• 대한소아치과학회지
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• 제40권4호
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• pp.321-327
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• 2013

법랑모세포섬유종은 드물게 나타나는 진성 혼합 치성 종양으로 10~20대의 어린 환자의 하악에서 호발하는 경향을 보인다. 법랑모세포섬유종의 치료는 종양부위의 제거를 원칙으로 하고 있다. 종양부의 제거는 보존적 방법과 광범위한 제거를 선택할 수 있으나 이는 아직 논쟁이 되는 사항이며 저자에 따라 주장하는 바가 다르다. 이는 법랑모세포섬유종의 재발률과 악성으로의 전이 가능성 때문이다. 본 증례는 상악 우측 유구치의 미맹출을 주소로 내원한 4세 환아에서 발생한 법랑모세포섬유종에 대하여 묘사하고 있다. 법랑모세포섬유종을 적출술과 소파술을 이용하여 성공적으로 치료 하였기에 이에 대한 문헌고찰 및 증례를 보고하고자 한다.

• 대한치과교정학회지
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• 제20권3호
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• pp.447-462
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• 1990

The objective of this experiment is to observe structural differences in the othodontic movement of vital (control group) and devitalized (experimental group) teeth in the mongrel dogs. The 5 utilized dogs in this experiment were approximately 1 year of age and their average weight was about 12 Kg. Endodontic therapy was performed on the 2nd premolars in upper & lower jaws of each animal under the general anesthesia by intravascular injection of 25mg/kg of pentobarbital sodium. The canals of the teeth were obturated by using gutta percha in conjunction with root canal sealer (AH26 Densply). One of the roots in the 2nd premolars was hemisected to make an extraction space for the devitalized teeth to be moved. The edgewise technique was employed for the movement of the teeth. Orthodontic models and intraoral roentgenograms were taken before and after orthodontic tooth movement. The open coil springs (.010 x .040) were used at interbraket space in order to provide equal forces (75gm) between the teeth in each arch wire. After 13 weeks of active orthodontic tooth movement, dogs were sacrified and the experimental results w ere examined through the intraoral radiography, microscopic examination and scanning electromicroscopic examination at the root sulfate. From the results of the study, the following conclusions may be drawn: The root resolution and cemental deposition were observed within the pressure and tension site in both group. 2. The root resorbed lacunae were observed in the cementum and/or into the dentin in both group. 3. The prominent osteoblastic activities were observed on the alveolar margin in the tension site in both group. 4. A few of blood vessels were observed in the pressure site, but also lots of blood vessels were observed in the tension site especially in the periphery of the alveolar bone in both group. 5. In the pressure site, resorbed lacunae were formed with deep and narrow cavity in the control group; the shallow and wide cavity in the experimental group. 6. In the pressure site, the repaired cementum or cementoid tissue was lined on cementum in the experimental group, but not in the control group. 7. There was no significant difference between external root resolution of endodontically and vital teeth when both were subjected to orthodontic forces.

• Journal of Periodontal and Implant Science
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• 제31권3호
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• pp.555-564
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• 2001

The purpose of this study is to evaluate the bioresorbability of Calcium Polyphosphate added with $Na_2O$ and chitosan. Though calcium phosphate ceramics meet some of the needs for bone replacement, they have some limitation of unresorbability and fibrous encapsulation without direct bone apposition during bone remodelling. To solve these problem, we developed a new ceramic, calcium polyphosphate(CPP), and report the biologic response to CPP in extraction sites of beagle dog. Porous CPP granules were prepared by condensation of anhydrous $Ca(H_2PO_4)_2$ to form non-crystalline $Ca(PO_3)_2$. CPP granules added with $Na_2O$ and chitosan were implanted in extraction sockets and histologic observation were performed at 12 weeks later. Histologic observation at 12 weeks revealed that CPP matrix were mingled with and directly apposed to new bone without any intervention of fibrous connective tissue. CPP granules added with chitosan were well adatped without any adverse tissue reaction and resorbed slowly and spontaneously. CPP granules added with $Na_2O$ and chitosan show multinucleated giant cells and osteoblast-like cells around grafted material and newly formed bone. This result revealed that CPP, regardless of its additive component, had a high affinity for bone and had been resorbed slowly. From this results, it was suggested that CPP is promising ceramic as a bone substitute and addition of $Na_2O$ and chitosan help biodegradation. In further study , it will be determined which concentration of $Na_2O$ help biodegradation and the other additive components increase the degradation rate.

• Journal of Periodontal and Implant Science
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• 제35권4호
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• pp.939-948
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• 2005

The role of the periosteum on osteointegration of $Bio-Oss^{(R)}$(Geistlich, Wolhusen/Switzerland) was studied in rabbit calvarial defect. 12 New Zealand white male rabbits between 2.8 and 4 kg were included in this randomized, blinded, prospective study. Each rabbit was anesthetized with Ketamine HCl(5 mg/kg) and Xylazine HCl(1.5 ml/kg). An incision was made to the bony cranium and the periosteum was reflected. Using a 6-mm trephine bur(3i. USA), four 8-mm defects were created with copious irrigation. The defects were classified into barrier membrane($Tefgen^{(R)}$, Lifecore Biomedical. Inc, U.S.A.) only group as a control, $Bio-Oss^{(R)}$ with barrier membrane group, $Bio-Oss^{(R)}$ with periosteum covering group, and $Bio-Oss^{(R)}$ without periosteum covering group. There were 2 rabbits in each group. The wound was closed with resorbable suture materials. Rabbits were sacrificed using phentobarbital(100 mg/kg) intravenously at 1, 2, and 4 weeks after surgery. The samples were fixed in 4% paraformaldehyde, and decalcified in hydrochloric acid decalcifying solution(Fisher Scientific, Tustin, CA) at $4^{\circ}C$ for 2-4 weeks. It was embedded in paraffin and cut into 6 ${\mu}m$ thickness. The sections were stained with H & E and observed by optical microscope. The results were as follows; 1. The periosteum played an important role in osteointegration of $Bio-Oss^{(R)}$ in bone defects. 2. When the periosteum remained intact and $Bio-Oss^{(R)}$ was placed on the defect, $Bio-Oss^{(R)}$ with periosteum covering has been incorporated into the newly formed bone from 2-week postoperatively. 3. When the periosteum was removed at the surgical procedure, invasion of connective tissue took place among the granules, and new bone formation was delayed compared to periosteum covering group. Therefore, when the bone grafting was performed with periosteal incision procedure to achieve tension-free suture, the integrity of the overlying periosteum should be maintained to avoid fibrous tissue ingrowth.

• 대한소아치과학회지
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• 제33권1호
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• pp.35-42
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• 2006

정상적인 치열의 형성 및 치아의 생성과정을 분석하고 실제로 임상에 적용하기 위해 치아의 발생과정을 연구하는 것은 매우 중요하다. 본 연구는 이식된 태아 치아싹의 석회화가 가능하고 정상적인 구조로 발육할 수 있는지 밝히기 위해, 그리고 악골 자체가 새로운 이식 공여부로 사용가능한지 알기 위해 흰쥐의 발치와에 치아싹을 이식하여 치아의 형성을 살펴보고자 하였다. 이를 위하여 성숙한 흰쥐의 상악 제 1구치를 발거한 발치와에 13.5일된 태아쥐의 치아싹을 이식한 후 4, 8주 후에 희생하여 조직학적 및 방사선학적으로 관찰하여 다음과 같은 결과를 얻었다. 1. 이식된 치아싹에서 상아질과 백악질, 치수, 치주인대의 소견을 갖는 석회화된 조직이 형성되었다. 2. 형성된 경조직 및 연조직은 정상적인 치아형성과정에 비해 발육이 늦었으며 유착 및 골양상아질(osteodentin)과 같은 비정상적 조직이 관찰되었다. 3. 석회화된 조직은 8주내에 악궁으로 맹출하는 소견이 관찰되지 않았다.

• Journal of Periodontal and Implant Science
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• 제51권3호
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• pp.213-223
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• 2021

Purpose: The atmospheric pressure plasma jet (APPJ) has been introduced as an effective disinfection method for titanium surfaces due to their massive radical generation at low temperatures. Helium (He) has been widely applied as a discharge gas in APPJ due to its bactericidal effects and was proven to be effective in our previous study. This study aimed to evaluate the safety and effects of He-APPJ application at both the cell and tissue levels. Methods: Cellular-level responses were examined using human gingival fibroblasts and osteoblasts (MC3T3-E1 cells). He-APPJ was administered to the cells in the experimental group, while the control group received only He-gas treatment. Immediate cell responses and recovery after He-APPJ treatment were examined in both cell groups. The effect of He-APPJ on osteogenic differentiation was evaluated via an alkaline phosphatase activity assay. In vivo, He-APPJ treatment was administered to rat calvarial bone and the adjacent periosteum, and samples were harvested for histological examination. Results: He-APPJ treatment for 5 minutes induced irreversible effects in both human gingival fibroblasts and osteoblasts in vitro. Immediate cell detachment of human gingival fibroblasts and osteoblasts was shown regardless of treatment time. However, the detached areas in the groups treated for 1 or 3 minutes were completely repopulated within 7 days. Alkaline phosphatase activity was not influenced by 1 or 3 minutes of plasma treatment, but was significantly lower in the 5 minute-treated group (P=0.002). In vivo, He-APPJ treatment was administered to rat calvaria and periosteum for 1 or 3 minutes. No pathogenic changes occurred at 7 days after He-APPJ treatment in the He-APPJ-treated group compared to the control group (He gas only). Conclusions: Direct He-APPJ treatment for up to 3 minutes showed no harmful effects at either the cell or tissue level.

• Journal of Periodontal and Implant Science
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• 제52권3호
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• pp.206-219
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• 2022

Purpose: This study was performed to evaluate the influence of local application of thymoquinone (TQ) on bone healing in experimental bone defects infected with Porphyromonas gingivalis (PG). Methods: Forty-two female rats were randomly divided into 6 groups. A bone defect was created on the right tibia of all animals. The PG, PG/collagen membrane (COL) and PG/TQ/COL groups were infected with PG. In the COL and PG/COL groups, the defects were covered with a COL; in the TQ/COL and PG/TQ/COL groups, the defects were covered with a TQ-containing COL. After 28 days, all animals were sacrificed. Quantitative measurements of new bone formation and osteoblast lining, as well as semiquantitative measurements of capillary density and tissue response, were analyzed. Furthermore, the presence of bacterial infections in defect areas was evaluated. Results: The new bone formation, osteoblast number, and capillary density were significantly higher in the TQ groups than in the control groups (P<0.001, P<0.001, and P<0.01, respectively). In a comparison between the TQ/COL group, with a TQ-containing COL (TQ/COL), and the PG-infected TQ-containing COL (PG/TQ/COL) group, the newly formed bone and capillary density were higher in the TQ/COL group (P<0.01). When the control group was compared to the PG, PG/COL, and PG/TQ/COL groups in terms of tissue response, the differences were statistically significant (P<0.001, P=0.02, and P=0.041, respectively). The intensity of the inflammatory cell reaction was higher in the PG, PG/COL, and PG/TQ/COL groups (P<0.05). Conclusions: Within the limitations of this study, the local application of a TQ-containing COL positively affected bone healing even if the bone defects were infected. The results suggest that TQ increased angiogenesis and showed promise for accelerating bone defect healing. Further research is warranted to support these findings and reach more definitive conclusions.

• 대한치과교정학회지
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• 제25권4호
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• pp.433-445
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• 1995

교정력에 의한 성견의 치아이동시 발생하는 조직변화 중 염증과 면역반응에 중요한 역할을 하는 대식세포와 T-림파구의 치주조직내 활성도를 교정력의 크기와 기간에 따라 알아보고자 하였다. 생후 1년 6개월된 성견 6마리 중 5마리의 실험군과 1마리의 대조군이 연구되었는데 실험군에서 하악 좌측에는 강한 힘(250-300g)을, 하악 우측에는 약한 힘(50-75g)을 제1소구치와 제2소구치 사이에 open-coil spring으로 적용하였으며, 실험군의 성견을 각각 12시간, 24시간, 3일, 1주, 2주에 희생시켜 얻은 조직에 $\alpha$-1-antichymotiypsin과 CD3를 이용한 면역조직화학적 염색을 시행하였다. 결과적으로 염증세포침윤은 약한 힘을 준 경우보다 강한 힘을 준 경우에서 3일째까지 더 많이 나타나다가 7일이후에는 차이가 없어졌고 견인측보다는 압박측에서 많이 나타났으며, 압박측의 파골세포 활성은 12시간째에서부터 관찰되어 증가하다가 7일째부터 감소되었는데 약한 힘을 준 경우에서보다 강한 힘을 준 경우에서 더 많이 나타났다. 견인측 치주인대의 단절과 혈관확장은 12시간째 관찰되어 증가하다가 3일째 이후에는 감소되었는데 약한 힘을 준 경우보다는 강한 힘을 준 경우에서 더 많았으나 14일째에는 차이가 없었다. 대조군의 $\alpha$-1-antichymotiypsin발현은 열구상피에서 주로 발현되었고 치주인대, 치수, 골세포에서는 음성반응을 보였으며, 실험군의 $\alpha$-1-antichymotiypsin발현은 견인측보다 압박측에서 많았으며, 3일째까지는 치경부쪽이 약간 많았고, 7일째 이후에는 주로 치경부보다는 치근단쪽에서 더 많이 발현되었다. 약한 힘을 준 경우는 $\alpha$-1-antichymotiypsin 염색이 12시간째부터 나타나서 7일째에 가장 많이 나타나다가 이후 감소되었고, 강한 힘을 준 경우에서는 3일째에서 최고에 달하다가 이후에는 감소되었다. 약한 힘을 준 경우나 강한 힘을 준 경우 모두에서 CD3에 대한 발색은 전치주조직을 통해 거의 음성으로 나타났다.

• Journal of Oral Medicine and Pain
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• 제33권1호
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• pp.15-26
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• 2008

다양한 전신질환으로 입원한 환자들 중에서 구강질환을 진료받기 위해서 내원한 환자의 전신질환 상태와 치과적인 주소 및 진단명 그리고 치료내용을 조사하고 이와 관련된 구강내과적인 주소가 무엇인지를 관찰함으로써 입원한 전신질환자와 구강질환과의 상관관계를 파악하고자 본 연구를 시행하여 다음과 같은 결론을 얻었다. 1. 구강질환을 진료받기 위해서 내원한 환자의 전신질환은 인슐린-비의존성 당뇨병 26%, 뇌경색 25.2%, 뇌내출혈, 다발성 관절증, 고관절증 각각 4.7%, 신경근 및 신경총 장애, 고혈압성 심장 및 신장 질환, 강직성 척추염 각각 2.4% 등의 순서로 나타났다. 2. 내원한 환자의 주소는 치통 28.6%, 정기검사 23%, 치아동요 8.7%, 치아지각과민 7.1%, 치주출혈 6.4% 등의 순서로 나타났다. 3. 내원한 환자의 진단된 구강질환은 치은염 및 치주질환 28.9%, 치아우식증 17.1%, 치수 및 치근단주위의 질환 15.1%, 타액선의 질환 10.5%, 치아경조직의 기타질환 8.6% 등의 순서로 나타났다. 4. 내원한 환자의 치료내용은 치주치료 17.95%, 치료거부 16.67%, 구강건조 및 구취 제거를 위한 약물치료 13.46%, 발치치료 12.18%, 보철치료 8.97% 등의 순서로 나타났다. 5. 내원한 환자의 구강내과적인 주소는 16% 이었으며, 구강연조직질환 6.4%, 두개하악장애 5.6% 그리고 구취 4% 등의 순서로 나타났다. 이상의 결과로 입원한 전신질환자를 통해서 전신질환이 있는 환자의 구강질환과 구강질환이 있는 환자의 전신질환에 대해서는 일정부분 파악이 되었으나 향후 구강질환과 전신질환과의 상관관계에 대한 연구가 계속적으로 이루어져야 한다고 사료된다.

• Restorative Dentistry and Endodontics
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• 제31권3호
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• pp.192-202
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• 2006

본 연구의 목적은 흰쥐 상악 대구치를 발거 한 후 급속 냉동보존을 통해 치아를 보관하였을 때 in vivo MTT 검색법을 이용하여 치주인대세포의 활성도를 측정하고자 하였다. 실험 방법은 74마리 4주령의 암컷 Sprague-Dawley계의 흰쥐를 사용하여 각군당 10마리의 쥐에서 상악 좌우 제1, 2 대구치를 발거하여 모두 40개의 치아를 사용하였다. 대조군은 즉시 발치군이며 냉동군은 F medium에 5% Dimethylsulfoxide (DMSO) 6% Hydroxyethyl starch (HES)를 포함한 군(1군), 10% DMSO를 포함한 군(2군) 그리고 $Viaspan^(R)$에 5% DMSO 6% HES(3군), 10% DMSO를 포함한 군 (4군)으로 나누어 1주일간 액체질소에 냉동한 뒤 해동하였다. 냉장군은 F medium (5군)와 $Viaspan^(R)$ (6군)에 넣어 1주간 $4^{\circ}C$ 냉장고에서 보관하였다. 냉동 및 냉장보존한 후에는 in vivo MTT검색법을 시행하였다. 개개 치아의 치근면 단위면적으로 표준화하기 위해 치근을 1% eosin Y 용액에 12시간 담근 후 1% acid alcohol로 용해시켜 530 nm에서 측정한 흡광도 값을 in vivo MTT 측정값으로 나누었다. 통계 분석을 위해 Two way ANOVA와 Duncan's Multiple Range Test를 95% 신뢰 구간에서 시행하였다. 그리고 각 군당 2개의 치아를 같은 방법으로 처리한 후 $10{\mu}m$ 두께로 냉동 절단하여 광학 현미경과 편광 현미경하에서 관찰하였다. 1, 2군은 3, 4군보다. 높은 흡광도를 나타내었으며 6군은 5군보다. 높은 흡광도를 나타내었다(p<0.05). 광학 현미경 관찰에서 MTT 결정은 파란색을, 편광현미경에서는 밝은 오렌지색을 나타내었고 전체적으로 in vivo MTT검색법의 결과와 같은 양상을 나타내었다. 본 연구의 결과 5%, 10% DMSO를 사용한 F medium 배지로 냉동보존한 경우가 $Viaspan^(R)$을 배지로 냉동하였을 때나 냉장보존 하였을 때보다 통계적으로 유의차 있게 높은 세포 활성도를 나타내었다(p<0.05).