• Title/Summary/Keyword: pencicillin G acylase

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Escherichia coli GroEL was Induced by the Expression of the Cloned Bacillus megaterium ATCC14945 Pencillin G Acylase Gene (클론된 Bacillus megaterium ATCC14945의 페니실린 지 아실라제의 발현에 따른 대장균에서의 GroEL의 유도 생산)

  • Hyun, Kang Joo;Kim, Sung Sun;Yoo, Ook Joon
    • Korean Journal of Microbiology
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    • v.30 no.6
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    • pp.421-424
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    • 1992

  • Escherichia coli JM83 harboring penicilin G acylase gene of Bacillus megaterium ATCC14945 produced a protein in large amount (>20% of the total protein). The protein was identified as GroEL, one of the E. coli heat shock protein, by N-terminal amino acid sequence analysis. It was found that GroEL was induced by the expressed foreign penicilin G acylase at both 27 and $37^{\circ}C$.

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