• Plant Biotechnology Reports
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• v.5 no.4
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• pp.301-308
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• 2011

This study tested the relative and combined efficacy of ShPx2 and ShPAL transgenes by comparing Nicotiana tabacum hybrids with enhanced levels of $_L$-phenylalanine ammonia lyase (PAL) activity and cationic peroxidase (Prx) activity with transgenic parental lines that overexpress either transgene. The PAL/Prx hybrids expressed both transgenes driven by the 35S CaMV promoter, and leaf PAL and Prx enzyme activities were similar to those of the relevant transgenic parent and seven- to tenfold higher than nontransgenic controls. Lignin levels in the PAL/Prx hybrids were higher than the PAL parent and nontransgenic controls, but not significantly higher than the Prx parent. All transgenic plants showed increased resistance to the necrotrophs Phytophthora parasitica pv. nicotianae and Cercospora nicotianae compared to nontransgenic controls, with a preponderance of smaller lesion categories produced in Prx-expressing lines. However, the PAL/Prx hybrids showed no significant increase in resistance to either pathogen relative to the Prx parental line. These data indicate that, in tobacco, the PAL and Prx transgenes do not act additively in disease resistance. Stacking with Prx did not prevent a visible growth inhibition from PAL overexpression. Practical use of ShPAL will likely require more sophisticated developmental control, and we conclude that ShPx2 is a preferred candidate for development as a resistance transgene.

• Journal of Plant Biology
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• v.37 no.3
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• pp.263-269
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• 1994

Involvement of ethylene and Ca2+ on the induction of phenylalanine ammonia-lyase (PAL) was investigated in Daucus carota L. suspension culture system. Ethylene production started to increase about 3 h after glucan treatment. And the maximal induction of ethylene was preceded by PAL induction by 30 min. After the treatment of ethrel, PAL activity was increased. When cells were treated with glucan and Co2+, PAL activity was simultaneously reduced. Ethylene production was reduced dramatically in calcium-free medium, even though glucan was treated. PAL activity and ethylene producton was inhibited conspicuously when ethylene glycolbis($\beta$-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA) was treated with glucan. Verapamil and trifluoperazine also inhibited PAL activity. When cells were treated with calcium ionophore A23187, PAL activity was increased in nontreated medium. We report here PAl activity is increased in related to ethylene production and involvement of Ca2+ in glucan-treated carrot suspension cells.

• Preventive Nutrition and Food Science
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• v.2 no.4
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• pp.354-359
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• 1997

Optimal cultivation conditions for the production of phenylalanine ammonia-lyase(PAL) from Rhodotorula aurantiaca K-505 were selected, and the kinetic parameters of the produced PAL were determined. The most suitable carbon and nitrogen sources were glucose and tryptone, respectively. The strain expressed PAL constituttively when using the optimized semi-complex media. High cell density culture could be critical for maximal production of PAl since the PAL ynthesis was growth associated. maximum PAL activity was observed at initial pH 6.0. although the ll growth was not markedly affected by temperature between 22 and 28$^{\circ}C$, the cells yielded the maximum PAL activity when cultivated at 22$^{\circ}C$. The maximum activity for deamination of L-phenylalnine to trans-cinnamic acid was observed around pH 8.8. The PAL activity gave the maximum at 45$^{\circ}C$, and greatly decreased at higher than 5$0^{\circ}C$. Activation energy({TEX}$E_{a}${/TEX}) calculated from Arrhenius equation was 6.28 kcal/mol in the range of 22$^{\circ}C$ to 4$0^{\circ}C$. A oolf plot showed that the enzyme reaction follows Michaelis-Menten equation, whose {TEX}$K_{M}${/TEX} and {TEX}$V_{max}${/TEX} values were 4.65$\times${TEX}$10^{-3}${/TEX} M and 0.89$\mu$ mol/mg-min respectively.

• Archives of Plastic Surgery
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• v.32 no.4
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• pp.496-502
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• 2005

This study was performed to compare the effect of external ultrasound assisted PAL(power assisted liposuction) with that of PAL alone. We performed 17 cases of liposuction from January 2003 to June 2003. For comparing both systems, we treated the right side(study group) with external ultrasound assisted PAL and the left side(control group) with PAL alone. To evaluate the difference in results between two groups, we surveyed three objective and four subjective items. Objectives were categorized as "Reduction rate of postoperative swelling", "Efficiency of suction" and "Histologic finding of aspirated fat tissue". Subjectives were also filed as "Degree of postoperative bruising", "Cannula resistance that operator felt", "Degree of postoperative pain" and "Patient's satisfaction". We assessed by questionnaire three times at 1day, 7 days and 30 days after operation. There were statistically significant differences in two of the subjective items; "Degree of postoperative pain at postoperative 1 day" and "Cannula resistance that operator felt". This study concludes that external ultrasound PAL is more effective high mobility of fat tissue and low tension of surrounding tissue. External ultrasound PAL will be more useful in case of large volume liposuction and revision procedures.

• Biomolecules & Therapeutics
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• v.17 no.1
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• pp.104-110
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• 2009

Phenylketonuria (PKU) is an inherited metabolic disorder caused by mutations in the phenylalanine catabolic enzyme, phenylalanine hydroxylase (PAH). The use of phenylalanine ammonia-lase (PAL) by oral and parenteral routes as a therapeutic drug for PKU has been severely limited due to inactivation by intestinal proteolysis and immune reactions. PEGylation was applied to PAL to reduce the degrees of antigenicity and proteolytic inactivation. Kinetic experiments with native PAL and pegylated PALs were performed, and pH stability, temperature stability, and protease susceptibility were evaluated. Enzyme linked immunosorbent assay (ELISA) was carried out to measure the immune complex between pegylated PALs and antiserum that had been extracted from a PAL-immunized mouse. Pegylated PAL, especially branched pegylated PAL (10 kDa, 1:32), was more active for phenylalanine and more stable in pancreatic proteases than native PAL. Native PAL was optimal at pH 8.5, corresponding to the average pH range of the small intestine; the same finding was noted for pegylated PALs. All linear and branched pegylated PALs had low reactivity with mouse antiserum, especially the 1:16 formulation with linear 5-kDa PEG and the 1:32 formulation with branched 10-kDa PEG. Therefore, we suggest the 1:32 formulation with branched 10-kDa PEG as the most promising formulation for enzyme replacement therapy.

• Journal of Korean Medical classics
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• v.10
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• pp.671-728
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• 1997

By studying from Chun Kuk are(戰國時代) to Chung area(淸代), I could find some conclusion as follows. 1. It was Chun Kuk area(戰國時代) that was shown some signs of forthcoming activity of theory of Ki Kyung Pal Maek(奇經八脈). In that time, Hwang Jae Nae Kyung(黃帝內經) had been written. That book made a important role for foundation of theory of Ki Kyung Pal Maek(奇經八脈), but it hadn't sufficient contents in some parts. 2. The theory of the Ki Kyung Pal Maek (奇經八脈) had been developed in Jin Han area(秦漢時代), Nan Kyung(難經) had been written in the area, Nan Kyung(難經) had been the first book which had referred to name and physiology of Ki Kyung Pal Maek(奇經八脈) and it also had mentioned about course, function, and pathology of that in simple manner. 3. In Yang Jin are and Su Dang area(兩晋 및 隋唐時代), Maek Kyung(脈經) and Hwang Jae Nae Kyung Tae So(黃帝內經太素) had been written Maek Kyung(脈經) had been the first book which had referred to pathology of Ki Kyung Pal Maek(奇經八脈) by Maek Jin(脈診) in detail, and it had affected on such books like Tae Pyung Seoung Hveu bang (太平聖惠方) and Ko Kum Yeu Tong Dae Chon(古今醫統大全). Hwang Jae Nae Kyung Tae So(黃帝內經太素) had mentioned about course, function, and pathology about Ki Kyung Pal Maek(奇經八脈) in most detail at that time, and it also had referred to that of such books like Myung Dang(明堂) and Ku Khoun Kyung(九卷經) which do not exist in present. Because of these point, Hwang jae Nae Kyung Tae So(黃帝內經太素) is evaluated an important book of theory of Ki Kyung Pal Maek(奇經八脈). 4. In Song area(宋代), Seung Jae Chong Rok(聖濟總錄) had been written. Seung Jae Chong Rok(聖濟總錄) first mentioned about meeting between Ki Kyung Pal Maek(奇經八脈) and Sip I Jong Kyung(十二正經), so this book helped development of theory of Ki Kyung Pal Maek(奇經八脈) of Kum Won area(金元時代). 5. In Kum Won area(金元時代), Dong In Su Hyul Chym Ku Do Kyung(銅人腧穴針灸圖經) had been written. Dong In Su Hyul Chym Ku Do Kyung(銅人腧穴針灸圖經) made a important role of theroy of Im Meak(任脈) and Dok Meank(督脈). Sip Sa Kyung Bal Hyui(十四經發揮) was superior to other books which had been referred to theory of Ki Kyung Pal Maek(奇經八脈) at that time. Also Sip Sa Kyung Bal Hyui(十四經發揮) more mentioned about meeting between Ki Kyung Pal Maek(奇經八脈) and Sip I Jong Kyung(十二正經) than that of Seung Jae Chong Rok(聖濟總錄), so this book helped dvelopment of theory of Ki Kyung Pal Maek(奇經八脈). 6. In Myung area(明代), Ki Kyung Pal Maek Ko(奇經八脈考) that had almost accomplished theory of Ki Kyung Pal Maek(奇經八脈) had been written. Ki Kyung Pal Maek Ko(奇經八脈考) was the best book more than any other book that had been mentioned about theory of Ki Kyung Pal Maek(奇經八脈) in three ways as follows. 1) It is Ki Kyung Pal Maek Ko(奇經八脈考) that mentioned about near similar manner of modern course and physiology of Ki Kyung Pal Maek(奇經八脈). 2) Ki Kyung Pal Maek Ko(奇經八脈考) mentioned about pathology and treatment of Ki Kyung Pal Maek(奇經八脈) in detail more than any other book. 3) Ki Kyung Pal Maek Ko(奇經八脈考) emphasized on relationship between Ryun Kong(鍊功) and Ki Kyung Pal Maek(奇經八脈).

• Journal of Environmental Science International
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• v.14 no.12
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• pp.1093-1102
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• 2005

Effects of abscisic acid(ABA) and temperature on the anthocyanin accumulation and phenylalanine ammonia Iyase(PAL) activity were investigated in seedlings of Arabidopsis thaliana. In time course study, exogenous application of ABA $(50-1000\;{\mu}M)$ led to a noticeable increase in anthocyanin pigments which persisted over the following 5 days. Anthocyanins increased in concert with the chlorophyll loss. The activity of PAL, a key enzyme in the phenylpropanoid pathway, increased on exposure to ABA and reached maximum on the 4th day, This result shows that anthocyanin synthesis and PAL activity have a close physiological relationships. In the effects of temperatures ($10^{\circ}C,\;17^{\circ}C,\;25^{\circ}C$and $30^{\circ}C$) on anthocyanin accumulation and PAL activity in seedlings, a moderate-low temperatures ($17^{\circ}C$) enhanced both anthocyanin content and PAL activity, whereas elevated temperatures ($30^{\circ}C$) showed low levels of anthocyanin and PAL activity, suggesting a correlation between temperature-induced anthocyanin synthesis and the accumulation of PAL mRNA. Simultaneous application of ABA with temperatures Induced higher anthocyanin synthesis and PAL activity in seedlings than ABA or temperature stress alone. Moderate-low temperature with ABA exposure elicited the maximal induction of anthocyanin synthesis and PAL activity. Therefore, ABA treatment significantly increased thermotolerance in .A. thalinan seedlings. Ethephon and ABA showed similar mode of action in physiological effects on anthocyanin accumulation and PAL activity. Our data support that anthocyanins may be protective in preventing damage caused by environmental stresses and play an important role in the acquisition of freezing tolerance.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.2
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• pp.101-107
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• 2005

To investigate the effect of pyroligneous acid liquor (PAL) on the maturity of pig manure compost, PAL was treated to the compost piles. The treatments included applications of 100 and 300 times diluted PAL in addition to the control. The compost piles were stirred in three times at the 1st, 10th, and 25th day of composting. Temperature in the compost pile of control treatment increased from 28 to $60^{\circ}C$ within 10 days and remained nearly at the level until 30th day, then it began to decrease. On the other hand, temperature of the PAL-100 and PAL-300 treatments reached 65 to $70^{\circ}C$ within 8 days and became stabilized until 30th day, then the temperature decreased to about $28^{\circ}C$. However, the temperature of control was stabilized approximately at the 40th day. Initially, the pH of control treatment dropped from 8.2 to slightly above pH 8.0 during 4th day, but that of the PAL-100 treatment declined to 7.8. Among treatments, pH value of PAL-100 treatment was the lowest, which was about 7.3 after becoming stabilization. Also the germination index (GI) was increased at all treatments. The C/N ratio range of PAL-100 treatment was better balanced than others and was at 24.3. Moreover the round paper chromatogram of extracted solution of compost of PAL-100 treatment was the sharpest and clearest among treatments. The GI values of control, PAL-100, and PAL-300 in 60 days of composting were about 108, 120, and 118 in germination test using chinese cabbage, respectively. It can be concluded that the addition of diluted PAL solution is effective in composting of pig manure.

• Proceedings of the Korean Vacuum Society Conference
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• 2009.08a
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• pp.335-335
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• 2009

• Korean Journal of Microbiology
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• v.26 no.3
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• pp.270-277
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• 1988

Microorganisms isolated from soil (150 strains), fungi (39 strains), yeasts (9 strains) and Streptomyces species (39 strains) were assayed for L-phenylalanine ammonia-lyase(PAL) activity. 17 strains of fungi and 46 strains of soil isolates were proved to produce PAL, Aspergillus panamensis, Penicillium varioti and 11 soil isolates showed comparatively large PAL activity. When PAL activity was assayed with cell-free extracts of these 13 strains and 7 strains of Rhodotorula and Rhodosporidium geni, Rhodosporidium toruloides (IFO 0559) showed the highest PAL activity with 0.333 units per g of the wet cell weight.