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Studies on the Stomatal Movement and Related Environmental Factors to Stomate in the Wheat I. Measurement of the Stomatal Aperture and Diurnal Movement of the Stomata in Wheat 1.Measurement of the Stonatal Aperture and Diurnal Movement of the Stomanta on Wheat (소맥엽신의 기공운동과 기공의 환경변이에 관한 연구 제1보 소맥엽신의 기공개도 측정법 및 기공개도의 일변화)

  • 남윤일;하용웅
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.27 no.2
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    • pp.130-136
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    • 1982
  • Experiments were conducted to establish a measuring method of the stomatal aperture in the leaves of the wheat plant, and to find the diurnal movements of the stomate in leaves of different position and tillers. The measurement methods used were the infiltration and the microrelief impression methods. The aperture of the stomate in the infiltration method was expressed in terms of the solutions pent ration into the leaf and this was refered to as the infiltration score. A score I represents injection with 10% iso-butyl alcohol+90% ethylene glycol solution and a score 7 represents injection with 70% iso-butyl alcohol +30% ethlene glycol solution. A linear relationship was obtained between the infiltration score and average pore width in a large number. of the stomata observed in the leaves of the adaxial and abaxial epidermis. The aperature of the stomate of flag and the 1st leaf were exhibited diurnal change with the maximum aperture at 10 A.M. but that of 2nd leaf reached maximum aperture 2 hours later than upper two leaves. After reaching the maximum aperture the stomata gradually closed and then completely closed at 6 P.M. The aperture of the stomate in the adaxial epidermis and the base part of the leaf were larger than those in the abaxial and top part of the leaf, and aperture of the stomate in the leaves of the main stem was larger than those on the tillers.

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Studies on the EPA(eicosapentaenioc acid) production by marine bacteria (해양세균(海洋細菌)을 이용(利用)한 EPA(eicosapentaenoic acid) 생성(生成)에 관한 연구(硏究))

  • Joh, Yong-Goe;Kim, Seong-Jin;Kim, Ji-Soo;Lee, Min-Kyeng
    • Journal of the Korean Applied Science and Technology
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    • v.12 no.2
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    • pp.121-130
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    • 1995
  • This project has been worked out for isolation of EPA-producing bacteria from marine source of sea water, sea sediment and intestinal contents eviscerated from some red-muscle fish such as mackerel, horse-mackerel and spike fish. The samples were precultured on the media of PPES-II glucose broth and then pure-cultured on Nutrient agar and P-Y-M glucose. Lipids extracted from those bacterial mass collected by centrifugation were analysed in terms of lipid class and fatty acid composition. The results are resumed as follows : 1. 112 strains from sea water and 76 strains from sea sediment were tested for their EPA producing capability, but both strains of (SA-67 and SA-91) from the former and four strains(SS-35, 37, 51 and 71) from the latter have been proved to produce EPA above the level of 2% of total fatty acids. The strains such as GS-11, 29, 31, HM-9, 29, B-18, 33, 107, YL-129, 156, 203, 77, 104 and 256 which were isolated from fish intestinal contents, have also produced EPA at higher level than 2% of total fatty acids. 2. Contents of total lipids extracted from the cultures of these strains grown at $25^{\circ}C$, range from 2.8% to 6.9% (on dry weight %), and they are mainly composed of polar lipids($40.9{\sim}52.9%$) such as phosphatidyl glycerol($^{+}cardiolipin$)(?) and phosphatidyl ethanolamine ($33.8{\sim}40.0%$), with smaller amount of free fatty acid ($11.2{\sim}20.2%$). 3. EPA was isolated from a mixture of fatty acid methyl esters obtained from the lipid of each strain by HPLC in silver-ion mode and was identified by GC-Mass spectrometry. 4. The strains of SW-91, GS-11, GS-29, HM-9, B-18 and YL-203 grown at $25^{\circ}C$ have a level of 5% EPA in their total fatty acids, and the GS-11 and HM-9 strains show a tendency of increase in the EPA level with an increase of growth temperature.

Heavy Metal Contents of Marketing Salts and Bay Salts by Heating (시판 소금의 중금속 함량과 천일염의 온도변화에 따른 중금속 함량)

  • 홍광택;이종영;장봉기
    • Journal of environmental and Sanitary engineering
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    • v.11 no.3
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    • pp.79-84
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    • 1996
  • In order to determine the content of heavy metal in common salts, 35 bay salt samples, 7 refined salt samples, 7 fine salt samples, 5 bake salt samples and 5 bamboo bake salt samples were collected from old market in major cities. Heating bay salt 35 samples, it made heating bay salt of $500^{\circ}C$, /TEX>(34 samples), heating bay salt of $1000^{\circ}C$(35 samples). These were analysed for contents of lead(Pb), cadmium(Cd), iron(Fe) and zinc(Zn) by atomic absorption spectrophotometer. The results were as follows : Mean content of lead in bay salts was $0.124{\pm}0.035ppm$ refined salt was $0.130{\pm}0.019ppm$, fine salt was $0.073{\pm}0.036ppm$ bake salt was $0.097{\pm}0.023ppm$, bamboo bake salt was $0.117{\pm}0.020ppm$, heating bay salt was $0.063{\pm} 0.021ppm$ in $500^{\circ}C$, heating bay salt was $0.063{\pm}0.039ppm$ in $1000^{\circ}C$. And bay salt refined salt bamboo bake salt were not significant one another. Mean content of cadmium in bay salts was $0.031{\pm}0.008ppm$ refined salt was $0.032{\pm}0.003ppm$, fine salt was $0.037{\pm}0.005ppm$, bake salt was $0.169{\pm}0.117ppm$ bamboo bake salt was $0.079{\pm}0.052ppm$, heating bay salt of $500^{\circ}C$ was $0.030{\pm}0.029ppm$ heating bay salt of $1000^{\circ}C$ was $0.017{\pm}0.013ppm$. And bay salt refined salt, fine salt were not significant one another. Mean content of iron in bay salts was $1.025{\pm}0, 634ppm$, refined salt was $0.359{\pm}0.163ppm$ fine salt was $0.267{\pm}0.068ppm$, bake salt was $2.929{\pm}1.963ppm$, bamboo bake salt was $5.378{\pm}3.676ppm$, heating bay salt of $500^{\circ}C$ was $0.847{\pm}0.315ppm$ heating bay salt of $1000^{\circ}C$ was $0.991{\pm}0.868ppm$. And bay salt refined salt, fine salt, bake salt, bamboo bake salt were significant one another(p<0, 01). Mean content of zinc in bay salts was $0.253{\pm}0.154ppm$, refined salt was $0.263{\pm}0.091ppm$ fine salt was $0.187{\pm}0.015ppm$, bake salt was $0.166{\pm}0.034ppm$, bamboo bake salt was $0.282{\pm}0.064ppm$, heating bay salt of $500^{\circ}C$ was $0.253{\pm}0.085ppm$, heating bay salt of $1000^{\circ} C$ was $0.242{\pm}0.179ppm$. And bay salt refined salt fine salt, bake salt, bamboo bake salt were not significant one another.

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Infection status with trematode metacercariae in the fresh-water fish from Chunamchosuchi (pond), Uichang-gun, Kyongsangnam-do, Korea (경남 의창군 주남저수지산 담수어의 흡충류 피낭유충 감염상)

  • 손운목;최연순
    • Parasites, Hosts and Diseases
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    • v.35 no.3
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    • pp.165-170
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    • 1997
  • The present study was performed to analyze the infection status of trematode metacercariae in fishes caught from Chunamchosuchi (pond) located in Uichang-gun. Kyongsangnam-do. A total of 130 freshwater fish of 5 species was collected by a fish net and fish traps from November, 1995 to May, 1996. They were examined under a stereomicroscope after artificial digestion with pepsin-HCI solution. A total of 8 species or metacercaria, i. e. Clonorchis sinensis, Echinochnsnw japonicas, Cvathocotwle orientalis, Diplostomun sp.. Metorchis orientalis. Holostephcnw nipponicw, Exorchis oviformis and unidentified echinostome, was detected from them. The metacercariae of C. sinenesis were found in 8/20 (40.0%) Accnthorhodeus Qsmwsi, 20/20 (100%) Cutter breuiccudc, 31/45 (68.9) Cultriculus eigenmanni and 21/25 (84.0%) Pseunorasborc pronga, and the average number of metacercariae detected in each fish species were 1.9, 31.7. I5.3, and 73.0. From the above results, it was confirmed that fresh-water fishes from Chunamchosuchi (pond) were highly infected with metacercariae of avian trematode, i.e. C. orientolis, H. nipponicus. M. oui,entnlis, E. jcponicw and Diplostonum sp., and 4 species of fish. P. parvc, C. breuiccudn, C. eigenmnnni and A. asmussi, were infected with metacaecariae of C. sinensis.

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Drying of Crops with Solar Heated Air -Drying of Rough Rice - (태양열을 이용한 농산물건조에 관한 연구 (I)-벼의 건조에 대하여)

  • 이문남;금동혁;류능환
    • Journal of Biosystems Engineering
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    • v.3 no.2
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    • pp.100-113
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    • 1978
  • Drying grain with conventional artificial drying methods requires great quantities of petroleum fuels. Depletion of fossil fuel increases the need of the utilization of solar energy as an alternative to petroluem fuels for drying grain , an energy intensive agricultural operation. Many techniques for the utilization of solar energy in grain drying have been developed, however, there are many problems in adopting solar energy as an energy sources for drying grain. Futhermore, very little research has been done on solar grain drying in Korea. This study was conducted to evaluate the availability of solar energy for drying of rough rice in Chuncheon, Suweon, and Jinju areas based on 50year meteorological data, and to analyze experimentally the performance of a solar air collector for dying grain, and to find the effects of solar heated air compared to unheated air on the rate of drying and energy consumption required for drying of rough rice. The results of this study was may be summarized as follows ; 1. Monthly average daily total radiation on a horizontal surface in October was 260.6 ly/day for Chuncheon, 240.3 ly/day for Suweon , and 253.4 ly/day for Jinju area, respectively. 2. the ratio of monthly average daily diffuse radiation to daily total radiation on a horizontal surface was approximately 0.41 for Chuncheon, 0.45 for Suweon, and 0.44 for Jinju area, respectively. 3. Although the statistical distribution curves of daily total radiation for the three locations were not identical , the differences among them were not large and may be neglected for many practical purposes. 4. I was estimated that the optimum tilting angle of the collector in October was approximately 46 degrees for Chuncheon and Suweon and 45 degrees for Jinju. 5. The ratio of the total radiation on a optimum tilting plane to that on a horizontal plane was estimated to be 1.36 for Chuncheon, 1.31 for Suweon, and 1.27 for Jinju , respectively. 6. The collection efficiency of the solar air collector ranged from 47. 8 to 51. 5 percent at the air flow rates of 251. 1-372.96 $m^3$/hr. High efficiency remained nearly , constant during the best sunshine hours, 10 a.m. to 2 p.m. and decreased during other hours. More energy was collected as the air flow rate incresed. 7. The average temperature rise in the drying air from the solar collector for the test period varied from $6.5^\circC$ to $21.8^\circC$ above the ambient air temperature. 8. Solar-dried rough rice averaged 13.7 percent moisture (w.b.) after 130 hours of drying with the air flow rate of 1. 64 ccm/$m^3$, and rough rice dried with natural air averaged 15.1 percent moisture (w.b.) after 325 hours of drying with the same air flow rate. 9. Energy saving of 2.4 kwh per $m^3$ percentage point of moisture removed was obtained from solar heated air drYing. The solar bin used 53.3 percent less energy per percentage point of moisture removed than the natural air bin.

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A 3-D Vision Sensor Implementation on Multiple DSPs TMS320C31 (다중 TMS320C31 DSP를 사용한 3-D 비젼센서 Implementation)

  • Oksenhendler, V.;Bensrhair, Abdelaziz;Miche, Pierre;Lee, Sang-Goog
    • Journal of Sensor Science and Technology
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    • v.7 no.2
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    • pp.124-130
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    • 1998
  • High-speed 3D vision systems are essential for autonomous robot or vehicle control applications. In our study, a stereo vision process has been developed. It consists of three steps : extraction of edges in right and left images, matching corresponding edges and calculation of the 3D map. This process is implemented in a VME 150/40 Imaging Technology vision system. It is a modular system composed by a display, an acquisition, a four Mbytes image frame memory, and three computational cards. Programmable accelerator computational modules are running at 40 MHz and are based on TMS320C31 DSP with a $64{\times}32$ bit instruction cache and two $1024{\times}32$ bit internal RAMs. Each is equipped with 512 Kbytes static RAM, 4 Mbytes image memory, 1 Mbytes flash EEPROM and a serial port. Data transfers and communications between modules are provided by three 8 bit global video bus, and three local configurable pipeline 8 bit video bus. The VME bus is dedicated to system management. Tasks between DSPs are distributed as follows: two DSPs are used to edges detection, one for the right image and the other for the left one. The last processor computes the matching process and the 3D calculation. With $512{\times}512$ pixels images, this sensor generates dense 3D maps at a rate of about 1 Hz depending of the scene complexity. Results can surely be improved by using a special suited multiprocessors cards.

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THE EFFECT OF DIFFERENTIAL MODULATION OF N-METHYL-D-ASPARTATE RECEPTOR ON THE PROLIFERATION OF PRIMARY CULTURED NORMAL HUMAN ORAL KERATINOCYTES: DNA SYNTHESIS RATE ANALYSIS (N-methyl-D-aspartate 수용기의 다양한 조절이 일차 배양된 정상사람구강각화세포의 증식에 미치는 영향; DNA 합성율 평가)

  • Kim, In-Soo;Paik, Ki-Suk;Chang, Mi-Sook;Lee, Won;Lee, Seung-Pyo
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.33 no.2
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    • pp.124-130
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    • 2007
  • In the present study, I investigated the effects of N-methyl-D-aspartate (NMDA), arachidonic acid (AA), and Nitric Oxide Synthase Inhibitor (NOSI), alone or in combination, on the proliferation of cultured primary normal human oral keratinocytes (NHOK). The purpose of this study was therefore the preliminary study for the examination of the interaction between these agents and NHOK in order to elucidate the mechanisms by which epithelial growth and regeneration are regulated. NHOK were obtained from gingival tissue of 20 individuals aged 20 to 29, and third passage (P3) cells were used for this study. The DNA synthesis was measured by the BrdU assay. Addition of low concentration of AA ($1{\mu}M$) and high concentration of AA with NMDA group (NMDA+AA $10{\mu}M$) made DNA synthesis rate increase significantly at the early stage. Adding NNA ($10{\mu}M$) affected DNA synthesis rate to increase significantly in 4 hours. At the early stage, DNA synthesis was significantly active in the NOS-I with NMDA groups than in the control and the NMDA-only group, while it didn't become statistically meaningful in 24 hours. AA $1{\mu}M$ and NNA $10{\mu}M$ may induce the proliferation of the NHOK independently and NOS-I may induce the proliferation of the NHOK with NMDA. These reactions might be related to the NMDA receptor in the cell and the change of the intracellular calcium ion concentration.

A Study for the stability of chopped garlic quality (다진 마늘의 품질안정을 위한 연구)

  • 나영아
    • Culinary science and hospitality research
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    • v.5 no.2
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    • pp.115-130
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    • 1999
  • Preservative effects of natural preservatives, citric acid and salt on chopped garlic were investigated. Citric acid was very effective for the repression of bacteria multiplying and browning of chopped garlic in 0.5%~1% concentration. Salt had an effect on the repression of bacteria multiplying and browning color of chopped garlic except for 1% NaCl. Synergy effect between citric acid and NaCl was also very good for the decreasing of bacteria multiplying and the maintaining of Hunter color of chopped garlic. Compounded effect among the GF, CA and ascorbic acid was somewhat proper in the sensory evaluation of chopped garlic. And the sensory evaluation score was best in chopping size 3mm(diameter) of chopped garlic and viscosity 4500cp. of chopped garlic.

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Comparison of Yield and Content of Salidroside with Application Rates of Nitrogenic Fertilizer under Forcing Culture of Rhodiola rosea L. (바위돌꽃(Rhodiola rosea L.)의 촉성재배시 질소 시비에 따른 수량과 Salidroside 함량)

  • Lee, Kang-Soo;Choi, Sun-Yeong;Li, Long-Gen;Hwang, Seon-Ah
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.2
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    • pp.124-130
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    • 2008
  • This experiment was conducted to investigate the optimum nitrogen fertilization conditions for the production of high-quality Rhodiola rosea L. in forcing culture. Up until 48 kg N/10 a of both urea and ammonium sulfate, dry matter yield of root of Rhodiola rosea L. tended to increase with increase in application rates, however, it decreased thereafter in higher application rates. In the case of urea, the content of salidroside in the root of the Rhodiola rosea L. appeared to decrease rapidly from the application rates of 64 kg N/10 a and over. Meanwhile, the content of salidroside in the root tended to decrease gradually with the application rates exceeding 64 kg N/10 a of ammonium sulfate. The optimum fertilization rates of urea and ammonium sulfate was 45-8-20-10-10 (N-P-K-Ca-Mg) kg/10 a according to the curvilinear regression equation. However, considering the nitrogen accumulation in soil, nitrogen translocation into the plant, and dry matter yield and content of salidroside in the root of Rhodiola rosea L., the optimum fertilization rates of urea and ammonium sulfate would be 40-8-20-10-10 kg/10 a and 35-8-20-10-10 kg/10 a, respectively.

ANALYSIS OF TEMPERATURE RISE ON THE ROOT SURFACE DURING CONTINUOUS WAVE OF CONDENSATION TECHNIQUE (Continuous Wave of Condensation Technique으로 근관충전시 치근면 온도상승 분석)

  • Kim, Young-Ju;Hwang, Yun-Chan;Kim, Sun-Ho;Hwang, In-Nam;Choi, Bo-Young;Jeong, Young-Jin;Juhng, Woo-Nam;Oh, Won-Mann
    • Restorative Dentistry and Endodontics
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    • v.28 no.4
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    • pp.341-347
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    • 2003
  • This study was conducted to evaluate the temperature rise on the root surface while the root canal is being obturated using continuous wave of condensation technique. Maxillary central incisor was prepared for repeated canal obturation. Ten thermocouples (Omega Engineering Inc., Stanford, USA) were placed at 1 mm increment from the anatomical root apex. The real temperature of Buchanan plugger was recorded before insertion into the root canal. The root canal was obturated with continuous wave of condensation technique as described by Buchanan and the root surface temperature was recorded during obturation at $150^{\circ}C,{\;}200^{\circ}C,{\;}250^{\circ}C{\;}and{\;}300^{\circ}C$ temperature settings of System B HeatSource (Model 1005, Analytic technologies, Redmond, WA, USA). After completion of the temperature recording, the dentinal-cementum thickness at each sites was measured. The data were analyzed using one-way ANOVA followed by Scheffe's test and linear regression test. The results were as follows. 1. When the temperature was set at $150^{\circ}C,{\;}200^{\circ}C,{\;}250^{\circ}C{\;}and{\;}300^{\circ}C$ on the digital display of System B HeatSource, the real temperature of the plugger at the 1mm point from the tip revealed $130.82{\pm}2.96^{\circ}C,{\;}158.00{\pm}5.26^{\circ}C,{\;}215.92{\pm}6.91^{\circ}C{\;}and{\;}249.88{\pm}3.65^{\circ}C$ respectively. 2. The position of 8 mm from the anatomical apex showed the highest temperature increase at each temperature settings and it was significantly higher than those of other positions (p<0.0l). The temperature rise was constantly increased toward coronal portion from apex of the root. 3. The maximum temperature increase on the root surface was $2.37{\pm}0.09^{\circ}C{\;}at{\;}150^{\circ}C{\;}setting,{\;}3.11{\pm}0.12^{\circ}C{\;}at{\;}200^{\circ}{\;}setting,{\;}3.93{\pm}0.09^{\circ}C{\;}at{\;}250^{\circ}C{\;}setting{\;}and{\;}5.69{\pm}0.15^{\circ}C{\;}at{\;}300^{\circ}C$ setting respectively. These results suggest that it be relatively kind to the supporting tissues of the root that the root canal is obturated using continuous wave of condensation technique at $150^{\circ}C,{\;}200^{\circ}C,{\;}250^{\circ}C{\;}and{\;}300^{\circ}C$ temperature settings on digital temperature display of System B HeatSource.