• Animal cells and systems
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• 제4권2호
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• pp.187-193
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• 2000

Regional distribution and relative frequency of endocrine cells in the pancreas of the red-eared slider, Trachemys scripta elegans, were investigated by immunohistochemical methods. Chromogranin (Cg) A-, serotonin-, insulin-, glucagon-, somatostatin-, bovine pancreatic polypeptide (BPP)- and human pancreatic polypeptede (HPP)-immunoreactive cells were identified in this study. Most of immunoreactive cells in the exocrine and endocrine pancreas (Langerhans islet) were generally spherical or spindle-shaped (open-typed cell), while occasionally cells round in shape (close-typed cell) were found in the basal portion or interepithelial regions of the pancreatic duct. These immunoreactive cells were located in the exocrine, endocrine pancreas and/or basal or interepithelial portion of the pancreatic duct. Serotonin-immunoreactive cells were found in the basal portion of epithelia of the pancreatic duct at a low frequency and interacinar region of the exocrine at a moderate frequency. Insulin-immunoreactive cells were found in the central portion of the endocrine pancreas, interacinar regions of the exocrine pancreas and basal portion of the epithelia of the pancreatic duct at high, moderate and low frequencies, respectively. Glucagon-immunoreactive cells were detected in the periphery of the endocrine pancreas, interacinar region of the exocrine pancreas and basal portion of the epithelia or interepithelia of the pancreatic duct at high, moderate and moderate frequencies, respectively. Somatostatin-immunoreactive cells were dispersed in the whole area of the endocrine pancreas, interacinar regions of exocrine pancreas and basal portion of the epithelia or interepithelia of the pancreatic duct at a moderate frequency. BPP- and HPP-immunoreactive cells were detected in the iinteracinar region of the exocrine pancreas at moderate and hige frequencies, respectively. However, no Cg A- and motilin-immunoreactive cells were detected in this study.

• 대한수의학회지
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• 제33권3호
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• pp.369-379
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• 1993

북극여우의 위장췌 내분비세포의 부위별 분포, 출현빈도 및 세포의 종류를 밝히고자 면역조직화학적으로 관찰하였던 바 위장관에서는 6종, 췌장에서는 4종의 면역반응세포가 동정되었다. 5-HT 및 somatostatin면역반응세포는 전 위장관에 다수 분포하였으나 이중 somatostatin 면역반응세포는 장관에서 소수로 관찰되었다. Gas/CCK 면역반응세포는 주로 유문부와 십이지장에 국한하여 다수 분포하였다. Glucagon 면역반응세포를 분문부와 위저부에서 다수 그리고 장관에서 소수 관찰되었다. BPP 면역반응세포는 분문부와 위저부에 중등도, 대장에 소수 분포하였다. 다수의 porcine CG 면역반응세포가 분문부와 위저부에서만 동정되었다. 한편 췌장에서는 somatostatin, glucagon, BPP 및 insulin 등 4종의 면역반응세포가 췌도 및 외분비부에서 관찰되었다. 이상의 결과는 위췌장 내분비세포의 부위별 분포와 출현빈도가 동물종에 따라 매우 상이하다는 사실을 시사한다.

• 대한수의학회지
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• 제33권4호
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• pp.579-589
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• 1993

북극여우의 위장췌 내분비세포의 부위별 분포, 출현빈도 및 세포의 종류를 밝히고자 면역조직화학적으로 관찰하였던 바 위장관에서는 6종, 췌장에서는 4종의 면역반응세포가 동정되었다. 5-HT 및 somatostatin 면역반응세포는 전위방관에 다수 분포하였으나 이중 somatostatin 면역반응세포는 장관에서 소수로 관찰되었다. Gas/CCK 면역반응세포는 주로 유문부와 십이지장에 국한하여 다수 분포하였다. Glucagon 면역반응세포는 분문부와 위저부에서 다수 그리고 장관에서 소수 관찰되었다. BPP 면역반응세포는 분문부와 위저부에 중등도, 대장에 소수분포하였다. 다수의 porcine CG 면역반응세포가 분문부와 위저부에서만 동정되었다. 한편 췌장에서는 somatostatin, glucagon, BPP 및 insulin 등 4종의 면역반응세포가 췌도 및 외분비에서 관찰되었다. 이상의 결과는 위췌장 내분비세포의 부위별 분포와 출현빈도가 동물종에 따라 매우 상이하다는 사실을 시사한다.

• 대한수의학회지
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• 제36권4호
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• pp.747-755
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• 1996

한국산 두더지의 위장관 점막과 췌장에 분포하는 내분비세포들의 분포와 출현빈도 및 종류를 알아보기 위하여 면역조직화학적으로 관찰하였던 바, 5-HT 면역반응세포는 극소수내지 중등도의 빈도로 전 장관에 고루 분포하였다. Glucagon 면역반응세포는 대장에 국한하여 출현하였다. 또 bovine CG, BPP 및 somatostatin 면역반응세포들은 각각 십이지장, 공장 그리고 위저부를 제외한 전 장관에 다양한 빈도로 고루 분포하였다. Gas/CCK 면역반응세포는 유문부와 소장에서만 다수 그리고 극소수로 관찰되었으나, insulin 면역반응세포는 전 장관에서 관찰할 수 없었다. 한편 췌도의 주변부에서는 glucagon, somatostatin과 BPP 면역반응세포들이, 중심부에서는 insulin 면역반응세포가 높은 빈도로 동정되었으며 또한 외분비에서는 이들 면역반응세포들이 단독 또는 소수의 집단으로 출현하였다. 이상의 결과로써 두더지의 소화관내분비세포의 분포패턴는 고슴도치의 그것과 유사하였으나 십이지장에서 세포의 종류와 출현빈도가 낮은 것을 알 수 있었다.

• 대한수의학회지
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• 제32권3호
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• pp.321-331
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• 1992

The regional distribution and relative frequency of gastrointestinal endocrine cells were studied immunohistochemically in the gastrointestinal mucosa and pancreas of the fresh water turtle. Ten kinds of endocrine cells were identified in the gastrointestinal tract. Cholecystokinin-8-, bovine pancreatic polypetide-and glucagon-immunoreactive cells were seen throughout the gastrointestinal tract, also among them cholecystokinin-8-immunoreactive cells were most predominant in segment III. Although gastrin- and gastrin/cholecystokinin-immunoreactive cells were found from segment III to VI and X, respectively, they were numerous in segment III. Somatostatin-immunoreactive cells were observed from segment I to VII. 5-hydroxytryptamine- immunoreactive cells were detected in segment I, III, VIII, IX and X. Human pancreatic polypeptide-immunoreactive cells were demonstrated in segment V, VI, VIII, IX and X. Insulin-immunoreactive cells were found from segment III to X except for segment VIII, but rare in segment VII. Neurotensin-immunoreactive cells were found to be restricted to segment VIII, IX and X. No porcine chromogranin-, substance P- and bombesin-immunoreactive cells were detected throughout the gastrointestinal tract of the fresh water turtle. Although typical mammalian pancreatic islets encapsulated by connective tissue were not present in this species, five kinds of endocrine cells-glucagon, insulin, somatostatin, bovine pancreatic polypeptide and 5-hydroxytryptamine-were found in forming small or large groups and scattered in the exocrine gland region. However porcine chromogranin- and motilin-immunoreactive cells could not be demonstrated in the pancreas.

• 대한수의학회지
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• 제31권1호
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• pp.27-32
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• 1991

두툴상어의 췌장 내분비세포를 면역조직화학적으로 관찰하였던바, 5종의 면역반응세포들이 동정되었다. glucagon 면역반응세포는 도관의 상피간 및 췌장외분비선의 선포주위에서 한개 또는 다수의 집단으로 출현하였으며, insulin 면역반응세포는 islet의 주변부 또는 도관의 상피간에 각각 관찰되었다. somatostatin 면역반응세포는 도관의 상피간 또는 췌장외분비선의 주위에 한개 또는 집단으로 분포하였으며, 소수의 5-HT 연역반응세포는 islet의 주변부 및 췌장 외분비선의 선세포간에서 관찰되었다. BPP 면역반응세포는 극소수로 관찰되었으며, GAS/CCK외 chromogranin 면역반응세포들은 관찰되지 않았다.

• 대한수의학회지
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• 제35권1호
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• pp.45-54
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• 1995

Pancreatic endocrine cells containing glucagon, insulin, somatostatin and pancreatic polypeptide were identified in the pancreas of the Korean native goat by using immunohistochemical method. Glucagon immunoreative cells were oval or fusiform in shape and located at the periphery of the pancreatic islets. Insulin immunoreactive cells were round or oval in shape and occupied throughout the pancreatic islets except the small area of the periphery. Somatostatin immunoreative cells were oval and elliptical, and mainly located at the periphery of the pancreatic islets. Some of these cells had a cytoplasmic process. Pancreatic polypeptide immunoreactive cells were elliptical or polyhedral and located at the periphery of the pancratic islets where two or more cells formed a cell cluster. The distribution rates of glucagon, insulin, somatostatin and pancreatic polypeptide immunoreactive cells were 24.4%, 44.3%, 13.2% and 18.1% respectively.

• Animal cells and systems
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• 제3권3호
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• pp.269-273
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• 1999

The distribution and relative frequency of somatostatin-immunoreactive cells in the pancreas were studied during developmental stages (fetus, neonate, 1-month-old, 6-month-old and adult) of the Korean native goat by immunohistochemical methods. Somatostatin-immunoreactive cells were detected in the exocrine of all ages, in the endocrine portions (pancreatic islets) from the neonate, and in the pancreatic duct of the 1-month-old. The relative frequencies of these cells in the pancreatic islets increased with age. However, there were no age-related changes in the relative frequencies of somatostatin-immunoreactive cells in the exocrine and pancreatic duct. Generally, they were distributed in the interacinar spaces, the epithelium of the pancreatic duct, or dispersed in the peripheral zone of the pancreatic islets in all ages. However, clusters consisting of 3-4 cells were also found in the subepithelial connective tissues from the 1-month-old. In addition, the distributions in the endocrine portions of the adult were divided into two patterns: 1) they are dispersed in the marginal regions with moderate or low frequencies, or 2) in the inner zone with high frequencies.

• 대한수의학회지
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• 제35권1호
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• pp.55-65
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• 1995

Ultrastructures of pancreatic endocrine cells containing glucagon, insulin, somatosratin and pancreatic polypeptide were studied in the pancreas of the Korean native goat by immunohistochemical and elecron microscopy. Glucagon immunoreatctive cells were round or fusiform in shape and contained secretory granules of 200-260 nm in diameter. The secretory granules were high in electron density and had a halo between the limiting membrane and the central granule core. Insulin immunoreactive cells were round or oval in shape, and contained various sizes of secretory granules from 135 to 300 nm in diameter. The secretory granules were low or moderate electron density and had a variform halo. Somatostatin immunoreactive cells were elliptical or fusiform shape with cytoplasmic processes. They contained the secretory granules of 140-320 nm with moderate electron densities. Pancreatic polypeptide immunoreactive cells were elliptical or fusiform and contained small secretory granules with high electron densities. The secretory granules were 120-230 nm in diameter and the least in number.

• 대한수의학회지
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• 제43권3호
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• pp.339-347
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• 2003

The regional distribution and relative frequency of some endocrine cells in the pancreas of the Korean aucha perch, Coreoperca herzi Herzenstein belonging to the family Serranidae in order Perciformis, were observed using specific mammalian antisera against serotonin, insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP) by peroxidase antiperoxidase (PAP) method. The pancreas was divided into four portions (principal and secondary islets, exocrine and pancreatic duct regions). In addition, the pancreatic islet regions were further subdivided into three regions (central, mantle and peripheral regions). Spherical to spindle or occasionally round to oval immunoreactive (IR) cells were demonstrated in the pancreatic islets and exoccrine portions, but no cells were detected in the pancreatic duct portions. In the principal islets, serotonin-IR cells were not detected but most of insulin-IR cells were located in the central regions and they were also demonstrated in the mantle and peripheral regions in moderate and rare frequencies, respectively. Glucagon- and hPP-IR cells were mainly situated in the mantle regions but the cells were also demonstrated in the peripheral regions in relatively lower frequency. Somatostatin-IR cells were evenly distributed in the central and mantle regions in a few frequency and cells were also demonstrated in the peripheral regions in rare frequency. Cell clusters were consisted of hPP-IR cells that were situated in the peripheral to mantle regions. In the secondary islet portions, serotonin-IR cells were randomly distributed throughout the whole pancreatic islet regions but lower frequency was detected in the peripheral regions compared to that in central and mantle regions where cells were detected in a few frequency, respectively. Insulin-IR cells were restricted to the central regions in numerous frequency and glucagon-IR cells were evenly distributed in the mantle and peripheral regions in moderate frequencies, respectively. Somatostatin-IR cells were observed in the central and mantle regions in moderate and a few frequencies, respectively. In addition, hPP-IR cells showed similar distributional patterns to those of glucagon-IR cells except cells were also located in the central regions in rare frequency. In the exocrine portions, only glucagon- and hPP-IR cells were demonstrated in rare and a few frequencies, respectively. In conclusion, the regional distribution and relative frequency of pancreatic endocrine cells of the Korean aucha perch showed general patterns, which were observed in other teleost. However, some species-dependent different distributional patterns and/or relative frequencies were also demonstrated especially to serotonin-IR cells. In pancreas of the Korean aucha perch, insulin-IR cells were the most predominant cell type followed by glucagon-, somatostatin-, hPP- and serotonin-IR cells.