• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1972년도 춘계종합 학술대회 초록집
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• pp.1.2-1
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• 1972

만성중이염의 원인균에 대해서는 여러가지 방면으로 고찰되어 왔다. 저자들은 시대에 따르는 원인균의 분포와 아울러 새로운 항생제제의 개발에 따르는 내성 및 감수성의 정도와 변화 등을 고찰하기 위하여 임의로 선택된 중이염환자의 이루에서 채취된 세균에 대해 이를 분류하고 각종 항생제에 대한 감수성을 검사하였기에 보고한다. 65이에서 분류된 65균주에 대해 Ampicillin Chloramphenicol, Erythromysin, Kanamycin, Gentamycin, Orbenin, penicillin, Streptomycin, Terramyin 및 Colimycin등 10종류의 항생제제에 대한 감수성 청사를 하였다. Gram-negative 군인 pseudomonas(12례, 18.5%)에서는 Colimycin에 91.7% 및 Gentamycin에는 75%의 순으로 감수성이 높았고 proteus (11례 16.9%)에 가장 감수성이 높은 약제는 Ampicillin 81.8%이고 Genta-mycin에 81.8% 등이었다. Gram-positive인 Staphyloccus aureus(19례 29.2%)에서는 Erythromyin에 100% Orbenin에 94.7%의 순으로 감수성이 높았다.

• 한국수정란이식학회지
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• 제28권4호
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• pp.337-342
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• 2013

Salmonellosis is one of the life-threating diseases of goat in Bangladesh. Therefore, the present study was designed to study the prevalence of Salmonellosis, and isolation and characterizations of the Salmonella spp. from apparently healthy and diarrheic goat. A total of 47 faces samples were collected from selected place and cultured onto different prescribed medium to isolate it. In this study, 12.76% (6/47) samples were found to be positive for Salmonella spp. During culture on SS agar medium, all of the Salmonella isolates produced round, smooth, opaque, translucent and black color colonies on SS agar media. All of the isolated Salmonella spp. fermented dextrose, maltose and mannitol with production of acid and gas but did not ferment sucrose and lactose. However, these isolates had showed Indole and Voges-Proskauer test negative, Methyl-Red test positive. All of these isolates were subjected to rapid plate agglutination test with polyvalent "O" (Poly 'O') and polyvalent "H" (poly 'H') antisera where positive agglutination were observed. They were highly sensitive to ciprofloxacin, spiramycin and gentamycin; moderately sensitive to oxytetracyline, streptomycin and amoxicillin; less sensitive to sulphamethoxazole and resistant to penicillin-G. Based on the present findings, it may be concluded that the investigated Salmonella spp. from goats might be S. typhimurium, S. enteritidis, S. brandenburg, S. salford, S. newbrunswick, S. newport or S. dublin. Further study will be needed, therefore it requires further characterization using other serological and molecular techniques.

• 대한수의학회지
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• 제41권4호
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• pp.511-521
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• 2001

Microorganisms were isolated and identified from bovine 296 quarters which showed positive reaction by California Mastitis Test (CMT) in 40 farms of Jeju from September 1999 to June 2000. The organisms associated with the mastitis of bovine were 11 different bacterial species in this study. Which of them, Staphylococcus aureus was the most predominant species as 152 (51.4%) isolates. Other identified species included 49 (16.5%) coliform, 47 (15.8%) Streptococcus dysgalactiae, 15 (5.1%) Bacillus spp., 8 (2.7%) Staphylococcus epidermidis, 6 (2.1%) Streptococcus agalactiae, 5 (1.7%) Enterococcus faecalis, 5 (1.7%) Corynebacterium spp., 3 (1.0%) Streptococcus uberis, 1 (0.3%) Pseudomonas aeruginosa and 1 (0.3%) Pasteurella haemolytica. Almost of all the islolated beacterial species showed high sensitivity against kanamycin (98.6%), cephalothin (98.0%), streptomycin (94.9%), gentamicin (94.6%), ampicillin (92.2%) and polymyxin B (90.2%). On the contrary, they showed resistance against penicillin (47.0%), tetracycline (37.2%), cefazolin (26.0%), bacitracin (22.6%) and erythromycin (19.9%). Eighty-one isolates were not resistant to any antibiotics and 215 drug resistant isolates showed 89 different drug resistance patterns from single to nine multiple antibiotics resistance patterns.

• Journal of Chest Surgery
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• 제12권4호
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• pp.403-410
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• 1979

A Clinical analysis of 64 patients of thoracic empyema was done who received surgical intervention at Dept. of Thoracic Surgery of the Chosun University Hospital in the period of 3 years from September 1976 to October 1979. Following was the results: 1. Seven cases [10.9%] were under the age of 15 years, 16 cases [25%] was between 15-30 years and 41 cases [64.1%] was above the age of 30 years. A proportion of children and adult was 1:8. 2. Male and female ratio was 3:1. Right and left side pleural cavity ratio was 2.4:1. 3. Predisposing factors were pneumonia [35.9%] and pulmonary tuberculosis [28.1%]. 4. Most frequently encountered symptoms were dyspnea, cough, chest pain and fever in order. 5. Etiologic organisms were confirmed in 39 cases [86.7%] which requested in 45 cases. Staphylococcal infections were 11 cases and streptococcal, pneumococcal pseudomonas infection was infected in order. 6. Pneumothorax was associated with empyema on 21 cases [32.8%]; among those 13 cases [61.9%] were tuberculous in nature. 7. Sensitivity test was revealed that Minocin was most very sensitive drug, and next Erythromycin, Gentamycin and Penbrex in order. But most resistant drugs were Penicillin, Kanamycin, Streptomycin and Tetracycline in order. 8. Treatments were combined with antibiotics therapy and several surgical procedures for empyema. 26 cases [40.6%] were treated with closed thoracotomy drainage, 17 cases [26.6%] with open thoracotomy tube drainage and 9 cases decortication and 9 cases thoracoplasty. 9. 2 death cases occurred in 64 cases of thoracic empyema, and 79.7% cases were discharged with recovery and improvement.

• International Journal of Oral Biology
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• 제40권1호
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• pp.41-50
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• 2015

The aim of this study was to identify bacteria isolated from the oral cavities and to determine their antimicrobial susceptibility against eight antibiotics. The bacterial strains were obtained from the Korean Collection for Oral Microbiology (KCOM). The bacteria were identified by comparing 16S rDNA sequences at the species level. The data showed that 77 bacterial strains were predominantly identified as streptococci (49.4%) and staphylococci (14.3%). Minimum inhibitory concentrations (MIC) were determined using a broth dilution assay to test the sensitivity of the bacterial strains. The MIC values of the oral bacterial strains against antibiotics were different. Streptococci were sensitive to clindamycin, cefuroxime axetil, and vancomycin, and they were resistant to tetracycline. Staphylococci also were sensitive to clindamycin, cefuroxime axetil, and vancomycin, and they were resistant to penicillin antibiotics. Gramnegative bacterial strains were sensitive to tetracycline and were resistant to clindamycin. These results suggest that the antimicrobial susceptibility test is necessary in deciding the prescription for antibiotics, to prevent the misuse or abuse of antibiotics.

• 대한수의학회지
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• 제14권1호
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• pp.91-97
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• 1974

A total of 820 dairy cattle were examined formastitis by California mastitis test method and 181 dairy cattle were examined bacteriologically according to the method of Connecticut mastitis control program. The results obtained were as followings: 1. Milk samples from 1.231(38.1%) of 3,225 quarters and 568(69.3%) of 820 dairy cattle were positive for mastitis by California mastitis test method. 2. Among 181 cattle examined bacteriologically by the method of Connecticut mastitis control program 159(22.9%) quarters of 102(56.4%) cattle were infected. Clinical mastitis was found in 5 cattle. 3. Important causative organisms were found to de Staphylococcus aureus(44.8%), Streptococcus agalactiae(21.5%), and non-str, agalactiae Streptococcus(26.4%). Corynebacterium ssp., Pseudomonas spp., Coliforms and Yeasts were also occasionally found to be causative organisms. 4. Strains of Staphylococcus aureus isolated from bovine udders were tested for sensitivity to antibiotics. The rates of resistant strain were 13.7%, 11.8%, 8.8%, 5.9%, 100%, 4.9% and 4.9% for penicillin G, streptomycin, terramycin, erythromycin, chloramphenicol, ampicillin and kanamycin, respectively.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제30권3호
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• pp.211-217
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• 2004

Previously, strains of Streptococci genera were isolated from osteomyelitis caused by the post-infection after extraction. In present study, to test the sensitivity of the Streptococci strains against seven antibiotics, penicillin G, amoxicillin, tetracycline, ciprofloxacin, cefuroxime, erythromycin, clindamycin, and vancomycin, minimum inhibitory concentration (MIC) was performed using broth dilution assay. Our data showed that the value of MIC of the Streptococci against antibiotics were different among the strains. In addition, the degree of resistance to antibiotics of Streptococci strains was mainly depended on the origin of isolation. Our results suggest that the development of the rapid and accurate method to detect the antibiotics-resistant bacteria is need to prevent the misuse or abuse of antibiotics and outbreak of antibiotics-resistant bacteria.

• Journal of Microbiology and Biotechnology
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• 제5권3호
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• pp.138-142
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• 1995

Bacillus thuringiensis strain BT-209 was isolated from a soybean grain dust sample in Korea. The strain BT-209 produced two different sizes of cuboidal crystals and one spore in the cell. In the biochemical characterization, the strain BT-209 showed negative reactions on the production of urease, and the utilization of citrate and sucrose. Examination of its antibiotic resistance revealed that while the strain BT-209 showed higher sensitivity than B. thuringiensis subsp. kurstaki HD-1 to ampicillin, bacitracin, chlortetracycline, gentamycin, neomycin, penicillin G, tetracycline and tobramycin, it was more resistant to methicillin than B. thuringiensis subsp. kurstaki HD-1. The $\delta$-endotoxin crystal of strain BT-209 consisted of three proteins with apparent molecular weights of appoximately 148, 135 and 62 kDa on a 10% SDS-PAGE. The strain BT-209 had at least eight different plasmids with sizes of 4.1, 5.2, 6.3, 8.6, 14.6, 24.5, 67.6 and 77.6 Kb. The strain BT-209 showed strong lethalities of 70% and 87% against Bombyx mori and Hyphantria cunea larvae. at 72 h, respectively.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1981년도 제15차 학술대회연제순서 및 초록
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• pp.39.2-39
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• 1981

지난 약 반세기에 걸쳐서 Penicillin을 비롯하여 많은 항생제 및 화학요법제의 등장으로 급성 혹은 만성중이염의 치료에 많은 도움을 주었다. 그런데, 근래에 이같은 약제의 무절제한 사용 및 오용으로 인한 내성균의 출현과 균교대현상 등으로 치료에 곤란을 겪고 있는 실정이다. 연자 등은 만성적인 이루를 주소로 하여 내원한 만성중이염 환자 50례를 대상으로 무균상태하에서 이루를 채취하여 균을 배양 및 동정하였으며 pipemidicacid를 비롯한 여러 항생제에 대한 감수성검사를 시행하였다. 그결과 중이염환자 50례에서 staphylococcus aureus 및 epidermidis, pseudomonas aeruginosa 및 putrefasciens, proteus mirabilis, $\alpha$-hemolytic streptococcus, klebsiella pneumonia, unproved gramnegative rod, serratia marcescens, corynebacterium이 검출되었으며, pipemidic acid를 투여하여 pseudomonas와 proteus에 의한 중이염에 좋은 치료효과를 보였으며 staphylococcus를 비롯한 다른 감염균에도 괄목할만한 치료효과를 나타냈다. Pipemidic acid는 piromidic acid의 새로운 유도체로서 gram양성균 뿐만 아니라 pseudomonas를 포함한 gram음성체에도 저명한 항균효과가 있음을 알 수 있었다.

• 한국환경과학회지
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• 제21권3호
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• pp.267-276
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• 2012

Spaceflights results in the reduction of immune status of human beings and increase in the virulence of microorganisms, especially gram negative bacteria. The growth of Klebsiella pneumoniae is enhanced by catecholamines and during spaceflight, elevation in the levels of cortisols occurs. So it is necessary to know the changes in physiology, virulence, antibiotic resistance and gene expression of K. pneumoniae under microgravity conditions. The present study was undertaken to study effect of simulated microgravity on growth, morphology, antibiotic resistance and cross stress resistance of K. pneumoniae to various stresses. The susceptibility of simulated microgravity grown K. pneumoniae to ampicillin, penicillin, streptomycin, kanamycin, hygromycin and rifampicin were evaluated. The growth of bacteria was found to be fast compared with normal gravity grown bacteria and no significant changes in the antibiotic resistance were found. The bacteria cultured under microgravity conferred cross stress resistance to acid, temperature and osmotic stress higher than the normal gravity cultured bacteria but the vice versa was found in case of oxidative stress.