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Cadmium Adsorption and Exchangeable Cations Desorption in Soils: Effects of pH and Organic Matter Content (토양에서 카드뮴의 흡착과 치환성양이온의 탈착 : pH와 유기물함량의 영향)

  • 박병윤;신현무
    • Journal of Environmental Science International
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    • v.5 no.2
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    • pp.243-252
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    • 1996
  • In order to investigate the effects of pH and organic matter content on cadmium adsorption and exchangeable cations desorption in soils, the adsorption isotherms of cadmium and the desorption isotherms of calcium and magnesium on four New jersey soils at four pH values were plotted, and the cadmium partition coefficients (Kd) were also calculated. The slopes of cadmium adsorption isotherms dramatically increased with increasing solution pH. Judging from Langmuir adsorption equations, the maximum adsorption quantities(b) of cadmium at high pH values were much greater than those at low pH values for the same soil. The partition coefficients increased greatly with increasing solution pH. The slopes of regression equations between partition coefficients and pH values were steep in the order of the organic matter content of the soils. The correlation coefficients (r2) between partition coefficient and organic matter content for soils. The correlation coefficients (r2) between partition coefficient and organic matter content for $1\times10^{-4}$M increased from 0.3027 at pH 4.0 to 0.9964 at pH 8.5 and from 0.2093 at pH4.0 at 0.9657 at pH 8.5 for$2\times10^{-4}$M ${Cd(NO_3)}_2$. The desorption quantities of calcium and magnesium decreased with increasing solution pH and increased with- increasing cadmium adsorption.

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The retrogradation and swelling power of modified potato starches (변성 감자 전분의 노화와 팽윤력)

  • Kim, Ji-Tae;Noh, Wan-Seob
    • Applied Biological Chemistry
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    • v.35 no.5
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    • pp.404-409
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    • 1992
  • Hydroxypropylated starches and acetylated starches were prepared by reaction of potato starch with propylene oxide and acetic anhydride, respectively and then degree of retrogradation and swelling power were investigated in different pHs and temperature. The extent of retrogradation determined by glucoamylase method during freeze-thaw treatment and storage in low temperature $(0{\sim}5^{\circ}C)$ showed that modified potato starches were slowly retrograded as the increase of degree of substitution. The order of the retrogradation tendencies in different pHs were pH 4>pH 6>pH 8>pH 10. Retrogradation of hydroxypropylated potato starches were less than that of acetylated potato starches. Swelling power of starches were influenced more by the temperature than by the pH. The order of the swelling power tendencies in different pHs were pH 10>pH 2>pH 8>pH 4>pH 6.

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Effect of Temperature, Time and pH on the Extraction of Protein in a Chrysalis of Silk Worm. (누에 번데기의 단백질 추출에 관한 연구)

  • 조철형;차월석;차월석
    • KSBB Journal
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    • v.4 no.2
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    • pp.65-68
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    • 1989
  • In order to determin the optimum extraction condition for protein in a Chrysalis of Silk Worm, Temperature, Time, and pH variation were conducted in the extraction. N.S.I. and nitrogen contents of the extracts in this condition were identified by Kjeldaha method and a C.H.N. corder. The results were as follows; Crude protein and crude fat contents in the extracts were 23.34% and 15.61%, respectively. N.S.I. values with respect to the temperature variation were 6.7% at 6$0^{\circ}C$, 7.94% at 8$0^{\circ}C$ and 8.67% at 10$0^{\circ}C$ in the condition of pH 4, 1 hr. N.S.I. values in pH variation were 7.94% at pH4, 8.99% at pH6, 9.70% at pH8, 10.19% at pH 10 and 12.16% at pH 12 in the condition of 8$0^{\circ}C$, 1hr. N.S.I. values in extraction time variation were 8.67% in 1hr, 9.23% in 2hr and 9.76% in 3hr in the condition of 10$0^{\circ}C$, pH 4. The tendency of N.S.I. variation in this extration condition was reconfirmed also by a C.H.N. corder.

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Influence of Dietary Salinomycin on Feeding-induced Variations of Glucose Kinetics and Blood Volatile Fatty Acids and Insulin Concentrations in Sheep Fed a High-roughage Diet

  • Fujita, Tadahisa;Itoh, Takahiro;Majima, Hiroya;Sano, Hiroaki
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.3
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    • pp.365-372
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    • 2007
  • This study was conducted to determine effects of salinomycin (SL) on feeding-induced changes in glucose kinetics and blood VFA concentrations in sheep fed a high-roughage diet. Four sheep were fed the diet with or without 20 mg/kg diet of SL once daily for 21 d. Glucose entry and utilization rates were determined during the prefeeding and 3 h postfeeding periods, using a [$^{13}C_6$]glucose dilution method and non-steady state equations. Ruminal characteristics and concentrations of blood VFA, plasma glucose and insulin were also measured during the same periods. A feeding-induced increase in ruminal total VFA concentration tended to be inhibited (p<0.10) with SL, although ruminal pH was unaffected (p>0.10) with SL or by feeding. Salinomycin decreased (p<0.05) acetate proportion and increased (p<0.05) propionate proportion in the rumen, but did not modify these changes in response to feeding (p>0.10). A feeding-induced increase in blood acetate concentration was attenuated (p<0.05) with SL. Salinomycin tended to increase (p<0.10) blood propionate concentration without modifying its response patterns to feeding (p>0.10). Plasma concentrations of glucose or insulin were unaffected (p>0.10) with SL. Salinomycin tended to enhance (p<0.10) glucose entry and utilization rates. Feeding also enhanced (p<0.01) both rates, whereas their interactive effect was not detected (p>0.10). We conclude that SL possibly enhances whole body glucose entry and utilization with an increase in blood propionate concentration in sheep given a high-roughage diet, although SL does not appear to affect their responses to feeding.

Fluoride Removal from Aqueous Solution Using Thermally Treated Pyrophyllite as Adsorbent (고온 처리된 납석을 흡착제로 이용한 용액상의 불소 제거)

  • Kim, Jae-Hyun;Song, Yang-Min;Kim, Song-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.2
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    • pp.131-136
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    • 2013
  • The aim of this study was to investigate the removal of fluoride using thermally treated pyrophyllite as adsorbent. Sorption experiments were conducted under batch conditions to examine the effects of adsorbent dose, reaction time, initial fluoride concentration and solution pH on fluoride removal. In the experiments, the pyrophyllite thermally treated at different temperatures [untreated (P-U), $400^{\circ}C$ (P-400), $600^{\circ}C$ (P-600)] were used. Results showed that the adsorption capacity was in the order of P-400 > P-U > P-600. The XRD analysis indicated that both P-U and P-400 were composed of quartz, dickite and pyrophyllite while P-600 was quartz. The BET analysis showed that the specific surface area was in the order of P-600 > P-400 > P-U. Kinetic data showed that fluoride sorption to P-400 arrived at equilibrium around 24 h. Equilibrium test demonstrated that the maximum sorption capacity of P-400 was 0.957 mg/g. In addition, fluoride removal by P-400 was not sensitive to solution pH between 4 and 10. However, fluoride removal decreased considerably at highly acidic (pH < 4) and alkaline (pH > 10) conditions. This study demonstrates that pyrophyllite could be used as a low-cost adsorbent for fluoride removal from aqueous solution.

Characterization of Mitochondrial Plasmids from Pleurotus spp. (Pleurotus속 균주들의 미토콘드리아 플라스미드 특성)

  • 김은경;구용범;차동렬;하영칠;노정혜
    • Korean Journal of Microbiology
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    • v.31 no.2
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    • pp.141-147
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    • 1993
  • Plasmid DNAs were detected from the mitochondrial fraction of four strains of whiterot fungus, Pleurotus ostreatus. The size of the plasmids were 10.2 and 7.2 kb in strain NFFA 2, 10.2 kb in NFFA 4001, 11.2 kb in NFFA 4501, and 10.2 and 11.2 kb in KFCC 11635. The two strains,NFFA 2ml and NFFA 2m2, which are mutant derivatives of NFFA 2, did not contain any plasmids. The cleavage by proteinase K indicated that these plasmids have DNA ends associated with proteins. In digestion with proteinase K all the plasm ids remained resistant to lambda exonuclease which hydrolyzes DNA from 5' ends and were sensitive to exonuclease III which hydrolyzes DNA from 3' ends. This suggests that the plasmids are linear double-stranded DNA and the terminal proteins are covalently linked to 5' ends of plasm ids. In order to find relationship between these plasmids, hybridization of plasm ids by each separate plasmid DNA was done. The result indicated that the plasmids can be classified into at least 3 groups. Plasmids of group I were present in all the P ostreatus. More mitochondrial plasmids were detected in P cornucopiae. P ,florida, P pulmonarius, P sajor-caju, and P spodoleucus. The size of plasmids ranged between 7.2 kb and 14 kb. All the species except P cornucopiae contained plasmids of approximately 10 kb which hybridized with the 10.2 kb plasmid (group I) of P ostreatus NFFA 2.

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Effect of pH on Growth and Cultural Characteristics of Bacillus sp. SH-8 and Bacillus sp. SH-8M (Bacillus sp. SH-8과 Bacillus sp. SH-8M의 생육 및 배양 특성에 미치는 pH의 영향)

  • 심창환;신원철;유주현
    • Microbiology and Biotechnology Letters
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    • v.20 no.4
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    • pp.371-376
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    • 1992
  • The growth and cultural characteristics of Bacillus sp. SH-8 and SH-8M were investigated at various pH conditions. Bacillus sp. SH-8 showed normal growth pattern above pH 9.0. However, with the pH adjusted below 7.7, 0.$D_{550}$ decreased rapidly with concomitant reduction in viable cell numbers. In contrast, Bacillus sp. SH-8M demonstrated growth capability at pH 7.7, but with slightly reduced growth rate at pH 6.9. Similar results were obtained when those two strains were cultivated on the solid medium. Both of them showed short rod shapes at pH 10.2. However, at pH 7.7 only Bacillus sp. SH-8 was observed to have elongated rod shape. Extracellular pH of both the strains, when cultured at initial pH of 10.2, reached to 9.0 after the incubation of 28 hours. At the initial pH of 9.0 and 9.6, the extracellular pH was reduced at the beginning of cultivation, but elevated after 12 hours. When cultured at initial pH of 6.9 and 7.7, extracelluar pH of Bacillus sp. SH-8M increased to 8.0 and 8.7, respectively, while that of Bacillus sp. SH8 remained constant pH 7.0. The highest sporulation rate of Bacillus sp. SH-8 and SH-8M was obtained at the initial pH of 10.2 and after the incubation of 3 days with the sporulation rate of 95% and 85%, respectively.

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Effects of Different Performance Sequences of Aerobic and Resistance Exercises for 10 Weeks on Body Composition, Physical Function and Hormones in Males aged 20s (10주간 유산소와 저항성 운동의 다른 수행 순서에 따른 20대 남성의 신체조성, 신체기능과 호르몬에 미치는 영향)

  • Kim, Daeyeol;Jeong, Jaekwan;Cho, Sungchae;Kuk, Doohong;Park, Hyeok;Lee, Hayan;Hong, Goeun;Hwang, Yeonhee;Kim, Donghee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.1
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    • pp.446-455
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    • 2018
  • This study examined the effects of different performance sequences of aerobic and strength exercises for 10 weeks on the body composition, physical function, and hormones in males aged in their 20s. A total of 30 subjects (N=30) were assigned randomly to either aerobic and resistance exercise (n=10, A+R), resistance and aerobic exercise (n=10, R+A), or a control group (n=10, CON). The different order of aerobic (HRmax 50-80%, 30 min) and resistance (50-80% 1RM, 30 min) exercises for 10 weeks was consisted of 3 times per week and 80 minutes per session. The body composition, physical function, and hormones were measured before and after the training period. The lean body mass (p=.015) was increased and the fat mass (p=.042) and % body fat (p=.007) in the A+R were decreased. The skeletal muscle mass (p=.001) in the R+A was increased and % body fat was decreased (p=.003). The weight (p=.03) and % body fat (p=.039) in the CON were increased. The aerobic capacity (p=.011) and muscular endurance (p=.001) in the A+R group were improved. The muscular endurance (p=.0016) in the R+A was improved. The epinephrine (p=.048), norepinephrine (p=.013), and cortisol (p=.045) levels in the A+R group were increased. The epinephrine (p=.046) level in the R+A group was increased. The insulin (p=.007) level increased in the CON group. In conclusion, both A+R and R+A groups produced superior results to the CON group but the A+R group was slightly more efficient than the R+A group.

Evaluation of Phosphorus Removal Efficiency at Various Coagulation Conditions Using Polyaluminum Chloride with Different Al Contents (Al 함량이 다른 PAC를 이용한 응집 조건 별 인 제거효율 평가)

  • Jeong-Hak Choi;Geon-Gon Yoon;Chang-Han Lee
    • Journal of Environmental Science International
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    • v.32 no.10
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    • pp.731-739
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    • 2023
  • In this study, lab-scale phosphorus coagulation/precipitation experiments were performed using three types of polyaluminum chloride (PAC) with different Al contents (10%, 12%, and 17%). The PO4-P removal efficiencies at various operating conditions, such as initial PO4-P concentration, initial pH, and Al/P molar ratio, were evaluated, and correlations among the operating factors affecting phosphorus coagulation/precipitation with PAC were derived to optimize the process efficiency. When the initial PO4-P concentration was 0.065 and 0.161 mmol P/L under an initial pH of 8-10, the optimal PAC dose was 0.126-0.378 and 0.189-0.667 mmol Al/L, respectively. Under these conditions, the Al/P molar ratio was 2.16-6.18 and 1.28-4.30, respectively, and the PO4-P removal efficiency was in the range of 40.2-92.5%. When the Al/P molar ratio was 2 or less under an initial pH condition of 6-8, the PO4-P removal efficiency was approximately ≤40% owing to insufficient Al3+ ions. However, when the Al/P molar ratio is 3-5, the PO4-P removal efficiency improved to approximately 80-90%. Thus, the optimal Al/P molar ratio to achieve a PO4-P removal efficiency of over 90% was determined to be approximately 4 in the PO4-P coagulation/precipitation process using PAC.

The impact of two doses of coenzyme Q10 on semen parameters and antioxidant status in men with idiopathic oligoasthenoteratozoospermia

  • Alahmar, Ahmed T
    • Clinical and Experimental Reproductive Medicine
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    • v.46 no.3
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    • pp.112-118
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    • 2019
  • Objective: Oxidative stress contributes to male infertility, and antioxidants have been recommended for treating idiopathic oligoasthenoteratozoospermia (OAT). There is, however, a lack of agreement on the type, dosing, and use of individual antioxidants or combinations thereof. The purpose of this study was to compare the effects of two doses of coenzyme Q10 (CoQ10) on semen parameters and antioxidant status in men with idiopathic OAT. Methods: In this prospective study, patients with idiopathic OAT received 200 mg/day (n = 35) or 400 mg/day (n = 30) of CoQ10 orally for 3 months. All patients underwent semen analysis according to the fifth editions of the World Health Organization criteria. Total antioxidant capacity (TAC), catalase (CAT) activity, and superoxide dismutase (SOD) activity were measured both before and after treatment. Results: Treatment with CoQ10 (200 mg/day or 400 mg/day) resulted in a significant increase in sperm concentration from baseline ($8.22{\pm}6.88$ to $12.53{\pm}8.11million/mL$, p= 0.019; $7.58{\pm}5.41$ to $12.33{\pm}6.1million/mL$, p= 0.002, respectively), progressive motility ($16.54%{\pm}9.26%$ to $22.58%{\pm}10.15%$, p=0.011; $14.22%{\pm}12.85%$ to $26.1%{\pm}14.52%$, p= 0.001, respectively), and total motility ($25.68%{\pm}6.41%$ to $29.96%{\pm}8.09%$, p= 0.016; $23.46%{\pm}12.59%$ to $34.82%{\pm}14.17%$, p= 0.001, respectively). CoQ10 therapy also increased TAC (p= 0.009, p= 0.001, respectively), SOD activity (p= 0.004, p= 0.001, respectively), and CAT activity (p= 0.039, p= 0.024, respectively). Furthermore, antioxidant measures correlated significantly with seminal fluid parameters (r = 0.36-0.76). Conclusion: CoQ10 supplementation improved semen parameters and antioxidant status in men with idiopathic OAT, with a greater improvement shown in men who took 400 mg/day than in those who took 200 mg/day.