• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.2
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• pp.124-130
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• 1986

To find out the optimum split application ratios of fertilizer nitrogen and potassium in the newly opened pasture at spring, a field experiment was conducted. Two levels of nitrogen and potassium application and three split application ratios of both fertilizer were treated by split design with 4 replications. The experiment was carried out from March, 1984 to October, 1985 on the experimental field of the Livestock Experiment Station in Suweon. The results obtained are summarized as follow: 1. Total DM yield was not showed signigicant difference among split application ratios of nitrogen and potassium. 2. DM yield of grasses was 4,854 kg/ha in heavy basal application of N and $K_2$O (160 kgN: 140 kg $K_2$O/ha), it was significantly increased by 16% than of conventional dressing (80 kg N: 70 kg $K_2$O/ha). But there was almost no difference in dry matter among N and $K_2$O split application ratios. 3. Dry matter yield of legume in heavy basal dressing was decreased by 23% than that of conventional dressing. In the methods of NK application, dry matter yield of legume was tended to decrease by 3% with equal application and 9% with heavy dressing in autumn, when compared with that of heavy dressing in spring. 4. There was no significant difference in dry matter yield of native species between different basic fertilization levels, but dry matter yield of native species was decreased by 6% with equal application and more increased by 11% with heavy dressing in autumn, when compared with the yield of heavy dressing in spring. 5. In aspect of the botanical composition, the percentage distribution of grasses was increased and the ratio of legume was greatly decreased by heavy dressing at basal fertilization. Also for good maintenance of botanical composition, it is suggested that the equal split application of N and $K_2$O was desirable.

• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.207-214
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• 1996

To examine the developmental capacity of manipulated embryos after ultrarapid refreezing and thawing, mouse embryos were biopsied at 4-cell stage, frozen twice at 4-cell and morula stages, respectively, and then transferred to rec-ipients. Single blastomeres were biopsied from 4-cell embryos by a modified aspiration method. Biopsied 4-cell embryos were equilibrated into freezing medium at room temperature for 2.5 min, loaded into 40 $\mu$I of freezing medium in 0.25 ml plastic straw and then directly immersed into liqiud nitrogen. Freezing medium for 4-cell embryos consisted of 4.0 M ethylene glycol and O.25 M sucrose in dPBS supplemented with 6 mg/lm BSA. Morulae were frozen into freezing medium containing 5.0 M glycerol instead of ethylene glycol. Thawing was conducted by agitating each straw in 3TC water for 20 sec. The c content of each straw was expelled into 0.5 ml of dilution medium, which consisted of 0.25 M sucrose and 3 mg/ml BSA in dPBS. The thawed embryos were rehydrated in dilution medium for 10 min, washed 3 times with dPBS and then cultured in M16 medium at 37$^{\circ}C$, 5% CO$_2$ in air. Blastocysts that developed from frozen or refrozne biopsied embryos were transferred to recipients on Day 3 of pseudopregnancy, respectively. In vitro and in vivo developmental rates of the biopsied and intact 4~cell embryos after freezing and thawing were 78 (10l/130) and 25% (10/40), and 91 (114/125) and 30% (12/40), respectively. Although the rates of in vitro development of biopsied and intact embryos to blastocyst stage were significantly different after freezing and thawing (P

• Food Science and Preservation
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• v.19 no.1
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• pp.123-130
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• 2012

In this study, 80% ethanolic extracts of tartary and common buckwheats were assessed for their total phenol content, total flavonoids content, antioxidant activity (DPPH, ABTS radical scavenging activity and reducing power), and anti-adipogenic effects. Our results show that total phenol contents of 80% ethanolic extract from tartary and common buckwheats were $17.35{\pm}0.41$ and $8.20{\pm}0.28\;{\mu}g$ GAE/g, respectively. Antioxidant activities of 80% ethanolic extract from tartary buckwheat were significantly higher than that of common buckwheat extract (p<0.05). During adipocyte differentiation, 80% ethanolic extracts of tartary and common buckwheat significantly inhibited lipid accumulation compared to control cells. We further evaluated the effect of buckwheat extracts on the changes of key gene expression associated with 3T3-L1 adipogenesis and ROS production. Tartary buckwheat extract was more suppressed the mRNA expressions ($PPAR{\gamma}$ and aP2) than that of common buckwheat extract. Moreover, tartary buckwheat inhibited the mRNA expression of both NOX4 (NADPH oxidase 4) and G6PDH (glucose-6-phosphate dehydrogenase). These results indicate that anti-adipogenesis effect of tartary buckwheat can be attributed to phenolic compound that may potentially inhibit ROS production.

• Horticultural Science & Technology
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• v.29 no.2
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• pp.130-134
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• 2011

Paprika (Capsicum annuum L.), a colored bell-type sweet pepper, is one of the most important money making vegetable crops in Korea. The cultivation area, total production, and exports of paprika are gradually getting increased, but the paprika cultivars used in Korea are all imported. It was well-known that the genic male sterility (GMS) is the main way to produce paprika hybrid seeds. However, it is little known that how many and what kinds of ms genes are used for breeding of paprika $F_1$ varieties. In this study, eight paprika cultivars ('Special', 'Debla', 'Plenty', 'Fiero', 'Boogie', 'Fiesta', 'Derby', and 'Minibell'), popularly cultivated in Korea and three different genic male sterile lines ('GMSP', 'GMS3', and 'GMSK') were used. For allelism test among the $F_1$ cultivars, half diallel crosses were performed. The result demonstrated that the most of the GMS in paprika cultivars except for 'Minibell' were same allele. To identify which GMS gene(s) were used for paprika $F_1$ cultivars, top crosses between previously known GMS lines and the $F_1$ cultivars were performed. As a result, we found that the $ms_k$ and the $ms_p$ genes were alleles for the GMS of 'Minibell' and for the other cultivars, respectively. We also confirmed that the GMS gene identification using GMSK-CAPS marker linked to the $ms_k$ gene and the PmsM1-CAPS marker linked to the $ms_p$ gene in $F_2$ progenies of 'Minibell' and 'Fiesta' and 'Derby' cultivars, respectively. In addition, we developed the PmsM2-CAPS marker for 'Plenty', 'Fiero', and 'Boogie' cultivars. We expect that these markers will be very useful for breeding new maternal (male sterile) line of paprika.

• Journal of Periodontal and Implant Science
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• v.30 no.4
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• pp.835-852
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• 2000

The ultimate goal of periodontal therapy is the regeneration of periodontal tissue and repair of function. For more than a decade there have been many efforts to develop materials and methods of treatment to promote periodontal wound healing. Recently many efforts are concentrated on the regeneration potential of material used in oriental medicine. In some in vitro and in vivo experiments, there have been many evidences that these materials have an effect on bone regeneration. The purpose of this study was to evaluate histologically and radiologically in Sprague-Dawley rats the effects of safflower seed extracts on the regeneration of the calvarial defects surgically produced. So in this study, the critical size defects were surgically produced in the calvarial bone of 30 Sprague-Dawley rats using the 8mm trephine bur. The safflower seed extract was applied into the defect of each rat in experimental group, whereas nothing was applied into the defect of each rat in control group. Rats were sacrificed at 2, 4, 8 weeks following operation and histomorphometric and radiodensitometric analysis were performed. 1. The newly formed bone length was $102.91{\pm}22.05$, $178.29{\pm}24.40$ at 2 week in the each control, experimental group, $130.95{\pm}39.24$, $242.62{\pm}50.33$ at 4 week and $181.53{\pm}76.35$, $240.36{\pm}22.00$ at 8 week($unit,{\mu}m$). In the 2, 4 week, there were statistically significant difference between control and experimental group(P<0.05). 2. The newly formed bone area was $2962.06{\pm}1284.48$, $10648.35{\pm}1284.48$ at 2 week, $5103.25{\pm}1375.88$, $9706.78{\pm}1481.81$ at 4 week, $8046.02{\pm}818.99$, $12057.06{\pm}740.47$ at 8 week($unit,{\mu}m^2$). In every week, there were statistically significant difference between control and experimental group(P<0.05). 3. The radiopacity was $14.26{\pm}.33$, $25.47{\pm}4.33$ at 2 week, $20.06{\pm}9.07$, $26.61{\pm}2.78$ at 4 week, $22.99{\pm}3.76$, $27.29{\pm}1.54$ at 8 week(unit, %). In the 2 week, there was statistically significant difference between control and experimental group(P<0.05). In conclusion, the results of the present study suggest that safflower seed extract initially has an effect on the newly formed bone area, length and radiopacity when it is applied to the calvarial defect of Sprague - Dawley rat. Then. the material has an effect on newly formed bone area and length.

• The Korean Journal of Malacology
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• v.20 no.2
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• pp.125-130
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• 2004

Arkshell, Scapharca broughtonii spats were placed in natural environmental condition for 30 days from July 28 to August 27, 2004, in order to estabilish intermediate culture technique. Growth and survival of the spats in different intermediate culture areas with various culture methods were measured. Water temperature of studied area ranged from 24.1 to 28.5$^{\circ}C$, salinity was 15.4 to 33.3 psu, dissolved oxygen was 3.92 to 12.6 mg/l. Scapharca broughtonii spats cultured in Yeosu developed the best, 10.15 ${\pm}$ 1.12 mm in average shell length, and the highest survival was recorded as 77% in Namhae. Shell lengths of the Scapharca broughtonii spats cultured in the water depths of 2, 5, and 10 m were 7.14 ${\pm}$ 1.14 mm, 6.98 ${\pm}$ 1.74 mm and 6.27 ${\pm}$ 1.33 mm, and the survivals showed 75.5%, 77.0% and 76.5%, respectively. When 1 mm, 2 mm, and 3 mm-sized spats were cultured for 30 days in water depth of 5 m, the shell length increased to 6.73 ${\pm}$ 1.46 mm, 6.98 ${\pm}$ 1.74 mm and 7.04 ${\pm}$ 1.19 mm, and survivals were 67.0%, 77.0% and 58.5%, respectively. The shell lengths of spat cultured for 30 days in mesh sizes of 1 ${\times}$ 1 mm, 2 ${\times}$ 2 mm and 3 ${\times}$ 3 mm, 5 m below the surface were 8.14 ${\pm}$1.23 mm, 8.26 ${\pm}$ 1.19 mm and 8.78 ${\pm}$ 1.16 mm, and survivals were 41.5%, 43.0% and 44.5%, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.2
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• pp.130-136
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• 1991

This study was carried out under the appropriate conditions of moisture content(60~70%), C/N ratio(25~30), and C/P ratio(30~35) on the mixed materials of cow dung and rice hulls. The good mixing ratio of cow dung and rice hulls was woth to one on volume basis which was able to adjust to the moisture content and C/N ratio of the mixing material. During fermentation pile period, the mixing ratio of control, non-aerated pile with turning was two to one and that of aerated pile with blower was two ti one. In fermentation pile period, average temperature of aerated pile was $55{\sim}65^{\circ}C$ and it was more higher than control of $40{\sim}50^{\circ}C$ non-aerated pile. While total nitrogen content of aerated pile was lower than that of the control, total carbon content and C/N ratio were higher. In cure pile period, the temperature and C/N ratio of aerated pile were increased at the early stage and then they were decreased, but total nitrogen and total carbon contents of aerated pile were increased in process of days. Final product in aerated pile had more fine particles and was good for the growth of cucumber seedlings than control.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.5
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• pp.624-630
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• 2001

Two experiments on the effects of molasses additive on cassava tops silage quality to its feed intake and digestibility by growing Holstein$\times$local crossbred heifers were carried out. Sixteen plastic bags of one meter diameter and two meters length were allocated in a $2{\times}2$ factorial design with four replicates in the ensiling study, with and without the molasses additive and with two storage times (2 and 3 months). The silage produced in the first experiment was used in the feed intake and digestibility study. Six crossbred Holstein heifers, 160-180 kg live weight, were randomly allocated in a $3{\times}2$ change-over design to three treatments: Guinea grass ad libitum, 70% of grass ad libitum with a supplement of non-molasses cassava silage ad libitum, and 70% of grass ad libitum with a supplement of molasses cassava silage ad libitum. Ensiling was shown to be a satisfactory method for preservation of cassava tops. The HCN content was significantly reduced from $840mg\;kg^{-1}$ to 300 or $130mg\;kg^{-1}$, depending on storage period. The tannin content was not significantly changed. Molasses additive resulted in lower pH, Crude Protein (CP), NDF and higher DM content but did not otherwise affect chemical composition. The voluntary feed intake per 100 kg live weight of the heifers was 2.59, 2.65 and 2.91 kg DM of Guinea grass, non-molasses cassava tops silage and molasses cassava tops silage diet, respectively. Crude protein intake was significantly improved in the cassava tops silage diets. The apparent digestibility of DM, OM, CP, NDF and ADF decreased with the silage supplement diets. No significant difference in digestibility was found between the non-molasses and molasses silage diets. The digestibility coefficient of DM, OM, CP, NDF, ADF in non-molasses cassava tops silage and molasses cassava tops silage was 49.4, 52.1, 45.81, 36.6, 27.7 and 49.7, 51.9, 47.55, 28.1, 19.5, respectively. It is concluded that cassava tops can be preserved successfully by ensiling and that cassava tops silage is a good feed resource for cattle.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.8
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• pp.1018-1025
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• 2017

The present study collected 18 kinds of commercially circulated liquid Sansuyu (Corni fructus) in pouch form and conducted comparative analysis on markings and physicochemical characteristics in order to utilize this information as a base material for the development of Sansuyu liquid products. The most abundant valid substance was morroniside, followed by loganin and cornin, in that order. Total concentration of valid substances in Cornus fruit products produced in the Gurye region was 168.50 mg/100 g, which was significantly higher than the overall average of 130.89 mg/100 g. There were seven (1st) and seven (2nd) kinds that contained more morroniside than the overall average, six (1st) and four (2nd) kinds with more loganin than the overall average, and eight (1st) and one (2nd) kinds with more cornin than the overall average. There were also five types in which no loganin was detected. There were significant differences in the valid substances of most products according to production period. Overall, these results show that processing method standardization research on extraction conditions is needed to produce liquid Sansuyu products of excellent quality.

• Journal of Radiation Protection and Research
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• v.28 no.3
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• pp.233-237
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• 2003

It has been known that ATM plays a central role in response of cells to ionizing radiation by enhancing DNA repair. We have investigated the feasibility of increasing radiosensitivity of tumor cells with the use of ATM inhibitors such as caffeine, pentoxifylline and wortmannin. Human colorectal cancer RKO.C cells and RKO-ATM cells (RKO cells overexpressing ATM) were used in the present study. The clonogenic cell survival in vitro indicated that RKO-ATM cells were markdely radioresistant than RKO.C cells. Treatment with 3 mM of caffeine significantly increased the radiosensitivity of cells, particulary the RKO-ATM cells, so that the radiosensitivity of RKO.C cells and RKO-ATM cells were almost similar. The radiation induced G2/M arrest in RKO-ATM cells was noticeably longer than that in RKO.C cells and caffeine treatment significantly reduced the length of the radiation induced G2/M arrest in both RKO.C and RKO-ATM cells. Pentoxifylline and wortmannin were also less effective than caffeine to radiosensitize RKO.C or RKO-ATM cells. However, wortmannin was more effective than caffeine against human lung adenocarcinoma A549 cells indicating the efficacy of ATM inhibitor to increase radiosensitivity is cell line dependent. For in vivo study, RKO.C cells were injected s.c. into the hind-leg of BALB/C-nuslc nude mice, and allowed to grow to 130mm3 tumor. The mice were i.p. injected with caffeine solution or saline and the tumors irradiated with 10 Gy of X-rays. The radiation induced growth delay was markedly increased by 1-2 mg/g of caffeine. It was concluded that caffeine increases radiosensitivity of tumor cells by inhibiting ATM kinase function, thereby inhibiting DNA repair, that occurs during the G2/M arrest after radiation.