• Journal of Microbiology and Biotechnology
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• 제20권5호
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• pp.881-888
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• 2010

solvent-tolerant bacterium strain, MH6, was isolated by hydrophilic organic solvent DMSO enrichment in the medium and identified as Serratia marcescens. The extracellular protease with novel organic-solvent-stable properties from strain MH6 was purified and characterized. The molecular mass of the purified protease was estimated to be 52 kDa on SDS-PAGE. The open reading frame (ORF) of the MH6 protease encoded 504 amino acids with 471 amino acid residues in the mature protease. Based on the inhibitory effects of EDTA and 1,10-phenathroline, the MH6 protease was characterized as a metalloproteinase. The enzyme activity was increased in the presence of $Ni^{2+}$, $Mg^{2+}$, and $Ca^{2+}$. The protease could also be activated by the nonionic surfactants Tween 80 (1.0%) and Triton X-100 (1.0%). The protease showed remarkable solvent stability in the presence of 50% (v/v) solutions of long-chain alkanes and long-chain alcohols. It was also fairly stable in the presence of 25% solutions of hydrophilic organic solvents. Owing to its high stability in solvents and surfactants, the MH6 protease is an ideal candidate for applications in organic catalysis and other related fields.

• Archives of Pharmacal Research
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• 제24권4호
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• pp.355-359
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• 2001

A novel HPLC method with electrochemical detection has been developed for the determination of recombinant human epidermal growth factor (rhEGF) in pharmaceutical products. rhEGF was separated from other components in formulation on a reversed-phase C18 column with 24% acetonitrile in 0.1 M phosphate buffer (pH 4.75). The optimum electrochemical oxidation of EGF was obtained at 0.85 V vs. Ag/AgCl in a glassy carbon working electrode due to electroactive tyrosine, tryptophan, methionine, and arginine residues. The quantitation range was from 1.0 to 200 ng of rhEGF with the linear correlation coefficient greater than 0.999. The method was successfully applied for the quantitation of rhEGF in a pharmaceutical preparation.

• 한국환경농학회지
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• 제36권3호
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• pp.184-192
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• 2017

BACKGROUND: This study was performed to evaluate the residue dissipation of boscalid and spinetoram on crown daisy and sweet pepper affected by the morphology of the crop. The half-lives and dissipation rate constants for boscalid and spinetoram on crown daisy and sweet pepper were calculated. And then lower limit of 95% confidence interval for dissipation rate constant could be used to propose the pre-harvest residue limit. METHODS AND RESULTS: The pesticide products diluted according to the pesticide label were applied one time on crown daisy and sweet pepper at 2 field sites, respectively. Initial concentration of boscalid and spinetoram on crown daisy after application were in the range of 72.80~117.15 mg/kg and 2.82~4.67 mg/kg, respectively. And Initial concentration of boscalid and spinetoram on sweet pepper were in the range of 1.58~1.62 mg/kg and 0.10~0.21 mg/kg, respectively. Boscalid and spinetoram for crown daisy dissipted below the maximum residue limit(MRL) at 10 and 2 days after application, respectively. All residues concentration of boscalid and spinetoram for sweet pepper below the MRL at 0 day after application. The half-lives based on dissipation rate constant for boscalid and spinetoram on crown daisy were 4.2~4.9 days and 3.0~2.4 days respectively. And the half-lives for boscalid and spinetoram on sweet pepper were 6.7~7.0 days and 2.8~4.0 days respectively. CONCLUSION: The difference in initial concentration of boscalid and spinetoram among crop commodities were due to different crop morphology with larger surface areas. This study was suggested that pre-harvest residue limit would be calculated from lower limit of 95% confidence interval for dissipation rate constant and would be useful to protect consumers by controlling the pesticide residues in crop.

• 한국균학회지
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• 제19권1호
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• pp.79-84
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• 1991

영지의 유효 성분을 공업적으로 대량 생산하기 위해서는 탄소원으로 인삼박을 질소원으로 corn steep liquor 를 배지로 사용하는 것이 좋았으며, 이때의 균사체 건조 중량은 1.25g/100 ml로 탄소원으로 포도당, 질소원으로 펩톤을 사용한 대조군보다 2.5배가 높았다. 균사체의 추출은 발효액을 그대로 $121^{\circ}C$(kg/cm2)에서 2시간 정도 추출하는 것이 적당하였으며, 이런 조건으로 추출하였을 때 발효액의 단백다당류의 수율은 0.75%로 동일 조건하에서 자실체의 수율인 2.6%의 0.29배였다. 균사체에서 얻어진 단백다당류의 당함량은 38.1%로 자실체에서 얻어진 것의 당함량인 14.8%의 2.6배였다. 영지에서 추출된 단백다당류로 고혈압 쥐를 이용한 혈압 강하 실험에서 자실체의 경우 최대 혈압 강하치는 26.3 mmHg였으며, 균사체의 경우는 31.6 mmHg로 균사체가 자실체보다 혈압 강하 효과가 우수하고 지속적이었다. 자실체 1g 에 상당하는 발효액의 양은 단백 다당류의 수율을 기준으로 하면 3.5ml였고 , 단백 다당류의 수율과 당함량을 고려할 경우는 1.4 ml였다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.219.2-220
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• 2003

Effect of lipids on the oxidative inactivation of paraoxonasel (PON1), a HDL -associated antioxidant protein, was investigated, based on the hydrolysis of phenyl acetate. First. various fatty acids were examined for the prevention against the inactivation of PON1 byascorbate/Cu2+ system, which caused a remarkable ($\geq$ 90％) inactivation of PON1, accompanied by a partial loss of histidine residues. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.179-180
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• 2002

The phospholipase $A_2$($PLA_2$) family represents a diverse group of enzymes that hydrolyze sn-2 fatty acid from the cell membrane. Several mammalian cytosolic $PLA_2$ and secretory $PLA_2$(s$PLA_2$) have been characterized and classified into different families. At present, 12 distinct sPLA$_2$s have been identified in mammals and classified into different groups, depending on their primary structures as characterized by the number and position of cysteine residues. (omitted)

• 약학회지
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• 제43권6호
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• pp.776-781
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• 1999

Dockings of 4,5-diarylpyrroles into cyclooxygenase-1 and cyclooxygenase-2 were carried out by GOLD program. The sulfonyl groups bonded to 5-phenyl ring of 4,5-diarylpyrroles are directed to Arg513 of COX-2 and Tyr385 of COX-2 docking modes of pyrroles are different from COX-1. Tyr385 and Arg120 of COX-1 and COX-2 have been recognized as important residues. Val523 of COX-2 may be also important. A new COX-2 selective inhibitors could be designed from the docking study.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.134.2-135
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• 2003

We examined the minimal amino acid sequence of bovine lactoferricin (Lfcin-B), a cationic peptide corresponding to residues 17-41 near the N-terminus of bovine lactoferrin, to induce apoptosis in THP-l human monocytic leukemic cells using synthetic peptides. A synthetic peptide (Lfc-17/29, amino acid sequence; FKCRRWQWRMKKL) which is consist of 13 amino acids near the N-terminus of Lfcin-B induced cell death in THP-1 cells in a dose-dependent manner, showing apparent apoptotic changes such as hypodiploid forms of genomic DNA and apoptotic DNA fragmentation. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.152.2-152.2
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• 2002

• 한국자기공명학회논문지
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• 제27권4호
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• pp.23-27
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• 2023

Hoxc, or the Homeobox C cluster, is a group of genes that play a crucial role in embryonic development, particularly in patterning the body along the anterior-posterior axis. These genes encode transcription factors, which are proteins that bind to DNA and regulate the expression of other genes. Hoxc9 is specifically involved in the development of the skeletal system, nervous system, and adipose tissue. Hoxc9 overexpression has been linked to the development of various cancers such as leukemia and breast cancer. Here, we assigned the chemical shifts Hoxc9 DNA binding domain (DBD) using heteronuclear NMR techniques. The helical regions of Hoxc9 DBD correspond to the residues T200 - F213 (Helix I), T218 - L229 (Helix II), and T232 - K249 (Helix III). Our result would be helpful for studing the molecular interactions of the Hoxc9 DBD and other proteins.