• Archives of Pharmacal Research
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• v.17 no.5
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• pp.360-363
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• 1994

Column--swtiching technique with a reversed-phase high performance liquid chromatographic method has been developed for the routine analysis of radioiodinated insulin and its degadation products in biological fluids. The diluted biological samples were loaded onto a precolumn packed with LiChrosorb RP-8 $(25-40{\;}{\mu}m)$ using 0.1% trifuoroacetic acid (TFA) in water as a washing solvent. After valve switching, the concentrated insulins were eluted in the back-flush mode and separated by a W-Porex $C_{18}$ column with a gradient of 0.1% TFA in water and 0.1% TFA in acetonitrile as the mobile phase. The method showed good precision, accuracy and speed with the detection limit of 20 pg/ml. Total analysis time per sample was about 40 min and the coefficients of variation were less than 8, 2%.

• KSBB Journal
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• v.10 no.5
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• pp.482-490
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• 1995

The relationship between pressure drop and liquid flow rate, for an axial and a radial flow chromatographic column packed with compressible porous media was theoretically analyzed using modified Kozeny-Carman equation. The results were compared with experimental observations obtained using compressible DEAE-agarose as a model medium. At 2-9 psi range studied, the theoretical derivation accounting for 'gel compression' effect predicted simple Langmuirian type response of volumetric flow rate to changes in pressure drop. On the other hand, the experimental response was more or less sigmoidal. At the same pressure drop, radial column showed 2-3 times higher flow rates than those of axial column both theoretically and experimentally. Using r-HBsAg crude extract, protein resolution effects between the two types of columns at different flow rates were compared side-by-side. It turned out that, though general chromatographic behavior was very similar, axial column was somewhat superior in terms of r-HBsAg recovery yield and specificity. However, the number of theoretical plates analysis indicated the protein resolution effects were comparable.

• Applied Biological Chemistry
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• v.35 no.2
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• pp.132-138
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• 1992

For effective determination of methylthiohydantoin amino acids(MTHs) by gas liquid chromatography in the protein sequencing, derivatization with N-methyl-N-(tert.-butyl-dimethylsilyl)trifluoroacetamide(MTBSTFA), a new silylating reagents, was attempted instead of trimethylsilyl(TMSi) derivatives by N,O-bis(trimethylsilyl)trifluoroacetamide(BSTFA) used up to the present and N(O)-butyldimethylsilyl MTHs derivatized by MTBSTFA were analysed on HP-1 capillary column. Twenty one protein amino acids except cystine were indentified. Especially arginine that did not detected with TMSi derivative on packed column until now was resolved by derivatization with MTBSTFA. N(O)-butyldimethylsilyl MTHs showed multiple peaks by MTBSTFA were proline, isoleucine, glycine and tyrosine and hydroxyproline especially showed several extraneous peaks more than two. Calibration curves of N(O)-butyldimethysilyl MTHs of amino acids in the range of $2.5\;nmol{\sim}7.5\;nmole$ showed good linearity. however, those of lysine, histidine and arginine showed linearity in the range of $5.0\;nmole{\sim}15.0\;nmole$. Correlation coefficients and regression coefficients of all calibration curves were highly significant(p<0.001).

• Mass Spectrometry Letters
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• v.2 no.3
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• pp.65-68
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• 2011

The effects of column length and particle size on the efficiency of separation and characterization of phospholipids (PLs) are investigated using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nLC-ESI-MS-MS). Since PLs are associated with cell proliferation, apoptosis, and signal transduction, it is of increasing interests in lipidomics to establish reliable analytical methods for the qualitative and quantitative profiling of PLs related to biomarker development in adult diseases. Due to the complexity of PLs, the preliminary separation of PLs is necessary prior to MS analysis. In this study, length of capillary column and the particle size of reversed phase ($C_{18}$) packing materials are varied to find a reliable condition for the high speed and high resolution separation using 8 PL standard mixtures. From experiments, it was found that a capillary column of nLC-ESI-MS-MS analysis for PL mixtures can be minimized to a 5 cm long pulled tip column packed with 3 ${\mu}m$ $C_{18}$ particles without losing resolution.

• Analytical Science and Technology
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• v.7 no.3
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• pp.421-425
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• 1994

A micro-high performance liquid chromatography(Micro-HPLC) techniquie with solid phase extraction was reported which detected carbendazim and carbaryl at picogram levels. They were separated on microbore packed $C_{18}$ column($1.0mm\;I.\;D{\times}150mm$, $d_f=5{\mu}m$) using a 50% methanol mobile phase and detected at UV 220nm(${\alpha}=2.94$, $R_s=4.71$), while they were not resolved on analtical HPLC system(${\alpha}=1.27$, $R_s=0.76$). The detection thresholds of carbendazim and carbaryl were 0.5ng and 0.1ng on Micro-HPLC, therefore Micro-HPLC system was 20~40 told more sensitive than anayltical HPLC system. Sep-Pak $C_{18}$ catridge was found to be efficient in enriching carbendazim and carbaryl from dilute aqueous solution with 97.0% and 97.8% recoveries of them. The Sep-Pak $C_{18}$ catridge followed by the Micro-HPLC had been applied to the quantitative analysis of carbendazim and carbaryl in spiked juices and a commercial drinking water.

• Journal of Soil and Groundwater Environment
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• v.16 no.3
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• pp.10-16
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• 2011

The experimental studies for remediation of diesel contaminated soils were performed using subcritical water in laboratory scale. Contaminated soils from industrial area and artificially contaminated soils were utilized for soil remediation. Experimental system was composed for subcritical water to flow upward through the soil packed column for extracting contaminants. 10 g of contaminated soil was packed into the column and water flow rate was 2 mL/min. To evaluate the effects of temperature, pressure and treatment time on the removal efficiency, temperature was changed from 100$^{\circ}C$ to 350$^{\circ}C$, pressure from 50 bar to 220 bar and treatment time at the predetermined temperature from 0 min to 120 min. The purification efficiency increased as temperature increased. However, the effect of pressure and treatment time was low. Temperature 250$^{\circ}C$, pressure 50 bar and treatment time 30 min were selected for optimal operating condition for this study.

• Nuclear Engineering and Technology
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• v.41 no.10
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• pp.1275-1284
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• 2009

A directly heated $SO_3$ decomposer for the sulfur-iodine and hybrid-sulfur processes has been introduced and analyzed using the computational fluid dynamics (CFD) code CFX 11. The use of a directly heated decomposition reactor in conjunction with a very high temperature reactor (VHTR) allows for higher decomposition reactor operating temperatures. However, the high temperatures and strongly corrosive operating conditions associated with $SO_3$ decomposition present challenges for the structural materials of decomposition reactors. In order to resolve these problems, we have designed a directly heated $SO_3$ decomposer using RA330 alloy as a structural material and have performed a CFD analysis of the design based on the finite rate chemistry model. The CFD results show the maximum temperature of the structural material could be maintained sufficiently below 1073 K, which is considered the target temperature for RA 330. The CFD simulations also indicated good performance in terms of $SO_3$ decomposition for the design parameters of the present study.

• Bulletin of the Korean Chemical Society
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• v.31 no.4
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• pp.905-909
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• 2010

Magnetic beads (Dynabeads$^{(R)}$) embedded in ~1 micron size polystyrene beads bearing surface carboxylic acid groups were modified with aminobenzyl ethylenediaminetetraacetic acid (ABEDTA) to concentrate or separate metal ions using pH gradients on micro and nano scales. The immobilization of ABEDTA was achieved by amide formation. The presence of the metal chelating functional group in the fully deprotonated form was confirmed by FT-IR. The chelation efficiency of beads was tested by determining metal ions in supernatant using GFAAS when pH gradients from 3 to 7. Mixtures of Cu and Mg and of Cd and Mn (at 10 ng/mL of metal) were separated as the difference in formation constant with the functional group of ABEDTA. The separation was repeated twice with relative standard deviation of <18%. A polydimethylsiloxane (PDMS) microchip column packed with EDTA-coated magnetic beads was optimized to concentrate metal ion for practical applications by eluting a Cu solution of micro scale at pH 3.

• Journal of Korean Society of Water and Wastewater
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• v.33 no.2
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• pp.131-140
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• 2019

This study used a packed column reactor and a horizontal flow mesh reactor to examine the removal of copper ions from aqueous solutions using pine bark, a natural adsorbent prepared from Korean red pine (Pinus densiflora). Both equilibrium and nonequilibrium adsorption experiments were conducted on copper ion concentrations of 10mg/L, and the removals of copper ions at equilibrium were close to 95%. Adsorption of copper ions could be well described by both the Langmuir and Freundlich adsorption isotherms. The bark was treated with nitric acid to enhance efficiency of copper removal, and sorption capacity was improved by about 48% at equilibrium; mechanisms such as ion exchange and chelation may have been involved in the sorption process. A pseudo second-order kinetic model described the kinetic behavior of the copper ion adsorption onto the bark. Regeneration with nitric acid resulted in extended use of spent bark in the packed column. The horizontal flow mesh reactor allowed approximately 80% removal efficiency, demonstrating its operational flexibility and the potential for its practical use as a bark filter reactor.

• 한국태양에너지학회:학술대회논문집
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• 2009.11a
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• pp.404-408
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• 2009

The liquid desiccant air-conditioning system has been proposed as an alternative to the conventional vapor compression cooling systems to control air humidity. The complete system of liquid desiccant air-conditioning system is consisted two main components those are humidifier (regeneration) and dehumidifier. Humidifier part is connected to the load when summer season which is the air condition is hot and humid have to be turned into comfort condition on human. This paper purpose is performances study of air flow rate effect on a structured packed tower on cooling and dehumidifier system using liquid lithium chloride as the desiccant. Experimental apparatus used in this present study is consisted of three components those are load chamber, packed tower and chiller. Load chamber’s volume is $40m^3$, and packed tower dimension is cubic with length 0.4m occupied with packed column. Totally, 15 experimental has done using 5 times repeat on each variable of air velocity that varying on 2m/s, 3m/s and 4m/s with other conditions are controlled. Air inlet initial temperature and relative humidity are set respectively on $30^{\circ}C$ and 52%, desiccant flow rate is 0.63 kg/s, desiccant temperature is $10^{\circ}C$ and desiccant concentration is 0.4. The result of this study shows that averagely, the moisture removal rate and the heat transfer rate are influenced by the air velocity. Higher air velocity will increase the heat transfer and decreasing the moisture removal rate. At adiabatic condition the air velocity of 2 m/s respectively is having the higher moisture removal rate acceleration then the air velocity of 3m/s and 4 m/s until the steady state condition.