• Journal of Microbiology and Biotechnology
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• v.6 no.2
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• pp.116-119
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• 1996

The biological treatment by a mixed culture GE2 immobilized on activated carbon was investigated with a phenolic resin industrial wastewater containing 41,000 mg/l of phenol and 2,800 mg/l of formaldehyde. At a dilution of 20 times with aerated tap water, influent and effluent $COD_{Mn}$ were 4,587 mg/l and 46 mg/l, that is, $COD_{Mn}$ removal efficiency was 99.0%. At this time, phenol and formaldehyde con-centration of the effluent were 1.24 and 6.80 mg/l, indicating removal efficiencies of 99.9 and 94.1%, respectively.

• Microbiology and Biotechnology Letters
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• v.23 no.4
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• pp.492-498
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• 1995

Net type polyvinylidene chloride (PVDC) media and cillium type polyethlene polypropylene (PEPP) media were installed in the aereted submerged biofilm reactors. Synthetic phenol wastewater for feed were made to contain 1,480 mg of phenol per liter of water. The organic loading range of reactors were 0.439-0.456 kg COD/m$_{3}$, 0.882 - 0.919 kg COD/m$_{3}$ and 1.199-1.339 kg COD/m$_{3}$. Comparing PVDC to PEPP media, the bacterial number found in biofilm on PEPP were slightly higher. With the low temperature (10$\circ$C), the number of bacteria was some what deceered. Number of bacterial strains identified from PVDC were 23 and those from PEPP were 42. Genera identified in the PVDC media were Flavobacterium (47.8%), Unidentified (17.6%), Pseudomonas (13.0%), Micrococcus (8.7%) and Beggratoa (8.7%). Genera identified in the PEPP media reactor were Pseudomonas (35.7%), Alcaligenes (19.0%), Aeromonas (14.33%) and Micrococcus (11.9%), In the steady, state, a filamentous bacteria, type 1701 was identified in all of the reactors. Paramecium sp. and fungi were present in the PVDC media reactor. While, Difflugia sp, Paramecium sp. and fungi were found in the PEPP media reactor. The low diversity of protozoa was ascribed to high concentration of phenol.

• Journal of Environmental Science International
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• v.22 no.7
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• pp.915-923
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• 2013

Decomposition of non-biodegradable contaminants such as phenol contained in water was investigated using a dielectric barrier discharge (DBD) plasma reactor in the aqueous solutions with continuous oxygen bubbling. Effects of various parameters on the removal of phenol in aqueous solution with high-voltage streamer discharge plasma are studied. In order to choose plasma gas, gas of three types (argon, air, oxygen) were investigated. After the selection of gas, effects of 1st voltage (80 ~ 220 V), oxygen flow rate (2 ~ 7 L/min), pH (3 ~ 11), and initial phenol concentration (12.5 ~ 100.0 mg/L) on phenol degradation and change of $UV_{254}$ absorbance were investigated. Absorbance of $UV_{254}$ can be used as an indirect indicator of phenol degradation and the generation and disappearance of the non-biodegradable organic compounds. Removal of phenol and COD were found to follow pseudo first-order kinetics. The removal rate constants for phenol and COD of phenol were $5.204{\times}10^{-1}min^{-1}$ and $3.26{\times}10^{-2}min^{-1}$, respectively.

• Advances in environmental research
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• v.9 no.2
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• pp.109-121
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• 2020

Phenol is frequently present as the hazardous pollutant in petrochemical and pesticide industry wastewater. Because of its high toxicity and carcinogenic potential, a proper treatment is needed to reduce the hazards of phenol carrying effluent before being discharged into the environment. Phenol biodegradation with microbial consortium offers a very promising approach now a day's. This study focused on the formulation of phenol degrading bacterial consortium with three bacterial isolates. The bacterial strains Bacillus cereus strain VCRC B540, Bacillus cereus strain BRL02-43 and Oxalobacteraceae strain CC11D were isolated from detergent contaminated soil by soil enrichment technique and was identified by 16s rDNA sequence analysis. Individual cultures were degrade 100 μl phenol in 72 hrs. The formulated bacterial consortium was very effective in degrading 250 μl of phenol at a pH 7 with in 48 hrs. The study further focused on the analysis of the products of biodegradation with Fourier Transform Infrared Spectroscopy (FT/IR) and Gas Chromatography-Mass Spectroscopy (GC-MS). The analysis showed the complete degradation of phenol and the production of Benzene di-carboxylic acid mono (2-ethylhexyl) ester and Ethane 1,2- Diethoxy- as metabolic intermediates. Biodegradation with the aid of microorganisms is a potential approach in terms of cost-effectiveness and elimination of secondary pollutions. The present study established the efficiency of bacterial consortium to degrade phenol. Optimization of biodegradation conditions and construction of a bioreactor can be further exploited for large scale industrial applications.

• KSBB Journal
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• v.14 no.2
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• pp.153-159
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• 1999

Fifteen microbes have been isolated from Jangja pond in Kuri-Si, Kyeonggi-Do. Among them, two strains showed excellent COD removal from wastewater, which were named Pseudomonas sp. BLP2052 and Flavobacterium sp. BLP20515, respectively. Optimal pH and temperature for the cell growth were 7.0 and $30^{\circ}C$ for both strains. Pseudomonas sp. BLP2052 and Flavobacterium sp. BLP20515 was applied to the reactor to treat wastewater and 66.0% and 65.7% COD (chemical oxygen demand) removal was achieved, respectively. Comparing these results to the case of applying mixed microbes present in Jangja pond, COD removal rate was 15% less. But when adding the selected microbes to the wastewater containing mixed microbes, COD removal rate increased by 5%. After 84 hour operation, we achieved 85.6% COD removal. When inhibitors were added less than 100 ppm, during the microbial wastewater treatment, Fe, Zn, Al, phenol and Cr influenced microbial activity more deterioratively in order. In the case of over 300 pm, Cr, Fe, Zn, Al and phenol showed severe deteriorative effect in order.

• KSBB Journal
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• v.22 no.4
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• pp.244-248
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• 2007

The treatment of a model wastewater containing high concentration, 10 $g/{\ell}$, of phenol in an integrated wet oxidation-aerobic biological treatment was investigated. Partial wet oxidation under mild operating conditions was capable of converting the original phenol to biodegradable organic acids such as maleic acid, formic acid and acetic acid, the solution of which was subjected to the subsequent aerobic biological treatment. The wet oxidation was carried out at 150$^{\circ}C$ and 200$^{\circ}C$ and the initial pH of 1 to 12. The high temperature of 200$^{\circ}C$ and the acidic initial condition in the wet oxidation led to effluents of which biodegradability was higher in the subsequent biological oxidation process, as assessed by chemical oxygen demand (COD) removal. Homogeneous catalyst of $CuSO_4$ was also used for increasing the oxidation rate in the wet oxidation at 150$^{\circ}C$ and initial pH of 3.0. However, the pretreatment with the catalytic wet oxidation resulted in effluents which were less biodegradable in the aerobic biological process compared to those out of the non-catalytic wet oxidation at the same operating conditions.

• Journal of Environmental Science International
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• v.10 no.2
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• pp.99-103
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• 2001

Several microorganisms which degrade phenol and trichloroethylene(TCE) were isolated from the activated sludge of a wastewater treatment plant. Among them, one isolate EL-04J showed the highest degradability and was identified as a Pseudomonas species according to morphological, cultural and biochemical properties. The phenol-induced cells of Pseudomonas EL-04J, which were preincubated in the mineral salts medium containing phenol as a sole carbon source, degraded 90% of 25$\mu$M TCE within 20h. This strain could also utilize some of methylated phenol derivatives (o-cresol, m-cresol and p-cresol) as the sole source of carbon and energy. Cresol-induced cells of Pseudomonas EL-04J also cometabolized TCE.

• Analytical Science and Technology
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• v.12 no.2
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• pp.141-150
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• 1999

Twenty organic chemical substances, tetrachloroethylene, toluene, ethylbenzene, p-xylene, m-xylene, isopropyl benzene, stylene, bromobenzene, 1,3,5-trimethylbenzene, 2-chlorotoluene 1,2,4-trimethylbenzene, p-isopropyltoluene, 4-chlorotoluene, n-butylbenzene, 1,2,4-trichlorobenzene, naphthalene, tert-butylbenzene, sec-butylbenzene, phenol, isopropyl benzene hydroperoxide were isolated from untreated and treated wastewater collected at 76 companys of 9 types industry in the basin of Young San River. Their organic compounds were elucidated by Gas Chromatography/Mass Spectrometry (GC/MS) and by comparison with each standard reagents. Especially, phenol compound is detected from effluent water but not detected from plant wastewater in the chemical industry. Heavy metal, which are Cr, Mn, Cu, Zn, Cd, Pb, As, Al and Fe, are contained in the plant wastewater of all industry, Fe, Al of them is more detected than the other metals in plant wastewater. Cr, Cd, Pb, As is contained much in plant wastewater of electricity and electron, metal molding industry. Nine metals is nearely treated when plant wastewater is treated, and then the concentration of each other metals is detected below water quality standard or not detected by using AA.

• Journal of Environmental Health Sciences
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• v.38 no.3
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• pp.251-260
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• 2012

Objectives: The purpose of this study was evaluating the applicability of the circulation dielectric barrier plasma process (DBD) for efficiently treating non-biodegradable wastewater, such as phenol. Methods: The DBD plasma reactor system in this study consisted of a plasma reactor (discharge, ground electrode and quartz dielectric tube, external tube), high voltage source, air supply and reservoir. Effects of the operating parameters on the degradation of phenol and $UV_{254}$ absorbance such as first voltage (60-180 V), oxygen supply rate (0.5-3 l/min), liquid circulation rate (1.5-7 l/min), pH (3.02-11.06) and initial phenol concentration (12.5-100 mg/l) were investigated. Results: Experimental results showed that optimum first voltage, oxygen supply rate, and liquid circulation rate on phenol degradation were 160 V, 1 l/min, and 4.5 l/min, respectively. The removal efficiency of phenol increased with the increase in the initial pH of the phenol solution. To obtain a removal efficiency of phenol and COD of phenol of over 97% (initial phenol concentration, 50.0 mg/l), 15 min and 180 minutes was needed, respectively. Conclusions: It was considered that the absorbance of $UV_{254}$ for phenol degradation can be used as an indirect indicator of change in non-biodegradable organic compounds. Mineralization of the phenol solution may take a relatively longer time than that required for phenol degradation.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.2 no.1
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• pp.19-31
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• 1982

The purpose of the research is to investigate the applicability of the filter activated sludge process for the treatment of toxic phenolic wastewaters. The experiment for the research was carried out by continuously feeding synthetic phenol wastewater for four periods, and the results show that a synthetic fiber filter is an adequate material for filter activated sludge process when taking consideration of durability, SS removal efficiency and wastewater permeability. The permeability of the filter sharply decreases when the temperature of the reactor is below $10{\sim}15^{\circ}C$ for a long period. In filter activated sludge process, even under high volumetric loading conditions, high phenol removal efficiencies can be attained due to the high microbial sludge concentration in the reactor and consequently low F/M ratio. In this research, the effluent phenol concentration were checked to be below $0.1mg/{\ell}$ at the influent phenol concentrations of $63{\sim}468mg/{\ell}$. During the research very low microbial yield coefficients, 0.035~0.160 kg SS/kg COD removed, were observed and the temperature coefficient for aerobic sludge digestion was measured to be 1.021.