• 한국식품과학회지
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• 제28권1호
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• pp.184-189
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• 1996

냉이(Capsella bursa-pastoris)로부터 산소독성에 영향을 미치는 superoxide anion radical에 대하여 소거작용을 하는 물질을 분리하고 이 물질의 이화학적 성질을 검토하였다. 냉이를 에탄올로 추출한 후 용매분획과 각종 column chromatographies (Amberlite XAD-2, Sephadex LH-20, Bio gel P-2, ODS)를 통해 정제하였고, 최종적으로 FPLC (Fast protein liquid chromatography)를 사용하여 활성의 단일물질을 얻었다. 냉이 에탄올추출물 100 g으로부터 0.25 g의 정제물질을 얻었으며, superoxide anion radical을 50% 소거하는 농도$(IC_{50})$는 $0.58\;{\mu}g$이었다. 이 물질에 대해 각종 이화학적 성질을 검토한 결과 phenol성 화합물의 배당체로 추정하였다.

• 한국추진공학회지
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• 제14권4호
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• pp.46-58
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• 2010

최근 들어 새로운 차세대 우주발사체의 추진기관의 개발 방향에서 친환경성과 저비용성이 주요 관심사이며, 과산화수소와 액체메탄은 이러한 추진제로 대표적인 관심을 받고 있다. 과산화수소는 로켓 개발 초기에 널리 사용되었으나, 냉전시대에 성능을 최우선시함에 따라 사용이 현격히 감소해오다가 1990년대 이후 안정성의 향상과 더불어 다시 재조명 받기 시작하였다. 액체메탄은 실제 발사체의 추진기관으로 사용되지는 않았지만, 액화천연가스(LNG)로 사용할 수 있기 때문에 친환경/저비용 추진제로 지속적으로 관심을 받아왔으며 최근에는 달/화성 탐사와 관련하여 지속적인 연구가 수행되고 있다. 이러한 대표적인 친환경 추진제에 대한 활용 역사와 최근 연구 동향을 고찰하여, 향후 국내에서 소요될 각종 임무 조건을 만족하는 엔진의 연구 개발 방향 설정에 도움이 될 것으로 예상된다.

• Ocean and Polar Research
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• 제43권3호
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• pp.141-148
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• 2021

Microplastic contamination in waterbodies is a growing source of concern for researchers and other stakeholders. We investigated oxidative stress and toxicity in goldfish (Carassius auratus) in response to exposure to 1-㎛ diameter micro-polystyrene (MP) at concentrations of 0, 10, 100, and 1000 beads/mL (MP 0, MP 10, MP 100, and MP 1000 groups) for 7 d (at day 0, 1, 3, 5, and 7). We analyzed the survival rates; superoxide dismutase (SOD) and catalase (CAT) mRNA expression levels in the liver; SOD and CAT activity in the plasma; caspase-3 mRNA expression in the liver; and the levels of hydrogen peroxide (H₂O₂) in plasma. Terminal transferase dUTP nick end labeling (TUNEL) assays were also conducted to determine apoptosis levels in the liver. All fish in the MP 1000 group died by day 7 and the MP 100 group had a lower survival rate than the MP 10 and MP 0 groups. The mRNA expression as well as SOD, CAT, and caspase-3 activity levels were increased significantly with increases in MP concentration and exposure time. Finally, according to the TUNEL assay, more apoptosis was observed in the MP 1000 group at day 5 than in other groups. In summary, MP concentrations above 100 beads/mL caused death and oxidative stress to goldfish. We conclude that MP can cause oxidative stress and apoptosis in goldfish, which leads to death.

• 한국식품과학회지
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• 제53권2호
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• pp.165-173
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• 2021

본 연구에서는 UPM에 의해 유발되는 NCI-H441 세포 내 ROS 생성과 그에 따른 tight junction 단백질(N-cadherin, fibronectin, occluding, ZO-1, 및 claudin-4 등)의 발현 억제를 확인하였고 ECL의 전처리로 인하여 해당 ROS 생성 및 tight junction 단백질 발현 억제 현상이 완화되는 보호효과를 확인하였다. 그 결과, 폐 세포에서 UPM에 의해 유발된 산화 스트레스 및 tight junction 단백질의 발현 감소가 ECL을 선 처리함으로써 억제되어 결과적으로 산화 스트레스와 같은 미세먼지에 의한 폐 손상으로부터 보호 효과를 나타내고 tight junction 수준의 유지를 확인하였다. 이러한 결과를 통해 ECL 추출물은 폐와 기관지의 보호효과를 나타내며 폐 관련 질병의 예방효과가 있는 기능성 천연 물질로써 활용이 가능할 것으로 기대된다.

• 한국버섯학회지
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• 제18권4호
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• pp.365-371
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• 2020

이 연구에서는 Perenniporia fraxinea를 사용하여 배지가 카르테노이드 생성 및 균사 성장에 미치는 영향을 분석했습니다. 0.1% 미만의 펩톤을 함유한 맥아추출물은 카르테노이드의 생성을 촉진했으며 0.2% 이상의 펩톤이 그 생산을 억제하는 것으로 나타났다. 맥아추출물이 없으면 Perenniporia fraxinea는 소량의 펩톤 첨가에도 불구하고 카르테노이드를 생산하지 못했다. 아카시재목버섯의 배양을 통하여 생산된 균사체를 원심분리로 배양액을 분리하여 상층액을 카르테노이드 용액으로 회수가 가능하였다. 균사체 내부에 축적된 카르테노이드는 에탄올을 사용하여 추출하였다. 배양액에서 분리 또는 추출한 카르테노이드 용액은 우수한 DPPH 라디칼 소거 활성을 나타냈다. 본 연구에서 생산된 항산화 카르테노이드는 천연물 유래 물질로 독성 및 부작용이 없으며 항산화 효과가 우수하여 활성산소에 의해 생성된 산화물을 효과적으로 제거할 수 있다.

• Nutrition Research and Practice
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• 제16권2호
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• pp.173-193
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• 2022

BACKGROUND/OBJECTIVES: Alzheimer's disease (AD) is one of the most representative neurodegenerative disease mainly caused by the excessive production of amyloid beta (Aβ). Several studies on the antioxidant activity and protective effects of Populus tomentiglandulosa (PT) against cerebral ischemia-induced neuronal damage have been reported. Based on this background, the present study investigated the protective effects of PT against cognitive impairment in AD. MATERIALS/METHODS: We orally administered PT (50 and 100 mg/kg/day) for 14 days in an Aβ_25-35-induced mouse model and conducted behavioral experiments to test cognitive ability. In addition, we evaluated the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum and measured the production of lipid peroxide, nitric oxide (NO), and reactive oxygen species (ROS) in tissues. RESULTS: PT treatment improved the space perceptive ability in the T-maze test, object cognitive ability in the novel object recognition test, and spatial learning/long-term memory in the Morris water-maze test. Moreover, the levels of AST and ALT were not significantly different among the groups, indicating that PT did not show liver toxicity. Furthermore, administration of PT significantly inhibited the production of lipid peroxide, NO, and ROS in the brain, liver, and kidney, suggesting that PT protected against oxidative stress. CONCLUSIONS: Our study demonstrated that administration of PT improved Aβ_25-35-induced cognitive impairment by regulating oxidative stress. Therefore, we propose that PT could be used as a natural agent for AD improvement.

• 대한본초학회지
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• 제28권6호
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• pp.111-117
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• 2013

Objectives : This research aimed at studying the immuno modulating activity of Fermented Epimedii Herba (EHS). Method : The impacts on the cell viability, hydrogen peroxide and nitric oxide (NO) generation in cells, and cytokines such as tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin (IL)-6, IL-$1{\beta}$, monocyte chemoattractant protein-1 (MCP-1) level have been measured by using Raw 264.7 cells with the specimen EHS as the fermented extract of Epimedii Herba with Saccharomyces cerevisiae STV89. Result : As a result of MTT assay to confirm the cytotoxicity of extracts from fermented Epimedii Herba, the toxicity was not excessively induced in Raw 264.7 cells when EHS were processed by concentration. EHS increased hydrogen peroxide generation in Raw 264.7 cells. EHS suppressed NO generation in Raw 264.7 cells while they significantly suppressed the increase of NO generation induced by LPS in macrophage. EHS significantly decreased the generation amount of TNF-${\alpha}$ and IL-6 induced by LPS in Raw 264.7 cells at $25{\mu}g/mL$ or more. Conclusion : It appeared that the fermented extract of Epimedii Herba manufactured from Epimedii Herba significantly has the immuno modulating acitivity as it did not excessively trigger cytotoxicity to Raw 264.7 cells, increased hydrogen peroxide generation in Raw 264.7 cells, decreased NO generation in macrophage, and especially, suppressed both TNF-${\alpha}$ and IL-6 generation in macrophage induced by LPS.

• 한국식생활문화학회지
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• 제38권2호
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• pp.112-118
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• 2023

Seaweed-derived foods have long been popular in Korea because of their high content of nutrients that are beneficial to the human body. Recently, Korean seaweeds have been used as raw materials to produce new natural products with health benefits. Herein, we compared the antioxidant activity of 16 Korean seaweed extracts to explore their potential utility as health foods. The total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of seaweed extracts were determined. We also investigated their ability to protect human diploid fibroblast (HDF) cells against hydrogen peroxide. The results showed that seaweed extracts at a concentration of 100 ㎍/mL did not cause any cell toxicity. Sargassum thunbergii (Jichung-i) had the highest TPC and radical scavenging effects, followed by Porphyra tenera (Gim), Silvetia siliquosa (Tteumbugi), and Sargassum fusiforme (Tot). Hydrogen peroxide increased the production of intracellular reactive oxygen species, while P. tenera (Gim), Saccharina japonica (Dasima), and S. thunbergii (Jichung-i) extracts significantly decreased it. The effect was highest in the S. thunbergii (Jichung-i)-treated HDF cells. These findings indicate that S. thunbergii (Jichung-i) shows promise as a potential antioxidant raw material.

• 대한본초학회지
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• 제38권4호
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• pp.45-52
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• 2023

Objectives : Fine dust caused by environmental pollution cause oxidative damage and skin aging. In this study, The possibility of using the Codium fragile extract (CFE) as an anti-aging product for skin improvement was evaluated by confirming the protective effect of skin cells from PM10 (particulate matter 10) through inhibition of ROS and MMPs. Methods : In this study, elastase and collagenase inhibitory activities were evaluated. Cell viability was evaluated by treating keratinocytes (HaCaT cell line) with CFE at various concentrations. The cytoprotective effect from PM10 in keratinocyteswas evaluated using the 3-[4,5-dimethylthiazol]-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay. ROS (reactive oxygen species) was measured in keratinocytes damaged by PM10 using DCF-DA (2′,7′-dichlorofluorescin diacetate) fluorescence staining. As an anti-aging effect of CFE, MMP-1 (matrix metalloproteinase-1) and MMP-1 (matrix metalloproteinase-9) inhibitory activities were evaluated. Results : As a result, CFE decreased the activity of elastase and collagenase. As a result of evaluating the toxicity of CFE, it is non-toxic at a concentration of 10 to 80 ㎍/㎖. Although cell viability of HaCaT cells treated with PM10 decreased, cell viability increased by 38% when treated with CFE 80 ㎍/㎖. Also, ROS decreased by 8.4%, and MMP-1 and MMP-9 decreased at CFE 80 ㎍/㎖. Conclusions : CFE showed excellent cell protection effect, and it is considered that it can be used in anti-aging products for skin improvement by effectively inhibiting ROS and MMPs from keratinocyte damage caused by fine dust.

• 한국수산과학회지
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• 제57권3호
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• pp.227-238
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• 2024

This study aimed to evaluate the effects of polyethylene microplastic (PE-MPs) via measuring the growth performance, hematological parameters, and antioxidant responses in Korean rockfish Sebastes schlegelii exposed to waterborne polyethylene microplastic with dimensions of 22-71 ㎛. S. schlegelii (mean weight, 34.55±5.82 g; mean length, 12.59±0.79 cm) were exposed to PE-MPs at concentrations of 0, 400, 800, 1,600 and 3,200 ㎍/L for 10 and 20 days. PE-MPs significantly affected growth performance, hematological parameters, plasma components, and antioxidant responses in a concentration-dependent manner. At a concentration ≥1,600 ㎍/L, PE-MPs significantly decreased body weight gain and specific growth rate, and significantly increased the hepatosomatic index. Hematological parameters showed a significant decrease in total red blood cell count and hemoglobin levels. Plasma components showed a significant increase in glucose, aspartate aminotransferase, and alanine transaminase levels, whereas total protein, calcium, and magnesium levels significantly decreased. Exposure to ≥1,600 ㎍/L PE-MPs also induced reactive oxygen species generation in the gill and liver, significantly increasing superoxide dismutase and catalase activity. These findings suggest that exposure to ≥1,600 ㎍/L PE-MPs could significantly change growth performance, hematological parameters, plasma components, and antioxidant responses, resulting in physiological toxicity.