• Title/Summary/Keyword: patch test

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First Report of Summer Patch Caused by Magnaporthiopsis poae on Cool Season Grass (Magnaporthiopsis poae에 의한 한지형 잔디의 여름잎마름병 보고)

  • Han, Ju Ho;Ahn, Chang Hyun;Lee, Seung-Yeol;Back, Chang-Gi;Kang, In-Kyu;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.196-200
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    • 2016
  • Symptoms of summer patch were observed on Kentucky bluegrass (Poa pratensis L.) cv. "Midnight II" from mid-June in 2015 in Seoul, Korea. The symptoms appeared as leaf blight, root rot, and frog-eye patch, which are typical of summer patch. To identify the causal agent of these symptoms, a pathogen was isolated from diseased leaves and roots, and the cultural, morphological, and phylogenetic characteristics were analyzed. The isolate reached 50-60 mm on potato dextrose agar (PDA) after 10 days as a white-grey mycelium with septa, and became olive-green or brown from the center. Phialide-like structures were observed at the ends of hyphae, and conidia were rarely observed. A phylogenetic analysis was conducted based on large subunit (LSU) and RNA polymerase II large subunit (RPB1) sequences. According to this analysis, the isolated pathogen was confirmed to be Magnaporthiopsis poae. In a pathogenicity test, summer patch symptoms were observed at 20 days after inoculation using the same grass cultivar. This is the first report of summer patch disease caused by M. poae on cool season grass in Korea.

Cytotoxicity Evaluation of Cosmetic Materials to Mouse Fibroblast : by Tetrazolium salt, MTT Colorimetric Assay (Tetrazolium salt, MTT Colorimetric Assay를 이용한 Mouse Fibroblast에 대한 화장품원료 물질의 세포독성 평가)

  • Jo, Jae- Hoon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.15 no.1
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    • pp.37-50
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    • 1989
  • The in Vitro chemosensitivity of fibroblast cell strains was determined using a semiautomated tetrazolium-based colorimetric assay(MTT assay) to 16 cosmetic materials. This assay is useful method to evaluate toxic effects of the chemicals. From assay results, we determined that the preservatives are more toxic than moisteurizers. The chemicals in the same group have a different toxicity. That is, in preservatives, Germall -115 is more toxic than Danisol -M, -p, and in surfactant sodium laurel sulfate than Myrj 52, and in moisteurizers, 1, 3-butylene glycol is more safe than the others. When the results from this assay for preservatives were compared with patch test results, good correlation was observed. Forthemore, this assay method can be used together with Patch test for the evaluation of the chemical toxicity, particularly in cosmetic field.

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OBSERVATION ON PATCH TEST USING A FEW DENTAL MEDICAMENTS BASED ON NURSING ASPECT (치과용 약제 접촉이 인체피부조직에 미치는 영향에 관한 간호학적 고찰)

  • Han, Song-Ja
    • The Journal of the Korean dental association
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    • v.16 no.12 s.115
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    • pp.941-944
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    • 1978
  • The author studied skin reactions and their incidences among 100 dental students and dental nurses to 2% Lidocaine HC1, 3% H₂O₂, Euginol, 5% Sodium Hypochlorite, Dycal and Adhesive plaster by means of Patch test. Following are the results of the study. 1. Eighty six cases out of 100 revealed slight response to 1 or 2 allergens. 2. 2% Lidocaine HC1 showed 1 case, 3% H₂O₂cases, Euginol 2 cases, 5% Sodium Hypochlorite 74 cases, Dycal 1 case Adhesive plaster 8 cases. 3. 5% Sodium Hypochlorite was the most influential to skin. 32 cases showed grade 1+, 20 cases showed grade 2++, 16 cases showed grade 3+++, 6 cases showed grade 4++++,

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Development of Laser Speckle Flowgraphy System for Monitoring Blood Flow in Skin Tissue (레이저 산란 현상을 이용한 피부혈류 화상화 시스템의 개발)

  • Lee, M.C.;Fujii, H.
    • Journal of Biomedical Engineering Research
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    • v.28 no.3
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    • pp.392-396
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    • 2007
  • In this paper, we develop a new system to visualize the blood flow map in skin tissue, using the technique of Laser Speckle Flowgraphy (LSFG). The measuring unit consists of the laser diode, imaging system, line sensor, scanning mirror, and one-board microcomputer. The speckle signal is analyzed and sent to a PC, where the blood flow in a tissue area of $14mm{\times}26 mm$ is evaluated and displayed in a 2-D color map. It is demonstrated that the new LSFG instrument is useful to evaluate the degree of allergic reaction in patch test.

Calibration of Hydrographic Survey Multibeam System Using Terrestrial Laser Scanning and TS Surveying (지상 레이저 스캐닝과 TS 측량을 이용한 멀티빔 시스템의 검·보정)

  • Kim, Jin Soo
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.31 no.3
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    • pp.199-207
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    • 2013
  • In hydrographic survey, data surveyed with multibeam system includes various errors due to multiple factors. These are corrected by a calibration called patch test, and if existing method is used, the test needs to be conducted for about 8 times for precise system calibration. For more prompt and precise multibeam system calibration, the exact offset of a ship was determined using terrestrial laser scanning and TS surveying, which was used as the initial input for the patch test. In the result, the error of closure was 0.001 m or less for TS surveying and backsight error was 0.005 m or less for scanning. All the surveying data based on the same local coordinate was converted into vessel reference coordinate during which R-square for all rotation angles was 0.99 or higher and standard deviation was 0.008 m or less. Finally, in a patch test using calculated offset of sensors and motion sensor offset, the offset of MBES transducer satisfied manual on hydrography only with 1-time calibration. With these results, it is thought that terrestrial laser scanning and TS surveying can fully be utilized for multibeam system calibration.

Analysis of Characteristics of Peripheral Arterial Ischemia in Premature Babies and Effects of Nitroglycerin Patch Application

  • Kim, Jeongeun;Lee, Jin Won;Kim, Dong Yeon
    • Child Health Nursing Research
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    • v.26 no.4
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    • pp.434-444
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    • 2020
  • Purpose: The aim of this retrospective study was to analyze the characteristics of peripheral arterial ischemia and tissue necrosis in premature babies, as well as the effects of nitroglycerin. Methods: In total, 513 newborns were enrolled who were admitted to the neonatal intensive care unit with a gestational age of 34 weeks or younger. Data were collected on general personal and clinical information, peripheral arterial ischemia, and nitroglycerin patch application in the premature infants. The collected data were analyzed using the χ2 test, t-test, Mann-Whitney U test, logistic regression. Results: Thirty-six (7.0%) infants had peripheral arterial ischemia, while 477 (93.0%) infants did not. Lower gestational age (χ2=35.97, p<.001), lower birth weight (χ2=29.40, p<.001), lower blood pressure (χ2=23.10, p<.001), and insertion of an umbilical artery catheter (p<.001) were significantly associated with the occurrence of peripheral arterial ischemia. Among the preterm infants in whom nitroglycerin patches were applied, 30 (83.3%) premature infants without necrosis improved without complications, 4 (11.1%) showed hypotension, and 2 (5.6%) showed skin damage. Conclusion: Based on a review of our experiences with nitroglycerin patches, we recommend closely observing skin color and using nitroglycerin patches on the skin to help improve flow in premature infants with peripheral arterial ischemia.

Inhibition of in Vitro Growth of Three Soil-borne Turfgrass Diseases by Antagonistic Bacteria from Composted Liquid Manure (가축분뇨액비의 길항미생물에 의한 토양전염성 병원균의 생육억제 효과)

  • Ryu, Ju Hyun;Shim, Gyu Yul;Kim, Ki Sun
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.879-886
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    • 2014
  • This study was conducted to test in vitro the antagonistic effect of composted liquid manure (CLM) against soil-borne turfgrass pathogenic fungi, Rhizoctonia solani AG-2-2 (IIIB) (brown patch), R. solani AG-2-2 (IV) (large patch), and Sclerotinia homoeocarpa (dollar spot) for environmentally friendly turfgrass management. CLMs were collected from 9 livestock excretion treatment facilities around the country including Gunwi (GW), Hapcheon (HC), Hoengseong (HS), Icheon (IC), Iksan (IS), Muan (MA), Nonsan (NS), and Yeoju (YJ). CLMs of IC, GW, and IS showed s ignificant (p < 0.05) mycelium growth inhibition that was 17.8%, 20.4%, and 48.0% against R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. A t otal of 110 bacterial isolates were obtained from the CLMs that showed antagonistic effects. Among them, 5, 4, and 10 microbe isolates showed promising antifungal activity against mycelium growth of R. solani AG-2-2 (IIIB), R. solani AG-2-2 (IV), and S. homoeocarpa, respectively. The bacterial isolates ICIIIB60, GWIV70, and ISSH20 effectively inhibited the mycelial growth of three soil-borne turfgrass pathogens. Selected bacterial isolates were identified as Alcaligenes sp., Bacillus licheniformis Ab2, and B. subtilis C7-3 through 16s rDNA gene sequence analysis. Among 5 fungicides, the most compatible fungicide with ICIIIB60, GWIV70, and ISSH20 was tebuconazol, toclofos-methyl and toclofos-methyl, respectively. These findings suggested that CLMs could be effectively used not only as organic liquid fertilizer sources but also as biological control agents for soil-borne turfgrass diseases such as brown patch, large patch, and dollar spot.

Suppression of Rhizoctonia spp. by Antagonistic Microorganisms and Their Compatibility with Fungicides (길항미생물에 의한 Rhizoctonia spp.의 억제 및 길항미생물의 농약 혼용시 생존율)

  • 이상재;심경구;김영권;허근영
    • Asian Journal of Turfgrass Science
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    • v.12 no.1
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    • pp.23-30
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    • 1998
  • 174 isolates of soil microorganisms were isolated from E-golf club from Apr.1997 through Oct. 1997. And 27 strains of them were selected through the inhihition test of mycelial growth. In the same period, soil-borne diesease pathogens, "Rhizoctonia", causing Large patch, Brown patch, Spring dead spot, and Yellow patch were isolated from the diseased areas in E-golf and S-golf club. The antagonistic activity of the strains against the pathogens was tested to select the excel-lent antagonists. In contact with the fungicides, the survivability of the antagonists was tested to assess the compatibility of the antagonists with the pesticides. The results were as follows: 1.Suppression of Rhizoctonia by Antagonists. Antagonistic activity of 27 strains against the pathogens was: tested in vitro. In the result, 3 isolates(B-7, B-15, B-41) of bacteria and 2 isolates(F-5, F-47) of fungi were superior to the rest. 2.Compatibility of the antagonists: with the fungicides: With 13 kinds of pesticides widely using Golf Club, Compatibility of 5 antagonists: were finally tested to select the strains: that mostly survived in contact with pesticides. In the results:, two of five strains: were selected : one strain was bacteria B-15, the other strain was fungi F-47. 24h after the mixing with pesticides:, these two strains were shown to survive at 90% level and these were identified as Bacillus and Trichoderma, respectively. And the most compatible pesticides: with the antagonists were shown to Polytoxin-D thirarn(s:urvivability 99.4%) and Validamycin-A (survivability 98.6%). Keywords:Antagonist, Large Patch, Trichoderma, Compatibility, Fungicide.Fungicide.

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Surface Damage and Bleaching Effect according to the Application Type of Home Tooth Bleaching Applicants

  • Tak, Na-Yeoun;Lim, Do-Seon;Lim, Hee-Jung;Jung, Im-Hee
    • Journal of dental hygiene science
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    • v.20 no.4
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    • pp.252-260
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    • 2020
  • Background: In this study, the bleaching effect and surface damage of two types of over-the-counter home tooth bleaching agents were explored using an in vitro study of bleaching agents applied to bovine teeth specimens for 14 days. Methods: Domestic over-the-counter home tooth bleaching agents of gel and patch form that shared common active ingredients and manufacturers were selected and tested. The experiment specimens were made using composite resin with bovine tooth samples and then measured the initial microhardness. Specimens were then divided into a Gel group and a Patch group and underwent bleaching treatment once a day for two weeks for 30 to 60 minutes (recommended) or 7 hours. All specimens were coffee-stained prior to bleaching. The bleaching effect was measured using a spectrophotometer and surface damage was measured using a microhardness meter. Results: The difference in color following the bleaching procedure was positive in both the Gel and Patch group, although there were no statistically significant differences in bleaching effect between groups. There was no significant difference in bleaching effect based on duration. The microhardness test revealed that both the Gel group and the Patch group had surface damage after bleaching. The greatest surface damage was found in the Patch group that had undergone a 7-hour bleaching treatment, although the differences were not statistically significant. Conclusion: The bleaching effect of the home tooth bleaching agent was visible to the naked eye. However, longer applications than recommended did not result in greater bleaching, unlike consumers' expectations, and instead increased the chance of enamel damage. As such, there is a need for consumers to be alert and adhere to recommendations provided by each company.

Flip Error Resistant Stitching in Sensor Network Localization (센서네트워크의 위치추정에 있어 플립오류에 강건한 스티칭 기법)

  • Kwon, Oh-Heum;Park, Sang-Joon;Song, Ha-Joo
    • Journal of KIISE:Information Networking
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    • v.36 no.1
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    • pp.24-33
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    • 2009
  • In patch-and-stitch localization algorithms, a flip error refers to the kind of error in which a patch is stitched to the map as being wrongly reflected. In this paper, we present an anchor-free localization algorithm which tries to detect and prevent flip errors. The flip error prevention is achieved by two filtering mechanisms: the flip-ambiguity test and the flip-conflict detection. We evaluate the performances of proposed techniques though simulations and show that they achieve significant performance improvements.