• Title/Summary/Keyword: pak-choi

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Effect of Surfactant Addition on Se Absorption and Growth of Pak-choi and Leaf Lettuce in Hydroponics (수경 재배 시 계면 활성제 첨가가 상추와 청경채의 생육과 Se 흡수에 미치는 영향)

  • Yuna, Hyung-Kwon;Seo, Tae-Cheol;Zhang, Cheong-Hao;Chun, Chang-Hoo
    • Journal of Bio-Environment Control
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    • v.18 no.2
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    • pp.124-131
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    • 2009
  • Surfactant polyvinyl alcohol (PVA-95) 1, 2, 4mg. $L^{-1}$ and calcium lignosulfonate (CLS) 5, 25, 50, 100mg. $L^{-1}$ were treated to the nutrient solution containing 1.0mg. $L^{-1}$ selenium (Se) to evaluate Se absorption effect using small DFT apparatus. The growth of leaf lettuce and pak-choi did not show unique significance by surfactant kinds and concentration in the tested range and physiological disorder. Vitamin C in leaves of two leafy vegetables was not significantly affected by surfactants also. Among major cations K content in pak-choi was highest in Se I+PVA-95 4mg. $L^{-1}$ treatment, and high in Se I+CLS 5 mg. $L^{-1}$ treatment. Ca content in two leafy vegetables showed increasing pattern by the two kinds of surfactants. Mg content was high in Se I+CLS 25mg. $L^{-1}$ treatment compared to control. Pak-choi absorbed Se about 10 times higher than leafy lettuce. Se content in leaves of two leafy vegetables increased by the treatment of two kinds of surfactants with the exception of CLS 100mg. $L^{-1}$ treatment.

Production of Monoclonal Antibody about Specific Key Enzyme of Hyoscyamine $6{\beta}-Hydroxylase$ (H6H) in Scopolia parviflora

  • Kang, Young-Min;Jung, Hee-Young;Kang, Seung-Mi;Jin, Byung-Rae;Lee, Sang-Chul;Lee, Byung-Hyun;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.2
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    • pp.135-140
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    • 2004
  • Total RNAs were isolated from cultured roots of Scopolia parviflora, $poly(A)^+$ RNA was obtained through the mRNA purification, cDNA library of Hnh6h was constructed. Recombinant baculoviruses in Spodoptera frugiperda (Sf) cells were constructed by use of the transfer vector pBacPAK, which has the AcNPV sequence under the polyhedrin promoter. The expression vector carrying Hnh6h gene was transferred to S. parviflora and obtained transgenic hairy root lines. Our results confirmed the over expression of the H6H protein was used by anti-pBacPAK about cDNAs of S. parviflora. This study will served for production of tropane alkaloids by metabolic engineering.

Analysis of Carbendazim and Carbaryl by Micro-HPLC (Micro-HPLC에 의한 Carbendazim과 Carbaryl의 분석)

  • Choi, Kyoung-Sook;Lee, Hoe-Yeon;Lee, Duck-Hee;Byun, Woon-Ki
    • Analytical Science and Technology
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    • v.7 no.3
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    • pp.421-425
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    • 1994
  • A micro-high performance liquid chromatography(Micro-HPLC) techniquie with solid phase extraction was reported which detected carbendazim and carbaryl at picogram levels. They were separated on microbore packed $C_{18}$ column($1.0mm\;I.\;D{\times}150mm$, $d_f=5{\mu}m$) using a 50% methanol mobile phase and detected at UV 220nm(${\alpha}=2.94$, $R_s=4.71$), while they were not resolved on analtical HPLC system(${\alpha}=1.27$, $R_s=0.76$). The detection thresholds of carbendazim and carbaryl were 0.5ng and 0.1ng on Micro-HPLC, therefore Micro-HPLC system was 20~40 told more sensitive than anayltical HPLC system. Sep-Pak $C_{18}$ catridge was found to be efficient in enriching carbendazim and carbaryl from dilute aqueous solution with 97.0% and 97.8% recoveries of them. The Sep-Pak $C_{18}$ catridge followed by the Micro-HPLC had been applied to the quantitative analysis of carbendazim and carbaryl in spiked juices and a commercial drinking water.

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Compartment Syndrome and Deep Vein Thrombosis after Repetitive Posterior Cruciate Ligament Reconstruction (반복된 후방 십자 인대 재건술 후 발생한 구획 증후군 및 심부 정맥 혈전증)

  • Kim, Jin-Goo;Lee, Soo-Won;Choi, Hong-Joon
    • Journal of the Korean Arthroscopy Society
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    • v.12 no.1
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    • pp.58-62
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    • 2008
  • Posterior cruciate ligament reconstructions are dangerous procedure in terms of neurovascular complication. But deep vein thrombosis and associated compartment syndrome after posterior cruciate ligament reconstruction has not been reported, yet. We have experienced a patient who developed a deep vein thrombosis and compartment syndrome after revision posterior cruciate ligament reconstructive surgery, and report the importance of diagnosis and management with the discussions.

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