• 농업과학연구
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• 제46권4호
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• pp.981-989
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• 2019

Coffee is a popular beverage worldwide, and the scale of consumption is growing rapidly. Many studies have shown that increased coffee consumption has various effects on human health, including beneficial effects on liver diseases, clinical type 2 diabetes, and Parkinson's disease. However, the influences of coffee or caffeine (a component of coffee) on the gut microbiota have not been examined in detail. Here, we tested whether caffeine could alter the antimicrobial activity of L. casei against E. coli. Interestingly, we found that treatment with 0.3 mg/mL caffeine increased the antimicrobial activity of L. casei against E. coli. This activity was not associated with the release of lactic acid but did appear to be related to a heat-labile factor present in the L. casei culture supernatant. Our analyses suggest that the putative antimicrobial factor found in the culture supernatant of L. casei treated with caffeine may be bacteriocin. Taken together, our results suggest that caffeine, which is an ingredient of coffee, increases the antimicrobial activity of L. casei against E. coli through the enhanced production of bacteriocin. These findings also suggest that coffee consumption affects the ability of beneficial bacteria to decrease pathogenic bacteria and/or prevent the progression of bacterial infection-associated diseases in the gut.

• 대한미생물학회지
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• 제35권4호
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• pp.317-324
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• 2000

Candida albicans is one of the most frequently isolated fungal pathogens in human. Recently, the prevalence of candida infection has markedly increased, partially due to the increase of immunocompromised hosts. Proposed virulence factors of the pathogenic Candida are the ability to form hyphae to adhere to epithelial cell surfaces, and to secrete acid proteinases and phospholipases. We measured the relative cell surface hydrophobicity (CSH) and the ability of proteinase production (PROT), phospholipase production (PLase), adherence to host epithelium (ADH), and hyphal transition (Germ). The relative risk of virulence factors was analyzed by lethality test in murine model of hematogeneously disseminated candidal infection. According to Cox's proportional hazard analysis, the statistically significant virulence factors were PROT, ADH, and CSH. PROT was the highest risk factor of them. To evaluate the applicability for the diagnosis and treatment of Candidiasis, we examined the protective effect of the active and passive immunizations with the materials purified from virulence factors and antibodies to them in Candia-infected mice model. The mean survival times of active and passive immunized groups were slightly longer than those of non-immunized groups.

• 한국어병학회지
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• 제21권3호
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• pp.247-257
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• 2008

To understand the activity of non-specific defence factors in cultured Sebastes, the antibacterial effect of the serum, skin mucus and homogenate of various organs from cultured oblong rockfish (Sebastes oblongus) and rockfish(Sebastes schlegeli) against pathogenic bacteria, Aeromonas hydrophila, Edwardsiella tarda, Vibrio anguillarum, and Streptococcus sp. was compared with that of flounder(Paralichthys olivaceus) and seabass(Leteolabrax japonicus). And the activities of proteolytic enzyme, chitinolytic enzyme and haemolycin as non-specific defence factor were investigated on the oblong rockfish and rockfish. Samples from oblong rockfish showed the highest antibacterial activity by lysoplate assay on agar plate mixed with pathogens, followed in descending order by rockfish, seabass, and flounder. Turbidimetric assay was carried to evaluate the lysozyme activity of fish samples against lyophilized cells of Micrococcus lysodeiktikus. The serum, kidney, liver, stomach, intestine and eyeball of oblong rockfish and the mucus and gill of rockfish appeared to have the highest lysozyme activity among the fish strains investigated. All samples except skin mucus, liver, and eyeball of oblong rockfish and rockfish showed proteolytic enzyme activity. Chitinolytic enzyme activity was showed in random sampling and haemolytic activity was remarkable in oblong rockfish. Therefore, Sebastes strain was proved to have effective defense mechanisms based on the antibacterial activities, and lysozyme, proteolytic enzyme, chitinolytic enzyme, and haemolycin were considered to act as the non-specific defence factor of Sebastes.

• 한양메디칼리뷰
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• 제33권1호
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• pp.10-16
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• 2013

Follicular helper T cells (Tfh) play a significant role in providing T cell help to B cells during the germinal center reaction, where somatic hypermutation, affinity maturation, isotype class switching, and the differentiation of memory B cells and long-lived plasma cells occur. Antigen-specific T cells with IL-6 and IL-21 upregulate CXCR5, which is required for the migration of T cells into B cell follicles, where these T cells mature into Tfh. The surface markers including PD-1, ICOS, and CD40L play a significant role in providing T cell help to B cells. The upregulation of transcription factor Bcl-6 induces the expression of CXCR5, which is an important factor for Tfh differentiation, by inhibiting the expression of other lineage-specific transcription factors such as T-bet, GATA3, and RORγt. Surprisingly, recent evidence suggests that CD4 T cells already committed to Th1, Th2, and Th17 cells obtain flexibility in their differentiation programs by downregulating T-bet, GATA3, and RORγt, upregulating Bcl-6 and thus convert into Tfh. Limiting the numbers of Tfh within germinal centers is important in the regulation of the autoantibody production that is central to autoimmune diseases. Recently, it was revealed that the germinal center reaction and the size of the Tfh population are also regulated by thymus-derived follicular regulatory T cells (Tfr) expressing CXCR5 and Foxp3. Dysregulation of Tfh appears to be a pathogenic cause of autoimmune disease suggesting that tight regulation of Tfh and germinal center reaction by Tfr is essential for maintaining immune tolerance. Therefore, the balance between Tfh and Tfr appears to be a critical peripheral tolerance mechanism that can inhibit autoimmune disorders.

• 생명과학회지
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• 제16권4호
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• pp.605-611
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• 2006

양식 산업의 문제점인 어병균을 방제하기 위한 생물 전구체의 개발을 목적으로 해산어의 내장 추출물에서 어병균인 Vibrio anguillarum의 생육을 저해하는 새로운 균주를 분리, 동정하여 Bacillus amyloliquefaciens H41으로 명명하고 생육특성 및 생육저해인자 생성 조건을 검토하였다. 분리 균의 생육 최적 조건은 기본배지로 1% peptone, 질소원으로 1.5% yeast extract, 탄소원으로 1% sucrose, 0.5% NaCl, 금속이온으로는 0.05% $MgSO_4{\cdot}7H_20$이었으며 pH는 7.0-8.0, 온도는 $28-35^{\circ}C$에서 20시간의 진탕배양이었다. V. anguillarum 생육 저해물질은 B. amyloliquefaciens H41의 배양 상등 액에서 볼 수 있었는데 paper-disk 법으로 투명환의 생성여부로 확인하였다. 분리 균에 의한 생육저해 물질 생산 최적 조건은 1% peptone, 1.5% yeast extract, 1% NaCl, 0.05% $MgSO_4{\cdot}7H_20$, 1%의 sucrose이었으며 최적 pH는 7.5, 최적온도는 $35^{\circ}C$이었다. 분리 균은 최적 배양조건하에서 저해물질을 배양 후 16시간부터 24시간대에 가장 높은 활성을 나타내었다. 저해 활성반응은 분리 균에서는 저해 활성을 나타내었으나 B. amyloliquefaciens KCTC1724 표준균주에는 저해활성을 나타나지 않는 대조를 보였다. 또한 분리 균에 의해 생산된 저해물질은 V. anguillarum 에는 생육 억제 효과를 나타내었으나 다른 인체 병원성 Vibrio에는 전혀 영향을 미치지 않는 것으로 나타나 균주 선택성이 특이적이었다.

• 한국산학기술학회논문지
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• 제20권1호
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• pp.189-199
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• 2019

본 연구의 목적은 축산차량의 이동이 빈번하게 일어나는 공간적 범위(방역권역)를 도출하는 것을 목적으로 한다. 이를 위하여 본 연구는 KAHIS에서 제공하는 축산차량의 시설 진입자료 중에서 6개 시점의 자료를 활용하였다. 이 자료를 축산시설간의 차량이동자료로 변환하고, 이를 행정구역단위로 취합하여 행정구역간의 OD자료를 구축하였다. 구축된 OD 자료를 활용한 R-mode 요인분석을 실시하여 동일요인으로 추출된 지역을 하나의 권역으로 판단하였다. 분석결과를 정리하면 다음과 같다. 첫째, 6개 시점 자료의 요인분석을 실시한 결과 16개~18개의 요인이 도출되었으며, 도출된 요인은 전체 분산의 63~68%를 설명하였다. 두 번째, 도출된 요인을 바탕으로 지역을 구분한 결과, 전남남해안지역, 전남지역, 전북지역, 충남서해안지역, 경남지역, 경북북부지역, 영남동해안지역은 시점에 따른 변화가 적은 안정적인 지역으로 나타났다. 반면, 충북지역, 강원도지역, 수도권지역은 상대적으로 변화가 큰 지역이다. 세 번째, 6개 시점의 자료를 종합하여 강원권역, 경기남부권역, 경기북부권역, 경남권역, 경북남부권역, 경북북부권역, 남해안권역, 대전권역, 동해안권역, 전남권역, 전북권역, 충남권역, 충북권역의 13개 권역을 도출하였다.

• 한국산학기술학회논문지
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• 제13권11호
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• pp.5247-5253
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• 2012

본 연구는 식혜와 액상 커피의 제조 공정중 HACCP system을 이용하여 한계 기준 설정을 위한 목적으로 실시하였다. 살균공정의 한계 기준 설정은 충북 진천시 소재의 P사에서 약 30일 (2012년 4월 1~30일) 동안 살균온도와 시간을 측정하였다. 그 결과, 멸균전에는 식혜와 액상 커피에서 미생물이 검출되었다. 반면에 식혜(238mL Can, 500mL and 300mL PP, 1.8L PP)에서 모든 미생물은 살균($121{\pm}1^{\circ}C$에서 $15{\pm}1$, $35{\pm}1$ and $45{\pm}1$분) 후에는 검출되지 않았고, 액상 커피도 살균($121{\pm}1^{\circ}C$, $20{\pm}1$분)후 검출되지 않았다. 가장 적당한 온도와 시간을 결정하기 위한 살균기 조건은 $121{\pm}1^{\circ}C$, $20{\pm}1$분이었다. 결론적으로, 살균공정은 유해미생물(일반세균, 대장균군, 병원성 미생물)를 예방, 감소 또는 제거할 수 있는 좋은 대안이 될 것이다. 따라서 품질 유지와 생물학적 안전성을 위한 살균 온도와 시간의 한계기준은$121{\pm}1^{\circ}C$에서 $20{\pm}1$분으로 설정하였다. 그리고 HACCP 계획은 살균 공정중 모니터링 방법과 모니터링 주기, 문제 해결 방법, 교육, 훈련, 기록 관리 등을 위하여 필요하여 이를 제안하고자 한다.

• 대한한의학원전학회지
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• 제11권1호
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• pp.676-718
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• 1998

After a comparative study on between Shanghanlun(傷寒論) and Sa-sang constitutional medicine(四象醫學) in Pathogenesis, I got a conclusion like this. Sa-sang consitutional medicine(四象醫學) changed the directions to the medicine in the center of personality from the medicine in the center of Shanghanlun(傷寒論)'s demonstration, devided the personality of people by the size of Organ system(臟腑) into 4types of Tae-eum, Yo-yang, Tae-yang, Yo-eum(太少陰陽), and explains all the course of physiology, pathology, diagnosis, therapy of the body on the point of constitutional view. Comparing the features of two medicines, Shanghanlun(傷寒論) and Sa-sang constitutional medicine(四象醫學), Shanghanlun(傷寒論) devided the diseases into the three type of eum-yang based on eight principal syndroms(八綱原理), in accordance with evidence of illness, pulse and studied the therapy, Shanghanlun(傷寒論) set up the basis of medicine which is based on overall of symptoms and signg(辨證論治醫學). Sa-sang constitutional medicine(四象醫學) distinguished the image(象) which is devided by the size of inherited Organ system(臟腑), refered to the symptom of diseases(病症) and decided the therapic directions. So Sa-sang constitutional medicine(四象醫學) gave more accuracy to the therapy by subdeviding the process of differenciation of case(辨證) into 2 steps of differenciation of image(辨象) and differenciation of case(辨證). In view of etiologic factor, Shanghanlun(傷寒論) regarded it as a invading of pathogenic factors(邪氣), so Shanghanlun(傷寒論) has the medical theory of pathogenic factors(邪氣). But Sa-sang constitutional medicine(四象醫學) regarded it as a disorder of the genuine energy(正氣)'s movements(升降緩速), so Sa-sang constitutional medicine(四象醫學) has the medical theory putting first of genuine energy(正氣). But Shanghanlun(傷寒論) also recognized the constitutional difference basically and Sa-sang consitutional medicine(四象醫學) devided the constitution into Tae-eum, Yo-yang, Tae-yang, Yo-eum(太少陰陽) and explained the food-air-fluid metabolism(飮食-氣液之氣病證) as cold-hot, cool-warm and devided the the symptom of diseases(病症) into the interior and the exterior(表裏) as the up-down, slow-fast movements of eum-yang and insufficiency and excessiveness is between them. In the end, Sa-sang constitutional medicine(四象醫學) has the theory of eight principal syndroms(八綱原理) faithfully which is the theory of differenciation of case(辨證理論) of Shanghanlun(傷寒論). Therefore Shanghanlun(傷寒論) made a lot of influence on originating Sa-sang constitutional medicine(四象醫學), Sa-sang constitutional medicine(四象醫學) is the theory which is based on existing medical theory including Shanghanlun(傷寒論) and composed the new medical theory to the constitutional point of view. Sa-sang constitutional medicine(四象醫學) enriched the medical theory and developed the clinical medicine so it has the historical value in the medicine.

• 한국축산식품학회지
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• 제30권5호
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• pp.764-772
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• 2010

Staphylococcus aureus lipase is regarded as a virulence factor. The response of lipase activity to various factors can provide important insights concerning the prevention of S. aureus during meat fermentation. This study was conducted to evaluate the main effects of nutrients used in culture media, and their combined effects on the inhibition of lipase activity and cell growth of pathogenic S. aureus SK1593 isolated from fermented pork meat. A Plackett-Burman design was used to evaluate the main effects of variables, including olive oil, soybean oil, grapeseed oil, sesame oil, $CuSO_4$, $MgCl_2$, $KNO_3$, $CaCl_2$, and KCl. Significant negative effects on lipase activity were detected with soybean oil, grapeseed oil, $KNO_3$, and $CaCl_2$. Additionally, these nutrients were further selected as variables for the investigation of their combined effect on lipase activity, via response surface methodology. In order to confirm the regression model, a situation that only inhibits lipase activity was simulated. The predicted lipase activity and cell growth of the simulated situation were 14.0 U/mL and $9.6\;{\log}_{10}$ (CFU/mL), respectively, and the estimated value of those in the same medium showed 15.14 U/mL and $9.4\;{\log}_{10}$(CFU/mL) respectively. The lipase activity of the simulated medium was inhibited approximately 5-fold as compared to the basal medium, but no significant differences in cell counts were noted to exist between the basal and simulated media. These results suggest that soybean oil, grapeseed oil, $KNO_3$, and $CaCl_2$ can be used to inhibit the growth of pathogenic S. aureus during the process of meat fermentation.

• 대한한의학방제학회지
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• 제19권2호
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• pp.161-178
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• 2011

동의보감(東醫寶鑑) 중(中) 향부자(香附子)가 주약(主藥)으로 배오(配伍)된 85개 처방(處方)에 대하여 자료조사(資料調査)를 한 바 다음과 같이 요약(要約)할 수 있었다. 1. 향부자(香附子)가 주약(主藥)으로 구성(構成)된 방제(方劑)의 치료영역(治療領域)에 따른 처방빈도는 부인문(婦人門)이 16.4%, 포문(胞門)이 15.2%, 기문(氣門)이 10.5%, 적취문(積聚門)이 8.1% 순으로 많은 빈도수를 차지하였으며, 그 밖에도 20개 치료영역(治療領域)에 사용되었음을 알 수 있었다. 2. 향부자(香附子)는 구사(求嗣), 월경부조(月經不調), 기울(氣鬱), 기통(氣痛), 적취(積聚)등의 병증(病症)에 가장 많이 활용되고 있었으며, 그밖에도 55개 병증에 사용하였다. 3. 향부자(香附子)가 주약(主藥)으로 사용된 처방(處方)의 병인병리(病因病理)는 제기울체(諸氣鬱滯), 칠정(七情), 담음(痰飮), 식상(食傷), 풍한(風寒)등의 병리(病理)가 많았다. 4. 향부자(香附子)가 주약(主藥)으로 사용된 처방(處方)의 1회 향부자(香附子) 사용량은 6리(厘)~6전(錢)까지 이며, 1전(錢)을 가장 많이 사용하였다. 5. 향부자(香附子)는 배오(配伍)되는 약물(藥物)과 처방에 따라 이기해울(理氣解鬱), 소간이기(疎肝理氣), 조경지통(調經止痛)등의 다양한 작용을 하는 것을 알 수 있으며, 향부자(香附子)가 주약(主藥)으로 사용된 처방(處方)의 기본방(基本方)으로는 교감단(交感丹), 이진탕(二陳湯), 육미삼릉환(六味三稜丸)을 가장 많이 사용하였음을 알 수 있다.