• Korean Journal of Agricultural Science
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• v.50 no.2
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• pp.241-247
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• 2023

Erwinia amylovora , which causes fire blight disease on apple and pear trees, is one of the most important phytopathogens because of its devastating impact. Currently, the only way to effectively control fire blight disease is through the use of antibiotics such as streptomycin, kasugamycin, or oxytetracycline. However, problems with the occurrence of resistant strains due to the overuse of antibiotics are constantly being raised. It is therefore necessary to develop novel disease control methods through an advanced understanding of the pathogenesis mechanism of E. amylovora . To better understand the pathogenesis of E. amylovora , we investigated unknown virulence factors by random mutagenesis and screening. Random mutants were generated by Tn5 transposon insertion, and the pathogenicity of the mutants was assessed by inoculation of the mutants on apple fruitlets. A total of 17 avirulent mutants were found through screening of 960 random mutants. Among them, 14 mutants were already reported as non-pathogenic strains, while three mutants, TS3128_M2899 (ΔSUFU ), TS3128_M2939 (ΔwcaG ), and TS3128_M3747 (ΔrecB ), were not reported. Further study of the association between E. amylovora pathogenicity and these 3 novel genes may provide new insight into the development of control methods for fire blight disease.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.12 no.1
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• pp.254-267
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• 1999

Based on the research of cataract, glaucoma, bluish glaucoma, we have abtained the following results. 1. Main etiologies of the cataract are deficiency of the liver and kidneys, main symptoms are no nebula, no pain, no tear and sometimes light blue and white vision appear. 2. Main etiologies of glaucoma are receives heat from the liver, stagnation of the liver and gall bladder etc, main symptoms are both forehead corner pull each ether, pain from eye ball to nasal septum, headache, extending pain of the eyes, sometimes red and white flower vision appear, etc. 3. main etiologies of the bluish glaucoma are melancholy, wrath, stagnation of the liver, internal damage, flaming up fire of the phlegm, deficiency of the liver, deficiency of blood, etc, main symptoms are numbness, visual field constriction, blindness, reduce of visual capacity, blue flower vision sometimes appear, etc. 4. Treatments are mainly uses nourish Um(陰) to reduce pathogenic fire, relieve stagnancy of Ki(氣) of the liver, nourish Um(陰) of the liver and kidneys, etc, acupuncture points are mostly contended arounded eyes such as BL10)(天柱), GV16(風府), LR3)(太衝), HT5)(通里) and in the case of nauses and vomitting PC6)(內關, ST36)(足三里) are included in addition to the above.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.10 no.1
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• pp.209-246
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• 1997

In the literatual study on the Ecthyma, the results were as follows. 1. The pathogenic factors of Ecthyma is divided three parts. One is exogenous pathogenic factors which including the wind evil, wind heat and toxic material produced by wetness evil. Another is endogenous pathogenic factors which including the declination of kidney-yang, impairment of the liver and kidney, the lower classes of yin-fire, accumulation of wetness-heat in the spleen asthenia, impairment of the liver and kidney, wetness-heat of three yang, asthenic heat-syndrome of three yin. The other is pathogenic factors neither endogenous nor exogenous which including the food and living, uncontrol sexual excess, anxiety and angry, injury of skin, injury of insects and animals. 2. Five viscera which was concerned with Ecthyma are liver, spleen and kidney. 3. Frequent region of Ecthyma are S-36(足三里) and C-7(陰交). External Ecthyma was rose to wetness-heat of three yang channel that cured easily. Internal Ecthyma was rose to asthenic heat-syndrome of three yin channel that cured hardly. 4. In the frequency of prescription, the most numerous prescription is Bojungikgitang(補中益氣湯) and the next are Kyukgigo(隔紙膏) and Yukmijihwanghwan(六味地滉丸). 5. In the frequency of medicine, the most medicine is Calomelas(輕粉) which included Hydrargyrum(水銀) and the next are Olibanum(乳香) and Resina Commiphorae Myrrhae(沒藥) which regulating vital energy and pain control medicine used that in order to destroy insects and remove polson. 6. In classification of the medical action, medicine of clearing away summer-heat and heat evil and activating blood circulation to dissipate blood stasis used to be very busy which in order to remove the disorder of vital energy for virulent heat-evil. 7. In classification of four characters, the most part is warm medicine, the next are cold and cool medicine and there is a few that is hot medicine. 8. In classification of five tastes, the most numerous tastes are bitter and acrid, the next are sweet, salty and sour tastes. 9. In classification of virulence of medicine, the most part is non-toxic, the next are weakly and deadly poison. 10. In classification of channel distribution, the most is the medicine that belongs to liver channel, the next are the lung, spleen, stomach and kidney channel.

• The Plant Pathology Journal
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• v.38 no.3
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• pp.194-202
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• 2022

Erwinia amylovora and Erwinia pyrifoliae cause fire blight and black-shoot blight, respectively, in apples and pears. E. pyrifoliae is less pathogenic and has a narrower host range than that of E. amylovora. Fire blight and black-shoot blight exhibit similar symptoms, making it difficult to distinguish one bacterial disease from the other. Molecular tools that differentiate fire blight from black-shoot blight could guide in the implementation of appropriate management strategies to control both diseases. In this study, a primer set was developed to detect and distinguish E. amylovora from E. pyrifoliae by conventional polymerase chain reaction (PCR). The primers produced amplicons of different sizes that were specific to each bacterial species. PCR products from E. amylovora and E. pyrifoliae cells at concentrations of 10⁴ cfu/ml and 10⁷ cfu/ml, respectively, were amplified, which demonstrated sufficient primer detection sensitivity. This primer set provides a simple molecular tool to distinguish between two types of bacterial diseases with similar symptoms.

• Journal of Haehwa Medicine
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• v.11 no.1
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• pp.163-177
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• 2002

This study has been carried out to investigate the cause and etiology of vertigo by referring to 47 literatures. The results were as follows ; 1. Vertigo is classified in Oriental Medicine into Exess Symptom-Complex(實證) and Deficiency Symptom-Complex(虛證). Exess Symptom-Complex(實證) is caused by flare-up the fire of the liver(肝陽上亢), phlegm-heat(痰火) and exogenous pathogenic factors(外邪). Deficiency Symptom-Complex(虛證) is caused by insufficiency of the yin of the kidney(腎陰不足) and deficiency of qi and blood(氣血虛). 2. The principles of vertigo treatments are pyongganjamyang(平肝潛陽), sohwasigpung(消火息風), boiggihyul(補益氣血), geonunbiui(建運脾胃), boigsinjeong(補益腎精), chungyangnoisu(充養腦髓), joseubgeodam(燥濕祛痰), geonblhwaui(建脾和胃). 3. Various Needling Treatment Methods of vertigo in the recent Oriental Medicine are presented such as Filiform Needle(鍼刺療法), Auricular Acupuncture Therapy(耳鍼療法), Scalp Acupuncture Therapy(頭鍼療法), Cataneous Needle Therapy(皮膚鍼療法), Aqua Acupuncture Therapy(水鍼療法), etc.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.11 no.1
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• pp.99-140
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• 1998

In the literatual studies on the suppurative osteomylitis(附骨疽), the results were as follows. Suppurative osteomylitis(附骨疽) is called 'Mudujur(無頭疽)', is concerned with Pyogenic osteomyelitis in western medicine. The pathogenic factors of Suppurative osteomylitis were caused by exposing to wind-cold-wetness evil, or body weakness, or wound a bodily injury caused by physical means. The symptoms of Suppurative osteomylitis was alternating episodes of chills and fever, pain on buttock or thigh in onset. The differential diagnosis of Suppurative osteomylitis was divided three parts. One was the stagnation of virulent fire-evil. Another was the deficiency of both vital energy & blood. The other was wind-cold-wetness evil. The treatments of Suppurative osteomylitis was clearing away heat-evil and wetness evil, removing blood stasis and promoting meridian. In the frequency of prescription, the most numerous prescription were Osintang(五神湯) and Hwangyunhaidoktang(黃連解毒湯), the next were Neitakganghwaltang(內托羌活湯), Taibangpungtang(大防風湯) etc.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.12 no.1
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• pp.295-312
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• 1999

A literature study on the nasal stuffiness, the results are as follows. 1. The pathogenic factors nasal stuffiness are wind-cold, cold, fire-heat, heart & lung disease, deficiency of lung-energy and spleen & stomach disease caused by internal damage. 2. The method on the external treatment of nasal stuffiness are obstructive method, blowing method and pouring method, obstructive method used many. 3. The method on the internal treatment of nasal stuffiness are diaphoretic therapy, clearing away heat & toxic materials and warming the lung & invigorating the spleen. 4. You-taek-tong-gue-tang is used in nasal stuffness. 5. Herba Asari, Radix Angelicae Dahuricae, Flos Magnaliae, Fructus Xanthii, Rhizama Acori Graminei, Herba Menthae, Zanthoxyli Fructus and Spina Gleditsiae are used in nasal stuffness.

• Journal of Korean Medical classics
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• v.21 no.1
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• pp.235-255
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• 2008

Byeollyangchamsa has been a key factor in formation of Dongwon(東垣)'s theory on internal injuries. The majority regarded Byeollyangchamsa as just epidemic disease, while Dongwon(東垣) regarded it as internal injuries by improper diet and overstrain. However, an analysis of Dongwon(東垣)'s internal injury syndrome revealed that it was a kind of external affections caused by damp-heat. In particular, heat rather than dampness have developed as the disease got worse. Therefore It can be regarded as the serious syndrome to show the impairment of Gi(氣), blood, fluids and humors for pathogenic fire.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.4
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• pp.642-648
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• 2002

The Dryness and the Dampness is the congential nature. the Water and Fire is the acquired action. the skin is related to the physiological function of the lung, so the skin is the domain of the lung. the lung belongs to the dryness-metal(燥金). And the Dryness and the Dampness in the six pathogenic factors exert a important pathogenic influence upon the skin. Psoriasis is a chronic skin condition charactered by red, silver scaly patches in the affected areas, it is caused by excessive growth of the top layer of the skin(epidermis). Scalp psoriasis affects at least two thirds people who have psoriasis. Generally, the scalp will have plaque psoriasis characterized by elevated, inflamed, lesions covered with silvery white scales. The lesion of psoriasis is head, elbow and knee that are yang-channels in Oriental medicine. The syndrome falls with in the purview of the dryness-metal(燥金). In time, psoriasis takes a turn for the worse in the winter season. In space, many of people in comparison to the average in the world have psoriasis in high latitude that is dry and cold, for instance, the Scandinavian peninsula. Deficiency of yin-fluid and blood course often the dryness-syndrome, for instance, psoriasis, etc. So psoriasis is one of the dryness-syndrome in Oriental medicine.

• Herbal Formula Science
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• v.12 no.1
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• pp.105-121
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• 2004

This report describes the studies as follows through the research into the data of Fructus Forsythiae main blended prescriptions from Dongeuybogam. Through investigation on prescriptions from Dongeuybogam with Fructus Forsythiae as an ingredient, the following conclusions were reached. 1. All kinds of infection in a boil and the malignant tumor recorded the largest number of clinical frequency of the prescriptions in therapeutic use when Fructus Forsythiae was taken as a principle medicine. 2. The prescriptions are compounded with Fructus Forsythiae as a principle medicine can apply to eye disease, nose and ear disease, throat disease, trauma, the malignant tumor, skin disease, dental disease and heat pathogenic disease. 3. Various pathogenic factors such as wind-heat, heat, fire, wind, qi, poison, cold are put to practical use. 4. The dosage of Fructus Forsythiae is 2bun(about 0.75 gram) to 3jeon(about 11.25 gram), however 1joen(about 3.75 gram) has been taken the most for clinical application. 5. With examination the formulae of presciptions containing Fructus Forsythiae, I found the basic prescriptions as well as the medicines are in conjunction with Fructus Forsythiae and the theoretic grounds of formula in combining Fructus Forsythiae as a group of medicine for prescription through this research.