• Title/Summary/Keyword: pH change

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Production of Mozzarella Cheese Analogue by Ultrafiltration (한외여과를 이용한 Mozzarella Cheese Analogue 제조에 관한 연구)

  • Lee, Sung-Hui;Song, Kwang-Young;Seo, Kun-Ho;Yoon, Yoh-Chang
    • Journal of Dairy Science and Biotechnology
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    • v.31 no.1
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    • pp.21-33
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    • 2013
  • The purpose of this study was to improve the production of Mozzarella cheese analogues manufactured using mixtures of soy milk and concentrated raw milk by performing ultrafiltration (UF) and to assess the quality of these cheeses during a 30-day storage period at $4^{\circ}C$, relative to that of Mozzarella cheese manufactured with the traditional method. The solid consistency of Mozzarella cheese analogue prepared from milk mixtures was lower than that of cheese manufactured from raw milk or soy milk and increased during storage, which is considered to be the result of decreasing water levels, as well as with increasing soy milk concentrations. In the Mozzarella cheese analogue generated using the milk mixtures, the fat content decreased with increase in the soy milk concentration, while it decreased during the storage period. Lactose levels were lowest in cheese composed of soy milk or raw milk and processed by UF, and decreased during storage in cheese produced using milk mixtures. In milk mixtures containing soy milk, the protein concentration increased with increasing amounts of raw milk and did not change during the storage period. The water-soluble nitrogen compound level was similar between cheeses and increased only slightly during storage. The amount of non-protein nitrogen compounds was higher in the cheese analogue than in the control cheese and tended to increase during storage. Analysis of the physicochemical traits of the Mozzarella cheese analogue yielded the following results: During storage, titratable acidity levels increased while pH tended to decrease. After analysis using electropherograms, it was classified as ${\alpha}$-, ${\beta}$-, or ${\kappa}$-casein. The results of rheometry tests showed that in the Mozzarella cheese analogue prepared from milk mixtures, with raw milk concentrated by UF, increases in concentration rate lead to lowered hardness, elasticity, cohesiveness, and brittleness. When cheese was produced from milk mixtures and concentrated by UF, meltability increased as the concentration rate increased, although to an extent that was less than that observed for the control cheese, and tended to increase during storage. Sensory evaluation showed that the analogue cheese was much better than the control cheese in terms of formation, appearance, and flavor.

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Effects of Stocking and Removal of Silver Carp on Plankton Communities in a Shallowhypertrophic Lake (부영양호의 enclosure내에서 어류의 밀도조절이 수질 및 플랑크톤 군집에 미치는 영향)

  • Choi, Min-Kyu;Hwang, Su-Ok;Noriko, Takamura;Kim, Baik-Ho
    • Korean Journal of Ecology and Environment
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    • v.33 no.4 s.92
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    • pp.358-365
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    • 2000
  • To evaluate the influences of stocking and removal of silver carp on plankton communities and physicochemical parameters, fish biomanipulation was conducted in six enclosures constructed on the coast of a hypertrophic lake during the summer of 1997. Of these, three enclosures were established for the fish-stocking experiment; one fishless enclosure used as a control and two enclosures with high and low densities of fish. The other three enclosures for the fish-removal experiment were prepared in an entirely converse process compared to that for fish stocking. The results of randomized intervention analysis (${\alpha}=0.05$) showed significant changes in several physicochemical parameters and plankton communities after fish stocking and removal. Fish stocking decreased water transparency, DO, pH and chi-a (> $40\;{\mu}m$), while increasing the concentration of nitrogen and chi-a (<$2\;{\mu}m$). However, water transparency did not increase by fish removal. Small plankton communities, such as picocyanobacteria, eukaryotic picoplankton and ciliates (<$2\;{\mu}m$) showed significant reciprocal changes after both fish treatments: increase by the presence of fish and decrease by the absence of fish. No count of large sized phytoplankton and zooplankton showed significant change in the presence of fish, while large zooplankton, Diaphanosoma, Cyclopida and nauplii were significantly increased by fish removal. Although the frequent heavy-winds and endless supply of phosphorus from the lake sediment strongly disturbed the fish behavior and enclosure management, the stocking and removal of silver carp showed an obvious reciprocal influences on plankton communities and its physico-chemical parameters in a shallow-hypertrophic lake.

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Performance Evaluation of Substitution Radiopharmaceutical according to Restriction of the Radiocolloids in Lymphoscintigraphy (Lymphoscintigraphy에 이용되는 방사성의약품의 사용제한으로 인한 대체 방사성의약품의 성능평가)

  • Seo, Young-Deok;Jeong, Jae-Hun;Moon, Jong-Woon;Yun, Sang-Hyeok;Kim, Yeong-Seok;Kang, Se-Hun;Won, Woo-Jae;Kim, Seok-Ki
    • The Korean Journal of Nuclear Medicine Technology
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    • v.13 no.1
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    • pp.25-29
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    • 2009
  • Purpose: Several radioactive agents were used for lymphoscintigraphy in breast cancer. But recently, due to the change of local radiopharmaceutical licensing policy and discontinuation of supplies from manufacturer, we could not use many radiocolloids such as $^{99m}Tc$-Antimony Trisulfide Colloid, $^{99m}Tc$-Tin Colloid, $^{99m}Tc$-Human Serum Albumin. So it is necessary to use the substitution radiopharmaceutical. Therefore, this study aims to evaluate the performance of substitution radiopharmaceutical ($^{99m}Tc$-Phytate) including the existing radiocolloids and to set up of the appropriate protocol in lymphoscintigraphy. Materials and Methods: For each radiocolloids ($^{99m}Tc$-Antimony Trisulfide Colloid (ASC), $^{99m}Tc$-Tin Colloid (TC), $^{99m}Tc$-Human Serum Albumin (HSA), $^{99m}Tc$-Phytate) were performed the particle size by Zeta Sizer (Nano-ZS) and we compared the radiolabeling time, procedure and acquisition time for each of the radiocolloids (total 200 patients). For the last time, we made an analysis of image for each of the radiocolloids with our previous report (SJ Jang et al, Korean Nucl Med Mol imaging Vol.41, No.6, Dec 2007). Results: The particle size of each radiocolloids showed A.S.C (50 nm), T.C (310 nm), H.S.A (10.8 nm), $^{99m}Tc$-Phytate (499 nm). The labeling and acquisition time for each of the radiocolloids showed no substantial difference. But there is difference of the labeling time for the A.S.C. because the ASC procedure need to boiling process. There were no significant differences among those radiocolloids (p>0.005) in the identification rate (IR), false negative rate (FNR), and negative predictive value (NPV). Conclusions: $^{99m}Tc$-labeled radiocolloids showed equivalent results in lymphoscintigraphy. Therefore, in this exam of each radiocolloids could be applied appropriate protocol in lymphoscintigraphy.

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Changes of Soil Physico-Chemical Properties under Different Tillages of Paddy Soil (경운방법(耕耘方法)에 따른 논 토양(土壤)의 이화학성(理化學性) 변화(變化))

  • Yoo, Chul-Hyun;Shin, Bog-Woo;Jeong, Ji-Ho;Han, Sang-Soo;Kim, Seong-Jo;Han, Seong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.2
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    • pp.140-145
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    • 1997
  • The change of physico-chemical properties of paddy soil was monitored under different tillage. This study was conducted on Jeonbug silty cly loam, in Honam Agricultural Experiment Station, for four years starting from 1992. Different tillage involved (1)Continued no tillage, (2)Rotavation only with tractor every year, (3)Spring plowing with power tiller plus rotavation with tractor every year. The result of this study can be summarized as: Non tillage, when continued for some years, tended to increase 1)the hardness of soil, 2)the root density in the top soil, 3)the occurrence of annual and perennial weeds, and tended to lower the yield of rice compared to normal tillage(tilling with ow tiller in the spring plus rotavation by tractor before planting.

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Vegetation Structure and Distributional Characteristics of Abies koreana Forests in Mt. Halla (한라산 구상나무림의 식생구조와 분포 특성)

  • Song, Kuk-Man;Kim, Chan-Soo;Koh, Jung-Goon;Kang, Chang-Hun;Kim, Moon-Hong
    • Journal of Environmental Science International
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    • v.19 no.4
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    • pp.415-425
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    • 2010
  • The purpose of the present study was to analyze the vegetation structure and distributional characteristics of Abies koreana forests in Mt. Halla, and to provide basicdata for an ecological study on Abies koreana in Mt. Halla. The results of the analysis showed that the mean importance percentage(M.I.P,) of Abies koreana in the Youngsil and Bangaeoreum and the Azalea field were 28.3%, 23.6%, and 46.4%, respectively. The ratios of DBH (diameter at breast height) to height were similar in all region, except in the Azalea field, where Abies koreana of various ages, both young and old, were found. The species diversity (H) of the upper and lower layers in the Youngsil and Bangaeoreum and in the Azalea field were 0.625 and 0.810, 0.731 and 0.848, and 0.342 and 0.757, respectively. A total of 52 community were distributed at locations higher than 1,300m above sea level. The proportions of each community in the whole Abies koreana forest were 56.5%(Azalea field), 11.0% (Youngsil trail at 1,550-1,650 m above sea level), and 8.1%(Janggumok and Kundurewat region). The total area of the Abies koreana forest was calculated to be 795.3ha by combining all the areas of each community. An Abies koreana forest with the largest area was found at locations 1,500-1,600 m above sea level, taking up 38.8% of the total Abies koreana forest area. For the slopes of the distributional area of Abies koreana, 46.1%(highest proportion) of the total area was $10\sim25^{\circ}$, and for the azimuth of the distributional area, 17.4%(the highest proportion) of the total area was $0-45^{\circ}$. The vegetation structure showed large differences between areas. It was found, however, that the distribution was mostly in the areas with a relatively gentle slope. It is suggested that research be done to forecast the possible changes in the differences in the vegetation structures between different areas caused by climate changes. In addition, there is a need to monitor the Abies koreana and alpine plants in the subalpine zones of Mt. Halla, which are sensitive to climate change, to obtain the basic data that are necessary for the protection and maintenance of the ecosystem.

UTILIZATION OF ROUGHAGE AND CONCENTRATE BY FEEDLOT SWAMP BUFFALOES (BUBALUS BUBALlS)

  • Wanapat, M.;Wachirapakorn, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.3
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    • pp.195-203
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    • 1990
  • Thirty-six yearling (18 bulls, 18 heifers) swamp buffaloes (Bubalus bubalis) with average liveweight $177{\pm}26kg$, were randomly allotted to receive 6 dietary treatments according to a $2{\times}3$ factorial arrangement in a completely randomized design (CRD). Factor A assigned for two types of straw; untreated rice straw (RS) and urea-treated (5%, w/w) rice straw (UTS). Factor B assigned for three ratio of roughage to concentrate (R:C) at 80:20, 50:50 and 20:80. Feeding trial lasted for 9 months during which respective feeds were offered at 3% body weight and adjusted at bi-weekly intervals using corresponding liveweights. Parameters measured under this experiment were feed intake, ruminal fluid for pH, $NH_3-N$, volatile fatty acids (VFA), liveweight change at bi-weekly intervals, carcass characteristics and cost-net profit analysis. It was found that intakes and digestion coefficients of DM, OM, CP except NDF and ADF were improved appreciably when ratio of concentrate increased. The average daily gain (ADG) and feed conversion ratio (FCR) were highest in group fed urea-treated rice straw at R:C levels of 20:80 (551.2 g/d, 10.7 kg/kg) and 50:50 (542.3 g/d, 10.6 kg/kg). It was obvious that FCR was best in the group fed on urea-treated rice straw (13.8 kg/kg) as compared to untreated rice straw fed-group (24 kg/kg). Carcass compositions of buffaloes measured resulted in 48.2 dressing percentage in all treatments offered at R:C levels of 50:50 and 20:80, however, loin eye area were 46.0, 53.6, 50.0 and $54.0cm^2$ for RS and UTS at respective levels of R:C. It was notable that carcass fat content was low which resulted in higher content of lean meat particularly in group fed UTS at 50:50 ratio R:C. Simple cost-net profit analysis was performed, it was found that net profits were obtained as follows 27, 30, -47, 44, 58, 22 $US/hd for respective treatment groups of RS and UTS at respective R:C levels. As shown, the best net profit resulted in group fed UTS at 50:50 R:C level.

Effect of Pre-partum Feeding of Crossbred Cows on Growth Performance, Metabolic Profile and Immune Status of Calves

  • Panigrahi, B.;Pandey, H.N.;Pattanaik, A.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.5
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    • pp.661-666
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    • 2005
  • The effects of pre-partum feeding management in terms of birth weight, growth, metabolic profile and immunity of calves were studied using 24 crossbred (Bos taurus${\times}$Bos indicus) cows, divided into three equal groups. The dietary treatments included feeding of either 3.0 kg concentrate/head/d throughout the 60 d pre-partum (T$_1$), or 3.0 kg concentrate during 60-22 d pre-partum and thereafter at an increased allowance at 0.25 kg/d during the next 21 d till it reached 1% of live weight (T$_2$). The third group of cows was fed similar to T$_2$, except that the concentrate feeding during 60-22 d pre-partum was reduced to 2.0 kg (T$_3$). All the groups had access to ad libitum green fodder throughout. The results revealed that the mean daily dry matter (DM) intake by the cows was similar (p>0.05) among the three groups during the 60 days of the pre-partum but T2 animals tended to gain more live weight (41.25 kg) than T$_1$ (38.12 kg) and T$_3$ (36.25 kg). The body condition score of the cows did not change appreciably over the experimental period. The mean birth weight of the calves was 24.00${\pm}$1.10, 24.63${\pm}$1.17 and 23.25${\pm}$1.19 kg for the three groups, respectively, with the corresponding average daily gain of 154.2, 155.0 and 169.7 g during the subsequent 60 days; both these parameters did not vary significantly ascribable to prepartum feeding regimens of their dams. The total immunoglobulin (Ig) concentration in the colostrum was 6.31${\pm}$0.34, 5.80${\pm}$0.21 and 6.13${\pm}$0.30 g/dl for the three groups, respectively, showing no influence of dietary treatments. The mean serum Ig levels (T$_1$ 2.10${\pm}$0.09, T$_2$ 2.05${\pm}$0.09 and T$_3$ 2.10${\pm}$0.12 g/dl) of calves at 5 d of age were similar among the dietary groups as was the case with various serum biochemical constituents. It is concluded that the variations in pre-partum dietary management elicited no significant influence on the calf performance including the immune status.

Protective Efficacy of Alpha-lipoic Acid against AflatoxinB1-induced Oxidative Damage in the Liver

  • Li, Y.;Ma, Q.G.;Zhao, L.H.;Guo, Y.Q.;Duan, G.X.;Zhang, J.Y.;Ji, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.6
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    • pp.907-915
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    • 2014
  • Alpha-lipoic acid (${\alpha}$-LA) is not only involved in energy metabolism, but is also a powerful antioxidant that can protect against hepatic oxidative stress induced by some drugs, toxins, or under various physiological and pathophysiological conditions. Here, we investigated the effect of ${\alpha}$-LA against liver oxidative damage in broilers exposed to aflatoxin $B_1$ ($AFB_1$). Birds were randomly divided into four groups and assigned different diets: basal diet, 300 mg/kg ${\alpha}$-LA supplementation in basal diet, diet containing 74 ${\mu}g/kg$ $AFB_1$, and 300 mg/kg ${\alpha}$-LA supplementation in diet containing 74 ${\mu}g/kg$ $AFB_1$, for 3 weeks. The results revealed that the addition of 300 mg/kg ${\alpha}$-LA protected against the liver function damage of broilers induced by chronic low dose of $AFB_1$ as estimated by a significant (p<0.05) change in levels of plasma total protein, albumin, alkaline phosphatase and the activities of liver glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase. The histopathological analysis also showed that liver tissues were injured in the $AFB_1$ diet, but this effect was alleviated by the addition of 300 mg/kg ${\alpha}$-LA. Additionally, $AFB_1$ induced a profound elevation of oxidative stress in birds, as indicated by an increase in malondialdehyde level, a decrease in glutathione peroxidase activity and a depletion of the glutathione content in the liver. All of these negative effects were inhibited by treatment with ${\alpha}$-LA. Our results suggest that the inhibition of $AFB_1$-induced excess production of lipid peroxides and the maintenance of intracellular antioxidant status may play important roles in the protective effects of ${\alpha}$-LA against $AFB_1$-induced oxidative damage in the liver.

CHARACTERISTICS OF HETEROEPITAXIALLY GROWN $Y_2$O$_3$ FILMS BY r-ICB FOR VLSI

  • Choi, S.C.;Cho, M.H.;Whangbo, S.W.;Kim, M.S.;Whang, C.N.;Kang, S.B.;Lee, S.I.;Lee, M.Y.
    • Journal of the Korean institute of surface engineering
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    • v.29 no.6
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    • pp.809-815
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    • 1996
  • $Y_2O_3$-based metal-insulator-semiconductor (MIS) structure on p-Si(100) has been studied. Films were prepared by UHV reactive ionized cluster beam deposition (r-ICBD) system. The base pressure of the system was about $1 \times 10^{-9}$ -9/ Torr and the process pressure $2 \times 10^{-5}$ Torr in oxygen ambience. Glancing X-ray diffraction(GXRD) and in-situ reflection high energy electron diffracton(RHEED) analyses were performed to investigate the crystallinity of the films. The results show phase change from amorphous state to crystalline one with increasingqr acceleration voltage and substrate temperature. It is also found that the phase transformation from $Y_2O_3$(111)//Si(100) to $Y_2O_3$(110)//Si(100) in growing directions takes place between $500^{\circ}C$ and $700^{\circ}C$. Especially as acceleration voltage is increased, preferentially oriented crystallinity was increased. Finally under the condition of above substrate temperature $700^{\circ}C$ and acceleration voltage 5kV, the $Y_2O_3$films are found to be grown epitaxially in direction of $Y_2O_3$(1l0)//Si(100) by observation of transmission electron microscope(TEM). Capacitance-voltage and current-voltage measurements were conducted to characterize Al/$Y_2O_3$/Si MIS structure with varying acceleration voltage and substrate temperature. Deposited $Y_2O_3$ films of thickness of nearly 300$\AA$ show that the breakdown field increases to 7~8MV /cm at the same conditon of epitaxial growing. These results also coincide with XPS spectra which indicate better stoichiometric characteristic in the condition of better crystalline one. After oxidation the breakdown field increases to 13MV /cm because the MIS structure contains interface silicon oxide of about 30$\AA$. In this case the dielectric constant of only $Y_2O_3$ layer is found to be $\in$15.6. These results have demonstrated the potential of using yttrium oxide for future VLSI/ULSI gate insulator applications.

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Change of Vegetation Characteristics and Soil Chemical Properties at Saemangeum Reclaimed Land in Korea (새만금 신간척지 식생과 토양화학성의 변화)

  • Kim, Sun;Jeong, Jae-Hyeok;Lee, Jang-Hee;Choi, Weon-Young;Lee, Kyeong-Bo;Im, Il-Bin
    • Weed & Turfgrass Science
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    • v.2 no.3
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    • pp.260-266
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    • 2013
  • This study was conducted to investigate changes of vegetation and soil characteristics to tidal land of Saemangeum reclaimed land from 2010 to 2012. Soil salinity was 0.16-22.3 dS $m^{-1}$ in the first survey, while the three years later, it was decreased to 0.12-4.22 dS $m^{-1}$. Vegetations were classified as 6 families and 26 species but it was increased to 8 families and 31 species after three years. Numbers of average species in survey site were increased from 7.1 species to 10.6 species. Numbers of vegetation were increased at each survey sites except for survey site 7 : there was decreased halophyte according to decrease in the soil salinity. Biomass production was increased at low production site, and showed highest production in area of dominant vegetation as Phragmites communis. Simpson's dominance ratio(SDR) of main vegetation as Phragmites communis, Calamagrostis epigeios were increased but Suaeda maritima, Salicornia europaea, Puccinellia nipponica and Zoysia sinica were decreased.