of phenylpropanoids on Phospholipase A2 (PLA2) and phosphodiesterase (PDE) activities in the asthmatic lung tissue were studied in guinea pigs. Bronchial asthma were introduced by the challenge of aerosolized ovalbumin (OA) in the double-chambered plethysmograph at twenty one days after sensitization of OA in guinea pigs. Bronchoalveolar lavage fluids (BALF) were taken by brochalveolar lavage with HEPES buffer. (omitted)

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.194.2-195
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• 2003

Phenylpropanoids(PP), C6-C3 compounds, are widely distributed in many plants. In this experiments, effect of PP on sheep red bood cells (sRBC)-induced delayed type hypersensitivity (DTH) were studied in ICR male mice. SRBC were challenged by i.p. injection at two weeks after sensitization of Lp. injection of sRBC. Five days after the challenge of antigen, paw edema induced 24 hours after the last challenge by DTH, respectively. (omitted)

• Bulletin of the Korean Chemical Society
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• v.33 no.1
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• pp.278-280
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• 2012

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.195.1-195.1
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• 2003

Many kinds of phenylpropanoids(PP), C6-C3 compounds, are widely distributed in many plants. In this experiments, effect of PP on sheep red bood cells (sRBC)-induced Arthus reaction (AR) were studied in ICR male mice. SRBC were challenged by i.p. injection two weeks after sensitization of Lp. injection of sRBC. Five days after the challenge of antigen, paw edema induced 3 hours after the last challenge by AR. (omitted)

• Proceedings of the PSK Conference
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• 2000.04a
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• pp.183.1-183.1
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• 2000

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.207.1-207.1
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• 2001

• Applied Biological Chemistry
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• v.47 no.3
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• pp.366-369
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• 2004

Myristica fragrans Houtt were extracted in 80% aq. MeOH and solvent fractionated sing $CHCl_3$, EtOAc, n-BuOH and water, successively. The n-BuOH fraction gave three phenylpropanoids through application of silica gel column chromatographies. The chemical structures of the phenylpropanoids were determined by the interpretation of several spectral data, including NMR and MS as meso-dihydroguaiaretic acid (1), nectandrin B (2) and syringin methyl ether (3). Compound 1, which was first isolated from this plant by authors, showed inhibitory activities with $60.0{\pm}2.1%\;(100\;{\mu}g/ml),\;42.6{\pm}0.9%\;(140\;{\mu}g/ml)\;and\;12.2{\pm}0.2%\;(200\;{\mu}g/ml)$ on ACAT(acyl-CoA:Cholesterol Acyltransferase), chitin synthase III and HMG-CoA reductase (3-hydroxy-3-methylglutaryl coenzyme A reductase), respectively. Compound 3 showed inhibitory activities with $27.2{\pm}0.9%\;(100\;{\mu}g/ml),\;45.5{\pm}0.8%\;(200\;{\mu}g/ml)$ on ACAT and chitin synthase III.

• Bulletin of the Korean Chemical Society
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• v.31 no.2
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• pp.495-496
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• 2010

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.185.2-186
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• 2001