• Proceedings of the KAIS Fall Conference
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• 2009.05a
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• pp.596-598
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• 2009

현재 상용화되어 있는 petri dish는 미생물 배지의 보관기간이 $4^{\circ}C$의 온도에서 15~50일 정도 되기 때문에 장기저장이 어렵다. 그래서 본 연구는 자체 고안된 LOP(Lab on a plate)와 petri dish에 고체배지를 제조하여 배지의 수분함량을 비교하기 위한 연구를 수행하였다. 본 연구에 사용한 배지는 Bacillus cereus 배양을 위한 MYP(Mannitol Egg Yolk Polymyxin) agar로 이용하여 제조하였으며 각각 진공포장 및 비포장하여 $4^{\circ}C$와 $25^{\circ}C$의 온도조건에서 6주 동안 매주 수분함량을 측정한 결과, LOP와 petri dish 모두 비포장 조건보다 진공포장조건의 수분보호능이 우수 하였으며 진공포장조건에서는 $25^{\circ}C$가 $4^{\circ}C$보다 상대적 수분 보호능이 좋음을 확인할 수 있었다. 또한, 비포장조건에서도 LOP가 $4^{\circ}C$와 $25^{\circ}C$ 모두 petri dish보다 수분보호능이 탁월하여 유통기간이 훨씬 더 연장될 수 있는 가능성을 보여 주어 앞으로 LOP를 이용한 장기 보관과 즉시 사용이 가능한 pre-plated media와 관련된 다양한 제품의 용기로 사용 가능할 것으로 기대된다.

• KSBB Journal
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• v.7 no.4
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• pp.278-283
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• 1992

Rat hepatocytes were isolated and cultured on the petri dishes treated with various coating materials. Untreated or collagen coated petri dish gave monolayer culture of hepatocyte and proteoglycan, dermatan sulfate, and BSA treated petri dish gave hemispheroid. The untreated Primaria petri dish gave spheroid type of hepatocyte, and heparin and hyaluronic acid treatment gave multilayers. To sustain high cell viability, monolayer cultured hepatocytes was more useful, while it was found that the hemispheroid or spheroid type hepatocytes was more active in the hepatic functions such as ammonia metabolism and albumin synthesis.

• Korean Journal of Agricultural Science
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• v.9 no.1
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• pp.223-230
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• 1982

To obtain the information on the germination and initial growth of the sesame, the seed of 6 sesame cultivars obtained from upper and lower position of plants were seeded and the results on the germination and initial growth were summarized as follows; 1. When the seeding time was delayed, the days to flower of all sesame cultivars were significantly decreased. And the shortening rate of days to flower by delaying seeding time showed significant differences among the sesame cultivars. 2. At all cultivars excluding IS 103, the germination percentage of the seeds obtained from lower position of sesame plant was significantly higher than those from upper position. 3. Germination percentage of IS 103 seeds from lower position of sesame plant was 20.0% at petri dish experiment and 75.0% at pot experiment and those from upper position of the stem was 17.0% at petri dish experiment and 72.5% at pot experiment, respectively. 4. The germination speed of all cultivars showed a same tendency as germination percentage, but the germination speed of cultivars at pot experiment was higher than those at petri dish experiment. 5. The sesame seedlings from lower position seed were increased in stem length, root length, leaf length and fresh weight than those from upper position seed. The germination percentage and the initial growth showed significant differences by the seed weight in same cultivar, however did not show any varietal differences.

• Proceedings of the KAIS Fall Conference
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• 2008.11a
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• pp.465-468
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• 2008

현재 가장 범용적으로 사용되는 미생물 배양 용기인 Petri Dish는 배지 제조 후 냉장과 실온 보관 시수분 증발에 대한 보호 효과가 적어 세 달 이상 보관하기 어려운 단점이 있어 즉시 사용 배지(pre-plated media) 개발에 장애가 되고 있다. 본 연구는 미생물 배지의 장기 저장이 가능하도록 고안된 LOP plate의 수분 보호능에 관한 것으로, LB 배지를 표준 배지로 해서 4, 25, 32 및 $37^{\circ}C$ 네 개의 온도영역과 진공 포장 및 비 포장 조건에서 Petri Dish와 비교한 결과, 각 온도 조건별로 LOP의 탁월한 수분 보호능을 확인 할 수 있었다. 따라서 향후 LOP를 이용한 장기 저장과 즉시 사용이 가능한 pre-plated media와 관련된 제품 개발이 가능할 것으로 기대된다.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.334-334
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• 2017

Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.53-53
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• 2017

Unstable soil moisture conditions often negatively affect the emergence, seedling establishment, and growth uniformity at the initial stage, and then reduce the grain yield and biomass in direct seeding cultivation for rice in rainfed fields. Therefore it is important to develop a technique to increase the rapid and stabilized seedling establishment and improve the uniformity of initial growth after sowing. This study aims to confirm results on seed priming effect of indoor using petri dish experiments can be reproduced in the field using container at Ghana. Twenty-seven rice varieties including of Oryza sativa L. and O. glaberrima Steud. were used in this study. The experiments using petri dish and container with different soil moisture conditions (5%, 10%, 15%, 20%) were compared. As a result, a significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and emergence time uniformity in the primed seed of container in 10% and 15% soil moisture condition. A significant positive correlation was found between the germination time uniformity in the primed seed of petri dish and plant height in the primed seed of container in 10% soil moisture condition in O. glaberrima. This study concluded that the priming effect in petri dish demonstrate those in container in the field condition of Ghana in 10% and 15% soil moisture condition during seedling stage.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.286-286
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• 2004

본 연구는 체외배양 system에서 체외성숙시 경제적인 수정란 생산체계를 검토하고자 체외성숙 배양 용기와 체외수정란생산 계절에 따른 효율을 비교조사하였다. 우선 저가의 국산 Petri dish (35×12 ㎜; SPL, Korea)와 고가의 4-well dish (Nunc, Denmark)를 체외성숙 용기로 사용하였다. 4-well dish 처리구에는 well 당 500 ㎍ 체외성숙 배양액에 50개의 난자를 배양하였고, Petri dish 처리구에서는 3 ㎖의 체외성숙 배양액에 300개 정도의 oocytes를 넣어 24시간 체외성숙을 유도하였다. (중략)

• Journal of Mushroom
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• v.2 no.1
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• pp.15-20
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• 2004

Pleurotus ostreatus, the oyster mushroom, is one of the most widely cultivated and important edible mushrooms in the world. In order to study the developmental process of P. ostreatus and its regulatory mechanism, a new culturing method needs to be established for inducing the fruiting body and sporulation in the laboratory. In this study, we have examined whether the fruiting body of P. ostreatus can be formed on the plastic petri dish which are commonly used for cell culture in the laboratory. The strain was cultured on $60{\times}15mm$ plastic petri dish with potato dextrose agar media at $28^{\circ}C$ for mycelial growth and then at $18^{\circ}C$ for the formation of primordia and fruiting bodies within plant growth chamber. The development of primordia into fruiting bodies was achieved on cultured dishes under air ventilation. At the primordia stage, the normal formation of fruiting body was blocked by sealing the plastic dish with parafilm. The periods requiring for the formation of primordia and fruiting bodies were examined on the dish culture. About 96% and 76% of cultured samples formed primordia and fruiting bodies under the optimal conditions during ten weeks of culture, respectively. These culturing periods, however, were changed by the mechanical injury treatment to mycelia. As other factors affecting the fruiting body formation, the effects of light and cold shock have been tested. No fruiting formation was observed on the cultured dishes under the dark. The cold shock treatment by storing cultured dishes for one day at $4^{\circ}C$ did not have any significant effects in the fruiting body formation. Spores of fruiting bodies acquired from the petri dishes could be germinated on culture media at $28^{\circ}C$. These results suggest that the fruiting bodies of P. ostreatus can be formed on the experimental petri dish and this dish-culturing method is useful for understanding of the developmental process of P. ostreatus in the laboratory. Furthermore, the dish-culturing method is able to shorten the life cycle of P. ostreatus without requiring large area and expensive device.

• Journal of Embryo Transfer
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• v.29 no.3
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• pp.201-206
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• 2014

The objectives of the study were to determine an effective culture dish, culture duration and protein supplementation in medium for in vitro maturation (IVM) of oocytes of native zebu cows in Bangladesh. The ovaries of cows were collected from local slaughterhouse followed by aspiration of follicular fluid. The cumulus-oocyte-complexes (COCs) with more than 3 compact cumulus cell layers were cultured in tissue culture medium (TCM) 199 for maturation. The maturation of oocytes was determined by observing polar body under microscope. To determine an effective culture dish, 130 COCs derived from 48 ovaries in a well of 4-well dish and 102 COCs derived from 36 ovaries in drops covered with mineral oil within 35 mm petri dish were cultured for 24 hours. The rate of maturation of oocytes did not vary between 4-well dish ($51.3{\pm}15.0%$) and drops in petri dish ($52.4{\pm}11.6%$). To determine the effective culture duration, 185 COCs derived from 62 ovaries were cultured in drops for 18, 21, 24 and 27 hours. The rate of maturation of occytes ranged from $51.9{\pm}9.4%$ (18 hours) to $59.0{\pm}17.1%$ (27 hours) and the difference in maturation rate among different culture durations was not significant (P>0.05). To determine an effective protein supplementation, 63 oocytes from 19 ovaries were cultured separately in TCM 199 supplemented with either fetal bovine serum (FBS) or bovine serum albumin (BSA). The rate of maturation was significantly (P<0.01) higher in medium supplemented with FBS ($55.63{\pm}16.19%$) than that of BSA ($14.82{\pm}9.36%$). In conclusion, COCs of native zebu cows can be cultured for IVM either in 4-well culture dish or droplets in petri dish for 18 to 27 hours in medium supplemented with FBS.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.4
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• pp.345-350
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• 2003

A series of aqueous extracts and residues from leaves, stems, roots, pods and seeds of Korean black soybean (Glycine max (L.) Merr.) were assayed against alfalfa (Medicago sativa) and barnyard grass (Echinochloa crus-galli) to determine their allelopathic activities through petri-dish and greenhouse experiments, and the results showed highest inhibition in the extracts or residues from the seeds, and followed by pods. The extracts of 40g dry tissue $\textrm{L}^{-1}$ applied on filter paper in petri-dish bioassay significantly inhibited root growth of alfalfa, and especially extracts from seeds and pods reduced root length of alfalfa more than those from leaves, stems, or roots. Plant height, root length, shoot and root dry weights of barnyard grass were reduced significantly by residue incorporation of seeds and pods as the incorporated amount increased. These results suggest that black soybean plants had herbicidal potential, and their activities were exhibited differently depending on plant parts.