• Title/Summary/Keyword: pat

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Advanced Array Test Sensor

  • Lee, Jong-Ho;Pye, Tom;Maxton, Pat
    • 한국정보디스플레이학회:학술대회논문집
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    • 2007.08a
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    • pp.283-285
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    • 2007
  • This paper will discuss the latest results from an advanced array test system, using newly developed sensor technology. A comparison of detection results between old and new generation products will be shown along with a discussion of the advantages of the new, higher signal to noise ratio detector.

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Transgenic Plants of Easter Lily (Lilium longiflorum) with Phosphinothricin Resistance

  • Ahn, Byung Joon;Joung, Young Hee;Kamo, Kathryn K.
    • Journal of Plant Biotechnology
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    • v.6 no.1
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    • pp.9-13
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    • 2004
  • Transient uidA expression was used to optimize parameters required for biolistic transformation of suspension cells of Easter lily, Lilium longiflourm. Maximum uidA expression occurred following bombardment with gold particles as compared to tungsten. A 3hr pre-treatment of suspension cells with 0.125M osmoticum resulted in a 1.5X increase in uidA expression. A helium pressure of 1550 psi combined with a particle travelling distance of 6cm resulted in maximum uidA expression as compared to either 1100, 1200, or 1800 psi. Transient transformation resulted in up to 493 uidA expressing cells/Petri plate. For stable transformation suspension cells of Lilium longiflorum, were co-bombarded with plasmid DNA containing cucumber mosaic virus (CMV) replicase under the rice actin (Act1) promoter and either the bar or PAT genes under the cauliflower mosaic virus (CaMV 355) promoter. Ten regenerated plants contained the transgene as analyzed by PCR, and two of the ten plants were confirmed to contain the transgene by Southern hybridization. The two transgenic plants were independent transformants, one containing the bar gene and the other both the CMV replicase and bar genes. Plants were sprayed at the rosette stage and found to be resistant to 1000 mg/L of phosphinothricin (Trade name-Ignite) indicating expression of the bar gene throughout the leaves when bar was under control of the CaMV 35S promoter.

Anti-stress effects of Sihosogansan in the passive avoidance test and the forced swimming test (시호소간산(柴胡疏肝散)이 스트레스로 인한 기억저하와 우울행동에 미치는 영향)

  • Jung, Min-Ho;Lee, Tae-Hee
    • The Korea Journal of Herbology
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    • v.22 no.2
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    • pp.127-135
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    • 2007
  • Objective : Investigation of the anti-stress effects of Sihosogansan Methods : Passive avoidance test(PAT) was performed after applying immobilization stress in water to rats. Also, forced swimming test(FST) was performed to another rats and after FST, the degree of Tyrosine Hydroxylase(TH) expression was measured with immunohistochemical method in the regions of locus coeruleus(LC) and ventral tegmental area (VTA). Results : In the PAT after immobilization stress in water, response latency was significantly increased in the Sihosogansan(400mg/kg) group in comparison with the control group. In the FST, immobility was significantly decreased in the Sihosogansan groups (100mg/kg, 400mg/kg), comparing with the control group. Stress-induced TH increases were suppressed in the Sihosogansan groups (100mg/kg, 400mg/kg) at the LC and the VTA region respectively. Conclusion : Sihosogansan can improve memory ability of rats, reduce behavior of depression in rats, decrease TH-immunoreactive cells at the LC and VTA region in rat, and it may be concluded that Sihosogansan has significant effect in reducing stress.

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Antidepressant Effects of Gammakdaejo-Tang on Repeated Immobilization Stress in the Ovariectomized Female Rats

  • Park, Hyun-Jung;Shim, Hyun-Soo;Lee, Hye-Jung;Yun, Young-Ju;Shim, In-Sop
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.5
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    • pp.876-880
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    • 2011
  • Gammakdaejo-Tang (GMT) is a traditional oriental medicinal formula, a mixture of 3 crude drugs, and it has been clinically used for treating mild depressive disorders. The purpose of the study was to examine the effect of Gammakdaejo-Tang (GMT) on repeated stress-induced alterations of learning and memory on a passive avoidance test (PAT) test and also the anxiety-related behavior on the elevated pulse maze (EPM) in ovariectomized female rats. We assessed the changes in the reactivity of the cholinergic system by measuring the immunoreactive neurons of choline acetyltransferase (ChAT) in the hippocampus after behavioral testing. The rats were exposed to the immobilization (IMO) stress for 14 days (2hours/day), and Gammakdaejo-Tang (400 mg/kg, p.o.) was administered 30 min before IMO stress. Treatments with GMT caused significant reversals of the stress-induced deficits in learning and memory on a working memory test, and it also produced an anxiolytic-like effect on the EPM, and increased the ChAT reactivities (p<0.001, respectively). These results suggest that Gammakdaejo-Tang might prove to be an effective antidepressant agent.

The Effect of the Dietary Carbohydrate on Lipid and Ca Metabolism, and the Immune Function in Aged Rats ($\cdot$저 탄수화물 식이로 사육된 흰 쥐의 노화과정중 나타나는 지방과 Ca 대사 및 면역능력에 미치는 영향연구)

  • 윤군애
    • Journal of Nutrition and Health
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    • v.20 no.2
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    • pp.135-144
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    • 1987
  • This study was carried out to investigate the effect of dietray carbohydrate level on the lipid and Ca metabolism, and on immune response in the process of aging. Sprag\ulcornerue - Dawley male rats of 12 months of age were fed either high carbohydrate or low carbohydrate diet for 9 months. With increasing age from 12 months to 21 months, there was no significant increase in body weight, however, the epididymal fat pat weight and total serum lipid tend to become higher, and the inorganic/organic matterratio in bone tends to become lower. This suggests that body fat accumulation increase and bone mass decrease with age. Rats fed low carbohydrate diet showed the higher epididymal fat pat weight, and the lower Ca content in femur and scapular compared to high carbohydrate diet fed rats. The immune response was also lower in low carbohydrate diet group judged by the lower thymus and spleen index and by the lower response to PHA and ConA stimulation. The results of this study suggests that the dietary level of carbohydrate and fat may play an important role in the process of aging. It could be recommended to practice high carbohydrate -low fat diet for the elderly to reduce the risk of degenerative diseases.

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Design of Visual Surveillance System based on Wireless High Definition Image Transmission Technology (무선 고해상도 영상 전송 기술에 기반한 영상 감시 시스템의 설계)

  • Kim, Won
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.12 no.5
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    • pp.25-30
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    • 2012
  • It is important to detect dangerous objects which are intentionally abandoned in public places. Nowadays visual surveillance system is required to enhance the performance in two ways : high resolution and wireless linking ability. In this study the design of visual surveillance system is newly proposed to detect abandoned objects for social security purpose based on wireless high resolution image transmission technology. Also, to enhance PED, PAT performance, the tracking algorithm is included in the previous visual surveillance software scheme. By implementing proposed design scheme on the real wireless high resolution image transmission system, the effectiveness of the overall system is shown with the transmission performance of 4.0 Gbps speed.

Design on Smart Security Disk System with Wireless Interface of High Definition Image (고해상도 영상의 무선 인터페이스를 갖는 스마트 보안 디스크 시스템의 설계)

  • Kim, Won
    • Journal of Digital Convergence
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    • v.11 no.9
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    • pp.195-200
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    • 2013
  • In visual surveillance system abandoned objects in public places are the deliberately left things, which should be automatically detected by intelligent systems in the environment where the number of cameras is increasing. This research deals with the design scheme of a smart security disk system which can detect these abandoned objects automatically and save the relevant image information with the wireless interface of high definition images. By implementing the proposed system in this research it is confirmed that the transmission performance shows 60 frames per second without compression of high definition images and the capability of the disk system shows the relevant images can be saved in a RAID configuration. Also, the proposed visual surveillance software shows a good detection rate of 80% in PAT performance.

Protoplast Isolation and Regeneration of Fertile Plants from Arabidopsis Trp Mutant, trp1-100

  • Lim, Seon-hee;Kim, Young-soon;Lee, Eui-seung;Rose, Alan;Last, Robert;Cheong, Hyeon-sook
    • Animal cells and systems
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    • v.2 no.2
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    • pp.239-242
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    • 1998
  • Arabidopsis trp1 mutant plants, deficient in phosphoribosyI anthranilate transferase (PAT) activity, accumulate anthranilate compounds, which render them blue fluorescence. The visible phenotype of trp1 makes the PAT gene an excellent reporter gene in the mutant. In order to develop a system for the homologous recombination using the phenotypic characteristic of trp1-100, we established optimum conditions for the isolation and regenera tion of protoplast from auxin-conditioned, trp1-100 root cultures. Trvptophan had to be supplemented in the germination medium for the efficient cell division and subsequent plant regeneration. When 10 uM tryptophan was added to the germination medium, we obtained the highest yield of protoplasts ($3{\times}10^6 cells/g$) and the best viability (92%). Thirty percent of root protoplast derived from meristematic cells underwent cell division within 5 days in callus-induction medium. Regenerated rosette leaves (2-3 mm) were transferred to rooting medium and finally acclimated to the soil for flowering.

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Differences in Yields, Antioxidant Compounds, and Antioxidant Activity of Ethanolic Extracts among 11 Adzuki Bean Cultivars (Vigna angularis L.) Cultivated on a Somewhat Poorly Drained Paddy Field (논 재배 팥 품종별 수량구성요소 및 에탄올 추출물의 항산화 성분 비교)

  • Chun, Hyen Chung;Jung, Ki Yuol;Choi, Young Dae;Lee, Sanghun;Song, Seok bo;Ko, Jee Yeon;Choi, Ji Myung;Jang, Yun Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.3
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    • pp.203-213
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    • 2017
  • This study investigated the changes in yields, antioxidant compounds, and antioxidant activities among 11 adzuki bean cultivars cultivated in a paddy field with somewhat poorly drained soil. The adzuki bean cultivars were cultivated in the paddy field from 2015 to 2016 in Milyang, Gyeongsangnam, Korea. Average soil moisture content was 16.5% in 2015 and 31.0% in 2016 at the experimental site during adzuki bean cultivation. As the soil moisture content increased, most of the adzuki bean cultivars showed deceases in stem height, first setting pod node, number of pods, 100 seed weight, and yield. Chungju-pat produced the greatest yields among the 11 cultivars in 2015 and 2016, whereas Hongeon had the smallest yields. Adzuki bean yields after paddy field cultivation was lower for all cultivars than for adzuki bean yields from the same cultivars after upland field cultivation. Chungju-pat and Chilbo-pat showed the smallest decreases in yields after paddy field cultivation, whereas Hongeon and Kumsil showed the greatest yield reductions. There were no significant differences in proximate composition. Some mineral components ($P_2O_5$, Ca, and Mg) were statistically different across cultivars. Chungju-pat had the highest Ca and Mg contents, but antioxidant components (polyphenol and flavonoids) and antioxidant activities (ABTS and DPPH) were highest in Saegil and lowest in Jungbu-pat. PCA and clustering analyses, based on the growth, yield, and antioxidant component measurements, performed to identify which variables contributed the most to separating adzuki bean cultivars or to grouping cultivars with similar characteristics. These analyses showed that the antioxidant components and antioxidant activities had the most influence on grouping cultivars together. Among the 11 cultivars, Saegil was statistically different from the other cultivars, but the other 10 cultivars were not significantly different under paddy field cultivation. Soil moisture content affected adzuki bean yield and antioxidant component contents. An increase in soil moisture led to a decrease in yield, but an increase in antioxidant components. These results provide information that will improve the selection of an appropriate adzuki bean cultivar for use in paddy fields.