• Korean Journal of Clinical Laboratory Science
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• v.47 no.4
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• pp.292-297
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• 2015

Research of thyroid cancer, liver cancer, and lung cancer has been reported in Korea. However microRNA research of multiple myeloma has never been reported. Hence we intended to confirm whether microRNA can be utilized as a diagnostic marker to patients of multiple myeloma. We also intended to evaluate whether microRNA can be detected in paraffin-embedded tissue (FFPE). This research was conducted targeting 8 samples from patients of multiple myeloma who do not have any other diseases, and 2 control samples. From January 2010 to July 2012, we selected miR-15a, miR-16, miR-21, miR-181a and miR-221 as microRNA target genes. It was decided that for a sample to be significant, the results should show values more than 1.5 or less than -1.5. Our findings of fold change were highly significant in miR-15a with a value of 37.5% (3/8). From these studies, we learned that miR-15a is useful with westerners. miR-221, on the other hand, shows conflicts with westerners, so more research will be needed in this area. In addition, it was confirmed that microRNA can be detected in paraffin embedded tissue (FFPE).

• Current Optics and Photonics
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• v.4 no.1
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• pp.31-43
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• 2020

The feasibility of the application of terahertz electromagnetic waves in the diagnosis of prostate cancer was examined. Four samples of incomplete cancerous prostatic paraffin-embedded tissues were examined using terahertz spectral imaging (TPI) system and the results obtained by comparing the absorption coefficient and refractive index of prostate tumor, normal prostate tissue and smooth muscle from one of the paraffin tissue masses examined were reported. Three hundred and sixty cases of absorption coefficients from one of the paraffin tissues examined were used as raw data to classify these three tissues using the Principal Component Analysis (PCA) and Least Squares Support Vector Machine (LS-SVM). An excellent classification with an accuracy of 92.22% in the prediction set was achieved. Using the distribution information of THz reflection signal intensity from sample surface and absorption coefficient of the sample, an attempt was made to use the TPI system to identify the boundaries of the different tissues involved (prostate tumors, normal and smooth muscles). The location of three identified regions in the terahertz images (frequency domain slice absorption coefficient imaging, 1.2 THz) were compared with those obtained from the histopathologic examination. The tissue tumor region had a distinctively visible color and could well be distinguished from other tissue regions in terahertz images. Results indicate that a THz spectroscopy imaging system can be efficiently used in conjunction with the proposed advanced computer-based mathematical analysis method to identify tumor regions in the paraffin tissue mass of prostate cancer.

• Journal of Genetic Medicine
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• v.2 no.2
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• pp.53-57
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• 1998

Spinal muscular atrophy (SMA) type I is a common severe autosomal recessive inherited neuromuscular disorder that has been mapped to chromosome 5q11.2-13.3. The survival motor neuron (SMN) gene, a candidate gene, is known to be deleted in 96% of patients with SMA type I. Presently, PCR and single strand conformation polymorphism (PCR-SSCP) analyses have been made possible for application to both archival slides and paraffin-embedded tissues. Archival materials represent valuable DNA resources for genetic diagnosis. We applied these methods for the identification of SMN gene of SMA type I in archival specimens for the prenatal diagnosis. In this study, we performed the prenatal diagnosis with chorionic villus sampling (CVS) cells on two women who had experienced neonatal death of SMA type I. DNA extraction was done from archival slide and tissue materials and PEP-PCR was performed using CVS cells. In order to identify common deletion region of SMN and neuronal apoptosis-inhibitory protein (NAIP) genes, cold PCR-SSCP and PCR-restriction site assay were carried out. Case 1 had deletions of the exons 7 and 8, and case 2 had exon 7 only on the telomeric SMN gene. Both cases were found to be normal on NAIP gene. These results were the same for both CVS and archival biopsied specimens. In both cases, the fetuses were, therefore, predicted to be at very high risk of being affected and the pregnancy were terminated. These data clearly demonstrate that archival slide and paraffin-embedded tissues can be a valuable source of DNA when the prenatal genetic diagnosis is needed in case any source for genetic analysis is not readily available due to previous death of the fetus or neonate.

• Journal of Radiation Protection and Research
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• v.20 no.4
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• pp.237-243
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• 1995

An estimate is made on the potential generation rate of H: from radiolysis of the Paraffin-wax encapsulant Proposed for the solidified liquid concentrate wasteform. The results show that the radiolytic Production of $H_2$ from paraffin-wax-encapsulated waste is dominated by the radiation energy released from $^{60}Co$. The radiolytic production of $H_2$ will proceed at an initial rate equivalent to aproximately $4.4{\times}10^2cm^3yr^1$ in 200 litre drums that are partly filled with 120 litres of encapsulated waste. The gas production rate will fall to a value of $7.2cm^3yr^1$ after 100 years. The lower flammable limit for $H_2$ in air will be reached in about 25 years and the lower explosive limit for $H_2$ in air would not be reached in 1000years. The timescale in which these safety-related limits are reached is strongly dependent on the level of filling of each waste drum. A reduction of the air space inside each drum would reduce the time required to reach the lower flammable limit.

• Clean Technology
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• v.18 no.2
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• pp.155-161
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• 2012

The purpose of this study is to remove paraffin wax binder effectively from powder injection molded part using supercritical fluids in powder injection molding process. For a thin powder injection molded part about 1-2 mm thickness, paraffin wax binder can be removed rapidly without any defect by traditional supercritical extraction process which has fixed high temperature and pressure condition. But, for a thick powder injection molded part, there are limitations in removing paraffin wax binder by the fixed high process condition because crack occurs at the beginning step. Therefore, here we studied variable condition debinding process that starts with mild process condition at the beginning step and then increase the process conditions simultaneously at each step. To find out the initial process condition that has the highest extraction yield without any defect for each sample thickness, we investigated various supercritical debinding conditions using 1-4 mm thickness ceramic injection molded sample. By using the variable condition debinding process that starts with the initial process condition at the first step and then increasing process conditions simultaneously at each step (temperature from 333.15 to 343.15 K, pressure from 12 to 27 MPa, and $CO_2$ flow rate from 1.5 to 10 L/min), over 95% of paraffin wax binder was removed from the 4 mm thick (10 mm diameter) ceramic injection molded disk samples within 5 hours.

• Korean Journal of Plant Resources
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• v.30 no.1
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• pp.29-37
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• 2017

The effect of packaging materials on the quality and physiological activity of Angelica acutiloba leaves during storage at $4^{\circ}C$ for 20 days and after being transferred to room temperature for 2 days was studied. The experiment was conducted using three packaging materials: the corrugated box (control); corrugated box with $20{\mu}m$ HDPE perforated film liner; and corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner. Changes in weight, color, aroma, overall visual quality, antioxidant activity, and total flavonoids were investigated. Based on the result, the treatments using corrugated box with $20{\mu}m$ HDPE perforated film liner and the corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner, were effective in minimizing weight loss, and maintaining external color and overall quality as compared to corrugated box only (control). The corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner was more effective in maintaining antioxidant activity and total flavonoids than other treatments. Prolonging the freshness using corrugated box with paraffin wax coated paper $+20{\mu}m$ HDPE perforated film liner could increase the marketability of Angelica acutiloba leaves in the domestic market.

• Journal of the Korea Institute of Building Construction
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• v.11 no.5
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• pp.435-441
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• 2011

The life cycle assessment on greenhouse gas emission of reinforced concrete buildings shows that more than 70 percent of greenhouse gas that is discharged by a building is discharged in the building maintenance stage, including cooling and heating. To reduce the greenhouse gas emission, maintenance planning to minimize the energy consumption is necessary in the design stage. In this paper, two heat storage rooms are tested to save the air cooling energy of the buildings. The specimens are essentially identical, except that chamber A contained paraffin sheets as the finishing material, while the other, chamber B, served as a control. The test results show that chamber A with the paraffin sheets exhibited less temperature change than chamber B without the sheets when temperature was increased outside of the specimens. The heating energy was probably consumed in the phase change of the paraffin sheets, which can be useful for reducing energy consumption related to air cooling during the summer.

• Korean Journal of Food Science and Technology
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• v.6 no.4
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• pp.231-240
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• 1974

The growth of a mixed yeast culture consisting of Canda tropicalis var. KIST 76 and Tricosporon cutaneum KIST 76-H was compared with that of pure cultures under pilot plant conditions. The mixed culture was judged stable based on the nearly constant ratio of the two organisms at the completion of fermentation. We obtained higher cell yields, protein content and productivity in the mixed culture on n-paraffin than the pure culture of C. tropicalis var. KIST 76. T. cutaneum KIST 76-H did not grow on n-paraffin medium. With the batch cultivation of mixed organisms on n-paraffin, the specific growth rates during the exponential growth phase were 0.24-0.33 $hr^{-1};$ cell yields were 96-106% and productivities were 2.9-3.6g/l. hr. The cells obtained contained 55-58% crude protein and 5.5-6.3% lipid. The critical value of dissolved oxygen concentration Ccrit. and saturation constant, km, are approximately 1.5 ppm and 0.228 ppm respectively. Also we established the optimal conditions for the mixed culture in batch fermentation.

• Clean Technology
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• v.29 no.4
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• pp.262-271
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• 2023

Globally, the amount of waste plastics has been occurring to environmental problems. As a result, it is necessary to research methods that utilize waste plastic pyrolysis oil (WPPO) produced by pyrolysis. One such method being studied is utilizing WPPO as a naphtha feedstock. In this study, five types of WPPO were analyzed to determine whether they can be used as raw materials for naphtha. Because of their wide boiling point range, the WPPOs were fractionated into light and heavy fractions through vacuum distillation, and the separation and purification techniques were analyzed using GC-VUV to determine the content of paraffin, olefin, and other compounds. All WPPOs showed high olefin content regardless of the source and fraction. Aromatic and paraffin content varied depending on the source, and oxygen and other compounds also varied significantly by source and fraction. In addition, the light fraction showed a carbon distribution similar to that of naphtha, whereas the heavy fraction showed a carbon distribution of C₁₁ ~ C₁₄. In conclusion, additional processes and raw material selection are required to utilize waste plastic pyrolysis oil as a raw material for naphtha.

• Progress in Superconductivity and Cryogenics
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• v.26 no.1
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• pp.20-24
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• 2024

Over the past four years, as the COVID-19 pandemic has struck the world, cold chain of COVID-19 vaccination has become a hot topic. In order to overcome the pandemic situation, it is necessary to establish a cold chain that maintains a low-temperature environment below approximately 203K (-70℃), which is the appropriate storage temperature for vaccines, from vaccine suppliers to local hospitals. Usually, cryocoolers are used to maintain low temperatures, but it is difficult for small-scale local distribution to have cryocooler due to budget and power supply issues. Accordingly, in this paper, a cryogenic TSU (Thermal storage unit) system for vaccination cold chain is designed that can maintain low temperatures below -70℃C for a long time without using a cryocooler. The performance of the TSU system according to the energy storage material for using as TSU is experimentally evaluated. In the experiments, four types of cold storage materials were used: 20% DMSO aqueous solution, 30% DMSO aqueous solution, paraffin wax, and tofu. Prior to the experiment, the specific heat of the cold storage materials at low temperature were measured. Through this, the thermal diffusivity of the materials was calculated, and paraffin wax had the lowest value. As a result of the TSU system's low-temperature maintenance test, paraffin wax showed the best low-temperature maintenance performance. And it recorded a low-temperature maintenance time that was about 24% longer than other materials. As a result of analyzing the temperature trend by location within the TSU system, it was observed that heat intrusion from the outside was not well transmitted to the low temperature area due to the low thermal conductivity of paraffin wax. Therefore, in the TSU system for vaccine storage, it was experimentally verified that the lower the thermal diffusivity of the cold storage material, the better low temperature maintenance performance.