• 농약과학회지
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• 제5권2호
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• pp.37-44
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• 2001

본 연구는 감귤을 부패시키는 Penicillium spp.에 대한 kresoxim-methyl 과 iminoctadine tris(albesilate)의 방제효과를 검정하기 위하여 실시하였다. P. italicum과 P. digitatum의 균사생장에 대한 iminoctadine tris(albesilate)의 $EC_{50}$값은 각각 0.01-0.02와 $0.01{\mu}g$ a.i./mL이었지만, 아직 미동정인 새로운 유형의 Penicillium spp.균은 iminoctadine tris(albesilate)가 $0.64{\mu}g$ a.i./mL들어 있는 PDA배지에서도 거의 균사생장이 억제되지 않았다. 또한 P. italicum과 P. digitatum의 발아와 발아관의 생장에 대한 iminoctadine tris(albesilate)의 $EC_{50}$값은 $0.0013-0.0025{\mu}g$ a.i./mL이었지만 새로운 유형치 Penicillium spp.는 $0.2{\mu}g$ a.i./mL 농도에서도 포자 발아가 거의 억제되지 않았다. P. italicum과 P. digitatum 그리고 미동정 Penicillium spp.의 균사생장에 대한 kresoxim-methyl의 $EC_{50}$은 각각 0.08-0.16, 0.04 그리고 $0.16{\mu}g$ a.i./mL이었으며 P. italicum과 새로운 유형의 Penicillium spp.의 포자 발아 및 발아관 생장에 대한 $EC_{50}$이 $0.04{\sim}0.08{\mu}g$ a.i./mL이며 P. digitatum에 대해서는 $0.01{\sim}0.02{\mu}g$ a.i./mL였다. Kresoxim-methyl과 iminoctadine tris(albesilate)의 저장병에 대한 방제효과를 알아보기 위하여 이들 약제를 각각 $155{\mu}g$ a.i./mL과 $200{\mu}g$ a.i./mL의 농노로 수상살포하고 7일 후 수확하여 90일간 저장한 결과 무처리구와 thiophanate-methyl $700{\mu}g$ a.i./mL을 처리한 대조구에서의 부패율은 각각 $20.3{\pm}10.0%\;and\;19.5{\pm}9.6%$으로 높은데 반하여 tresoxim-methyl과 imonoctadine tris(albesilate) 처리구에서의 부패율은 각각 $3.6{\pm}1.8%$ 과 $5.9{\pm}1.8%$로써 방제효과가 높았다. 이들 처리로부터 저장 후 0, 30, 60, 90일에 각각 iminoctadine tris(albesilate)와 kresoxim-methyl의 잔류량을 조사해본 결과 각 조사 기간 중 iminoctadine tris(albesilate)의 최대잔류량은 0.10 ppm이었고 kresoxlm-methyl의 최대 잔류량은 0.45 ppm이었다.

• Journal of Microbiology and Biotechnology
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• 제30권6호
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• pp.878-884
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• 2020

Penicillium digitatum and P. italicum are the two important postharvest pathogens in citrus, causing about 90% of the total loss of citrus fruit during storage and transportation. Natural fungicides such as essential oils have been widely used instead of chemical fungicides for preventing and controlling postharvest diseases. In this research, p-anisaldehyde exhibited a strong inhibitory effect on P. digitatum and P. italicum, with the minimum inhibitory concentration and minimum fungicidal concentration values of both being 2.00 μl/ml. Additionally, p-anisaldehyde visibly inhibited both the green mold and blue mold development of citrus fruits inoculated with P. digitatum and P. italicum. The mycelia morphologies of these pathogens were greatly altered, and the membrane permeability and cell wall integrity of mycelia were severely disrupted under p-anisaldehyde treatment. These results suggest that the antifungal activity of p-anisaldehyde against P. digitatum and P. italicum can be attributed to the disruption of the cell wall integrity.

• 한국미생물·생명공학회지
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• 제16권3호
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• pp.182-186
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• 1988

원형질체 융합기술에 의한 Penicillium verruculosum 균주개량 가능성을 검토하였다. 영양요 구변이주간의 원형질 융합체 165주중 cellulase 고생산 융합체로 선별된 6주의 cellulase 활성은 야생주에 비해 2배이상, 모균주에 비해 1.2-4.4배의 증가를 나타냈다. Cellulase 고생산 융합체는 유전적 안정성조사, DNA 함량 및 핵염색 비교검토를 통해 핵융합이 일어난 것으로 확인되었고, 핵형이 이수체이며 4 개월 이상의 계대배양 후에도 자연형질 분리가 거의 일어나지 않는 것으로 보아 이들 융합체의 안정성이 매우 높은 것으로 인정되었다.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1606-1608
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• 2014

During an investigation of the fungal diversity of Korean soils, four Penicillium strains could not be assigned to any described species. The strains formed monoverticillate conidiophores with occasionally a divaricate branch. The conidia were smooth or finely rough-walled, globose to broadly ellipsoidal and $2.5-3.5{\times}2.0-3.0{\mu}m$ size. Their taxonomic novelty was determined using partial ${\beta}$-tubulin gene sequences and the ribosomal internal transcribed spacer region. The phylogenetic analysis showed that the isolates belonged to section Lanata-Divaricata and were most closely related to Penicillium raperi. Phenotypically, the strains differed from P. raperi in having longer and thicker stipes and thicker phialides. Strain KACC $47721^T$ from bamboo field soil was designated as the type strain of the new species, and the species was named Penicillium koreense sp. nov., as it was isolated from various regions in Korea.

• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.385-389
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• 2004

The antibody-mix sandwich enzyme-linked immunosorbent assay (Ab-mix sELlS A) system was developed in order to simultaneously detect the extracellular polysaccharide (FPS) of Aspergillus, Penicillium, or Fusarium species using one detection system. The detection limit and detection range of the Ab-mix sELISA towards EPS of Penicilliun citrinum were not changed, and those towards Fusarium moniliforme EPS were changed a little compared to that of individual sandwich ELISA [9, 10]. The fungal culture filtrates of Aspergillus and Penicillium species showed nearly similar reactivity towards Ab-mix sELISA as that of sELISA using the MAb lB8 alone [9]. Also, the fungal culture filtrates of Fusarium species showed nearly the same reactivity towards Ab-mix sELISA as that of sELISA using the MAb lB8 alone [10]. Thus, this ELISA system showed that the three genera of molds, Aspergillus, Penicillium, or Fusarium, which are three major important molds producing mycotoxins in food or agricultural commodities, could be detected at the same time, using one detection system.

• Applied Biological Chemistry
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• 제39권6호
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• pp.455-459
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• 1996

잔디밭 및 초지에서 많이 발생하는 다년생 포복성 잡초인 토끼풀을 효과적으로 방제할 수 있는 새로운 미생물제초제를 개발하기 위하여 토끼풀 및 유사 잡초의 병반으로부터 980여주의 곰팡이 균주를 분리하고 각 균주에 대한 제초활성 검정을 통하여 토끼풀에 대한 제초활성이 우수한 F40362, F40496 및 F40497 3균주를 선발하였다. 선발된 3 균주는 동정한 결과 모두 Penicillium속에 속하는 균주이었다. 각 균주의 포자현탁액을 사용하여 토끼풀에 대한 활성을 검정하였을 때 $1{\times}10^7$ 포자수/ml 농도에서 제초활성이 나타났으며 포자현탁액으로서는 멸균수보다 이들 각 균주의 배양액을 사용하는 것이 효과적이었다. 한편 동일 농도에서 대조식물인 한국 들잔디에 대하여 병해는 없었다.

• 한국균학회지
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• 제23권4호통권75호
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• pp.348-353
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• 1995

Poly (3-hydroxybutyrate)(PHB) 의 Penicillium pinophilum에 의한 생분해성을 변형 Sturm Test 법으로 조사하였다. 활성오니를 사용한 경우보다 PHB의 생분해성이 비교적 재현성있게 측정되었으며 PHB의 생분해를 가장 빠르게 진행시키는 최적 포자 접균량은 1 %(v/v)이었다. 생분해 속도는 시료의 표면적에 따라 증가하였으나 비례적으로 상승하지 않아 분해가 시료의 표면 뿐만 아니라 내부에서도 진행됨을 나타내었다. 반응 배지내의 질소원 함량에 따라 PHB의 생분해 속도는 증가하다가 점근값을 보여 질소원이 depolymerase 효소의 합성에 필요한 원소임을 보였다.

• Mycobiology
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• 제35권3호
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• pp.159-161
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• 2007

Pine tree death caused by pine wood nematode(PWN) involves phoretic relationships between PWN and its vector Japanese pine sawyer beetle(JPS). In an effort to understand the diversity of fungi involved in PWN life cycle, a total of 176 fungal isolates were collected from PWNs, adults and larvae of JPS, PWN-diseased Japanese black pine that was cut down in 2005 at Jinju, Korea. Based on microscopic observation and colony morphology, and sequence analysis of the ITS rDNA, the fungal isolates were identified at the level of genus. Three genera including Mucor, Ophiostoma, and Penicillium were identified from PWN. Two genera of Ophiostoma and Penicillium were discovered from JPS larvae. Frpm JPS adult beetles, nine genera of Aspergillus, Gibberalla, Hypocrea, Irpex, Leptosphaeria, Ophiostoma, Penicillium, and Plectosphaerella and unknown basidio-mycetes were found. Ten genera from PWN-infected weed were confirmed as Bionectria, Botrytis, Camarops, Fusarium, Hypocrea, Nectria, Mucor, Ophiostoma, Penicillium, and Trichoderma. Penicillium and Ophiostoma were commonly distributed on PWN and its vector and host. This is first report of the fungi associated with PWN and its vector and host in Korea.

• Mycobiology
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• 제42권3호
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• pp.274-278
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• 2014

Penicillium georgiense was isolated from sandy beach soil from Batu Ferringhi beach, Penang Island, Malaysia. The identification was based on morphological characteristics and phylogenetic analysis of internal transcribed spacer regions and ${\beta}$-tubulin sequences. To the best of our knowledge, this is the first report of P. georgiense in Malaysia.

• Journal of Microbiology
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• 제39권4호
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• pp.331-333
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• 2001

The production of oilgosaccharides using Penicillium citrium cells at high sugar concentrations was investigated at 50$\^{C}$ and pH 5.0. Both 1-kestose and neokestose were produced form sucrose, while both nystose and tetrasaccharide were produced from 1-kestose. However, no reaction product was obatined from neogructo-oligosaccharides such as neokestos. Based on these experimental rsults, a hypothetical reaction route was proposed to illustrate how neofructor-oilgosaccharids are formed from 1-kestose.