• The Korean Journal of Malacology
• /
• v.24 no.3
• /
• pp.261-267
• /
• 2008

The tendency of metabolism in oyster, Crassostrea gigas, was investigated in relation to the water temperature and salinity. Oxygen consumption and ammonia excretion were measured and O:N ratio were calculated according to the water temperature from February 2007 to September 2008 and body size. The relationship between oxygen consumption and body weight has been examined in C. gigas. The weight-specific oxygen consumption rate (mg $O_2$/g/h) varied inversely with size. Oxygen consumption and ammonia excretion increased with an increase in water temperature. O:N ratio measured in this study ranged from 8 to 40 under ordinary sea water and the ratio was 8 at $25^{\circ}C$ and 16 at $10^{\circ}C$. This indicates that oyster mainly use the protein as the primary catabolic substrate during gametogenesis. Lower O:N ratio in winter suggests that oysters have to meet their energy demand by metabolizing protein to survive in stressful conditions such as low temperature and lack of sufficient food supply. This studies will provide the basic data for oyster culture farm in assessing the carrying capacity and sustainable management.

• The Korean Journal of Physiology
• /
• v.5 no.2
• /
• pp.29-40
• /
• 1971

Oxygen consumption, pulmonary ventilation, heart rate, and breathing frequency were measured on 8 men walking on a treadmill carrying load of 9 kg on hand, back, or head. Besides measurements were made on subjects carrying loads of 2.6 kg each on both feet. The speed of level walking was 4, 5, and 5.5km/hr and a fixed speed off km/hr with grades of 0, 3, 6, and 9%. Comparisons were made between free walking without load and walking with various types of loads. The following results were obtained. 1. In level or uphill walking the changes in oxygen consumption, pulmonary ventilation, breathing frequency and heart rate were smallest in back load walking, and largest in hand load walking. The method of back load was most efficient and hand load was the least efficient. The energy cost in head load walking was smaller than that of in hand load walking. It was assumed that foot load costed more energy than hand load. 2. In level walking the measured parameters increased abruptly at the speed of 5.5 km/hr. Oxygen consumption in a free walking at 4 km/hr was 11.4ml/kg b.wt., and 13.1 ml/kg b.wt. 5.5 km/hr, and in a hand load walking at 4 km/hr was 13.9, and 18.8 ml/kg b. wt. at 5.5 km/hr. 3. In uphill walking oxygen consumption and other parameters increased abruptly at the grade of 6%. Oxygen consumption at 4 km/hr and 0% grade was 11.4 ml/kg b. wt., 13.6 at 6% grade, and 16.21/kg b. wt. at 9% grade in a free walking. In back load walking oxygen consumption at 4km/hr and 0% grade was 12.3 ml/kg b.wt.,14.9 at 6% grade, and 18.7 ml/kg b.wt. In hand load walking the oxygen consumption was the greatest, namely, 13.9 at 0% grade, 17.9 at 6%, and 20.0 ml/kg b. wt. at 9% grade. 4. Both in level and uphill walking the changes in pulmonary ventilation and heart rate paralleled with oxygen consumption. 5. The changes in heart rate and breathing frequency in hand load were characteristic. Both in level and uphill walk breathing frequency increased to 30 per minute when a load was held on hand and showed a small increase as the exercise became severe. In the other method of load carrying the Peak value of breathing frequency was less than 30 Per minute. Heart rate showed 106 beats/minute even at a speed of 4 km/hr when a load was held on hand, whereas, heart rate was between, 53 and 100 beats/minute in the other types of load carriage. 6. Number of strides per minute in level walking increased as the speed increased. At the speed floater than 5 km/hr number of strides per minute of load carrying walk was greater than that of free walking. In uphill walk number of strides per minute decreased as the grade increased. Number of strides in hand load walk was greatest and back load walk showed the same number of strides as the free walk.

• Journal of Korean Academy of Nursing
• /
• v.13 no.2
• /
• pp.87-104
• /
• 1983

It has been well documented that animals exposed to cold show increased activity of thyroid gland. The calorigenic action of thyroid hormone has been demonstrated by a variety of in vivo and in vitro studies. According to Edelman et al., the thyroid thermogenesis is due to activation of energy consuming processes, especially the active sodium transport by the hormone in target tissues. If so, the increase in thyroid activity during cold exposure should induce increased capacity of sodium transport in target tissue and the change in tissue metabolism should be precisely correlated with the change in Na+_K+_ATPase activity of the tissue. This possibility was tested in the present study: in one series, changes in oxygen consumption and Na+_K+_-ATPase activity of liver preparations were measured in rats as a function of thyroid status, in order to establish the effect of thyroid hormone on the tissue respiration and enzyme system in another series, the effect of cold stimulus on the serum thyroid hormone level, hepatic tissue oxygen consumption and Na+_K+_ATPase activity in rats. The results obtained are as follows: 1. The Na+_dependent oxygen consumption of liver slices, the oxygen consumption of liver mitochondria and the Na+_K+_ATPase activity of liver preparations were significantly inhibited in hypothyroidism and activated in hyperthyroidism. Kinetic analysis indicated that the Vmax. of Na+_K+_ATPase was decreased in hypothyroidism and increased in hyperth)'roidism. 2. In cold exposed rats, the serum triiodothyronine (T₃) level increased rapidly during the initial one day of cold exposure, then declined slowly to the control level after two weeks. The serum thyroxine (T₄) level decreased gradually throughout the cold exposure. Accordingly the T₃/T₄ratio increased. The mitochondrial oxygen consumption and the Na+_dependent oxygen consumption of liver slices increased during the first two days and then remained unchanged thereafter The activity of the Na+_K+_ATPase in liver preparations increased during cold exposure with a time course similar to that of oxygen consumption. Kinetic analysis indicated that the Vmax. of Na+_K+_ATPase increased. 3. Once the animal was adapted to cold, induction of hypothyroidism did not significantly alter the hepatic oxygen consumption and Na+_K+_ATPase activity. These results indicate that: 1) thyroid hormone increases capacities of mitochondrial respiration and active sodium transport in target tissues such as liver; 2) the increased T₃level during the initial period of cold exposure facilitates biosynthesis of Na+_K+_ATPase and mitochondrial enzymes for oxidative phosphorylation, leading to enhanced production and utilization of ATP, hence heat production.

• YAKHAK HOEJI
• /
• v.37 no.6
• /
• pp.647-658
• /
• 1993

The susceptibilities of aldehyde dehydrogenase (AldDH) and alcohol dehydrogenase (ADH) to active oxygen generated by xanthine-xanthine oxidase (XOD) system were studied. Incubation of AldDH with 2$\times$10$^{-3}$ units of XOD for 30 min at $25^{\circ}C$ resulted in the decrease of enzyme activity to 30% and it was inactivated completely when incubated with 5$\times$10$^{-3}$ units of XOD. Whereas 70% of ADH activity was retained after exposure to 5$\times$10$^{-3}$ units of XOD for 30 min, 40% of ADH activity was retained after exposure to 5$\times$10$^{-2}$ unit of XOD for 30 min. This inhibition effect by the active oxygen was preventable by catalase and glutathione, but not by SOD. The rates of the NADPH-dependent oxygen consumption by the liver S-9 mixture and microsomes were also determined in this study. Rate of oxygen consumption is increased in the liver S-9 mix and microsomes from phenobarbital-treated rat, and it was consistent with increased lipid peroxidation. In the presense of ethanol as a substrate, the oxygen consumption rates were increased. It is reported that hepatic AldDH activity is depressed in alcoholic liver diseases, however there is few report that explains the reason of depressed AldDH activity. These results are supportive of the theory that the increase in hepatic ethanol oxidation through the induced ME activity after chronic ethanol feeding generate oxygen radical at elevated rates and it leads to the depression of AldDH activity.

• Journal of the Ergonomics Society of Korea
• /
• v.18 no.1
• /
• pp.1-12
• /
• 1999

The objective of this study was to investigate the metabolic energy consumption rate of the psychophysical Maximum Acceptable Weight of Loads (MAWLs) for different manual materials handling tasks. Lifting activities with four different lifting frequencies (2, 5, 8, 11 lifts/min) for a lifting range (from floor to 76cm height) were studied. The oxygen consumption rate and heart rate were measured or recorded while subjects were lifting their MAWLs. It was found that the relationship between MAWL and frequency can be described best by the exponential function with the R-sq value 0.9865 for this study. Psychophysical MAWL decreased from 22.38 to 7.48 kg, while the oxygen consumption rate with the MAWL increased from 717.8 to $1114.7m{\ell}-O_2/min$ as the frequency increased from 2 to 11 lifts/min. Heart rate also increased from 104.5 to 120.7 bpm. The ratio of oxygen consumption for the MAWL to the Physical Work Capacity (PWC) ranged from 28 to 43%. The MAWLs were greater than or equal to the Maximum Permissible Limits (MPLs) when the frequencies were higher than 8 lifts/min. It seems that the MAWLs by psychophysical approach when the frequencies were higher than 8 lifts/min tend to be overestimated from the viewpoint of the physiological criterion of the oxygen consumption rates. From these findings it is suggested that the NIOSH Guideline should not be directly applied to Korean without reasonable modifications.

• International Journal of Automotive Technology
• /
• v.8 no.1
• /
• pp.119-126
• /
• 2007

Because of the low temperature operation, proton exchange membrane (PEM) fuel cell has a water phase transition. Therefore, water management is an important operation issue in a PEM fuel cell because the liquid water in the fuel cell causes electrode flooding that can lower the cell performance under high current density conditions. In this study, in order to understand the reactant distributions in the cathode channels of the PEM fuel cell, an experimental technique that can measure the species concentrations of reactant gases by using gas chromatograph (GC) is applied for an operating PEM fuel cell. The oxygen distribution along the cathode flow channels of PEM fuel cell is mainly investigated with various operating conditions. Also, the relations between cathode flooding and oxygen concentrations and oxygen consumption pattern along the cathode channel configurations of the unit cell adopted for this study are discussed using GC measurement and visualization experiment of cathode flooding. It is found that the amount of oxygen consumption is very sensitive to various operating conditions of the fuel cell and was much affected by the flooding occurrence in cathode channels.

• Journal of Embryo Transfer
• /
• v.25 no.3
• /
• pp.145-148
• /
• 2010

Oxygen consumption has been regarded as a useful indicator for assessment of mammalian embryo quality. This study was performed to investigate whether oxygen consumption reflects morphological grade of in vivo derived bovine blastocyst-stage embryos (blastocyst). The oxygen consumption of in vitro produced blastocyst was compared to its total cell number. In addition, pregnant rate was measured after transplantation of in vivo blastocysts with different oxygen consumption. The quality of blastocyst collected on day 7 after artificial insemination was categorized as grade I and II (G I and G II) based on microscopic observation of the morphology. Oxygen consumption of blastocyst was measured using a scanning electrochemical microscopy (SECM) and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. Pregnancy of recipient cow was confirmed with rectal palpation after 60 days of embryo transfer. The oxygen consumptions of G I blastocysts were significantly higher than those of G II blastocysts ($10.2{\times}10^{15}/mol\;s^{-1}$ versus $6.4{\times}10^{15}/mol\;s^{-1}$, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7, and 110.2 in the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\sim}10^{15}/mol\;s^{-1}$ respectively. Total cell number was significantly increased in embryos with high oxygen consumption (p<0.05). Pregnant rate in recipient cow was 0, 50, and 85.7% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0, and over $12.0{\times}10^{15}/mol\;s^{-1}$, respectively. These results suggest that measurement of oxygen consumption may help increase the pregnant rate of bovine embryos.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.24 no.5
• /
• pp.303-314
• /
• 1991

Hypoxic bottom $(\leq2.0ml/l),\;40\%\;oxygen\;saturation)$ is formed in the semi-closed Wonmun bay during summer and autumn early. This study was carried out to know seasonal distribution of marine bacteria and the role of marine bacteria for forming the hypoxic bottom at Wonmun bay during summer and autumn early, 1990. During the study periods, 170 bacterial strains were isolated from sea water and sediment. Viable cell counts were ranged between $10^5-10^7\;cells/ml$. The dominant species were Acinetobacter spp. in spring, Flavobacerium spp. in summer, Pseudomonas spp. in autumn, Serratia spp. in winter. Because ETSA(Electron Transport System Activity) reveals potential consumption of oxygen in the aquatic microorganisms, the ETSA was used as potential consumption of oxygen in this study. The potential consumption of oxygen was in the range of $232.4-637.5{\mu}l/O_2/l/day$ by marine organism and $142.6-432.4{\mu}l/O_2/l/day$ by marine bacteria during the study periods. The ratio of potential oxygen consumption of marine bacteria to total marine microorganism was 0.54. The potential consumption of oxygen by marine bacteria closely related with the number of viable cells. Consequently, bacteria play an important role to form Hypoxic bottom at marine environment.

• Physical Therapy Korea
• /
• v.7 no.2
• /
• pp.20-34
• /
• 2000

The purpose of this study was to establish modified physiological cost index (PCI) for predicting energy consumption by heart rate (HR) at isokinetic ergometer exercise testing. The subjects were twenty-eight healthy men in their twenties. All of them performed upper and lower extremity isokinetic ergometer exercise tests which had six loads (400, 500, 600, 700, 800, and 900 kg-m/min) and five loads (400, 500, 600, 700, and 800 kg-m/min) respectively. The exercise sessions were finished when HR was in plateau. HR and oxygen consumption were determined during the final minute. Resting heart rate and oxygen consumption were used for calculating heart rate, oxygen consumption changes and modified PCI. Regression analysis established the relationship between each variable to work load, HR and oxygen consumption. The results were as follows: 1) In the lower extremity ergometer exercise test, oxygen consumption increased continuously as work load increased, but in the upper extremity ergometer test, oxygen consumption only increased until work load was 700 kg-m/min. 2) HR increased as work load increased in both exercise tests, but in the upper extremity ergometer test, HR decreased from the 700 kg-m/min. 3) The modified PCI increased as work load mcreased until the 700 kg-m/min point in the lower extremity ergometer test and until the 500 kg-m/min point in the upper extremity ergometer test when it started to decrease in both tests. 4) In the lower extremity ergometer exercise test, regression analysis established the relation as $dVO_2$ = -.0215HR - .2141 where $dVO_2$ is given in l/min and HR in beat/min ($R^2$ = .2677, p = .000). ln the upper extremity ergometer exercise test. regression analysis established the relation as $dVO_2$ = -.0115HR + .2746 ($R^2$ = .1308, p = .000). The results of this study were similar to previous studies but were different under high work load conditions. So modified PCI should be used with only low intensity work load testing. Subjects for upper extremity ergometer exercise testing should complete a prescribed training course prior to testing, and only low intensity work load should be used for safety considerations.

• The Korean Journal of Physiology
• /
• v.5 no.2
• /
• pp.15-27
• /
• 1971

Physiological analysis of the physical exercise was made on 9 subjects performing mountain climbing. The course between two points (256 and 516 meters altitude) was 1,300 meters in distance and difference of vertical height was 260 meters making the mean grade of 20%. In the field, the heart rates during uphill or downhill walk were recorded by EKG radio-telemetry. In the laboratory, oxygen consumption was obtained by the recorded heart rates, using individual heart rate vs oxygen consumption diagram obtained by treadmill test. the following results were obtained. 1. Uphill walk time was 36.5 minutes, and during this period the mean heart rate was 149.0 heats/min and peak heart rate was 169.2 beats/min. The total heart beats during the uphill walk was 5.433 beats. 2. The ratio of individual mean heart rate during the uphill walk to the maximal heart rate distributed between 66.6% and 98.3%, and the mean of the total group was 83.1%. The ratio of peak heart rate of uphill walk to the maximal heart rate was 94.5% in the group. Thus uphill walk of a 20% grade mountain course was an exhaustive exercise. 3. Oxygen consumption during uphill walk was 2.22 l/min (ranged between 1.79 and 2.70 l/min) and the ratio of this to the resting oxygen consumption was 8.31. The peak value of oxygen consumption during uphill walk was 2.73 l/min and the ratio of this to the resting oxygen consumption was 10.39. 4. Energy expenditure during uphill walk showed a mean of 11.1 kcal/min and the peak expenditure rate was 13.6 kcal/min. The total energy expenditure during 36.5 minutes of uphill walk was 396 kcal. 5. In downhill walk, the time was 31.7 minutes, mean heart rate was 118.4 (ranged between 100.1 and 142.7) beats/min, and the peak heart rate was only 129.4 beats/min. The ratio of mean heart rate to the maximal heart rate was 66.3%. Total heart beats during downhill walk was 3,710 beats. The ratio of downhill oxygen consumption to the resting consumption was 5.70. The rate of energy expenditure was 7.5 kcal/min, and the total onery expenditure during the 31.7 minutes of downhill walk was 228 kcal. 6. The effect of training was manifest in the uphill walk and not in the downhill walk. After training in mountain course walk, i) the uphill time was shortened, ii) mean heart rate increased, iii) time vs heart rate curve became smooth and showed less frequent zig-zag, i.e., the depth of trough on the curve decreased and the magnitude was less than 10 beats. In non-trained subject the depth of trough on the curve was greater than 50 beats and appeared more frequently. 7. Mountain climbing is a good health promotion exercise. For the promotion of health the reasonable amount of uphill mountain walk exercise in a 20% grade course is a walk for 40 or 50 minutes duration once a week.