• BMB Reports
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• v.32 no.5
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• pp.436-439
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• 1999

Shigella sonnei is an important cause of human enteric infections. S. sonnei KNIH104S was previously reported to be isolated from Korean shigellosis patients. We cloned a 2.8-kb KpnI fragment containing the recA gene encoding a recombinase from the chromosomal DNA of S. sonnei KNIH104S. This recombinant plasmid was named pRAK28. E. coli HB101, a recA mutant, cannot grow on Luria-Bertani medium in the presence of the alkylating agent methylmethane sulfonate, however, E. coli HB101 harboring pRAK28 was found to grow on this medium. As far as we know, we are the first to sequence the recA gene from S. sonnei. This gene is composed of 1062 base pairs with an ATG initiation codon and a TAA termination codon. Nucleotide sequence comparison of the S. sonnei recA gene exhibited 99.7% and 99.5% identity with those of S. flexneri and E. coli, respectively.

• Food Science of Animal Resources
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• v.24 no.2
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• pp.108-114
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• 2004

In a trial involving 72 pigs, the effects of market weight and gender on the carcass and pork quality characteristics were investigated. A total of 72 pigs were divided into 3 groups(95-104, 105-110 or 111-120kg), market weight was assigned to 2 gender group (gilt or boar). The carcass characteristics (carcass weight, backfat thickness or grades) were determined on those carcass, longissimus muscle was removed from each left side at 5th to 13th rib and meat qualities were evaluated. The carcass weight and backfat thickness of pigs slaughtered at 111-120kg were increases than the other weights. The carcass grade of pigs slaughtered at 105-110kg had higher then at pigs slaughtered 94-104kg. Compared with boars, gilts carcass had higher in carcass weight and backfat fat. The pH$\_$u/, drip loss and cooking loss tended to similar for market weight and gender, meat of boars had higher shear force values than gilts (p＜0.05). The meat color tended to similar for market weight and gender. The total myoglobin content of gilts slaughtered at 95-104kg and boars slaughtered at 111-120kg had higher than the other weight and gender. The meat of gilts had higher springiness and brittleness than boars (p＜0.05). These results imply that the carcass characteristics (carcass weight and backfat thickness) could be affected by market weight and gender, meat of gilts was improved the shear force values and texture properties when compared to boars.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.585-591
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• 2001

To compare the p10 promoter activity of Bombyx mori nucleopolyhedrovirus (BmNPV)K1 and K4, recombinant viruses Bm101-LacZ and Bm104-LacZ with a lacZ gene under the control of each p10 promoter were constructed. The $\beta$-galactosidase activity due to Bm101-LacZ was about 5.5- and 1.1-fold higher than that due to Bm104-LacZ and BmK1-LacZ, respectively. expressing ${\beta}$-galactosidase under the control of a polyhedrin promoter. The recombinant virus BmK1-104LacZ with the same genome structure as Bm101-LacZ, except for a p10 promoter region, produced a similar ${\beta}$-galactosidase activity to that due to Bm104-LacZ and 5.5-fold lower than that due to Bm101-LacZ. The virus yield, expression level of polyhedrin, and polyhedra productivity for each recombinant virus was almost similar. These results suggested that the difference in the expression level of ${\beta}$-galactosidase resulted from a difference in the p10 promoter regions, and that an expression vector using the p10 promoter of BmNPV-K1 could be usefully exploited in the mass production of foreign proteins with silkworm larvae by means of oral ingestion.

• Microbiology and Biotechnology Letters
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• v.18 no.3
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• pp.305-308
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• 1990

In order to clone the gene coding for 3-isopropylmalate dehydrogenase of Muyveromyces fragilis, a shuttle plasmid vector pHNll4 was used. It can serve as a cloning vector in Saccharomyces cerevisiae DBY746 for other Sau3AI-cleaved DNA segment of Kluyveromyces fragilis. Two cloned fragments which complement the leu2 mutation of Saccharomyces cerevisiae and E, coli were obtained. Their length was 4.4 kb an 3.5 kb, and their orientation was opposite each other. From the fact that the two recombinant plasmids were expressed in Saccharomyces cerevisiae and E, coli, probably the two inserts had the promoter of Ktuyveromyces fi-agilis and that of Kluyveromyces fiagilis was efficiently assosiated with RNA polymerase of Saccharomyces cerevisiae and E. coli. According to the result of Southern hybridization, we thought that the cloned fragment has low homology with 3-isopropylmalate dehydrogenase coding region of E. coli and Saccharomyces cerevisiae.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.2
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• pp.88-94
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• 2023

This study was conducted to analyze and compare the dry matter yield of Italian ryegrass (IRG) cultivated under monoculture and mixed culture system to recommend suitable varieties that can be cultivated. Italian ryegrass cultivars, Green Fram (GF, extremely early-maturing), Kowinearly (KE, early-maturing), Kowinmaster (KM, mild-maturing), and Hwasan 104 (H104, late-maturing), were used for mono or mixed cultivation. The average monthly temperature in Cheonan over the past 30 years tended to be similar, but that in November and March are judged to be abnormal weather. The dry matter yield of GF+H104 was significantly higher during harvest than that of GF (p<0.05). The dry matter yields of KE and KE+KM were significantly higher during harvest than the output standards of KE and KM. There was no significant difference between the dry matter yield of H104 and KM (p>0.05), but KM had the highest yield of 16,763.1 kg/ha. Analysis showed that the highest dry matter yield during IRG harvest was obtained under monoculture and KE+KM mixed culture. Because the occurrence frequency of abnormal weather such as drought during spring is increasing recently, it is judged that IRG cultivation using early and middle growth is necessary to prepare for abnormal weather.

• Korean Journal of Environmental Biology
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• v.18 no.3
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• pp.307-313
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• 2000

Aromatic hydrocarbons are known to be recalcitrant, so that they have been concerned as pollutant chemicals. Microorganisms play a major role in the breakdown and mineralization of these compounds. However, the kinetics of the biodegradation process may be much slower than desired from environmental consideration. The biodegradation of aromatic hydrocarbons is conducted by oxidation to produce catechol as a common intermediate which is metabolized for carbon and energy sources. In this study, a bacterial isolate capable of degrading several aromatic hydrocarbons was isolated from the contaminated wastewater of Yeocheon industrial complex. On the basis of biochemical characteristics and major cellular fatty acids, the isolate was identified as Pseudomonas putida Z104. The strain Z104 can utilize benzoate and catechol as the sole carbon and energy sources via a serial degradative pathway. The strain degraded actively 0.5 mM catechol in MM2 medium at pH 7.0 and 3$0^{\circ}C$.

• Proceedings of the Korean Magnestics Society Conference
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• 2008.12a
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• pp.104.1-104.1
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• 2008

• Analytical Science and Technology
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• v.13 no.4
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• pp.494-503
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• 2000

A 21-residue peptide corresponding to amino acids 84-104 of $p16^{INK4A}$, the tumor suppressor, has been synthesized and its structure was studied by Circular Dichroism, $^1H$ NMR spectroscopy and molecular modeling. A p16-derived peptide (84-104 amino acids) forming stable complex with CDK4 and CDK6 inhibits the ability of CDK4/6 to phosphorylate pRb in vitro, and blocks cell-cycle progression through G1/S phase as shown in the function of the full-length p16. Its NMR spectral data including NOEs, $^3J_{NH-H{\alpha}}$ coupling constants, $C_{\alpha}H$ chemical shift, the average amplitude of amide chemical shift oscillation and temperature coefficients indicate that the secondary structure of a p16-derived peptide is similar to that of the same region of full-length p16, which consists of helix-turn-helix structure. The 3-D distance geometry structure based on NOE-hased distance and torsion angle restraints is characterized by ${\gamma}$-turn conformation between residues $Gly^{89}-Leu^{91}$(${\varphi}_{i+1}=-79.8^{\circ}$, ${\varphi}_{i+1}=60.2^{\circ}$) as evidenced in a single crystal structure for the corresponding region of p18 or p19, but is undefined at both the N and C termini. This compact and rigid ${\gamma}$-turn region is considered to stabilize the structure of p16-derived peptide and serve as a site recognizing cyelin dependent kinase, and this well-defined ${\gamma}$-turn structure could be utilized for the design of anti-cancer drug candidates.

• KOREAN POULTRY JOURNAL
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• v.13 no.8 s.142
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• pp.104-106
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• 1981

• KOREAN POULTRY JOURNAL
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• v.3 no.12 s.26
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• pp.102-104
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• 1971