• Korean Journal of Agricultural Science
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• v.44 no.2
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• pp.181-187
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• 2017

The use of practical management factors to maximize pig health improvement cannot guarantee freedom from diseases. Moreover, because of health safety concerns, the use of antibiotics has been restricted in livestock, including pigs. Therefore, the swine industry has been looking for various alternatives to antibiotics to improve pig's health and performance. Clay is a dietary factor generally accepted for improving pig health. It is a naturally occurring material and is primarily composed of fine-grained minerals. It has a specific structure with polar attraction. Because of this structure, clay has the ability to lose or gain water reversibly. In addition, clay has beneficial physiological activities. First, clay has anti-diarrheic and antibacterial effects by penetrating the cell wall of bacteria or inhibiting their metabolism. Second, it can protect the intestinal tract by absorbing toxins, bacteria, or even viruses. When added to the diet, clay has also been known to bind some mycotoxins, which are toxic secondary metabolites produced by fungi, namely in cereal grains. Those beneficial effects of clay can improve pigs' health and performance by reducing pathogenic bacteria, especially pathogenic Escherichia coli, in the intestinal tract. Therefore, it is suggested that clay has a remarkable potential as an antibiotics alternative.

• Archives of Pharmacal Research
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• v.29 no.3
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• pp.249-255
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• 2006

Ability of iron acquisition of pathogenic microorganisms functions as a virulence factor. Candida albicans, a fungal pathogen that requires iron for growth, is susceptible to growth retardation by high-affinity iron binding proteins such as transferrin. Recently, we reported that C. albicans could utilize the heme as a part of heme-containing proteins dissociated by heme oxygenase, CaHMX1. In search of another pathway that C. albicans can use to bypass the growth regulation produced by iron limitation, this present study examined utilization of non-candidal siderophores such as Desferal and rhodotorulic acid (RA) for acquisition of inorganic iron by the fungus. C. albicans secreting no siderophores was cultured in iron-free (pretreated with apotransferrin for 24 h) (culture medium). Once growth of the yeast reached stasis from iron starvation, a siderophore was added to the culture media. Results showed that cultures containing apotransferrin within a dialysis membrane recovered growth to the level of untreated controls, whereas C. albicans yeast cells in direct contact with soluble iron-free (apo) transferrin recovered growth only partially. When static growth from iron limitation was reached, the addition of siderophore-apotransferrin complex to culture medium also permitted the yeast to recover growth from apotransferrin growth regulation. All the data show that C. albicans can utilize the non-candidal siderophores for iron acquisition under transferrin regulation as can pathogenic bacteria.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.13 no.1
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• pp.209-236
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• 2000

This study has been carried out to investigate pathogenic factor and medicine of ja-shi(자시) by referring to literatures. The results were obtained as follows. 1. In oriental medicine, Chashi(차시), Hamshichang(함시창), Hamshi(함시), Jabal(자발), Shichang(시창), Nojaon(로자온), Hamaon(하마온), Jokshiong(적시옹), Hamshidok(함사독), Tabshijong(탑사종), Shijong(시종) were the other names of ja-shi(자시). 2. The pathogenic factors of ja-shi(자시) are wind-heat, wetness-heat, Kihyeolungche(기혈응체), stomach-heat. wetness, wind wetness, and liking greasy and sweet food. 3. In the internal therapy of ja-shi(자시), Bojesodokum(普濟消毒飮), Sasunchunglyangum(四順淸凉飮), Takljsodoksan(托裏消毒散), Shihogalguntang(柴胡葛根湯), Sungsohwan(醒消丸), Gamisodokum(加味消毒飮) and etc. were used. And Radix glycyrrhizae(甘草), Fructus forsythiae(連翹), Radix angelicae gogantis(當歸), Radix scutellariae(황기), Radix bupleuri(柴胡), Radix p]atycodi(桔梗), Radix Ledebounellae(防風), Frustus arctii(牛蒡子), Rhizoma cimicifugae(升麻) and ete. were used as medicine. 4. In the external therapy of ja-shi(자시), Youigumhwangsan(如意金黃散), Okiogo(玉露膏), Igumsan(二金散), Gumhwangsan(金黃膏), Nungo(嫩膏) and etc. were used. And Radix angelicae dahuricae(白芷), Cortex phellodendri(黃栢), Rhizoma rhei(大黃), Indigo maturalis(靑黛), Radix glycyrrhizae(甘草) and etc. were used as medicine.

• Journal of Korean Medical classics
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• v.30 no.3
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• pp.181-195
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• 2017

Objectives : This paper contemplates how the special features found in Moyuan as revealed in Wenyilun has continued from the Moyuan concepts of past includig Huangdineinjing. The author of this paper attempted to distinguish common and different points between the two, and how these features changed in the following generations. Methods : The concept of Moyuan as revealed in Huangdineinjing is first reviewed before laying out the concepts of Moyuan in Wenyilun by different categories. These two concepts are then compared. A Chinese medicine literature database is used in continuing this study, and materials are selected with relation to the special features of Moyuan. Results : The special feature of half-exterior half-interior found in the Moyuan in Wenyilun is similar with that in Neijing in that they both thought Moyuan existed between the stomach, the lungs, and the five organs. The concealability of Moyuan is similar with the explanation of Moyuan in Suwen nuelun, and it is related with the story of Gaohuang in Chunqiu Zuozhuan. Compared to the fact that the concept of Moyuan in Neijing is explained as that pathogenic factor enters the surface of the body in sequence, the concept of Moyuan in Wenyilun is conveyed in that the pathogenic factor conceals itself first before being divided into exterior and interior. Moyuan is also related to upright qi, and Gaohuang-yuan: origin of Gaohuang, too, is connected Shenjiandongqi: the energy that moves between the two kidnets, and also the triple burner. Conclusions : The concepts of "Half-exterior Half-interior", concealability, and "divide it into several places in half-exterior", and upright qi contained within the Moyuan in Wenyilun are studied for their origins, and this effort is anticipated to be greatly helpful in both understanding the historical contexts of Wuyouke Wenyilun and conducting studies on clinical application of Moyuan theory.

• Journal of Korean Medical classics
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• v.23 no.1
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• pp.257-277
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• 2010

The purpose of this article is to oversee the theory of Chimibyeong(治未病). The aim of Chimibyeong theory is to prevent disease from occuring, worsening, being delivered and so on. In many books, the word Chimibyeong was used as an alternative name for Yangsaeng(養生) or used as a term of preventing a disease or used as a word meaning treating disease in the early stage. But after the period of "Nangyeong(難經)", people extend the meaning of Chimibyeong to all stages of a disease covering the healthy stage, the early stage, the progressive, and the recovery stage of a disease. Especially in urgent cases when pathogenic factor[邪氣] attacks patient to critical situations, it is also regarded as one way of Chimibyeong to repel pathogenic factor out as soon as possible with proper medicines. These days people suffer from increasing mental stress, lack of rest and human relationships, environmental pollution and chronic diseases and so on, and the situation will grow worse. Many governments employ the health policy where preventing disease takes the highest priority because by doing that they can save a huge budget and minimize the economic and social disorder. In this circumstances the idea of pursuing prevention in the theory of Chimibyeong will help people to maintain healthy conditions.

• Journal of Biomedical and Translational Research
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• v.19 no.4
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• pp.65-72
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• 2018

The purpose of this study was to investigate probiotic characteristics and immune enhancing effects of Bifidobacterium (B.) longum KBB1-26 and BIF-4, B. breve KBB5-22 isolated from human intestine for probiotic use in humans and animals. We measured acid, bile and heat tolerance, antimicrobial activity against pathogenic bacteria, Escherichia (E.) coli, Salmonella (S.) Enteritidis, Staphylococcus (S.) aureus, and Listeria (L.) monocytogenes. Immune enhancing effects of B. longum and B. breve were investigated by measuring nitric oxide (NO), nuclear factor ($NF-{\kappa}b$), $interleukin-1{\beta}$ ($IL-1{\beta}$), interleukin-6 (IL-6), interleukin-12 (IL-12) and tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) in RAW 264.7 cells or RAW BLUE cells. B. longum KBB1-26 was survived at pH 2.0. B. longum KBB1-26 and BIF-4, B. breve KBB5-22 also showed tolerance to 0.3% of oxgall bile salt. B. longum KBB1-26 was able to survive at $70^{\circ}C$ and $80^{\circ}C$ for 20 min. KBB1-26 showed the antimicrobial inhibition zone to pathogenic bacteria such as E. coli (12 mm), S. Enteritidis (14 mm), S. aureus (14 mm) and L. monocytogenes (41 mm). The production of NO ($4.5{\pm}0.00{\mu}M/mL$) and $IL-1{\beta}$ ($39.7{\pm}0.55pg/mL$) of KBB1-26 significantly higher than BIF-4 and KBB5-22, respectively. In addition, KBB1-26 and KBB5-22 induce the production of high level of $TNF-{\alpha}$ and IL-6 in macrophages. Collectively, B. longum KBB1-26 have acid, bile, heat tolerance, antimicrobial activity and immune enhancing effects. These results suggest that KBB1-26 can be used as probiotics for humans and animals.

• Journal of Environmental Science International
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• v.11 no.12
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• pp.1281-1289
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• 2002

This study was conducted to evaluate water quality in the Keum River using multivariate analysis. The analysis data in Keum river made use of surveyed data by the ministry of environment from January 1994 to December 2001. Thirteen water quality parameter were determined on each sample. The results was summarized as follow; Water quality in the Keum River could be explained up to 71.39% by four factors which were included in loading of organic matter and nutrients by the tributaries (32.88%), seasonal variation (16.09%), loading of pathogenic bacteria by domestic sewage of Gapcheon (13.39%) and internal metabolism in estuary as lakes(9.03%). For spatial variation of factor score, four group was classified by each factor characterization. Station 1 and 2 was influenced by Daechung dam, station 3 was affected by domestic sewage of Gapcheon, station 10~12 was affected by estuary dyke and the rest station. The result of cluster analysis by station was classified into four group that has different water quality characteristics. In monthly cluster analysis, three group was classified according to seasonal characteristic. Also, in yearly cluster analysis, three group was classified. It is necessary to control the pollutant loadings by Gapcheon inflow domestic sewage in Daejeon city for the sake of water quality management of Keum river.

• Korean Journal of Veterinary Service
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• v.44 no.4
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• pp.271-281
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• 2021

Pathogenic Escherichia coli (E. coli) is one among the most important agents of diarrhea in calves. From January to December 2021, 108 isolates from feces of calves with diarrhea were investigated for enterotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC), and enteroinvasive E. coli (EIEC) using real-time PCR. In addition, the genes for F5, F17 and F41 fimbriae were detected by PCR. The most frequently isolated pathotypes were EPEC/STEC (29 isolates), and ETEC/EPEC/STEC (29 isolates). ETEC/EPEC, and ETEC/STEC were also found in 10 isolates. EPEC, STEC, and ETEC were detected in 13, 11, and 6 respectively. EAEC, and EIEC was not detected. Antimicrobial resistance test was carried out by agar disc diffusion method with 14 antimicrobials. Among 108 pathogenic E. coli isolates, 107 isolates were resistant to at least one of 14 antibiotics used in this study, 99 (91.7%) were resistant to two or more antimicrobials, and a single remarkable isolate was resistant to 14 antimicrobials. The isolates were primarily resistant to penicillins, streptomycin, tetracycline, ceftiofur, Trimethoprim/sulfamethoxazole, Kanamycin, and Ciprofloxacin. The high rate of resistance in pathogenic E. coli, sometimes to multiple drugs, may complicate future options for treating human infections. These results may bu used for diagnosis and therpeitic purposes in calves with diarrhea.

• Journal of Microbiology and Biotechnology
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• v.18 no.7
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• pp.1317-1325
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• 2008

Viral safety is an important prerequisite for clinical preparations of plasma-derived pharmaceuticals. One potential way to increase the safety of therapeutic biological products is the use of a virus-retentive filter. In order to increase the viral safety of human antihemophilic factor IX, particularly in regard to non-enveloped viruses, a virus removal process using a polyvinylidene fluoride membrane filter (Viresolve NFP) has been optimized. The most critical factor affecting the filtration efficiency was operating pH and the optimum pH was 6 or 7. Flow rate increased with increasing operating pressure and temperature. Recovery yield in the optimized production-scale process was 96%. No substantial changes were observed in the physical and biochemical characteristics of the filtered factor IX in comparison with those before filtration. A 47-mm disk membrane filter was used to simulate the process performance of the production-scale cartridges and to test if it could remove several experimental model viruses for human pathogenic viruses, including human hepatitis A virus (HAV), porcine parvovirus (PPV), murine encephalomyocarditis virus (EMCV), human immunodeficiency virus type 1 (HIV), bovine viral diarrhea virus (BVDV), and bovine herpes virus (BHV). Non-enveloped viruses (HAV, PPV, and EMCV) as well as enveloped viruses (HIV, BVDV, and BHV) were completely removed during filtration. The log reduction factors achieved were $\geq$6.12 for HAV, $\geq$4.28 for PPV, $\geq$5.33 for EMCV, $\geq$5.51 for HIV, $\geq$5.17 for BVDV, and $\geq$5.75 for BHV. These results indicate that the virus filtration process successfully improved the viral safety of factor IX.

• Journal of Environmental Science International
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• v.13 no.3
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• pp.233-244
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• 2004

This study was conducted to evaluate water quality in the Mankyung River using multivariate analysis. The analysis data which was surveyed from January 1996 to December 2002 in Mankyung river was aquired by the ministry of environment. Twelve water quality parameters were determined on each survey. The results were summarized as follow; Water quality in the Mankyung River could be explained up to 74.90% by four factors which were included in loading of organic matter and nutrients by the tributaries(43.28%), seasonal variation(10.40%), loading of pathogenic bacteria by domestic sewage of Gapcheon (12.41%) and internal metabolism in river(8.81%). The result of cluster analysis by station was classified into three group that has different water quality characteristics. Especially, Iksan river was appeared to considerable water quality characteristics against other station. In monthly cluster analysis, three group was classified by seasonal characteristics. Also, in yearly cluster analysis, three group was classified. It is necessary to control the pollutant loadings by domestic sewage and livestock waste for water quality management of Mankyung river.