• 한국임상수의학회지
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• 제23권3호
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• pp.272-278
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• 2006

This study was carried out to determine therapeutic the effect of ozonated water therapy on bovine pododermatitis. In addition, bactericidal effect of ozonated water on etiological agent of bovine pododermatitis was examined. The pathohistological examination for the pododermatitis, according to application with ozonated water and ozone ointment was investigated. Thirty healthy cattle were divided two groups(each of 15) : control group(povidone group), treatment group(ozone solution group). Various parameters were evaluated in terms of the lameness score, swelling score, lesion score, WBC count, neutrophil count, pathohistological finding, and antimicrobial action. The decrease of lameness and lesion score were shown in hoof lesions on 14 days after application of ozonated water. Significant decrease of swelling was shown in hoof lesions on 14 days 1Corresponding author after application of ozonated water(p<0.01). In hematological findings, WBC count revealed values within normal range. The number of neutrophils was slightly higher than that of normal, however, this was improved on 14 days after application of ozonated water. In pathohistological findings, recovery was rapid macroscopically and microscopically in the treatment with ozonated water on the hoof lesions and ozonated water was effective. In antimicobial action, bactericidal effect was observed in treatment with ozonated water on the hoof lesions and ozonated water was effective.

• 한국환경과학회지
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• 제16권12호
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• pp.1425-1430
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• 2007

This study was conducted to investigate the effect of ozonated water and ozonated water+hydrogen peroxide treatment of residual procymidone in perilla leaf containing 20 mg/L procymidone. Samples was treated with ozonated water containing 1.0, 2.0 and 3.0 mg $O_3/L$ ozone and hydrogen peroxide water containing 1.0, 2.0 and 3.0 mg $H_2O_2/L$ hydrogen peroxide in pH 5, 7 and 9, respectively, at $15^{\circ}C$. Procymidone removal rate was 26.5% in 7 days at $15^{\circ}C$ and optimum condition of procymidone removal was the case of treating with ozonated water containing 2.0 mg $O_3/L$ and pH 9. As the result procymidone removal rate was about 96.5%. In this case of adding hydrogen peroxide, optimum condition of procymidone removal was $1:0.5{\sim}1(O_3:H_2O_2)$. However, procymidone was nearly removed with the treatment of hydrogen peroxide water only.

• Preventive Nutrition and Food Science
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• 제9권2호
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• pp.121-125
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• 2004

Effects of chemical treatment with a citric acid solution or ozonated water on microbiological changes in lettuce and cabbage during storage were studied. Fresh lettuce and cabbage samples were cut into small pieces and treated by soaking in either ozonated water or a citric acid solution. After treatment, populations of total bacteria, yeast and mold, and E. coli were determined. Numbers of microorganisms increased during storage, but ozonated water and citric acid treatments retarded the increase in microbial growth. Among treatments, 1 % citric acid treatment was the most effective in terms of microbiological change and inhibition of polyphenoloxidase (PPO). For lettuce, citric acid treatment decreased the microbial growth overall by 1.5 log CFU/g and inhibited the PPO activity by 80%. These results indicate that chemical-treated lettuce and cabbage retained a better quality than those of the control during storage.

• 산업식품공학
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• 제14권4호
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• pp.316-321
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• 2010

본 연구는 식중독을 일으키는 병원성 미생물들은 식품시료와 교차오염 원인의 지표가 될 수 있는 도마에 접종한 후 오존수와 일반 수돗물을 이용하여 세척한 후 미생물의 변화와 세척방법에 따른미생물의 변화를 각각 비교 관찰하였다. 실험결과 일반 수돗물보다 오존수가 살균효과가 더 높았고, 같은 세척수로 세척 시에도 접종 방법, 실험시료 및 시료의 표면, 균질화에 따라 살균효과가 다르게 나타나는 것을 확인할 수 있었다. 세척방법으로는 침수보다 주수 에서 살균효과가 더 컸으며 오존수의 농도는 0.2 ppm에서 유의적인 살균효과를 나타내었으며 오존수 농도가 0.4, 0.6, 1.0 ppm으로 증가함에 따라 농도 의존적으로 살균효과 또한 증가하였다. 위의 결과들을 통해 0.2 ppm 이상의 오존수가 부패 미생물 제어에 미치는 영향이 크고 식품의 제조 가공 시 여러 안전성 면에 있어서 오존수를 이용한 세척이 효과적이라고 생각된다.

• 한국축산식품학회지
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• 제31권4호
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• pp.617-623
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• 2011

쇠고기를 오존수(0.2 ppm)로 0, 5, 10, 30, 60분간 침수 처리하여 $5^{\circ}C$에서 24일 동안 저장하면서 3일 간격으로 쇠고기의 품질 측정 지표라 할 수 있는 휘발성 염기태질소(volatile basic nitrogen, VBN), 지방산패도(thiobarbituric acid reactive substances, TBARS), 산가(acid value, AV), pH 를 측정하여 오존수 처리가 냉장 저장중의 쇠고기의 품질에 미치는 영향을 평가하였다. 휘발성 염기태질소와 pH 변화를 측정한 결과 오존수 비처리구는 9일째 부패로 인정되는 수치가 측정된 반면 오존수(0.2 ppm)로 60분간 처리한 쇠고기에서는 15일까지 안전한 수치가 측정되었다. 지방산패도와 산가 또한 모두 오존수 처리한 쇠고기가 비처리구 쇠고기보다 낮은 측정치를 나타내었다. 본 실험의 내용을 종합한 결과 저 농도 오존수가 냉장 쇠고기 화학적 특성에 영향을 끼치며 저장성 향상에 효과적이라고 판단된다.

• 한국임상수의학회지
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• 제23권1호
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• pp.14-17
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• 2006

본 연구에서는 충남 공주근교에서 사육중인 한국재래산양에서 자연 발생한 전염성 농포성 피부염에 대하여 오존의 치료효과를 조사하였다. 전염성 농포성 피부염은 한국재래산양 150두 중 130두에서 발생하였다. 16두(2개월-2년)를 선발하여 대조군(5두), ozonated oil군(5두) 및 ozonated water군(6두)으로 구분하였다. 대조군에서는 실험기간 중 아무런 치료를 하지 않았으며, ozonated oil군은 식물성유에 오존가스(200ppm)로 3일간 bubbling한 ozonated oil을 환부에 1일 1회 2주간 도포하였고, ozonated water군은 수도물에 오존가스(200ppm)로 1시간 동안 bubbling한 ozonated water(0.1ppm)를 2주간 자유로이 음수토록 하였다. 그 결과, 치료율은 ozonated oil군이 80%(4/5두), ozonated water군이 66.7%(4/6두)로, 대조군(20%, 1/5두)보다 양호하였다. 이 결과를 근거로 전염성 농포성 피부염에 이환된 한국재래 산양 121두를 대상으로 ozonated oil을 1일 1회, 30일간 환부에 도포하였다. 그 결과, 환축의 모든 병변부는 완치되었다. 이상의 결과를 종합해 볼 때, 오존요법은 산양의 전염선 농포성 피부염의 치료에 유효한 것으로 판단되었다.

• Fisheries and Aquatic Sciences
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• 제21권6호
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• pp.15.1-15.9
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• 2018

Background: Inflammation has been known to associate with many human diseases. The objective of this study was to evaluate an anti-inflammatory effect of ozonated krill (Euphausia superba) oil, which was prepared by the treatment of krill oil using ozone gas. The anti-inflammatory activity was evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Results: Ozonated krill oil significantly inhibited nitric oxide (NO) production and suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 macrophages. Ozonated krill oil also reduced the mRNA expression of inflammatory cytokines such as interleukin (IL)-$1{\beta}$, IL-6, and tumor necrosis factor (TNF)-${\alpha}$ in LPS-stimulated RAW 264.7 macrophages. To elucidate the mechanism underlying the anti-inflammatory activity of ozonated krill oil, we evaluated the effects of ozonated krill oil on the activation of mitogen-activated protein kinases (MAPKs) pathway. Ozonated krill oil suppressed the LPS-stimulated phosphorylation of p38 MAPK and c-Jun N-terminal kinases (JNK). Conclusion: This study revealed that the ozonated krill oil exhibited an anti-inflammatory effect in LPS-stimulated RAW 264.7 macrophages. To the best of our knowledge, this is the first report that ozonated krill oil suppressed pro-inflammatory mediator and cytokine expression in LPS-stimulated RAW 264.7 macrophages by inhibiting the phosphorylation of p38 MAPK and JNK.

• 한국식품저장유통학회지
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• 제3권2호
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• pp.145-148
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• 1996

This paper was carried out to investigate change in the initial microorganism of Pepper which were treated with ozonated water for microbial decontamination. When ozonated water was 30, 60, 90minutes treatment, there was decrease in the overall total microbial of the black & white pepper. Total lactic acid bacteria died completely after contacted with ozonated water for 60minutes but the inhibition rate on the total aerobic bacteria of black & white pepper were 62% and 96%, respectively. Total aerobic bacteria of four packing material was increased after 1 months of storage at 37$^{\circ}C$ when it had treated with 90minutes ozone reaction Then, there was no significant changes in the piperin contents.

• 펄프종이기술
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• 제43권1호
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• pp.29-35
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• 2011

Since the role of lignin in the wood cell wall is to keep integrity and structure rigidity of lignocellulosic substrate, lignin of the cell wall has to be destroyed before enzymatic hydrolysis of wood polysaccharides. The aspen wood meals were delignified with ozone in acidic condition. The chemical characteristics of wood meal were investigated. The 60% of lignin and almost zero % of polysaccharides in aspen wood meal was degraded with 10min. ozone treatment. The phenolic hydroxyl groups of lignin in ozonated wood meal were increased with ozonation time. The sugar composition of ozonated wood meal showed that the hemicellulose was more susceptible to ozonation than cellulose. The yield of aldehyde was increased in some degree with 10min. ozone treatment and decreased with longer ozone treatment.

• 한국응용과학기술학회지
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• 제35권4호
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• pp.1407-1412
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• 2018

본 연구는 오존화 올리브오일이 독성이 적으면서 다양한 종류의 미생물을 치사시킬 수 있으나 경우투여나 복강투여에도 인체에 해가 없는 것으로 알려져 있다. 그리고 국내에서는 아직 동물에 대한 안정성 실험이 없었다. 그래서 본 연구에서는 오존화 올리브오일을 사용하여 동물의 안구를 통한 안정성 실험을 하였다. 구체적인 연구에서는 rabbit에서 오존화오일(고농도)의 동물 눈 안점막 자극을 평가하기 위해 실시하였고, 시험물질 처치부위를 관찰한 결과, 비세척군 및 세척군 모두에서 안점막 자극이 관찰되지 않았다.