• 생명과학회지
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• 제20권6호
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• pp.934-939
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• 2010

박테리오신의 항균활성 개선을 위해 선행연구에서 개발된 ADH 프로모터에 의해 박테리오신 유전자를 발현시키는 효모발현 벡터에 GPD 프로모터 단편을 도입하여 강력한 프로모터를 가진 효모발현 재조합 플라스미드DNA를 제작하였다. ADH 프로모터에 의해 박테리오신 유전자를 발현시키는 기존의 형질전환 효모들에 비해 새롭게 개발된 GPD 프로모터에 의해 박테리오신 유전자를 발현시키는 형질전환 효모들이 그람양성 대표세균인 고초균(B. subtilis)과 그람음성 장내세균인 대장균(E. coli) 모두에서 보다 높은 생육억제환을 나타내는 것을 확인 하였다. 이 연구의 결과로 GPD 프로모터에 의해 발현이 유도되는 박테리오신 생산 효모들을 이용하여 부패하기 쉬운 식품들의 보존성을 향상시키기 위한 보존제 대체물질 또는 가축 사료에서 병원균의 생육을 저해하기 위한 항생제 대체물질의 산업적인 생산이 가능하게 될 것으로 기대된다.

• 노동경제논집
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• 제29권2호
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• pp.117-143
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• 2006

본 논문은 1963년부터 2003년까지의 40년간의 국내의 금속, 화학, 금융, 자동차 운수부문(sector)의 조합원 수가 시기별로 어떤 증가나 감소 패턴을 보이는가를 규명한다. 또한 이 4개 부문에 속한 산업(industry)인 금속부문의 자동차 조립, 자동차 부품, 조선산업, 화학부문의 시멘트, 석유정제, 제약산업, 금융부문의 민간은행업, 그리고 자동차 운수부문의 시내버스업에서 노조 조직률을 추정하여 산업별로 상대적 노조규모의 차이를 보여준다. 이러한 노조규모의 절대적인 혹은 상대적인 규모의 시기별 변화나 산업별 차이가 각 부문이나 산업이 처한 경제적 환경(성장 단계나 경로)과 제도적 환경(정부개입의 유형이나 정도) 및 사회적 환경(근로자들의 학력, 성별, 연령 혹은 회사의 규모 등의 산업구조)에 영향을 받는 사용자들이나 노조의 태도나 선택에 의해 설명된다. 부문이나 산업수준의 다양성을 고려한 본 논문의 미시적인 사회 경제적 이론들은 선진국의 국제비교 노사관계의 최근 연구들에서 나타나는 미시적인 연구의 필요성의 증가와 맥을 같이한다.

• 문화기술의 융합
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• 제6권3호
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• pp.145-158
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• 2020

인도 뭄바이에 위치한 다라비(Dharavi)는 70만 명이 넘는 수의 사람들이 한 지역에 밀집하여 살아가는 지상 최대의 슬럼가이다. 주정부는 다라비의 재개발을 함께 추진할 민간 사업자를 선정하고 계획을 진행하고 있다. 게다가 인도정부는 2015년 3가지 도시개발 이니셔티브를 발표하고 인도 전국적 차원의 도시개발과 기존 도시의 재개발사업 및 빈민가 주택공급 사업 등을 병행하여 추진하고 있다. 본 논문에서는 인도의 DMIC 산업회랑과 스마트 도시건설 등의 현행 사업들을 인도가 타산업 대비 비교우위를 가진 제약산업과의 연계를 통하여, 다리비 내의 특정한 대지에 거주하는 기존주민들을 대상으로 신규 주택의 공급이 가능한 도심형 제약연구&생산시설 및 주거복합 계획안을 제안하여 재개발 방향들 중 가능한 하나의 프로토타입(prototype)으로 제시하고자 한다.

• Journal of Pharmaceutical Investigation
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• 제22권3호
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• pp.205-210
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• 1992

In order to develop a controlled-release oral drug delivery system (DDS) of theophylline (TP), microporous membrane-coated tablets were prepared and evaluated in vitro and in vivo. Rapidly water-soluble core tablets of TP (300 mg) were prepared by wet granulation and compression technique, Then the core tablets were spray-coated with polyvinylchloride (PVC) in which micronized sucrose particles were dispersed. Effect of formula compositions of coating suspensions on the pharmaceutical characteristics such as membrane strength and dissolution was investigated in vitro. The membranes remained unbroken in pH 1.2 buffer at $37^{\circ}C$ at least for 2 hours after the disintergration test. TP was released from the coated-released tablets at a zero-order rate over 8 hours. The release at pH 1.2 and 4.0 was similar in rate but a little more rapid than that at pH 6.8. The coated tablets were administered to three healthy male volunteers and their saliva profiles of TP were compared with those from the commercial sustained release TP tablets such as Slobid and Asconthin. Saliva TP concentrations from the coated tablets were successfully sustained over 48 hours after the dosing and were comparable to those of the commercial sustained-release tablets. The membrane-coating technique is very simple and does not need any sophisticated equipments. In this respect, the membrane-coated tablets may be superior to the commercial sustained-release tablets and this technique is worth adopting by the pharmaceutical industries.

• The Korean Journal of Pain
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• 제35권1호
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• pp.33-42
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• 2022

Background: Cupressus arizonica Greene is a coniferous tree with great importance in fragrance and pharmaceutical industries. Essential oils from C. arizonica (EC) have shown potential antioxidant, and anti-microbial activities. This study aimed at investigating the anti-nociceptive and anti-inflammatory effects/mechanisms of EC. Methods: The EC was evaluated for anti-nociceptive and anti-inflammatory activities on male Wistar rats using a formalin test and carrageenan-induced paw edema, respectively. Also, we pre-treated some of the animals with naloxone and flumazenil in the formalin test to find out the possible contributions of opioid and benzodiazepine receptors to EC anti-nociceptive effects. Finally, gas chromatography/mass spectrometry (GC/MS) analysis was used to identify the EC's constituents. Results: EC in intraperitoneal doses of 0.5 and 1 g/kg significantly decrease the nociceptive responses in both early and late phases of the formalin test. From a mechanistic point of view, flumazenil administration 20 minutes before the most effective dose of EC (1 g/kg) showed a meaningful reduction in the associated anti-nociceptive responses during the early and late phases of the formalin test. Naloxone also reduced the anti-nociceptive role of EC in the late phase. Furthermore, EC at the doses of 1, 0.5, and 0.25 g/kg significantly reduced paw edema from 0.5 hours after carrageenan injection to 4 hours. GC/MS analysis showed that isolated EC is a monoterpene-rich oil with the major presence of α-pinene (71.92%), myrcene (6.37%), δ-3-carene (4.68%), β-pinene (3.71%), and limonene (3.34%). Conclusions: EC showed potent anti-nociceptive and anti-inflammatory activities with the relative involvement of opioid and benzodiazepine receptors.

• 한국정밀공학회지
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• 제31권10호
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• pp.905-911
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• 2014

A Loss-in-Weight (LIW) feeder, a type of automated measuring device, is a continuous feeder used in many mass production industries. Due to its versatility, there have been constant demands of LIW feeders in food production supply lines as well as chemical and pharmaceutical industries. In this paper, the process of designing a LIW feeder system with better performance will be examined and compared with commercial products. This system is characterized by low pass Butterworth filter and feed forward PI control. The filter is for noise disposal caused by dynamic condition of a LIW feeder. The feed forward PI control, based on linearity feature of feeders, is adequate for stable driving of the system. At the end, a possible evaluation method of LIW system will be proposed to verify the specific achievement of this paper.

• Fisheries and Aquatic Sciences
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• 제25권7호
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• pp.357-379
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• 2022

Increased fisheries products have raised by-products that are discarded due to low economic value. In addition, marine by-products are still rich in protein and nutritional value that have biological activities and give benefits to human health. Meanwhile, there is raised pressure for sustainability practices in marine industries to reduce waste and minimize the detrimental effect on the environment. Thus, valorization by-products through bioactive peptide mining are crucial. This review focus on various ways to obtain bioactive peptides from marine by-products through protein hydrolysis, for instance chemical hydrolysis (acid and based), biochemical hydrolysis (autolysis and enzymatic hydrolysis), microbial fermentation, and subcritical water hydrolysis. Nevertheless, these processes have benefits and drawbacks which need to be considered. This review also addresses various biological activities that are favorable in pharmaceutical industries, including antioxidant, antihypertensive, anticancer, anti-obesity, and other beneficial bioactivities. In addition, some potential marine resources of Indonesia for the marine biopeptide from their by-product or undesired marine commodities would be addressed as well.

• Mycobiology
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• 제51권4호
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• pp.239-245
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• 2023

Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the 𝛽-tubulin gene, isolate SP10 was identified as Penicillium menonorum. The xylanase characteristics from P. menonorum SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 ℃ while reaching 61% activity at 65 ℃. These results proposed P. menonorum SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions.

• 약학회지
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• 제59권1호
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• pp.29-39
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• 2015

In recent years, physiologically based pharmacokinetic (PBPK) modeling has been widely used in pharmaceutical industries as well as regulatory health authorities for drug discovery and development. Several application areas of PBPK have been introduced so far including drug-drug interaction prediction, transporter-mediated interaction prediction, and pediatric PK prediction. The purpose of this review is to introduce PBPK and illustrates one of its application areas, particularly pediatric PK prediction by utilizing existing adult PK data and in vitro data. The evaluation of the initial PBPK for adult was done by comparing with experimental PK profiles and the scaling from adult to pediatric was conducted using age-related changes in size such as tissue compartments, and protein binding etc. Sotalol and lorazepam were selected in this review as model drugs for this purpose and were re-evaluated using the PBPK models by GastroPlus$^{(R)}$. The challenges and strategies of PBPK models using adult PK data as well as appropriate in vitro assay data for extrapolating pediatric PK at various ages were also discussed in this paper.

• Journal of Ginseng Research
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• 제40권2호
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• pp.121-126
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• 2016

Background: Ginsenoside F1, a pharmaceutical component of ginseng, is known to have antiaging, antioxidant, anticancer, and keratinocyte protective effects. However, the usage of ginsenoside F1 is restricted owing to the small amount found in Korean ginseng. Methods: To enhance the production of ginsenoside F1 as a 10 g unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the commercial enzyme Cellulase KN from Aspergillus niger with food grade, which has ginsenoside-transforming ability. The proposed optimum reaction conditions of Cellulase KN were pH 5.0 and $50^{\circ}C$. Results: Cellulase KN could effectively transform the ginsenosides Re and Rg1 into F1. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 5.0 and $50^{\circ}C$ for 48 h with protopanaxatriol-type ginsenoside mixture (at a concentration of 10 mg/mL) from ginseng roots. Finally, 13.0 g of F1 was produced from 50 g of protopanaxatriol-type ginsenoside mixture with $91.5{\pm}1.1%$ chromatographic purity. Conclusion: The results suggest that this enzymatic method could be exploited usefully for the preparation of ginsenoside F1 to be used in cosmetic, functional food, and pharmaceutical industries.