• 미생물학회지
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• 제40권2호
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• pp.172-177
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• 2004

광합성 박테리아 Rhodospirillum rubrum은 광원의 조건에 따라 균주 내의 신진대사 양상이 변화된다고 보고 되어 왔다. 이러한 광조건에 의한 신진대사 변환 과정을 담당하는 유전자에 대한 연구는 광합성 박테리아에 관한 주된 과제 중 하나이다. 근래 이러한 광조건에 의한 균주내 변화 과정이 균주가 지니는 extrachromosomal plasmid와 관련이 있음이 보고되었다. 본 연구는 광합성 박테리아 R. rubrum의 extrachromosomal plamid pKYl 을 분리 정제하고 이를 제한효소 HindIII로 단편화 하여 이들 단편의 일부에 대한 염기서열을 분석하고 이들의 기능을 추론하였다. 본 연구를 통하여 plasmid pKY1에 박테리아의 유전적 재조합 (genetic recombination)에 관여하는 단백질에 대한 정보가 위치하고 있음을 유전자 및 아미노산 상동성 조사를 통하여 알 수 있었다.

• 한국식품영양과학회지
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• 제5권1호
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• pp.75-80
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• 1976

우모(牛毛) 및 모발을 단백질자원(蛋白質資源)으로 이용하고자 그 처리 가공방법(加工方法)을 실험하여 얻은 결과를 요약하면 다음과 같다. (1) 알칼리 분해(分解)는 NaOH 1% 용액(溶液)에 우모(牛毛) 및 모발을 10% 투입하여 $100^{\circ}C$에서 $25{\sim}30$분(分) 가열 분해(分解)하면 팽윤하여 연화분해(軟化分解)된다. (2) 이것을 humin질(質), fiber 등 불순물과 이물(異物)에서 단백질(蛋白質)을 분리(分離)해 내는 데는 용해도차(差) 및 등전점(等電點) pH를 이용하여 분리단백질(分離蛋白質)을 만들 수 있었다. (3) 분리단백질(分離蛋白質)은 pH $3{\sim}4.5$의 등전점(等電點) pH 를 가졌고 이의 조성(組成)은 대부분이 단백질(蛋白質)로 되어 있고 amino acid조성도 비교적 좋아서 단백질(蛋白質)로 이용할 만했다. (4) 산분해(酸分解)는 20%의 HCl, $H_2SO_4$ 용액에 액량(液量)의 30%의 keratin을 투입하여 분해시킴이 좋았고 불순물은 용액도차(溶液度差) 및 흡착제로 제거할 수 있었다. (5) 이때의 amino acid concentrate는 분리단백질(分離蛋白質)과 같이 몇 종의 필수아미노산을 안가(安價)로 강화(强化)시키면 훌륭한 단백질자원(蛋白質資源)으로 활용(活用)할 수 있을 것으로 사료된다.

• 치과방사선
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• 제24권2호
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• pp.291-304
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• 1994

The purpose of this study was to analyze the facial asymmetry of the patients with the craniomandibular disorder. In this study, 50 patients, who have joint clicking and pain, mouth opening limitation, and 40 dental students, Chosun University, who did not posses any restoration and orthodontic treatment, joint clicking and pain, mouth opening limitation, were selected as the control group. Both the control group and the patient group were takened skull P-A, submento-vertex radiogram by standized methods. After that, the deviation and facial asymmetry were measured and analyzed. The results of the this study were as follows: 1. In the Skull P-A radiogram, the width difference of control group and patient group measured that the △ Cg-Go-Cl: control group were 3.35㎜, patient group were 4.51㎜ (P<0.05), the △Cg-Zy-Go: control group were 1.83㎜, patient group were 3.27㎜(P<0.001). 2. In the Skull P-A radiogram, the height difference of control group and patient group measured that the △ Cg-Go-Cl: control group were 131.85㎜, patient group were 188.45㎜(P<0.05), the △Cg-Zy-Go: control group were 1.58㎜, patient group were 2.68㎜(P<0.00l). 3. In the Skull P-A radiogram, the area difference of control group and patient group measured that the △ Cg-Go-Cl: control group were 120.76㎟, patient group were 185.49㎟(P<0.05), the △Cg-Zy-Go: control group were 2.29㎟, patient group were 3.37㎟(p<0.05). 4. In the submento-vertex radiogram, the width difference of control group and patient group measured that the △Mr-Cl-Ia: control group were 1.50㎜, patient group were 2.35㎜(P<0.05), the △Mr-Cm-Ia: control group were 1.75㎜, patient group were 3.17㎜(P<0.05), the △Mr-Go-Ia: control group were 1.96㎜, patient group were 3.24㎜(P<0.001), the △Mr-Cp-Co: control group were 1.74㎜, patient group were 2.73㎜(P<0.05). 5. In the submento-vertex radiogram, the height difference of control group and patient group measured that the △Mr-Cp-Ia: control group were 1.68㎜, patient group were 2.46㎜P<0.05), the △Mr-CI-Ia: control group were 2.38㎜, patient group were 3.74㎜(P<0.05), the △Mr-Co-Ia: control group were 1.63㎜, patient group were 2.80㎜(P<0.05), the △Mr-Cm-Ia: control group were 1.45㎜, patient group were 3.12㎜(P<0.001). 6. In the submento-vertex radiogram, the area difference of control group and patient group measured that the △ Mr-Cp-Ia: control group were 73.17㎟, patient group were 110.16㎟(P<0.05), the △Mr-Cl-Ia: control group were 105.09㎟, patient group were 180.87㎟(P<0.001), the △Mr-Co-Ia: control group were 103.31㎟, patient group were 148.48㎟(P<0.05), the △Mr-Cm-Ia: control group were 97.01㎟, patient group were 167.83㎟(P<0.05), the △Mr-Go-Ia: control group were 104.24㎟, patient group were 205.90㎟(P<0.05).