The responses of five varieties and three cultivars of pea (Pisum sativum) to Rhizobium inoculation on nodulation, growth, nitrogenase activity, dry matter production and N uptake were investigated. The pea varieties were IPSA Motorshuti-l, IPSA Motorshuti-2, IPSA Motorshuti-3, BARI Motorshuti-l, BARI Motorshuti-2 and the cultivars were 063, Local small and Local white. Fifty percent seeds of each pea variety/cultivar were inoculated with a mixture of Rhizobium inoculants at rate of 15g/kg seed and the remaining fifty percent seeds were kept uninoculated. The plants inoculated with Rhizobium inoculant significantly increased nodulation, growth, nitrogenase activity, dry matter production and N uptake. Among the varieties/cultivars, BARI Motorshuti-l performed best in almost all parameters including nitrogenase activity of root nodule bacteria of the crop. There were positive correlations among the number and dry weight of nodules (r=$0.987^{**}$, $0.909^{**}$), nitrogenase activity of root nodule bacteria (r=$0.944^{**}$, $0.882^{**}$), dry weight of shoot (r=$0.787^{**}$, $0.952^{**}$), N content (r=$0.594^{**}$, $0.605^{**}$) and N uptake (r=$0.784^{**}$, $0.922^{**}$) by shoot both at flowering and pod filling stages of the crop, respectively. It was concluded that BARI Motorshuti-l in symbiotic association with Rhizobium inoculant performed best in recording nitrogenase activity, dry matter production and N uptake by pea.
Jun, Ha Joon;Jeon, Eui Hwan;Kang, Soo In;Bae, Geun Hye
Horticultural Science & Technology
/
v.32
no.6
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pp.812-818
/
2014
Raising seedlings is important for fruit crops and is especially significant for strawberries as it accounts for 80% of their cultivation. However, there are few studies on raising seedlings of strawberries by hydroponics. Since strawberries show clear differences in growth characteristics based on cultivar, it is necessary to develop suitable fertilizer formula, concentration and pH for each cultivar, and also to examine the amount of nutrient feeding appropriate for each medium type. A key to raising seedlings of strawberries by hydroponics is the development of strategies to manage the concentration of nutrient solution. The mother plants of 'Daewang' strawberries were planted on hydroponics benches filled with cocopeat on March 28, 2012. Three nutrient solution treatments were employed during the term of raising seedlings: a type that supplied EC $0.6dS{\cdot}m^{-1}$ nutrient solution for 30 days and only water for 20 days [0.6 (30) + 20]; a type that supplied EC $1.2dS{\cdot}m^{-1}$ nutrient solution for 30 days and only water for 20 days [1.2 (30) + 20]; and a type that supplied EC $1.2dS{\cdot}m^{-1}$ nutrient solution for 50 days [1.2 (50)]. The plants were then planted on hydroponics benches filled with cocopeat on September 20, and managed with EC $0.6-0.8dS{\cdot}m^{-1}$ strawberry nutrient solution developed by Yamazaki. After planting, shoot growth, flowering rate and fruit quality of the first cluster were investigated. The petiole length, leaf length, leaf width and crown diameter showed the highest grown in the [1.2 (50)] treatment, followed by [1.2 (30) + 20], and then [0.6 (30) + 20], indicating that the higher concentration of nutrient solution was preferable for raising seedlings. However, the growth differences among treatments gradually disappeared as growth continued, and the crown diameter especially grew to exhibit almost no difference at all among treatments. The point of flowering came first in [0.6 (30) + 20], followed by [1.2 (30) + 20] and then [1.2 (50)]. The [0.6 (30) + 20] treatment showed much earlier flowering than other treatments, which implies that low-concentration nutrient solution may be beneficial to the flowering of 'Daewang' strawberries while raising seedlings. There was no statistically significant difference among treatments in fruit length, fruit diameter and fruit firmness. Fruit weight in [1.2 (50)] treatment was significantly higher than other treatments. However, soluble solids of fruit was the lowest in [1.2 (50)] treatment. Together, the results of this experiment imply that it is better to supply EC $0.6dS{\cdot}m^{-1}$ solution for 30 days and then supply only water for 20 days to adequately manage concentration of nutrient solutions during the period of raising seedlings of strawberries by hydroponics.
The rapid advance of technology has accelerated global warming. As 50.4 percent of South Korea's population is concentrated in the Seoul Metropolitan Area, which has become a considerable emitter of greenhouse gases, the city's average temperature is expected to increase more rapidly than in other areas in the country. A rise in the average temperature would affect everyday life and urban ecology; thus, appropriate measures to cope with the forthcoming disaster are in need. This study analyzed the changes in plant phenological phases from the past to the present based on temperatures (average temperature of Feb, Mar, April) observed in seven different weather stations nearthe Seoul Metropolitan Area (Ganghwa, Seoul, Suwon, Yangpyeong, Icheon, Incheon, and Paju) and the first flowering dates of Plum tree (Prunus mume), Korean forsythia (Forsythia koreana), Korean rosebay (Rhododendron mucronulatum), Cherry tree (Prunus serrulate), Peach tree (Prunus persica), and Pear tree (Pyrus serotina). Then, RCP (Representative Concentration Pathways) 2.6 and 8.5 scenarios were used to predict the future temperature in the Seoul Metropolitan Area and how it will affect plant phenological phases. Furthermore, the study examined the differences in the flowering dates depending on various strategies to mitigate greenhouse gases. The result showed that the rate of plant phenological change had been accelerated since the 1900s.If emission levels remain unchanged, plants will flower from 18 to 29 earlier than they do now in the Seoul Metropolitan Area, which would be faster than in other areas in the country. This is because the FFD (First Flowering Date), is highly related to temperature changes. The Seoul Metropolitan Area, which has been urbanized more rapidly than any other areas, is predicted to become a temperature warming, forcing the FFDs of the area to occur faster than in the rest of the country. Changes in phenology can lead to ecosystem disruption by causing mismatches in species interacting with each otherin an ecosystem. Therefore, it is necessary to establish strategies against temperature warming and FFD change due to urbanization.
The main aim of this study is to evaluate the efficiency of two biologically active compounds(Strom and F-760) in control of wheat root rot disease and its causal organisms. Fusarium graminearum, F. oxysporum, F. solani and Bipolaris sorokiniana were used as target organisms. In vitro, the two compounds showed fungicidal effect on all investigated pathogens resulted in suppression of radial growth and mycelial dry weight of them. Under greenhouse conditions, treatment of wheat grains with either Strom or F-760 before cultivation significantly reduced the percent of disease distribution as well as the mean disease rating of plants in both seedling and flowering stages. Fresh and dry weights of plants as well as water maintenance capacity were increased as the result of applying these compounds as seed dressing. Also data showed that the membrane stability of plants was injured as a result of infection with all investigated organisms, while this injury was alleviated when F-760 and Strom were applied. The $K^+$ efflux and the leakage of UV absorbing metabolites was stimulated with fungal infection. However, F-760 and Storm treatment partially retarded the stimulatory effect on leakage of $K^+$ and UV-absorbing metabolites of fungal infected plants. On the other side, the fungal infection had inhibitory effects on pigment fractions(chlorophyll a, b, and carotenoids) biosynthesis in wheat leaves. This retarding effect was partially or completely alleviated as the grains were treated with the applied compounds.
Strong wind of 6.5m/s from electric fan was applied to rice at heading stage to find out the nature of wind damage to panicle. Damaged panicles caused by typhoon were sampled out of field to measure the yield and milling components. Wind-treated glumes were developed into two forms, fertile and sterile, and each of them showed all degree of glume discoloration. The number of fertile grains were higher than sterile grains in a damaged panicle. Averagesdegree of glume discoloration was higher in not flowered glume than in flowered glume. The more recently flowered glumes resulted the higher degree of discoloration among flowered glume. The percent of sterile grain to total number of grains was the highest at stage of just flowering. The glume discoloration representing wind damage at heading state advanced in degree in ten days after treatment compared to that just after treatment. Grain length and grain width were decreased with the increased degree of glume discoloration, while grain thickness was not changed greatly. Chalkiness was a little higher in damaged grain than in normal grain without definite trends by degree of discoloration. Ripening ratio, 1000 grain weight and yield decreased with increased degree of glume discoloration in damaged panicle by typhoon. Decreasing order in yield was white rice, rough rice and brown rice. Ratio of brown rice to rough rice increased, white rice to brown rice decreased, and milling recovery was not changed greatly with increased degree of 히ume discoloration.
This experiment was carrid out to investigate the effect of organic fertilizer and plant growth regulators application on the growth and quality of tomato. The results are summarized as follows. 1. Plant height of tomato was recorded highest when chemical fertilizer plus organic fertilizer was applied, and did not have significant effects in number of leaf. But stem diameter was positively effected by chemical plus organic fertilizer application than chemical fertilizer alone. 2. Flower formation, flower weight, anther weight and ovary weight were generally increased by organic fertilizer application. 3. Fruit-set and number of flower were significantly increased by organic fertilizer application. 4. Deformity fruit was the lowest rate at chemical plus organic fertilizer application when it was 14.7 percent, and it was increased by chemical fertilizer application. 5. Days of ripening was slightly delayed by organic fertilizer application and also flowering date shortened by chemical fertilizer application. 6. Plant growth regulators had positive effects on number of flower, flower weight, anther weight, and ovary weight, and variations of their effect by cluster were apparent. 7. Fruit-set was increased by 2,4-D 10ppm and BA 20ppm treatments but was decreased by treatments of Ethephon 10ppm and control. 8. By the BA 20ppm and 2,4-D 10ppm treatments, the rate of deformity fruit was decreased and fruit ripening date was also shortened.
Arabidopsis trp1 mutant plants, deficient in phosphoribosyI anthranilate transferase (PAT) activity, accumulate anthranilate compounds, which render them blue fluorescence. The visible phenotype of trp1 makes the PAT gene an excellent reporter gene in the mutant. In order to develop a system for the homologous recombination using the phenotypic characteristic of trp1-100, we established optimum conditions for the isolation and regenera tion of protoplast from auxin-conditioned, trp1-100 root cultures. Trvptophan had to be supplemented in the germination medium for the efficient cell division and subsequent plant regeneration. When 10 uM tryptophan was added to the germination medium, we obtained the highest yield of protoplasts ($3{\times}10^6 cells/g$) and the best viability (92%). Thirty percent of root protoplast derived from meristematic cells underwent cell division within 5 days in callus-induction medium. Regenerated rosette leaves (2-3 mm) were transferred to rooting medium and finally acclimated to the soil for flowering.
The effect of sodium sulphate on shoot induction and multiple shoot formation from nodal explants of Vitex negundo L. was tested on Murashige and Skoog's (MS) medium fortified with different auxins, cytokinins and sucrose. Highest percentage $(97.78\%)$ of explants for shoot induction and multiple shoot (20.68/explant) production were observed in the combination treatment of $N^6-Benzyl$ adenine (BA) $(17.80\;{\mu}M/L)$, ${\alpha}-Naphthalene$ acetic acid (NAA) $(2.15\;{\mu}M/L)$ and $5\%$ sucrose supplemented with 100 mg/L sodium sulphate. In vitro raised shoots were rooted on the half-strength MS medium fortified with different concentrations of NAA, Indole-3-acetic acid (IAA), and Indole-3-butyric acid (IBA) alone and in combinations. Among the treatments, $4.90\;{\mu}M/L$ of IBA was found most effective $(95.56\%)$ in inducing roots. The rooted plantlets were shifted to glasshouse for acclimatization and later transferred to the field with cent percent survival. Furthermore, in vitro flowering was observed in the shoots cultured on MS medium supplemented with BA $(8.90\;{mu}M/L)$ and NAA $(1.61\;{\mu}M/L)$.
The purpose of this study is to suggest how to manage healing forests. Field investigation and surveys were conducted to produce results and 313 questionnaires collected from workers in the forestry sector and ordinary people were analyzed. The results were as follows: it is required to preserve flowering plants, scenic trees, and ecological trees in the understory vegetation, and to remove trees that block the forest landscape, leaving about 50 to 60 percent of the understory vegetation. The preferred density order of broadleaf trees was analyzed as follows: Betula platyphylla > Liriodendron tulipifera > Quercus acutissima. The preferred density order of coniferous trees was analyzed as follows: Abies holophylla > Cryptomerias japonica and Chameacyparis obtusa > Larix kaempferi > Pinus densiflora > Pinus koraiensis. The preferred density in healing forests was 81-89% compared to the number of residual trees for quantitative thinning. Specifically, the preferred density were 87% for P. koraiensis, 86% for L. kaempferi, 81% for P. densiflora, 83% for C. japonica, 84% for C. obtusa and 89% for Q. acutissima. In the case of healing forests, it is recommended to periodically conduct a small-scale thinning with different densities according to the species and diameter of trees based on the results of this study.
Kwack, Yong-Bum;Kang, Seong-Mo;Kim, Hong Lim;Kim, Mok-Jong;Kim, Seong-Cheol;Lee, Yong-Bok
Korean Journal of Environmental Agriculture
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v.34
no.4
/
pp.288-293
/
2015
BACKGROUND: This study was conducted to investigate the effect of the unexpected early loss of leaves on a newly-bred kiwifruit on the regrowth of axillary buds the current season and the early growth and development the following year.METHODS AND RESULTS: The vines were defoliated on Jul. 18, Aug. 16, and Sep. 17 in 2012 and on Jul. 16, Aug. 13, and Sep. 12 in 2013. The vines were defoliated 0 (control), 50, and 100% of the total number of leaves on a vine. The regrowth of axillary buds at 30 days after defoliation increased in proportion to defoliation degrees regardless of the defoliated time. Defoliation the previous season did not influence percent budbreak the next season. Percentage of floral shoots of the control vines was 27.4%, each bearing 2-3 flowers. In those vines defoliated 100% in August and September, however, percent floral shoots and number of flowers significantly reduced.CONCLUSION(S): Defoliation in July, August, and September didnot affect percent budbreak the following year regardless of degrees of defoliation. A 100% defoliation in August and September significantly reduced flowering the following year compared to the control; that in August resulted in no floral buds at all.
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